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Stochastic Modeling of External Electric Field Effect on Escherichia Coli Min Protein Dynamics
Charin MODCHANG,Wannapong TRIAMPO,Paisan KANTHANG,Udorn JUNTHORN,Somrit UNAI,Waipot NGAMSAAD,Narin NUTTAVUT,Darapond TRIAMPO,Yongwirnon LENBURY 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.2
Cel division in Escherichia coli and other rod-shaped bacteria depends on the precise place- ment of a division septum at the cel center. The MinCDE system consisting of thre proteins, MinC, MinD, and MinE, controls acurate cel division at the center of the cel through pole-to- pole oscilation. With simplifying asumptions and relying on a deterministic model, we present a one-dimensional stochastic model that describes the effects of an external electric field on the MinCDE system. Computer simulations were performed to investigate the response of the oscila- tory dynamics to various strengths of the electric field and to the total number of Min proteins. A sufficient electric field strength was capable of interfering with MinCDE dynamics with posible changes to the cel division proces. Interestingly, effects of an electric field were found not to depend on the total number of Min proteins. The noise involved shifted the corect trend of Min proteins behavior. However, as a consequence of the robustnes of the dynamics, the oscilatory patern of the proteins stil existed even though the number of Min proteins was relatively low. When considering the corelations betwen the local and the global minimum (maximum) of MinD (MinE), the results suggest that using a high enough Min protein concentration wil reduce the localminimum(maximum)effect, which is related to the probability of polar division in each single oscilator cycle. Although this model is simple and neglects some complex mechanisms concerning protein oscilation in corelation with celdivision, it has ben demonstratedto be goodenough for positioning of the dividing site. Nevertheles, more experimental and theoretical studies are neded to provide a more realistic (but of course more complicated) model of bacterial cel division.
Wannapong Triampo,Charin Modchang,I-Ming Tang,Narin Nuttawut,Paisan Kanthang,Waipot Ngamsaad,Yongwimol Lenbury 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.4
Determining the middle of the bacteria cell and the proper placement of the septum is essential to the division of the bacterial cell. In E. coli, this process depends on the proteins MinC, MinD, and MinE. Here, the lattice Boltzmann method (LBM) is used to study the dynamics of the oscillations of the min proteins from pole to pole. This determines the midcell division plane at the cellular level. The LBM is applied to the set of eterministic reaction diffusion equations proposed by Howard et al. to describe the dynamics of the Min proteins. The LBM results are in good agreement with those of Howard et al. and agree qualitatively with the experimental results. Our good results indicate that the LBM can be an alternative computational tool for simulating problems dealing with complex biological systems that can be described by using the reaction-diffusion equations
An Ising-like Model for Monolayer-monolayer Coupling in Lipid Bilayers
Kan Sornbundit,Charin MODCHANG,Narin NUTTAVUT,Waipot Ngamsaad,Darapond TRIAMPO,Wannapong TRIAMPO 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.1
We have proposed the Ising bilayer model to study the domain growth dynamics in lipid bilayers. Interactions within and between layers are adopted from recent experimental and theoretical data. We investigate the effects of the mismatch area on the domain coarsening dynamics in both symmetric and asymmetric lipid bilayers. To explore domain coarsening, we used the Monte Carlo (MC) method with a standard Kawasaki dynamics to simulate the systems. The results show that domains on both layers grow following a power-law and that the domains grow slower when the mismatch areas are increased.
Pinpanit Boonchuay,Charin Techapun,Phisit Seesuriyachan,Thanongsak Chaiyaso 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5
Production of xylooligosaccharides (XOs)from corncob using the thermostable endo-xylanase fromStreptomyces thermovulgaris TISTR1948 was investigatedusing KOH pretreatment, followed by enzymatic hydrolysis. The optimal reaction time for production of XOs was 12 h,after which xylobiose comprised a majority of products,and a low xylose content was observed. The optimalconditions for production of XOs were studied using acentral composite design. At an enzyme concentration of129.43 U/g of substrate, 53.80oC, and pH 6.17, the yield ofXOs reached 162.97 mg/g of substrate or 752.15 mg/g ofhemicellulose in KOH-pretreated corncob. The prebioticproperties of XOs derived from corncob were alsoinvestigated using in vitro fermentation of those XOs withthe known probiotic strains Lactobacillus casei TISTR1463,L. lactis TISTR1464, and L. plantarum TISTR1465. XOsderived from corncob were comparable to commercialXOs for an ability to enhance the growth of the specifiedprobiotic lactobacilli.
Gene Expression Profiling of Intrahepatic Cholangiocarcinoma
Subrungruang, Ittisak,Thawornkuno, Charin,Chawalitchewinkoon-Petmitr, Porntip,Pairojkul, Chawalit,Wongkham, Sopit,Petmitr, Songsak Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1
Intrahepatic cholangiocarcinoma (ICC) is ranked as one of the top five causes of cancer-related deaths. ICC in Thai patients is associated with infection with the liver fluke, Opisthorchis viverrini, but the molecular basis for development remains unclear. The present study employed a microarray approach to compare gene expression profiles of ICCs and normal liver tissues from the same patients residing in Northeast Thailand, a region with a high prevalence of liver fluke infection. In ICC samples, 2,821 and 1,361 genes were found to be significantly up- and down-regulated respectively (unpaired t-test, p<0.05; fold-change ${\gep}2.0$). For validation of the microarray results, 7 up-regulated genes (FXYD3, GPRC5A, CEACAM5, MUC13, EPCAM, TMC5, and EHF) and 3 down-regulated genes (CPS1, TAT, and ITIH1) were selected for confirmation using quantitative RT-PCR, resulting in 100% agreement. The metallothionine heavy metal pathway contains the highest percentage of genes with statistically significant changes in expression. This study provides exon-level expression profiles in ICC that should be fruitful in identifying novel genetic markers for classifying and possibly early diagnosis of this highly fatal type of cholangiocarcinoma.
Wannapong Triampo,Ankana Boondirek,Charin Modchang,I-Ming Tang,Narin Nuttawut,Paisan Kanthang,Suchitra Sanguansin,Waipot Ngamsaad,Yongwimol Lenbury 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.4
One of the most important steps in the developmental process of the bacteria cells at the cellular level is the determination of the middle of the cell and the proper placement of the septum, these being essential to the division of the cell. In E. coli, this step depends on the proteins MinC, MinD, and MinE. Exposure to a constant electric field may cause the bacteria’s cell-division mechanism to change, resulting in an abnormal cytokinesis. To see the effects of an external field e.g., an electric or magnetic field on this process, we have solved a set of deterministic reaction diffusion equations, which incorporate the influence of an electric field. We have found some changes in the dynamics of the oscillations of the min proteins from pole to pole. The numerical results show some interesting effects, which are qualitatively in good agreement with some experimental results.
Kotepui, Manas,Thawornkuno, Charin,Chavalitshewinkoon-Petmitr, Porntip,Punyarit, Phaibul,Petmitr, Songsak Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11
Breast cancer is the leading cause of cancer deaths among women worldwide, including Thailand. In the present study, the differential mRNA expression of SVEP1, LPHN3, KLB, ITGA7, SEMA3G, TNS1 and MMP13 genes was examined in breast cancer using quantitative real-time reverse transcription polymerase chain reaction (QRT-PCR). Among these genes, increased LPHN3 and MMP13 mRNA expression levels correlated with axillary-node metastasis (P=0.02). Multiple logistic regression analysis revealed that LPHN3 and MMP13 mRNA expression is significantly associated with axillary node status in breast cancer (P=0.04).
SLC35B2 Expression is Associated with a Poor Prognosis of Invasive Ductal Breast Carcinoma
Chim-ong, Anongruk,Thawornkuno, Charin,Chavalitshewinkoon-Petmitr, Porntip,Punyarit, Phaibul,Petmitr, Songsak Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15
Background: Breast cancer is the most common malignancy in women worldwide, including Thailand, and is a major cause of mortality and morbidity, despite advances in diagnosis and treatment. Novel gene expression in breast cancer is a focus in searches for prognostic biomarkers and new therapeutic targets. Materials and Methods: The mRNA expression of novel B4GALT4, SLC35B2, and WDHD1 genes in breast cancer were examined in invasive ductal breast carcinoma (IDC) patients using quantitative real-time reverse transcription polymerase chain reaction (QRT-PCR). Results: Among these genes, increased expression of SLC35B2 mRNA was significantly associated with TNM stage III + IV of IDC (p<0.001). Hence, up-regulation of SLC35B2 may serve as a prognostic biomarker for poor prognosis, and is also a potential therapeutic target in breast cancer.
Hudrudchai Suchitra,Suwanwong Charin,Prasittichok Pitchada,Mohan Kanu Priya,Janeaim Nopphadol 대한예방의학회 2024 예방의학회지 Vol.57 No.1
Objectives: The effectiveness and efficiency of pre-exposure prophylaxis (PrEP) in reducing the transmission of human immunodeficiency virus (HIV) among men who have sex with men (MSM) relies on how widely it is adopted and adhered to, particularly among high-risk groups of MSM. The meta-analysis aimed to collect and analyze existing evidence on various factors related to PrEP adherence in MSM, including demographic characteristics, sexual behaviors, substance use, and psychosocial factors.Methods: The meta-analysis followed the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. The search included articles published between January 2018 and December 2022, obtained from the PubMed, ScienceDirect, and Scopus databases. The studies that were included in the analysis reported the proportion of MSM who demonstrated adherence to PrEP and underwent quality appraisal using the Newcastle-Ottawa Scale.Results: Of the 268 studies initially identified, only 12 met the inclusion criteria and were included in the final meta-analysis. The findings indicated that education (odds ratio [OR], 1.64; 95% confidence interval [CI], 1.12 to 2.40), number of sexual partners (OR, 1.16; 95% CI, 1.02 to 1.31), engaging in sexual activities with an human immunodeficiency virus-positive partner (OR, 1.59; 95% CI, 1.16 to 2.26), substance use (OR, 0.83; 95% CI, 0.70 to 0.99), and lower levels of depression (OR, 0.55; 95% CI, 0.37 to 0.82) were associated with higher rates of PrEP adherence among MSM.Conclusions: Despite these findings, further research is necessary to investigate PrEP adherence more comprehensively. The findings of this meta-analysis can be utilized to inform interventions aimed at improving PrEP adherence among MSM and provide directions for future research in this area.