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Changhoo Chun,Haeyoung Na 한국원예학회 2011 원예과학기술지 Vol.29 No.5
Cold pretreatment, washing medium and composition of nutrient media may have marked effects on microspore embryogenesis. When microspores isolated from radish (Raphanus sativus L. cv. Gwanhun) flower buds were washed with Nitsch & Nitsch (NLN) medium liquid medium containing 130 g·L-1 sucrose (NLN-13), yields of microspore-derived embryos were greater than when using B5 liquid medium containing 130 g·L-1 sucrose. Microspore viability is known to decrease rapidly with storage; however, in this experiment, microspore viability was maintained for 24 h at 4°C without media. Among the various medium concentrations used (0.25*, 0.5*, 1.0*, 2.0*, and 4.0* NLN liquid medium), 0.5* NLN liquid medium induced the most efficient formation of microspore-derived embryos. In addition, microspore-derived embryos yields were greater when microspores were cultured in 0.5* NLN liquid medium supplemented with 0.25*, 0.5*, and 1.0* NLN microelements, compared to medium not supplemented with microelements. In this study, the highest yield of microspore-derived embryos was observed when the microspores derived from flower buds were washed using NLN-13 liquid medium and then cultured on 0.5* NLN liquid medium supplemented with 0.25* NLN microelements, followed by incubation at 25°C for 30 days.