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Changhai Wang,Yingying Sun,Ronglian Xing,Liqin Sun The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2
For more accurately describing the durations of the light and the dark phases of micro-algal cells over the whole light-dark cycle, and probing into the relationship between the liquid circulation time or velocity, the aeration rate and cell density, a series of experiments was carried out in 10 cm light-path flat plate photobioreactors. The results indicated that the liquid flow in the flat plate photobioreactor could be described by liquid dynamic equations, and a high biomass output, higher content and productivity of arachidonic acid, $70.10\;gm^{-2}d^{-1},\;9.62\%$ and 510.3 mg/L, respectively, were obtained under the optimal culture conditions.
Changhai Wang,Yiyun Wang,Qiao Su,Xiaorong Gao 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.2
A transient expression system for a unicellular marine green alga, Chlorella sp. MACC/C95, was developed using a reporter GUS gene coded for by plasmid pBI121. The results demonstrated a high transformation efficiency could be achieved by using electroporation to deliver DNA into intact cells and the CaMV35S promoter to drive the foreign gene expression in Chlorella sp. MACC/C95. The use of a carrier DNA coupled with osmosis treatment improved the transformation efficiency, while linearization of the plasmid had minor effects. Investigation of the effects of DNA concentration and growth phases of Chlorella sp. MACC/C95 on transformation efficiency indicated that the highest level of transient expression was observed when 6 μg mL-1 of plasmid DNA and cells 2~6 days old were used.
Changhai Chen,Xi Zhu,Hailiang Hou,Lijun Zhang,Xiaole Shen,Ting Tang 국제구조공학회 2014 Steel and Composite Structures, An International J Vol.16 No.3
Experiments were carried out to investigate the ballistic performance of fiber reinforced plastic(FRP)-steel plates completely penetrated by hemispherical-nosed projectiles at sub-ordnance velocities greater than their ballistic limits. The FRP-steel plate consists of a front FRP laminate and a steel backing plate. Failure mechanisms and impact energy absorptions of FRP-steel plates were analyzed and compared with FRP laminates and single steel plates. The effects of relative thickness, manufacturing method and fabric type of front composite armors as well as the joining style between front composite armors and steel backing plates on the total perforation resistance of FRP-steel plates were explored. It is found that in the case of FRP-steel plates completely penetrated by hemispherical-nosed projectiles at low velocities, the failure modes of front composite armors are slightly changed while for steel backing plates, the dominate failure modes are greatly changed due to the influence of front composite armors. The relative thickness and fabric type of front composite armors as well as the joining style of FRP-steel plates have large effects whereas the manufacturing method of front composite armors has slight effect on the total perforation resistance of FRP-steel plates.
Wang, Changhai,Wang, Yiyun,Su, Qiao,Gao, Xiaorong Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.2
A transient expression system for a unicellular marine green alga, Chlorella sp. MACC/C95, was developed using a reporter GUS gene coded for by plasmid pBI121. The results demonstrated a high transformation efficiency could be achieved by using electroporation to deliver DNA into intact cells and the CaMV35S promoter to drive the foreign gene expression in Chlorella sp. MACC/C95. The use of a carrier DNA coupled with osmosis treatment improved the transformation efficiency, while linearization of the plasmid had minor effects. Investigation of the effects of DNA concentration and growth phases of Chlorella sp. MACC/C95 on transformation efficiency indicated that the highest level of transient expression was observed when $6{\mu}g\;mL^{-1}$ of plasmid DNA and cells $2{\sim}6$ days old were used.
Wang Changhai,Sun Yingying,Xing Ronglian,Sun Liqin 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2
For more accurately describing the durations of the light and the dark phases of microalgal cells over the whole light-dark cycle, and probing into the relationship between the liquid circulation time or velocity, the aeration rate and cell density, a series of experiments was carried out in 10 cm light-path flat plate photobioreactors. The results indicated that the liquid flow in the flat plate photobioreactor could be described by liquid dynamic equations, and a high biomass output, higher content and productivity of arachidonic acid, 70.10 gm-2d-1, 9.62% and 510.3 mg/L, respectively, were obtained under the optimal culture conditions.
Trichromatic Dyeing of Polyimide Fiber Using Its Inherent Color as a Yellow Component
Dongyan Shao,Changhai Xu,Jinmei Du,Hongbo Wang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.8
Polyimide (PI) fiber is one kind of high-performance fibers with outstanding thermal stability, mechanicalproperties, and chemical resistance, but it is difficult to be dyed due to its tightly packed macromolecules and lack offunctional groups for dyeing. Especially, the inherent yellow shade of PI fiber limits the color gamut that can be matched bytrichromatic dyeing. In this study, it is proposed to investigate the possibility to dye PI fibers into a black shade with dispersedyes and basic dyes with the assistance of swelling agents. The dyeing experiments showed that, among the proposedswelling agents, N-methyformanilide (MFA) was the preferred candidate for enhancing the dyeing ability of PI fibers. Usingthe inherent yellow color of PI fiber as a yellow component in trichromatic dyeing it could be dyed into a black shade withgood colorfastness to washing, rubbing, and dry-heating. In spite of causing a slight decrease in tensile strength the dyeingwould not impair the thermal stability of PI fibers.
Changming Wang,Xinyu He,Changhai Yan,Wende Lü,Weizhi Sun 한국지질과학협의회 2013 Geosciences Journal Vol.17 No.2
The Lengshuibeigou Pb-Zn-Ag deposit is located in the Qinling superlarge metallogenic belt, China. The Pb-Zn-Ag orebodies are hosted in the Proterozoic Luanchuan Group. The ore types are hydrothermal veins and skarns type. The ore-forming process can be divided into three stages: (1) quartz-pyrite; (2) quartz-sulphide; and (3) quartz-carbonate. This paper used stable and radiogenic isotopes to study the characteristics of the Lengshuibeigou Pb-Zn-Ag deposit. The temperature and salinity of fluids tend to decrease from the early skarn and quartz- pyrite stages to the late quartz-carbonate stage. The ranges of 18OH2O (SMOW) and DH2O (SMOW) values of the ore-forming fluids range from –0.03 to +4.97‰ and from –83.00 to –72.00‰, respectively. The 34SV-CDT values of pyrite, sphalerite and galena from the skarn-type and hydrothermal-type vein ores range from +0.34 to +5.88‰, which is similar to those of porphyry-type Mo-W deposits in the area, but is different from those in the strata. The sulphur in both types of ores may have been derived from magmatic sources. The ranges of 206Pb/204Pb, 207Pb/204Pb and 208Pb/204Pb ratios in the sulphide ores are 17.457–17.765, 15.423–15.606 and 37.712–39.012, respectively, which are comparable with those of feldspars in porphyry granites in the Nannihu ore field and less radiogenic than those in the host strata. These new data support the likelihood that the fluids and metals associated with Lengshuibeigou Pb-Zn-Ag deposit were carried by the porphyry magmatic system. The 40Ar/39Ar plateau age of 137.9 ± 0.4 Ma age obtained from the Lengshuibeigou Pb-Zn-Ag deposit is likely its mineralization age. This paper is useful not only for interpretation of the genesis of the magmatic-hydrothermal deposits but also significant for mineral exploration in the area especially for finding large deposits in large magmatic districts where various types of base metal deposits might occur.