High-performance Liquid Chromatographic Analysis for Quantitation of Marker Compounds of Artemisia capillaris Thunb

( Kyung Min Park ),( Ying Li ),( Bora Kim ),( Haiyan Zhang ),( Kyong Hwangbo ),( Dong Gen Piao ),( Mei Juan Chi ),( Mi Hee Woo ),( Jae Sue Choi ),( Je Hyun Lee ),( Dong Cheul Moon ),( Hyeun Wook Chang 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0

Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among `Injinho` and `Myeon-injin` and `Haninjin`--A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as `Injinho` in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration andquantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognitionanalysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.

사료원료 공급량 및 스크류 회전속도를 달리하여 제조한 배합사료가 넙치(Paralichthys olivaceus)의 성장 및 체조성에 미치는 영향

김경덕 ( Kyoung Duck Kim ),김강웅 ( Kang Woong Kim ),이봉주 ( Bong Joo Lee ),배기민 ( Ki Min Bae ),안철민 ( Cheul Min An ),한현섭 ( Hyun Sob Han ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.5

The aim of this study was to investigate the effect of diet extruder conditions, such as feed-loading rate and screw speed, on growth performance and biochemical responses in olive flounder Paralichthys olivaceus. Over 8 wks, we used four identical diets (triplicated per treatment) with differing ratios of feed-loading rate (kg/h):screw speed (rpm/min) in a laboratory-scaled twin-screw extruder of 50:640, 80:640, 120:640, and 80:400, designated as EP1, EP2, EP3, and EP4, respectively. Screw speed impacted the buoyancy of experimental diets. Diets produced at a screw speed of 640 rpm/min floated for > 24 hrs, whereas those produced at a speed of 400 rpm/min sank between 10 s and 5 min. Fish that were fed EP1 and EP4 diets grew significantly faster than those fed EP2 and EP3 diets. Fish fed EP1 diets ate and gained weight most efficiently among treatments, a result that is likely to be related to feed-loading rate, i.e., ingredients extruded at a low feed-loading rate may have more time to cook in the pre-conditioner of the extruder. A cooked diet may be easier to digest in fish. Fish fed EP4 diets also showed significant weight gain, as compared to those fed EP2 and EP3 diets. However, we found no differences among treatments in proximate compositions of dorsal muscle, liver, and viscera of fish. Our results suggest that extruder conditions, may influence feed quality, impacting feed efficiency and growth of fish.

Propionibacterium freudenreichii KFCC 31227과 Lactobacillus acidophilus KFCC 32825의 혼합배양에 관한 연구(1) : 유기산 생성을 위한 균주의 생육특성

閔潤植,金奇哲 충북대학교 농업과학기술연구소 1992 農業科學硏究 Vol.10 No.1

Prpionibacterium sp. and Lactobacilus sp. were cultured in single and mixed under the condition of S. G. Y. medium with skim milk added with glucose and yeast extract or skim milk whey, and compared conditions of fermentation and acid fermentation by interaction. Propionibacterium 3 strains and Lactobacilus 6 strains, the best strains in growth or organic acid production were P. freudenrecichii KFCC 31227 and L. acidophilus KFCC 32825 which have shown typical symbiosis and commensalism tendency. P. freudenreichii KFCC 31227 and L. acidophilus KFCC 32825 showed better growth in mixed cultuure than in single culture. Production of lactic acid was more in single culture and the production of propionic acid was increased in mixed culture. The optimum condition of acid production was approximate pH 7, temp. 35℃, and 72 culture hr. The heating condition of medium was 100℃, 30 min. The optimum ratio of two kinds of strain starter was 1 : 1.

Interleukin-5 저해 활성 chalcone 화합물의 유효 입체 구조

이기철,김민석,P. Thanigaimalai,Vinay K. Sharma,박경래,김영수,정상헌 충남대학교 약학대학 의약품개발연구소 2010 藥學論文集 Vol.25 No.-

Interleukin(IL)-5 appears to be one of the main proinflammatory mediators among the growing number of cytokines and chemokines that induce eosinophilic inflammation. Previously, our group synthesized a number of chalcone derivatives to determine their inhibitory activity against IL-5. However, the structure-activity relationship (SAR) of these chalcones was somewhat unclear, robably due to presence of three free rotatable bonds. To further, explore the SAR of these chalcones, we synthesized eight 2-(benzylidene)-2,3-dihydroinden-1-ones and 2- benzylidene)-3,4-dihydronaphthalen-1(2H)-ones 6 and evaluated for their inhibitory activity against IL-5. These rigidified chalcones 6 exhibited very weak inhibitory activity against IL-5 compared to chalcones 2. The structures of 6 closely resemble to the stretched conformations of chalcone 2, not effective conformation for the inhibition on IL-5 function.

소형 기기용 효율적인 바이트 코드 검증기술에 관한 연구

황철준,조증보,정민수 慶南大學校 附設 工業技術硏究所 2003 硏究論文集 Vol.21 No.2

USIM(Universal Subscriber Identity Module) 기술은 데이터의 관리와 보호를 하나의 소형 칩 내에 집약시킨 기술로 최근에 이 기술에 대한 관심도가 증가하고 있다. 자바카드 가상기계는 자바 언어로 작성된 프로그램이 USIM 칩 내부에서 실행 가능하도록 해준다. 하지만 자바카드 가상 기계는 자원 제약적인 디바이스의 특성으로 인하여 검증기술을 제공하지 않는다. 본 논문에서는 정적 체크 기반의 효율적인 바이트코드 검증기를 제안한다. 연구 결과 본 논문에서 제안하는 검증기는 일반적인 것에 비해 90% 이상의 사이즈가 감소되었다. As Universal Subscriber Identity Module (USIM) technology can handle, manager and protect data within a small sized chip, peopled are more interested in USIM technology lately. Java Card Virtual Machine (JCVM) enables programs written by Java programming language to be run in smart cards with USIM chips. However JCVM does not have a verifier as limits of hardware. In this paper, we propose a ByteCode verifier with novel algorithm based on Static Check policy. We experiment that the size of our verifier is reduced by 90% over generals.

WBI 교수모형을 이용한 페트리 네트 학습의 설계 및 구현

김경민,노철우 신라대학교 교육과학연구소 1999 교육과학연구 Vol.- No.4

황놀래기,Pseudolabrus japonicus (HOUTTUYN)의 生殖周期와 性轉換

安哲民,李榮敦,李定宰,李澤烈 濟州大學校海洋硏究所 1992 해양과환경연구소 연구논문집 Vol.16 No.-

1990년 12월부터 1991년 10월까지, 그리고 1992년 7월부터 11월까지 제주도 북방합덕연안을 중심으로 채집된 황놀래기, Pseudolabrus japonicus를 대상으로 그들의 生殖生態를 파악하기 위하여 體色變化와 生殖巢熟度指數, 그리고 生殖巢의 조직학적 방법에 의한 生殖週期, 性轉換 過程등을 조사한 결과는 다음과 같다. 1. 생식소 숙도지수(GSI)는 암수 다같이 8월에 접어들면서 상승하기 시작하며 10월에 최대 치를 나타내고, 12월에 접어들면서 하강하기 시작하여 1월부터 낮은 값을 나타내고 있다. GSI최대값은 암컷 2.32(±0.35), 수컷 0.38(±0.18)을 나타냈다. 2. 生殖週期는 成長期(8∼9월), 成熟期(9∼10월), 完熟 및 産卵期(10∼12월), 回復 및 休止期(12∼7월)등의 연속적인 주기를 나타냈다. 3. 體長 11.5∼16.0㎝되는 개체들에서 雌性에서 雄性으로 性轉換 단계의 生殖巢를 갖고 있다. 4. 본 種은 1차정소와 2차정소가 혼합되어 나타나는 複雄性의 雌性完熟魚에 속하는 것으로 나타났다. 5. 암컷에서 수컷으로 性轉換 함에 따라 적색에서 청색으로 體色變化가 나타나고 있다 Reproductive cycle and sex reversal of Pseudolabrus japonicu s were investigated histologically on the gonadal development, sex change of the gonad and studied by gonadosomatic index(GSI), color patterns. Sample were collected at the costal area of Ham duck in Cheju-do, from December, 1990 to November 1992. In male and female, GSI began to increase from August and reached it's maximum value in October, respectively. It began to decrease from December, the low temperature season, thereafter, maintained relatively low values until July of next year. The annual reproductive cycle of this species could be classified into four sucessive developmental stage : growing stage(August-September), mature stage(September-October), ripe and spent stage(October-December), degenerative and resting stage(December-July). The species is a diandric, protogynous hermaphrodite, and has two color patterns: red-yellow (A) and dark-green (B). A-fish were less than 14.5㎝ SL and consisted of females (92.7%), secondary male(80.2%), primary male(5.5%), female(5.5%) and fish with transitional gonad(8.8%). In P.japonicus, individuals with hermaphrodic gonad were observed in 11.5∼16.0㎝ SL. Sex reversal of this species could be occured from 11.5 to 16.0㎝ SL.

21세기 대안적 발전 패러다임과 지방분권

김영철,이민환 慶北大學校 經濟經營硏究所 2002 經商論集 Vol.30 No.1

마르코프 reward 모델을 이용한 통신망의 성능 및 신뢰도 분석 기법

노철우,김경민 신라대학교 자연과학연구소 1998 自然科學論文集 Vol.5 No.-

This paper describes techniques to analyze network reliability and performance using Markov Reward Models(MRM). MRM is obtained by associating reward rates with Markov chain's states. This process facilitates combined evaluation of performance and reliability. Stochastic Reward Net(SRN) is a formalism for a high level specification of MRM. Modeling techniques to construct SRN network reliability models with comparing GSPN models are given. It is shown that reward-based models could lead to more concise model specification and solution of a variety of new measures. With respect to the solution of performance and reliability models, we propose two practical approaches: hierarchichal and phase type expansion modeling and show how reliability/availability measures can be obtained via performance analysis using special reward rates assignment. Two software packages, SPNP and SHARPE, that allow the specification and solution of MRMs are used.

해상풍력 하부 구조물용 강재의 정전류 부식 시험 시 전류밀도 인가 시간이 부식손상에 미치는 영향

이정형(Jung-Hyung Lee),박재철(Jae-Cheul Park),한민수(Min-Su Han),장석기(Seok-Ki Jang),김성종(Seong-Jong Kim) 한국표면공학회 2016 한국표면공학회지 Vol.49 No.5

This research investigated the relationship between the corrosion damage characteristics of offshore wind steel substructure and the time of current density application by electrochemical accelerated short-term corrosion test. The galvanostatic corrosion was conducted on the steel specimens in natural seawater with a constant current density ranging from 1 ㎃/㎠ to 200 ㎃/㎠ for 1 ~ 180 min. Macro and micro observation was carried out on the surface of the corrosion damaged area using SEM and 3-dimensional analysis microscope. The weight loss of the specimens before and after was calculated as the difference between the initial weight prior to corrosion and weight after removal of the corrosion product. It was shown that during galvanostaic corrosion process, the corrosion behavior could be characterized by the onset of pitting corrosion in the early stage and the uniform corrosion in the late stage, showing damage development in the depth direction with the time of current application. The result of the 3D analysis revealed that both damage depth and surface roughness increased with increasing time of current application. The weight loss curves with time showed that a coefficient of determination (R²) was relatively high for the relationship between the time of current application and weight loss. As a result, the degree of corrosion can be controlled by simply varying the time of current application.