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어린이 체험은 어떻게 형상화되는가? -임길택 동시를 중심으로-
염창권 ( Changgwon Yeom ) 건국대학교 동화와번역연구소 2016 동화와 번역 Vol.31 No.-
어린이글에 나타난 체험의 양상을 ‘어린이 생활현실’로, 임길택 동시에 나타난 양상을 ‘작품 속 허구적 현실’로 가정하여 작가의 형상화 과정에서 개입될 수 있는 요소들을 귀납하여 보았다. 연구 결과는 다음 세 가지로 정리할 수 있다. 첫째, 서술대상에 대한 인식적인 측면에서 차이가 발생한다. 어린이글에서 주체는 경험적 한계 내에서 자신을 직접적, 자연발생적으로 드러낸다면, 작가의 글에서는 작가의 세계관과 주제의식의 영향을 받는다. 이때, 어린이들의 행동과 경험세계는 선택되어 간접화된 방식으로 전달된다. 둘째로, 어린이글에 나타난 체험의 양상은 범주성의 제약을 받는 다. 작가의 글에서는 두 겹의 의미 층위를 발견할 수 있는데, 어린이 체험을 선택하는 과정에서 1차적 의미가 발생하고 이를 다시 주제의식에 결부시켜 해석할 때 2차적 의미가 발생한다. 셋째로, 임길택 동시에 나타난 체험은 어린이글에 나타난 양상과 유사하거나 근접해 있다. 이것은 제재 자체를 통해 발휘되는 현장성 또는 진정성의 국면이다. 임길택의 동시에는 모두 당위의 세계가 제시된다는 점에서, 작가의 세계관을 “어린이의 체험의 건강성을 긍정하는”, ‘현실적 이상주의’로 명명할 수 있다. In this study, I focused on the differences between the life reality and the fictional reality. The elemnents involved in the process of the imagery are reasoned inductedly by comparing the writings of the children and those of the writers. Results can be summarized into the following three. First, the difference occurs in the cognitive aspects of the subject for description. In the writings of the children, the subject reveals itself directly and spontaneously. In the writings of the writers, it is affected by the outlook and subject consciousness of the writers. At this point, the behavior and experience world of the children are selected and transferred indirectly. Secondly, experience apearing at the writings of the children has the restrictions of the spectrum. The writings of the writers have two layers of meaning. The primary meaning occurs in the process of the children``s experience selection. The secondly meaning occurs when the first meaning is interpreted with the subject consciousness. Third, Im Giltaek’s Poetry similarly or closely represents children``s experience with the writings of the children. This is the reality or the truth displayed by the subject itself. Im Giltaek’s works can be named as “Affirming the healthy experience of children,” ‘realistic idealism’. Because all his writings suggest the desirable world.
Early Pregnancy Diagnosis in Dairy Cattle – a Proteomic Approach
Hyun-Joo Lim,Ho-Beak Yoon,Changgwon Dang 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
High reproductive efficiency is a prerequisite for dairy animals in order to optimize dairy production. An accurate and early pregnancy diagnosis is a crucial aspect for better reproductive management in livestock. The indigenous, visual and clinical methods have various limitations including accuracy, sensitivity, specificity, later stages of applicability and requires highly skilled manpower. This hurdles the researchers to ensue further research on developing novel early pregnancy diagnostics for dairy animals. However, the advantage of molecular techniques like proteomics has given a new hope to look for pregnancy biomarkers in animal research. In this study, we assayed the pregnancy-associated glycoprotein (PAG) levels using anti-bPAG antibody. Serum plasma samples were collected 30, 60, 90 and 120 days after insemination. Cattle diagnosed pregnant with singleton are used for this experiment in which blood samples were collected. The plasma PAG level is gradually increased after insemination to until 60 days and drastically decreased after that. The PAG level was approximately 2-fold greater during 30 days compared with 60 days. These results concluded to validate our other pregnancy diagnosis methods including circulating microRNA and cell free DNA concentration (data not shown). Despite this proteomic approach, validating all other molecular technique results may give the exact time for early pregnancy diagnosis.
Sharma, Aditi,Lee, SeungHwan,Lee, JunHeon,Dang, Changgwon,Kim, Hyeong Cheul,Yeon, SeongHum,Kang, HeeSeol,Kanwar, Shamsher Singh,Vijh, Ramesh Kumar Institute of Agricultural Science 2014 Korean Journal of Agricultural Science Vol.41 No.4
The evolutionary history of cattle and buffalo has always been a topic of great interest to the evolutionary biologists. The phylogenetic studies of bovin species has been carried out at various levels, varying from the study of domestication and migration of populations to major cladogenesis. Along with the archeological studies there are studies from molecular biology and more recently from genomics. The phylogenetic perspective of the bovins and their evolutionary history, are reviewed in terms of what has been done, what needs to be done and potential challenges in doing it.
Aditi Sharma,SeungHwan Lee,JunHeon Lee,Changgwon Dang,Hyeong Cheul Kim,SeongHum Yeon 충남대학교 농업과학연구소 2014 농업과학연구 Vol.41 No.4
The evolutionary history of cattle and buffalo has always been a topic of great interest to the evolutionary biologists. The phylogenetic studies of bovin species has been carried out at various levels, varying from the study of domestication and migration of populations to major cladogenesis. Along with the archeological studies there are studies from molecular biology and more recently from genomics. The phylogenetic perspective of the bovins and their evolutionary history, are reviewed in terms of what has been done, what needs to be done and potential challenges in doing it.
Fetal Sex Determination in Dairy Cattle using DNA Isolated from Maternal Plasma
Hyun-Joo Lim,Hyun Jong Kim,Ji Hwan Lee,Soo Bong Park,Seung Min Ha,Dong Hyun Lim,Tae IL Kim,Min Su Kim,Kyung Suk Lee,Changgwon Dang 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
The development of methodological strategies that allow for the prediction of the fetal sex in cattle still remains a zoo-technical challenge. So far, different methods have been implemented to direct the breeding management in dairy cattle, giving producers an advantage in decision-making regarding the activity of planning and monetary gains. The objective of this study was to evaluate the use of next generation sequencing of fetal DNA in the maternal plasma of pregnant cattle in order to determine the sex of the fetus. The whole blood samples were collected from the five Holstein cows at 20-week of pregnancy. The serum plasma was harvested and the cell free DNA (cfDNA) was isolated in order to construct whole genome DNA library. The resulting DNA libraries were then paired-end sequenced (2×101 bp) with Illumina’s HiSeq 2500 instrument. The high quality, clean reads were mapped to the Bovine reference UMD 3.1 of X and Y chromosome separately, resulting an average depth and coverage of 3.54 and 86.2%. Our results revealed that four dairy cattle with male fetuses and 1 with a female fetus. Furthermore, it was concluded that fetal cells in maternal plasma can be used to predict the sex of the fetus in cattle. The detection of fetal DNA sequences is a reality and could reduce the risk of invasive techniques for certain fetal disorders in the near future.