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Establishment of a Resource Population of SLA Haplotype-Defined Korean Native Pigs
Cho, Han-Ok,Ho, Chak-Sum,Lee, Yu-Joo,Cho, In-Cheol,Lee, Sung-Soo,Ko, Moon-Suck,Park, Chan-Kyu,Smith, Douglas M.,Jeon, Jin-Tae,Lee, Jun-Heon Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.5
The highly polymorphic porcine major histocompatibility complex (MHC), or the swine leukocyte antigens (SLA), has been repeatedly associated with variations in swine immune response to pathogens and vaccines as well as with production traits. The SLA antigens are also important targets for immunological recognition of foreign tissue grafts. We recently established a resource population of Korean native pigs as models for human transplantation and xenotransplantation research. In this study, 115 animals derived from three generations of the Korean native pigs were genotyped for three SLA class I (SLA-2, SLA-3 and SLA-1) and three SLA class II loci (DRB1, DQB1, DQA) using PCR with sequence-specific primers (PCR-SSP) at the allele group resolution. A total of seven SLA haplotypes (Lr-5.34, Lr-7.23, Lr-31.13, Lr-56.23, Lr-56.30, Lr-59.1, Lr-65.34), comprising six unique class I and five unique class II haplotypes, were characterized in the founding animals. Class I haplotype Lr-65.0 and class II haplotype Lr-0.34 were novel; and together with Lr-56.0 these haplotypes appeared to be breed-specific. In the progeny population, Lr-7.23 and Lr-56.30 appeared to be the most prevalent haplotypes with frequencies of 34.7% and 31.6%, respectively; the overall homozygosity was 27.4%. This resource population of SLA-defined Korean native pigs will be useful as large animal models for various transplantation and xenotransplantation experiments, as well as for dissecting the roles of SLA proteins in swine disease resistance and production traits.
Establishment of a Resource Population of SLA Haplotype-Defined Korean Native Pigs
Han-Ok Cho,Chak-Sum Ho,Yu-Joo Lee,In-Cheol Cho,Sung-Soo Lee,Moon-Suck Ko,박찬규,Douglas M. Smith,전진태,이준헌 한국분자세포생물학회 2010 Molecules and cells Vol.29 No.5
The highly polymorphic porcine major histocompatibility complex (MHC), or the swine leukocyte antigens (SLA), has been repeatedly associated with variations in swine immune response to pathogens and vaccines as well as with production traits. The SLA antigens are also impor-tant targets for immunological recognition of foreign tis-sue grafts. We recently established a resource population of Korean native pigs as models for human transplantation and xenotransplantation research. In this study, 115 ani-mals derived from three generations of the Korean native pigs were genotyped for three SLA class I (SLA-2, SLA-3 and SLA-1) and three SLA class II loci (DRB1, DQB1, DQA) using PCR with sequence-specific primers (PCR-SSP) at the allele group resolution. A total of seven SLA haplo-types (Lr-5.34, Lr-7.23, Lr-31.13, Lr-56.23, Lr-56.30, Lr-59.1, Lr-65.34), comprising six unique class I and five unique class II haplotypes, were characterized in the founding animals. Class I haplotype Lr-65.0 and class II haplotype Lr-0.34 were novel; and together with Lr-56.0 these haplo-types appeared to be breed-specific. In the progeny popu-lation, Lr-7.23 and Lr-56.30 appeared to be the most preva-lent haplotypes with frequencies of 34.7% and 31.6%, re-spectively; the overall homozygosity was 27.4%. This re-source population of SLA-defined Korean native pigs will be useful as large animal models for various transplanta-tion and xenotransplantation experiments, as well as for dissecting the roles of SLA proteins in swine disease re-sistance and production traits.