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A routing protocol based on Context-Awareness for Energy Conserving in MANET
Chen, Yun,Lee, Kang-Whan The Korea Institute of Information and Commucation 2007 Journal of information and communication convergen Vol.5 No.2
Ad hoc networks are a type of mobile network that function without any fixed infrastructure. One of the weaknesses of ad hoc network is that a route used between a source and a destination is to break during communication. To solve this problem, one approach consists of selecting routes whose nodes have the most stable link cost. This paper proposes a method for improving the low power distributed MAC. This method is based on the context awareness of the each nodes energy in clustering. We propose to select a new scheme to optimize energy conserving between the clustering nodes in MANET. And this architecture scheme would use context-aware considering the energy related information such as energy, RF strength, relative distances between each node in mobile ad hoc networks. The proposed networks scheme could get better improve the awareness for data to achieve and performance on their clustering establishment and messages transmission. Also, by using the context aware computing, according to the condition and the rules defined, the sensor nodes could adjust their behaviors correspondingly to improve the network routing.
Optimization of Medium Components for Plasmid Production by Recombinant E. coli DH5α pUK21CMVβ1.2
Yinghua Lu,Shuzhen Zheng,Karl Friehs,Ning He,Xu Deng,Qingbiao Li,Zheng He,Chenyun Xu 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.3
In this study we investigated the effects of carbon and nitrogen sources, and the carbon to nitrogen ratio (C:N) of growth medium on the production of plasmid DNA by pUK21CMVβ1.2 harbouring recombinant E. coli. The results indicated that sucrose and casein peptone were the most suitable carbon and nitrogen sources, respectively, for plasmid production, and the plasmid concentration was significantly influenced by the C:N ratio. A Box-Behnken design and surface response methodology were employed to formulate the optimal medium. In the optimized medium, a plasmid concentration of 51.80 mg/L and a plasmid selectivity of 23.49 mg/g were achieved after 24 h of incubation. These values were approximately 3.5 and 1.7 times higher, respectively, than those obtained on the original LB medium.
Zheng, Shuzhen,Friehs, Karl,He, Ning,Deng, Xu,Li, Qingbiao,He, Zheng,Xu, Chenyun,Lu, Yinghua Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.3
In this study we investigated the effects of carbon and nitrogen sources, and the carbon to nitrogen ratio (C:N) of growth medium on the production of plasmid DNA by $pUK21CMV{\beta}1.2$ harbouring recombinant E. coli. The results indicated that sucrose and casein peptone were the most suitable carbon and nitrogen sources, respectively, for plasmid production, and the plasmid concentration was significantly influenced by the C:N ratio. A Box-Behnken design and surface response methodology were employed to formulate the optimal medium. In the optimized medium, a plasmid concentration of 51.80mg/L and a plasmid selectivity of 23.49mg/g were achieved after 24h of incubation. These values were approximately 3.5 and 1.7 times higher, respectively, than those obtained on the original LB medium.