아세틸 콜린 유발 검사시 허혈성 심전도 변화와 흉통의 발생이 내피세포 기능장애에 미치는 영향

최철웅(Cheol Ung Choi),나승운(Seung-Woon Rha),김선원(Sun Won Kim),나진오(Jin Oh Na),임홍의(Hong Euy Lim),김진원(Jin Won Kim),김응주(Eung Ju Kim),한성우(Seong Woo Han),박창규(Chang Gyu Park),서홍석(Hong Seog Seo),오동주(Dong Joo Oh) 대한임상노인의학회 2010 대한임상노인의학회지 Vol.11 No.4

연구배경: 아세틸 콜린 유발 검사시 발생하는 흉통과 허혈성 심전도 변화가 아세틸 콜린에 의해 발생하는 관상동맥의 내피세포 기능에 어떤 영향을 주는지 알아보고자 했다. 방법: 흉통으로 내원하여 관상동맥 조영술과 아세틸 콜린 유발검사를 시행받은 1,085명의 환자를 대상으로 하였다. 아세틸 콜린 유발 검사를 하는 동안 허혈성 심전도 변화 및 흉통의 발생 유무에 따른 관상동맥 내피세포 기능의 특징을 비교하였다. 결과: 539명의 환자에서 아세틸 콜린 검사시 내피세포 기능 장애가 발생 하였다. 허혈성 심전도 변화가 있었던 그룹이 심전도 변화가 없었던 그룹보다 흉통이 더 많았고(78.1% vs. 60.8%, P=0.007), 관상동맥 조영술상 다발성 관상동맥 경련이 빈번하였고(59.4% vs. 40.6%, P=0.004), 관상동맥의 경련 정도도 더 심하였다(64.1% vs. 46.5%, P=0.006). 흉통이 있었던 그룹이 흉통이 없었던 그룹보다 허혈성 심전도 변화의 빈도가 더 높았다(14.7% vs. 7.5%, P=0.007). 또한 흉통이 있었던 그룹이 관상동맥 경련이 다발성으로 발생하는 경우가 많았고 (50.7% vs. 29.5%, P<0.001), 미만성으로 발생하는 경우가 많았고(87.1% vs. 75%, P<0.001), 관상동맥의 경련의 정도도 더 심하였다(51.6% vs. 43.5%, P=0.041). 결론: 아세틸 콜린 유발검사시 발생하는 허혈성 심전도 변화와 흉통은 관상동맥 경련의 정도와 경련이 발생하는 관상동맥 개수와 연관이 있다. Background: ECG changes and chest pain during acetylcholine (Ach) provocation tests may constitute important clinical parameters for Ach-induced endothelial dysfunction. We investigated the association between ischemic ECG changes and chest pain during the Ach-provocation test and angiographic characteristics of Ach-induced endothelial dysfunction. Methods: A total of 1,085 patients with anginal symptoms underwent diagnostic coronary angiography (CAG) and Ach-provocation tests. We compared angiographic characteristics of Ach-induced endothelial dysfunction according to the presence of ECG change and chest pain. Results: A total of 539 patients experienced Ach-induced endothelial dysfunction. Patients who experienced ECG change group suffered more frequent chest pain (78.1% vs. 60.8%, P=0.007), angiographically more frequent multiple coronary artery spasm (59.4% vs. 40.6%, P=0.004), and more severe coronary artery spasm (64.1% vs. 46.5%, P=0.006) than patients without ECG change. However, there was no difference in the length of endothelial dysfunction between the groups. The incidence of ischemic ECG changes in patients with chest pain was higher than in patients without chest pain (14.7% vs. 7.5%, P=0.007). Patients who experienced chest pain more often experienced multiple (50.7% vs. 29.5%, P<0.001), diffuse (87.1% vs. 75%, P<0.001), and severe coronary artery spasm (51.6% vs. 43.5%, P=0.041) than patients without chest pain. Conclusion: Ischemic ECG changes and chest pain during the Ach-provocation test are associated with multi-vessel involvement and severe coronary artery spasm. Chest pain is associated with the length of endothelial dysfunction, but ischemic ECG change is not.

Long second toe: its nail and skin changes

( Geun-hwi Park ),( Woo-il Kim ),( Min-young Yang ),( Won-ku Lee ),( Tae-wook Kim ),( Sung-min Park ),( Hyun-joo Lee ),( Gun-wook Kim ),( Hoon-soo Kim ),( Hyun-chang Ko ),( Byung-soo Kim ),( Moon-bum 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Nail can be affected by trauma, infection, and systematic disorders. In particular, the toe nails are exposed to the repeated and cumulative minor traumas, which usually result in thickened, splitted, and discolorated nails. Considering these, it can be inferred that the long second toe can be related to various nail and skin changes by repeated minor traumas such as friction in shoes, but the study or report on this topic hasn’t been done yet. Objectives: The purpose of this study was to introduce various nail and skin changes on long second toe. Methods: We analyzed the kinds of nail/skin changes of 50 patients with long second toe, enrolled at Pusan National University Hospitals (Busan and Yangsan) from 2013 to 2018. Results: There was no significant difference in the incidence of left and right toe and toenail, and 7 (14%) patients presented bilateral distribution. The most common nail change on long second toe was melanonychia (23, 46%), followed by subungual hematoma (15, 30%), onycholysis (2, 4%), subungual hyperkeratosis (2, 4%), onychomadesis (1, 2%), and retronychia (1, 2%). The most common skin change was corn (9, 18%), followed by paronychia (2, 4%). Twenty (20/50, 40%) patients also had other toe or toenail changes. Among them, the most common site was big toe/nail (13, 62%), and the most common change was subungual hematoma (8, 42%). Conclusion: Through this study, the dermatologists can recognize and diagnose the nail and skin changes of long second toe.

개에서 동소성 간이식술시의 대사 및 혈역학적 변화

이창준,홍순용,신근만,최영룡,이영주,문종선 대한마취과학회 1993 Korean Journal of Anesthesiology Vol.26 No.3

Skilled and experienced anestheia is of great importance for patients undergoing orthotopic liver transplantation, because of multiple preexisting medical problems in such patients as well as the intraoperative problems of rapid hemodynamic, metabolic, and coagulation changes. In this study, the intraoperative hemodynamic and laboratory data were analyzed in ten dogs that underwent an orthotopic liver transplantation procedure by veno-venous bypass using Biopump. Liver transplantation can be divided into three distinct periods: stage I, or preanhepatic stage, which begins with the induction of anesthesia and continues until cross clamping of portal vein and IVC; stage II, or anhepatic stage, which begins at the anhepatic time and continues until the donor liver is reperfused by the recipients circulating blood; and stage III, or postanhepatic stage, which begins at the time of reperfusion and continues until the end of surgical procedure. The hemodynamic changes at the time of IVC and portal vein cross clamping were decreases in CVP, PCWP, and pulmonary artery pressure in spite of using Biopump. The significant metabolic alternations during anhepatic stage were decrease in blood glucose levels and increase in blood lactate levels. The more significant hemodynamic changes occurred at the time of reperfusion. Systolic pressure decreased suddenly to 58±6 mmHg and cardiac output decreased to 1.08±0.1l L/min. However heart rate, pulmonary artery pressure, CVP, and PCWP did not change significantly. During stage III, hyperglycemia occurred quite frequently. Significant abnormal coagulation chages could not be found, probably because the dogs were healthy. In conclusion, during anhepatic stage, we have to compensate for alternations of fluid balance. At the time of reperfusion, we should prevent severe hemodynamic changes and treat them immediately if they occur. However, it seems that glucose administration is not necessary to the liver recipient during stage II because there is no significant hemodynamic depression e to hypoglycemia at this time and hyperglycemia occurs later

COLOR STABILITY OF CURRENT PROSTHETIC COMPOSITES UNDER ACCELERATED AGING AND IMMERSION IN A COFFEE SOLUTION

Kim, Hyo-Jin,Heo, Seong-Joo,Koak, Jai-Young,Chang, Ik-Tae The Korean Academy of Prosthodonitics 2002 대한치과보철학회지 Vol.40 No.3

The color stability of current prosthetic composites is unknown, even though the quality of composite materials has been improving. This study examined the intrinsic color stability of various current prosthetic resins (ceramic-polymers) after an accelerated aging process and the extrinsic color stability after immersion in a coffee solution. By comparing the amount of discoloration after aging with that without aging, the effect of the accelerated aging process on external discoloration could be evaluated. Three current prosthetic composites (Artglass, Targis, Sculpture), one light polymerized direct composite (Z100) and one dental porcelain control (Ceramco) were assessed. The color changes (${\Delta}$E) of all the specimens were determined using the CIE $L^{*}a^{*}b^{*}$ color order system with a reflected spectrophotometer. The results were as follows: 1. The prosthetic composite materials subjected to the accelerated aging test showed no significant difference in color changes (p >.05). 2. In the coffee solution immersion test after the aging process, the color changes of the Targis and Artglass groups were not different from that of the Z100 group, which showed the highest color change. 3. In the immersion only test, a significantly high color change was observed in the sculpture glazing group. 4. The aging process influenced on the color changes more in the Targis, Artglass and Z100 groups than in the Sculpture and Ceramco groups.

암세포 증식에 대한 YB-1 안티센스 올리고핵산염의 영향

김명성 ( Myung Sung Kim ),이완식 ( Wan Sik Lee ),박창환 ( Chang Hwan Park ),주영은 ( Young Eun Joo ),김현수 ( Hyun Soo Kim ),최성규 ( Sung Kyu Choi ),유종선 ( Jong Sun Rew ),정영도 ( Young Do Jung ),김세종 ( Sei Jong Kim ),안봉환 대한내과학회 2006 대한내과학회지 Vol.71 No.3

목적: YB-1은 PCNA, DNA 중합효소 및 MDR 유전자 등의 전사인자로 작용한다. YB-1 유전자는 정상 어른의 간에서는 발현되지 않지만 태아의 간이나 재생 중인 간에서는 발현이 현저히 증가되어서 세포의 증식과 밀접한 관련이 있음이 보고되었다. 본 연구에서는 YB-1 유전자의 안티센스 올리고핵산염을 이용하여 YB-1의 발현을 억제함으로써 암세포 증식을 차단 할 수 있는지를 실험하고 암치료를 위한 유전자 요법으로서의 가능성을 알아보았다. 방법: 세포주로는 Chang liver, HepG2, CT-26 세포를 사용하였고, 사람의 정상세포로는 섬유아세포와 내피세포가 혼재된 조직을 사용하였다. YB-1 유전자의 안티센스 올리고핵산염으로는 YB-1 cDNA의 변역 시작 부위에 상보적인 21mer 올리고핵산염을 제작하여 사용하였다. 세포의 성장은 MTT assay를 이용하였고, 유전자 발현은 Northern blot으로 분석하였으며, 세포주기 변화는 propidium iodide로 염색하여 유식세포분석기로 분석하였다. 동물실험에서는 CT-26 세포를 1.0×10(5)개씩 Balb/c 생쥐의 피하에 접종하여 종양을 유도하였다. 종양이 유도된 Balb/c 생쥐에 YB-1 안티센스 올리고핵산염을 꼬리정맥이나 종양조직에 주사한 후 종양의 크기를 측정하여 종양억제 효과를 관찰하였다. 결과: YB-1 안티센스 올리고핵산염은 CT-26 세포에서는 50 nM 이상 농도에서 Chang liver와 HepG2 세포에서는 10 nM 이상 농도에서 세포주의 성장을 강하게 억제하였지만 정상 조직세포의 성장에는 아무런 영향을 미치지 않았다. 안티센스 올리고핵산염을 DOTAP에 담지한 경우 세포성장 억제에 미치는 효과가 안티센스 올리고핵산염 단독 처치 보다 강하였다. 이때 YB-1의 발현은 증식이 억제된 세포주(Chang liver 및 CT-26)에서는 감소하였으나 정상조직세포에서는 변화가 없었다. 증식이 억제된 세포주에서 세포주기를 살펴보면 초기에 S phase가 감소함을 관찰 할 수 있었다. 꼬리정맥이나 종양조직에 YB-1 안티센스 올리고핵산염을 주입 시 종양의 크기가 유의하게 감소하였다. 결론: 이상의 실험 결과 YB-1 안티센스 올리고핵산염은 암세포의 성장을 저해하며 종양 동물모델에서 종양의 성장을 억제할 수 있음을 시사하였다. Background: Human YB-1 is a transcription factor that binds to the inverted CCAAT box in the promoter region of a variety of genes such as PCNA, DNA polymerase and MDR. In this study we evaluated the effect of YB-1 antisense oligonucleotides on tumor cell growth. Methods: Chang liver, HepG2 and CT-26 cells were cultured as immortalized cell lines. The MTT (3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyltetrazolium bromide) assay, Northern blot and flow cytometric analyses were used to determine cell growth, gene expression and cell cycle changes. In an animal model, CT-26 cells were injected into Balb/c mice to induce tumor; YB-1 antisense oligonucleotides were injected into the tail vein or tumor tissue of the mice; change of tumor size was then measured. Results: Phosphorothioated YB-1 antisense oligonucleotides suppressed the proliferation of the immortalized liver cells (Chang liver cells) and a variety of cancer cells (HepG2 and CT-26 cells); however, it did not inhibit normal cell growth. The DOTAP/antisense oligonucleotide mixture showed stronger effects on cell proliferation than did the antisense oligonucleotide alone. The YB-1 antisense oligonucleotide decreased specific expression of the YB-1 mRNA in the immortalized cancer cell lines. Flow cytometric analysis revealed that the inhibition of cell proliferation might have been due to a decrease in the S phase of the cell cycle. We found that in an animal tumor model, the administration of the YB-1 antisense oligonucleotide, in the vein or tumor tissues, decreased the tumor size significantly. Conclusions: These results suggest that the YB-1 antisense oligonucleotide may inhibit growth of a variety of cancer cells.(Korean J Med 71:293-301, 2006)

土性에 따른 煙草植物의 養分吸收의 變化

陸昌洙,洪淳達,金雄柱,鄭勳采 충북대학교 엽연초연구소 1983 煙草硏究 Vol.10 No.-

Experiment was conducted to investigate the relation of growth characteristics, nutrient amount absorbed by tobacco plant, chemical components, changes of NH4 -N and NO3-N in the soil, yield, quality and price per 10a. Nc2326 and Burley 21-tobacco plant grew in sandy loam and clay loam by treated different levels of nitrogen, phosphorous and potassium fertilizers. the results were summarized as follows. 1.The plant height, length and width of the largest leaf, fresh weight and dry weight of tobacco leaves and stem of NC2326 and Burley21 were greater in clay loam than those in the sandy loam during growing. 2.The amount of nitrogen and phosphorous in tobacco leaves and stem absorbed by NC 2326 grown in clay loam were increased by 60days after transplanting, and then decreased, and amount absorbed by Burley 21 increased continuously in sandy loam and clay loam till the harvesting time. 3.The contents of potassium, calsium and magnesium in the leaves of NC 2326 and Burley 21 were increased continously during growing period and the amount of patassium uptake was greatest. 4.Total nitrogen, total alkaloid and reducing sugar in the dry leaves of NC 2326 and Burley 21 showed no differences between the plot of sandy loam and clay, but Burley 21 showed higher content of total nitrogen and alkaloid than NC2326. 5.Changes of NH4-N and NO3 -N in soil decreased gradually during growing period. Amount of NH4 -N in the plot of Burley 21 was higher than that in NC 2326 during glowing period. 6.The best yield was shown in the NC 2326 glowing in clay loam soil and quality was highest in the sandy loam. The highest price per 10a, 590, 400, was shown in clay loam with 10-10-20kg/10a fertilization.

Wiener 空間에서의 强한 極限의 定理에 對한 小考

張鑄燮 漢陽大學校 基礎科學硏究所 1981 基礎科學論文集 Vol.1 No.-

R.H. Cameron과 W.T. Martin(1947)이 "Wiener 空間에서의 强한 極限의 定理"를 (0,1)의 分割을 Diadic 數列에 對하여 보였고, K.S. Chang(1980)은 이 定理를 이 Diadic 數列을 품는 좀 더 큰 數列에 對하여서도 成立함을 보였다. 本 論文에서는 R.iH. Cameron과 W.T. Martin이 使用한 數列과 K.S, Chang이 使用한 數列을 좀더 一般化시켰고, 이로부터 위 두 數列은 勿論 포함하고 如前히 Wiener 空間에서의 强한 極限의 定理를 滿足하는 一般化된 數列의 形態를 얻었다.

Bunker C 유로 오염된 해사에서 bioremediation 에 의한 미생물군집의 변화

권창주,송홍규 ( Chang Joo Kwon,Hong Gyu Song ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.2

The changes of microbial numbers and activities during the bioremediation of beachsand contaminated with recalcitrant Bunker C fuel oil were investigated using microcosms. The numbers of total hydrocarbon degrading bacteria were rapidly increased and reached to 107cells/g sand, which was 10^4-10^5 times of increase. The numbers of aromatic hydrocarbon degraders showed similar trends but 1/10 less numbers. The aerobic heterotrophs jumped from 10^6cells/g to 10^9cells/g during 2 weeks of incubation. FDA direct count which stained only metabolically active bacteria also showed similar level and changes. The FDA hydrolysis activity was doubled by bioaugmentation and dehydrogenase activity was increased at 5∼6 times than control samples. Although the microbial numbers and activities in contaminated beachsand were not coincided exactly with the removal pattern of Bunker C fuel oil, this study showed the positive effects of bioaugmentation of microbial consortium.

흰쥐에서 분리 배양한 척수앞뿔신경세포의 형태 분화 및 축삭 형성

엄창섭,엄창섭,박창현,권계숙,유임주,박선화,전용혁 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.2

Changes of soil organic carbon and its fractions in relation to soil physical properties in a long-term fertilized paddy

Lee, Seul Bi,Lee, Chang Hoon,Jung, Ki Yuol,Park, Ki Do,Lee, Dokyoung,Kim, Pil Joo Elsevier 2009 Soil & tillage research Vol.104 No.2

<P><B>Abstract</B></P><P>Soil organic carbon (SOC) has an important role in improving soil quality and sustainable production. A long-term fertilization study was conducted to investigate changes in SOC and its relation to soil physical properties in a rice paddy soil. The paddy soils analyzed were subjected to different fertilization practices: continuous application of inorganic fertilizers (NPK, N–P–K=120–34.9–66.7kgha<SUP>−1</SUP>yr<SUP>−1</SUP> during 1967–1972 and 150–43.7–83.3kgha<SUP>−1</SUP>yr<SUP>−1</SUP> from 1973 to 2007), straw based compost (Compost, 10Mgha<SUP>−1</SUP>yr<SUP>−1</SUP>), a combination of NPK+Compost, and no fertilization (control). Soil physical properties were investigated at rice harvesting stage in the 41st year for analyzing the relationship with SOC fraction. Continuous compost application increased the total SOC concentration in plough layers and improved soil physical properties. In contrast, inorganic or no fertilization markedly decreased SOC concentration resulting to a deterioration of soil physical health. Most of the SOC was the organo-mineral fraction (<0.053mm size), accounting for over 70% of total SOC. Macro-aggregate SOC fraction (2–0.25mm size), which is used as an indicator of soil quality rather than total SOC, covered 8–17% of total SOC. These two SOC fractions accumulated with the same tendency as the total SOC changes. Comparatively, micro-aggregate SOC (0.25–0.053mm size), which has high correlation with physical properties, significantly decreased with time, irrespective of the inorganic fertilizers or compost application, but the mechanism of decrease is not clear. Conclusively, compost increased total SOC content and effective SOC fraction, thereby improving soil physical properties and sustaining production.</P>