치아를 이용한 법의치과학적 개인식별

윤창률 大韓法醫學會 1999 대한법의학회지 Vol.23 No.2

Dental examination proves to be one of the most effective means of identification available. Restorations, type, location, and configuration may be highly specific. In addition, teeth and restorative materials are relatively nonperishable. Because dental identification appears to be one of the better methods, particularly in the badly burned, decomposed, sklotonized, dr macerated bodies, a dentist is often called upon to aid the identification. Depending on the degree of experience and sophistication of the police investigator, it is not infrequent that they sit back and wait for the dentist to make an identification.

울진군 후포리에서 발굴된 치아에 관한 연구

김종열,윤창륙 大韓法醫學會 1992 대한법의학회지 Vol.16 No.1

The teeth are important object of study in forensic odontology. With regard to ancient Korean teeth of Hupori, the authors studied the size and morphology of the teeth, dental caries and degree of attrition and determined the sex and age. The results are as follows: 1. In the morphology of teeth, there was little difference between the Hupori teeth and the moderan human teeth, but in Hupori teeth, the shovelling of lingual fossa of mixillary incisors were prominent. 2. In comparision of crown size, there was little difference between the modern human teeth and Hupori teeth, crown wide of maxillary incisors and mandibular first molars in Hupori teech were slightly greater than the modern human teeth. 3. In the examination of the dental caries, 7 out of 23 permanent teeth examined were decayed, but none of 14 deciduous teeth. 4. The degrees of teeth atrition were mostly slight. 5. As a result of sex and age determination, the proportion of males to females was similar, the age of teeth was identified as twenties mostly.

무수치 상아질에서 Amelogenin gene 및 Short Tandem Repeat(STR) 유전좌위 F13A01, LPL 분석

김남리,윤창륙,김세연 大韓法醫學會 1998 대한법의학회지 Vol.22 No.1

Theoretically, if the odontoblastic layer on the pulpal surface of the dentin is removed, it is impossible to find nucleus after the teeth has undergone endodontic therapy. So dentin does not contain genomic DNA. But it is in existence the possibility that blood permeates into the dentin by endodontic therapy and trauma. Therefore DNA in blood can be detected from the dentin layer. The purpose of this investigation is to evaluate the possibility of individual identification after detection of DNA in the dentin of endodontic created teeth. The authors isolated the human DNA from 40 endodontic treated teeth, performed AmpFLPs by PCR and electrophoresed for detection of STR loci F13A01, LPL gene and X-Y homologous amelogenin gene. The following results were obtained: 1) DNA extraction was possible in 19 endodontic treated teeth out of 40. 2) Sex determination was possible in 12 endodontic treated teeth out of 40 by detection of X-Y homologous amelogenin gene 3) F13A01 locus was detected in 6 endodontic treated teeth out of 40, observed 4 alleles(3.2, 4, 5, 6) and 5 genotypes(3.2-3.2, 3.2-4, 3.2-5, 4-5, 6-6). 4) LPL locus was detected in 7 endodontic treated teeth out of 40, observed 3 alleles(10, 11, 12) and 3 genotypes(10-10, 10-12, 11-12). From the above results DNA extraction, sex determination, amplification of STR locus F13A01, and LPL gene were possible in the endodontic treated teeth and it was suggested that endodontic treated teeth were useful and applicatable as molecular biological samples for individual identification.

선천성 매독환자의 치아 및 악안면 기형에 대한 임상적 연구

안종모,윤창륙 朝鮮大學校 口腔生物學硏究所 1991 Oral Biology Research (Oral Biol Res) Vol.15 No.-

A femle patient, 11 year-old, who referred from Department of orthodontics due to suggested congenital syphilis by Hutchinson incisor, and Hutchinson's incisor, mulberry molar, perioral rhagades and saddle nose, mandibular protuberance was examed clinically. Radiographs showed developing third molars and notch-shaped maxillary central incisors. Postitive reaction was observed by TPHA test & FTA-ABS test. According to the clinical, radiographical & clinicopathological findings, final diagnosis was established as late congenital syphilis. Late congenital syphilis is a rare transplacental infectious disease by infected materity with the common characteristic of marked frontal bossing, Hutchinson's teeth, interstitial ketatitis, 8th nerve deafness, saddle nose, mulberry molar, rhagades, saber shin, Clutton's joint etc. Patient was cared by administration of erythromycin per oral, but continuous careful follow-up was recommended for confirmation of serologic negative change.

삼풍백화점 붕괴 대참사의 법의치과학적 개인식별 : Using the Forensic Odontological Studies

김종열,이영석,윤창륙,허웅,최종훈,김재홍 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

하악과두 골절에 대한 임상적 연구

손보형,이효빈,황호길,윤창륙,여환호 大韓顎顔面成形再建外科學會 1992 Maxillofacial Plastic Reconstructive Surgery Vol.14 No.4

The author investigated the 162 patients with the condylar fractres of the mandilble who were admitted in Dept. of Oral and Maxillofacial Surgery, Hospital of Chosun Dental School from January 1985 to September 1992 clinically, including fracture incidennce, age and sex of the patients, causes of injures, associated injures, treatments and complications and obtained the following results. 1. Of the 449 patients with mandibular fractures, 162patients suffered condylar fractures(36.1%). 2. Of the 162 patients, males with condylar fractures were 128 patients, by a ratio of 3.8 : 1. 3. The most frequently affected age group was the third decade(32.7%). 4. Falls were ranked as the predominant cause(50.6%), followed by traffic accident(27.8%) and violence (16.0%). 5. The incidence of single condylar fractures was 28% and symphysis fractures were the most commomest of the concomittant injuries(60%). 6. The subcondylar fractures occurred most frequently(41.6%) and anteromedial displacement of the condylar fragments occurred most frequently(45.4%) 7. Of the all condylar fracture patients, children under 15 of age comprized 31 patients(19.1%) and condylar head fractures occurred most frequently at those children. 8. Of the managetments in condylar fractures, open reduction was 52.5% and remainder were closed reduction(47.5%). 9. Complications ensured such as TMJ ankylosis, limitation of mouth opening and mandibular movements, TMJ dysfunction, and anterior open bite.

치아를 이용한 성별검사 및 D1S80 유전자위의 검색시 4 가지 DNA 추출방법에 따른 비교

허웅(Woong Hur),윤창륙(Chang Lyuk Yoon) 조선대학교 구강생물학연구소 1995 Oral Biology Research (Oral Biol Res) Vol.19 No.2

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운동강도와 지속시간에 따른 트레드밀 운동이 비만 쥐의 심장근 내 항산화에 미치는 영향

김명희,김용억,윤창륙,유지원,안종모,Kim, Myung-Hee,Kim, Young-Eok,Yoon, Chang-Lyuk,Ryu, Ji-Won,Ahn, Jong-Mo 대한안면통증구강내과학회 2013 Journal of Oral Medicine and Pain Vol.38 No.1

트레드밀 운동이 비만 쥐의 심장근 내 항산화에 어떠한 영향을 주는지 알아보고자 흰쥐에 6주 동안 고지방 식이로 비만을 유발시킨 뒤 운동강도와 적용시간을 달리하여 트레드밀을 3주 동안 적용하였으며, 체중변화, 혈중지질성분, 조직학적 변화, 심장근 내 항산화 효소와 MCP-1(monocyte chemoattractant protein-1) 등의 변화를 연구하였다. 4주령 Sprague Dawley계 수컷 흰쥐 32마리를 사용하여 각각 일반사료를 섭취한 정상군 (normal), 비만 대조군 (control), 비만 쥐에게 고강도 분할운동을 적용한 실험군 I(Experimental I), 비만 쥐에게 중강도 지구력 운동을 적용한 실험군 II (Experimental II)로 나누었다. 실험 결과 첫째, 체중변화는 고강도 분할 운동을 적용한 실험군 I과 중강도 지구력 운동을 적용한 실험군 II는 대조군에 비해 유의한 감소가 있었다 (p<0.001). 특히, 운동을 적용한 1주에서 3주까지 유의한 차이가 있었다. 둘째, 혈중지질성분 수치는 고강도 분할 운동을 적용한 실험군 I과 중강도 지구력 운동을 적용한 실험군 II는 대조군에 비해 총콜레스테롤, 중성지방, 저밀도 지단백질의 유의한 감소, 고밀도 지단백질에서는 유의한 증가가 있었다 (p<0.001). 셋째, 심장근의 조직학적 소견은 심장근에서 아교질 형성과 평활근 섬유의 정렬 상태와 밀집도, 심장근 세포크기가 중강도 지구력 운동 적용한 실험군 II에서 정상군과 비슷한 형태인 근육들이 잘 밀집해 있는 것을 볼 수 있었다. 또한, 섬유아세포, 염증세포들의 침윤 등이 고지방 식이 유발 쥐인 대조군과 고강도 분할 운동을 적용한 실험군 I에서 가장 심하였으며, 중강도 지구력 운동을 적용한 실험군 II는 대조군에 비해 섬유아세포, 염증세포들의 침윤 등이 현저히 감소하였다. 넷째, 심장근의 항산화 효소 SOD(superoxide dismutase), CAT(catalase), GPx(glutathione peroxidase) 수치는 중강도 지구력 운동을 적용한 실험군 II와 고강도 분할 운동을 적용한 실험군 I에서 모두 유의한 증가가 있었다 (p<0.001). 다섯째, 심장근의 항산화 단백질 MCP-1 발현 수치는 고강도 분할 운동을 적용한 실험군 I과 중강도 지구력 운동을 적용한 실험군 II 모두에서 감소가 있었으며 (p<0.01), 특히, 중강도 지구력 운동을 적용한 실험군 II에서는 유의한 감소가 있었다 (p<0.001). 따라서, 중강도 지구력 운동은 고지방 식이로 유발된 비만 쥐의 심장근 내 항산화에 영향을 주어 비만 개선과 산화적 스트레스에 의한 심장근육 손상 감소 등에 효과적인 것으로 나타났다. The aims of this study was to observed an effect of antioxidative in cardiac muscle of high fat diet induced obesity rat by treadmill exercise with intensity and time. Thirty-two Sprauge-Dawley rats which were divided into four group. Normal, Control(high fat diet induced obesity rat), Experimental I(high intensity intermittent exercise in high fat diet induced obesity rat), Experimental II(moderate intensity endurance exercise in high fat diet induced obesity rat). The results of this study were as follows: 1. In change of body weight, the outcome of each group significantly difference compared with control. Also, 1 to 3 weeks significantly different compared with pre valu experimental I and II(p<0.001). 2. In change of lipid profile, the outcome of each group significantly difference compared with control(p<0.001). Difference between experimental I and II is not significantly. 3. In change of antioxidative enzymes(SOD, CAT, GPx) in myocardium, there are significant difference between control and experimental II, and also between control and experimental I(p<0.001). 4. In change of antioxidative protein MCR-1, the outcome of each group significantly difference compared with control(p<0.01). Experimental II was most significantly difference than the other group(p<0.001). The above results suggest that treadmill exercise effectively reduced in fat. It would be considered that moderate intensity endurance exercise has an effects on improved antioxidative enzyme in cardiac muscle of high fat diet induced obesity rat.

타액내 혈형물질 분비형 및 비분비형에 따른 이하선 타액내 Pr , Db , Pa 단백질의 유전자 빈도타액내 혈형물질 분비형 및 비분비형에 따른 이하선 타액내 Pr , Db , Pa 단백질의 유전자 빈도

김산(San Kim),윤창륙(Chang Lyuk Yoon) 조선대학교 구강생물학연구소 1994 Oral Biology Research (Oral Biol Res) Vol.18 No.2

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Age estimation by pulp/tooth area ratio in a Korean sample on digital panoramic radiographs

( Byung-yoon Roh ),( Chang-gyum Kim ),( Sang-seob Lee ),( Won-joon Lee ),( Yo-seob Seo ),( Ji-won Ryu ),( Jong-mo Ahn ),( Chang-lyuk Yoon ) 조선대학교 치의학연구원(구 조선대학교 구강생물학연구소) 2020 Oral Biology Research (Oral Biol Res) Vol.44 No.4

Age estimation based on the change in the size of the pulp due to secondary dentin deposition can be conducted non-invasively using radiographic photographs. Among these methods, age estimation using the pulp/tooth area ratio is highly accurate. The present study was conducted to apply this method using the panoramic radiographs of a Korean sample population and to evaluate its applicability in the Korean population. The study was conducted using panoramic radiographic images of 349 Koreans aged 20-79 years. The pulp/ tooth area ratio was calculated by measuring the area of the pulp and tooth. Teeth with a single canal, such as maxillary central incisor, lateral incisor, canine, and mandibular canine, were selected. Correlations between this ratio and age were calculated and regression formulas were derived. Age and pulp/tooth area ratio showed relatively low correlations compared with other studies. In particular, men showed lower correlations than women, and upper teeth showed a higher correlation than lower teeth. The regression formulas derived in this study also showed higher errors than those reported in other studies. Age estimation using pulp/tooth area ratio in a Korean using digital panoramic radiographs showed larger errors when compared with previous studies. Morphological diversity of tooth and pulp, indistinct anatomical details in panoramic radiograph, reduction in crown size due to attrition, and ethnic differences are believed to have affected the results.