Human Foamy Virus Bel1 Protein Produced in Escherichia coli and Insect Cells is Biologically Functional

Chang, Jun,Sung, Young Chul Korean Society of Molecular Biology 1995 Molecules and cells Vol.5 No.4

Intensified solvent extraction and separation of cobalt from Ni-rich leaching solution in impinging stream-rotating packed bed contactor

Chang Jun,Jia Fukang,Mumford Kathryn A.,Yang Xiaohong,Xie Zinan 한국자원공학회 2020 Geosystem engineering Vol.23 No.5

This work describes the separation of Ni and Co from a real feed solution in chloride media with high Ni content. The extractant used was P507 (2-ethylhexyl phosphoric acid-2-ethylhexyl ester) in a laboratory scale Impinging Stream–Rotating Packed Bed (IS-RPB) contactor. Key variables including feed solution pH, extractant concentration, saponification degree, high gravity level and total volumetric flow rate of fluids amongst other variables were examined. A McCabe–Thiele diagram of the extraction stage predicted that at a total volumetric flow rate of 90 L/h, feed solution pH of 5, saponified P507 concentration of 25% and high gravity of 83, almost 99% of cobalt, would be extracted within three stages. With regard to the scrubbing results, the co-extracted nickel was scrubbed efficiently from the loaded organic solution using a mixture of 0.02 M CoCl2 and 0.5 M hydrochloric acid solution, The purified Co solution was achieved after stripping the loaded-organic phase with 1 M HCl. The maximum value of the overall volumetric mass transfer coefficient for the IS-RPB extractor was found to be 0.16 s−1 which was superior to that of conventional extractors such as mixer-settler and centrifugal extractors, providing strong confidence for its industrial application.

Single mucosal immunization of recombinant adenovirus-based vaccine expressing F1 protein fragment induces protective mucosal immunity against respiratory syncytial virus infection

Chang, Jun,Jang, Ji Eun,Yu, Jae Rang,Kim, Sol 梨花女子大學校 藥學硏究所 2011 藥學硏究論文集 Vol.- No.21

Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract disease in infancy and early childhood. Despite its importance as a pathogen, there is no licensed vaccine against RSV. The fusion (F) protein of RSV is a potentially important target for protective antiviral immune responses. Here, we studied the immune responses elicited by recombinant replication-deficient adenovirus (rAd)-based vaccines expressing the soluble F1 fragment of F protein (amino acids 155-524) in murine model. The expression of secreted F1 fragment by rAd was significantly increased by codon optimization. Strong mucosal IgA response was induced by single intranasal immunization of codon-optimized vaccine, rAd/F1co, but not by rAd/F1wt. A single intranasal immunization with rAd/F1co provided potent protection against subsequent RSV challenge. Interestingly, neither serum Ig nor T-cell response directed to F protein was detected in the rAd/F1co-immune mice, suggesting that protective immunity by rAd/F1co is mainly mediated through mucosal IgA induction. Indeed, co-delivery of cholera toxin B subunit significantly enhanced mucosal IgA responses by the optimized vaccine, which correlates with protective efficacy. Taken together, our data demonstrate that a single intranasal administration of rAd/F1co is sufficient for the protection and represents a promising prophylactic vaccination regimen against RSV infection.

Efficient amplification of melanoma-specific CD8^(+)T cells using artificial antigen presenting complex

Chang, Jun 이화여자대학교 약학연구소 2008 藥學硏究論文集 Vol.- No.17

In vitro large amplification of tumor-specific cytotoxic T lymphocytes (CTLs) and adoptive transfer of these cells is one of the most promising approaches to treat malignant diseases in which an effective immune response is not achieved by active Immunization. However, generating sufficient numbers of tumor-specific CTLs stimulated with autologous antigen presenting cells (APCs) in vitro is one of the most problematic steps in the adoptive cell transfer (ACT) therapy. To circumvent this problem, we have developed an artificial antigen presenting Complex (aAPCs) using MHC class 1 molecules loaded with a melanoma-specific TRP-2 peptide epitope. Our results show that TRP-2-specific CD8^(+) T cells elicited by immunization with recombinant adenovirus expressing the mini-gene epitope are efficiently stimulated and amplified in vitro to a greater extent by aAPCs than by natural splenic APCU. These aAPC-induced CTLs recognized endogenously pro - cessed antigens present on B16F10 melanoma cells. Efficient stimulation and proliferation of antigen- specific T cells was also confirmed using ovalbumin peptide-loaded aAPCs and OT-I TCR transgenic cells. These results demonstrate that prior in vivo immunization, which increases the precursor frequency, simplifies posterior expansion of tumor- specific CD8_(+) T cells, and aAPCs is superior to autologous APC for in vitro amplification. This ""prime and expand"" regimen can be an alternative method for large amplification of rare tumor-specific CTLs and aAPCs should be a useful tool for ACT immunotherapy.

Intranasal Delivery of Cholera Toxin Induces Th 17- Dominated T-Cell Response to Bystander Antigens

Chang, Jun,Lee, Jee Boong,Jang, Ji Eun,Song, Man Ki 이화여자대학교 약학연구소 2010 藥學硏究論文集 Vol.- No.20

Cholera toxin (CT) is a potent vaccine adjuvant, which promotes mucosal immunity to protein antigen given by nasal route. It has been suggested that a promotes T helper type 2 (Th2) response and suppresses Th1 response. We here report the induction of Th17-dominated responses in mice by intranasal delivery of CT. This dramatic Th17-driving effect of CT, which was dependent on the B subunit, was observed even in Th1 or Th2-favored conditions of respiratory virus infection. These dominating Th17 responses resulted in the significant neutrophil accumulation in the lungs of mice given CT. Both in vitro and in vivo treatment of CT induced strongly augmented IL-6 production, and Th17-driving ability of CT was completely abolished in IL-6 knockout mice, indicating a role of this cytokine in the Th17-dominated T-cell responses by CT. These data demonstrate a novel Th17-driving activity of CT, and help understand the mechanisms of a adjuvanticity to demarcate Thelper responses.

MHC multimer: A Molecular Toolbox for Immunologists

Chang Jun 한국분자세포생물학회 2021 Molecules and cells Vol.44 No.5

The advent of the major histocompatibility complex (MHC) multimer technology has led to a breakthrough in the quantification and analysis of antigen-specific T cells. In particular, this technology has dramatically advanced the measurement and analysis of CD8 T cells and is being applied more widely. In addition, the scope of application of MHC multimer technology is gradually expanding to other T cells such as CD4 T cells, natural killer T cells, and mucosalassociated invariant T cells. MHC multimer technology acts by complementing the T-cell receptor-MHC/peptide complex affinity, which is relatively low compared to antigen-antibody affinity, through a multivalent interaction. The application of MHC multimer technology has expanded to include various functions such as quantification and analysis of antigen-specific T cells, cell sorting, depletion, stimulation to replace antigen-presenting cells, and single-cell classification through DNA barcodes. This review aims to provide the latest knowledge of MHC multimer technology, which is constantly evolving, broaden understanding of this technology, and promote its widespread use.

Development of Operator Training System in Governor Station by Integrating Process and Accident Simulation in Real Time

Changjun Ko(고창준),Yongseok Lee(이용석),Chonghun Han(한종훈) 한국가스학회 2016 한국가스학회 학술대회논문집 Vol.2016 No.6

Triphenylamine-Based Supramolecular Polymers for Conductive Gel

Changjun Park,Jisung Kim,Jinhee Lee,Sang Youl Kim 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

Analysis of Green Area Ratio by Industrial Complex Type to Minimize DEFORESTATION

Changjun Kim,Hyangju Lee,Wonhyeon Lim J-INSTITUTE 2021 Public Value Vol.6 No.3

Purpose: As the economy grows, many forests, fields, and agricultural land have been converted into industrial complexes. Accordingly, there are deep concerns about damage to the natural ecosystem and environmental im-pact. This study analyzed the factors related to the natural environment by type for industrial complexes promot-ed in forest areas. The authors hope to balance development and conservation by identifying the relationship between factors related to the natural environment. In addition, this study tried to derive rational ways to develop industrial complexes suitable for low-carbon green growth. Method: This study was analyzed based on 95 environmental impact assessments that business operators ap-plied for consultation with agencies concerned to build industrial complexes in Daegu and Gyeongbuk, Korea, for 16 years from 2005 to 2020. The researchers conducted a one-way ANOVA analysis to determine the difference in green area ratio, forest field ratio, tree transplantation ratio, and ecological area ratio by industrial complex type . Post-hoc analysis was performed by the Scheffe test. This paper used the Spss Statistics 21 statistics program to conduct the above statistical analysis. Results: The number of consultations for industrial complexes was highest for general industrial complexes. The green area ratio was 5.60% higher in national industrial complexes than in agricultural and industrial com-plexes. The forest field ratio was 71.84% in quasi-industrial complexes, 40.14% higher than 31.70% in quasi-agro-industrial complexes. The tree transplantation ratio was low in national industrial complexes and quasi-industrial complexes. The ecological area ratio decreased by 72.93% after development compared to before development in general industrial complexes and decreased by 65.75% after development compared to before development in national industrial complexes. Conclusion: Before the industrial complex development, the forest field ratio showed differences by type. How-ever, there was no difference in the ecological area ratio after the development of the industrial complex. This result was seen as the minimum standard set by law in parks and green areas. Therefore, it is necessary to develop eco-friendly industrial complexes rather than reckless development to minimize forest damage. The authors be-lieve it is necessary to respond to climate change and contribute to low-carbon green growth by minimizing the forest area within the development area and increasing the ecological area ratio and tree transplantation ratio after development.

Abnormal state accident scenario generation & validation in pressure regulatory station by process simulator

Changjun Ko(고창준),Yeongsuk Lee(이용석),Changhwan Heo(허창환),Chonghun Han(한종훈) 한국가스학회 2015 한국가스학회 학술대회논문집 Vol.2015 No.11