E1A physically interacts with RUNX3 and inhibits its transactivation activity

Cha, Eun‐,Jeong,Oh, Byung‐,Chul,Wee, Hee‐,Jun,Chi, Xin‐,Zi,Goh, Yun‐,Mi,Lee, Kyeong‐,Sook,Ito, Yoshiaki,Bae, Suk‐,Chul Wiley Subscription Services, Inc., A Wiley Company 2008 Journal of cellular biochemistry Vol.105 No.1

<P><B>Abstract</B></P><P>The adenoviral gene, termed <I>early region 1A</I> (<I>E1A</I>), is crucial for transformation and has been used very effectively as a tool to determine the molecular mechanisms that underlie the basis of cellular transformation. pRb, p107, p130, p300/CBP, p400, TRRAP, and CtBP were identified to be E1A‐binding proteins and their roles in cellular transformation have been established. Although the major function of E1A is considered to be the regulation of gene expression that is critical for differentiation and cell cycle exit, one of the most significant questions relating to E1A transformation is how E1A mediates this regulation. RUNX3 is a transcription factor that was first described as a gastric cancer tumor suppressor but is now known to be involved in many different cancers. Exogenous expression of <I>RUNX3</I> strongly inhibits the growth of cells. Here, we show that the adenovirus oncoprotein E1A interacts with RUNX3 in vitro and in vivo. RUNX3 interacts with the N‐terminus (amino acids 2‐29) of E1A, which is known to interact with p300/CBP, p400, and TRRAP. E1A interacts directly with the Runt domain of RUNX3 but does not interfere with CBFβ‐RUNX3 interactions. In addition, E1A inhibits the transactivation activity of RUNX3 on the <I>p21</I><SUP><I>WAF1/CIP1</I></SUP> promoter. Consistent with these observations, the growth inhibition induced by RUNX3 is reduced by E1A. These results demonstrate that E1A specifically binds to RUNX3 and inactivates its transactivation activity. We propose that one of the mechanisms for the oncogenic activity of E1A is the inhibition of RUNX3, similar to that of RB and p300/CBP. J. Cell. Biochem. 105: 236–244, 2008. © 2008 Wiley‐Liss, Inc.</P>

The correlation between tissue specific functional genes and cytokine signaling in liver

Chaeun Oh,Su-hyang Han,Sunyoung Jang,Kyung Hyun Yoo 한국실험동물학회 2019 한국실험동물학회 학술발표대회 논문집 Vol.2019 No.7

Identification of cytokine-regulated tissue specific STAT5 target genes

Chaeun Oh,Eunchae Park,Sungju Jung,Su Min Yoon,Su-hyang Han,Sunyoung Jang,Sumin Oh,Kyung Hyun Yoo 한국실험동물학회 2018 한국실험동물학회 학술발표대회 논문집 Vol.2018 No.7

지식기반사회에서 무용 인적자원의 역량 증진 전략

차은주(ChaEun-joo),안병순(AhnByoung-soon) 한국체육학회 2017 한국체육학회지 Vol.56 No.3

본 연구의 목적은 지식기반사회에서 무용 인적자원의 역량 강화를 위한 전략을 제시하는 것이다. 이를 위해 문헌고찰을 실시하여 지식기반사회의 특성을 파악하고 쟁점으로 대두되는 역량개발의 개념을 살펴보았으며, 무용역량 관련 현황 및 특성을 분석하고 무용 인적자원의 역량 강화를 위한 전략을 제시하고자 하였다. 그 결과 첫째, 지식기반사회는 지식이 가치 창출의 원동력이 되는 사회로, 각 분야에서 인재를 양성하기 위한 전략으로 개인의 역량개발이 중요한 과제로 대두되고 있으며 이는 직무와 교육 분야를 중심으로 진행되고 있다. 둘째, 무용분야에서 역량관련 연구가 진행되고 있으나 무용역량의 개발 방향에 대한 논의가 부족한 실정으로 구체적인 전략이 필요한 것으로 나타났다. 셋째, 무용 인적자원의 역량을 개발하고 강화하기 위한 전략으로는 무용역량의 중요성 인식 및 역량평가가 필요하며, 우수한 인적자원 확보, 무용 인적자원 개발을 위한 역량기반 무용 교육과정의 변화와 무용직무분야 분석을 통한 대학교과과정 연계, 무용전공자의 역량 강화를 위한 프로그램 개발이 필요하다. 이는 지식기반사회에서 무용분야가 경쟁력을 갖추기 위해 무용 인적자원의 역량 강화에 필요한 실천적 노력의 방향성을 제시하는데 의의가 있다. The purpose of this study is to suggest a strategy for enhancing competences of dance human resources in knowledge-based society. For achieving this purpose, based on literature review, the characteristics of knowledgebased society and the concept and the concept of competence development, which is becoming an issue today, were examined. Also, the present state and the characteristics of dance competence were analyzed and a strategy for enhancing competences of dance human resources was suggested. The study findings are as follows. First, in knowledge-based society, knowledge is the driving force of value creation and individual competence development is becoming an important task as a strategy to cultivate talent in different fields, specially in the field of job and education. Second, there are competence-related studies in the field of dance, but there is a shortage of discussion on the direction of dance competence development and systematic strategies are required. Third, there is a need to recognize and evaluate the importance of dance competence as a strategy to develop and strengthen dance human resources. It is required to securing superior human capital, change the competence-based dance curriculum for development of dance human resources, connect with the college curriculum by analyzing the field of dance-related job and develop a program for enhancing competences of dance majors. This study has significance by presenting the orientation of practical effort, which is required for enhancing competences of dance human resources. Ultimately, this would help the field of dance obtain competitiveness in knowledge-based society.

학생선수 핵심역량 진단 검사지 개발

차은주(ChaEun-joo),손환(SonHwan) 한국체육학회 2017 한국체육학회지 Vol.56 No.2

이 연구의 목적은 학생선수 핵심역량 진단 검사지 개발을 위한 것이다. 이를 위해 학생선수 핵심역량 관련 선행연구를 토대로 요인 구조를 설정하고 초기문항을 개발하였다. 개발된 초기문항은 전문가 및 중등학교 학생선수 14명을 대상으로 내용타당도 검증을 실시하여 예비문항을 선정하였다. 최종문항 개발을 위해 서울지역 중·고등학교 학생선수 626명을 대상으로 설문조사를 실시하였다. 그 중에서 322명을 대상으로 탐색적 요인분석을 실시하여 60문항을 선정하였으며, 그 외 304명을 대상으로 확인적 요인분석을 실시하여 타당성을 확보하였다. 최종 개발된 검사지는 6개 핵심역량, 17개 역량요인, 60개 문항으로 구성되었다. 구체적으로 정체성인식역량은 2요인(자기인식, 자기의지) 6문항, 자기관리역량은 4요인(목표설정, 시간관리, 체력관리, 스트레스관리) 13문항, 진로계발역량은 2요인(진로탐색, 진로의사결정) 9문항, 소통공감역량은 4요인(지도자, 가족, 친구, 사회) 16문항, 리더십역량은 2요인(조직특성인지, 팀워크) 7문항, 창의융합역량은 3요인(도구적상상력, 글로벌문화이해, IT매체활용) 9문항으로 나타났다. 이와 같은 절차를 통해 개발된 학생선수 핵심역량 진단 검사지는 중·고등학교 학생선수의 운동과 학업성취를 판단하는 준거로서 핵심역량 진단 및 강화를 지원하는 도구로 활용될 수 있을 것이다. This paper aims to develop the student athletes’core competencies assessment tool. To achieve this, based on precedent studies, this study identified the factor structure of student athletes’ core competencies and developed 102 initial questions through expert group meeting. It also conducted a content validity verification of the questions mentioned above in 14 experts and middle and high school student athletes and selected 85 preliminary questions. To draw the final questions, it conducted a survey of 626 student athletes at middle and high schools in Seoul. Among which, it conducted an exploratory factor analysis of 322 subjects who were assigned to wireless case selection and selected 60 questions. It also conducted a confirmatory factor analysis of the remaining 304 subjects and secured validity. It appeared that there were three personal domains and eight subfactors and that there were three social domains and nine subfactors. The Student Athletes’Core Competencies Assessment Tool developed through this procedure has secured its validity and is expected to be used as a tool to determine the achievement of middle and high school student athletes and support to improve their ability.

중계기 기반 다중 안테나 시스템에서 시공간 다이버시티를 얻을 수 있는 전송 시나리오 및 심볼 결합 기법

강채언(Chaeun Kang),이윤식(Woonsik Lee),정정한(Junghan Jung),이황수(Hwang Soo Lee) 한국통신학회 2005 한국통신학회 학술대회논문집 Vol.2005 No.11

무용 감성역량 모델 개발 연구

차은주(ChaEun-joo),안병순(AhnByoung-soon) 한국체육학회 2017 한국체육학회지 Vol.56 No.4

이 연구의 목적은 무용 조직(집단)의 기능 향상을 위해 무용 감성역량 모델을 개발하는 것이다. 이를 위해 감성역량 관련문헌고찰을 실시하여 무용 감성역량의 개념을 정립하고 무용 감성역량의 구성개념을 살펴보았다. 또한 전문가를 대상으로 포커스그룹 면담을 실시하여 무용 감성역량의 개념, 구성요인에 대한 내용타당도를 확보하였다. 그 결과 첫째, 무용 감성역량의 수준은 5단계로 기초수준, 실무·경험적 수준, 전문적 수준, 전문·응용 수준, 전문적 수준 그리고 지도 수준으로 구분되었다. 둘째, 무용 감성역량 구성요인은 개인영역과 조직영역의 2개 영역으로 인식과 조절의 차원으로 구분되었다. 인식차원은 감성인식, 감성이해로 구성되며, 조절차원은 감성조절, 감성활용으로 구분되었다. 이는 무용 감성역량의 개념과 구성요인을 이론적으로 제시함으로써 무용 조직(집단)의 리더와 구성원의 감성역량 특성을 파악하기 위한 기초자료로 활용되어 무용 감성역량 개발의 실천에 기여할 것으로 기대한다. The purpose of this study is to develop a dance emotional competence model for functional improvement of dance organizations(groups). In order to achieve this purpose, the concept of dance emotional competence was established, and the construct of a dance emotional competence model was considered, based on emotional competence-related literature. Also, a focus group interview with experts was conducted to verify the content validity of the concept and the components of dance emotional competence. The study findings are as follows. First, dance emotional competence was classified into five levels; a basic level, a practical and empirical level, a professional level, a professional and application level and a professional and guidance level. Second, the components of dance emotional competence were divided into recognition and regulation in two areas; a personal area and an organizational area. Recognition of a personal area was subdivided into emotional recognition and emotional understanding, and regulation was subdivided into emotional regulation and emotional application. Also, a organizational area was divided into recognition and regulation. It is considered that this study would serve as basic data to understand emotional competence characteristics of dance organization(group) leader and members and contribute to development of dance emotional competence by presenting the concept and the components of dance emotional competence in a theoretical way.

대학 무용전공자 핵심역량 지표 개발

차은주(ChaEun-Joo) 한국체육학회 2019 한국체육학회지 Vol.58 No.5

본 연구는 대학 무용전공자의 핵심역량 지표를 개발하는 것에 목적이 있다. 이를 위해 핵심역량 관련 문헌연구를 기반으로 대학무용전공자의 핵심역량 모델을 구성하고 진로개발의 성과가 우수한 21명의 무용전공자를 대상으로 심층면담을 통해 내용요소의 특성을 살펴보고 개념화하였다. 또한 무용 및 역량 관련 분야 전문가 6명을 대상으로 전문가협의회를 실시하여 구성체계의 타당성을 확보하였다. 그 결과 첫째, 대학 무용전공자의 핵심역량 지표 개발의 방향성은 인지역량을 기초로 학습역량과 직업역량을 개발하여 현재의 역량이 미래 역량으로 강화되는 것이 필요하다. 둘째, 대학 무용전공자 핵심역량 구성요소는 학습역량(학습인지, 학습자세, 학습실천, 학습관리)과 직업역량(진로의식, 직업탐색, 진로설계, 진로계발)으로 나타났다. 셋째, 무용전공자의 핵심역량 지표는 학습역량 요소의 경우 11개의 하위지표(학습관점, 자아인지, 자신감인지, 학습이유, 학습태도, 학습계획, 학습활동, 학점관리, 자기관리, 학습환경관리, 학습원칙)와 56개의 행동지표로 구성되었으며, 직업역량 요소는 8개의 하위지표(자기 이해, 직업세계, 직업정보탐색, 직장구조탐색, 진로목표, 직업설계, 직업준비, 직업실천)와 46개의 행동지표로 구성되었다. 이는 대학 무용전공자가 진로개발을 위해 자기주도적으로 핵심역량을 강화할 수 있는 규범적 기준을 제시하는데 의의가 있다. This study aims to develop core competency indicators of university dance majors. For this study, a core competency model of university dance majors was constructed based on literature reviews on core competencies and there was an in-depth interview with 21 excellent dance majors, who achieved results in career development to consider and conceptualize the characteristics of the contents. Then an experts’ meeting with 6 experts in dance and competencies was conducted to obtain the validity of the organization system. The study results are as follows. First, in terms of the orientation of core competency indicators of university dance majors, there is a need to develop learning competencies and vocational competencies based on cognitive competencies in order to enhance future competencies. Second, components of university dance majors’ core competencies were learning competencies (learning cognition, learning attitude, learning practice, learning management) and vocational competencies (career consciousness, career search, career design, career development). Third, with regard to core competency indicators of dance majors, components of learning competencies consist of 11 sub-indicators (learning perspective, self-cognition, confidence cognition, reason for learning, learning attitude, learning plan, learning activity, grade management, self-management, learning environment, learning principle) and 56 behavioral indicators, and components of vocational competencies consist of 8 sub-indicators (self-understanding, the occupational world, job information search, workplace structure search, career goal, job design, job preparation, job practice) and 46 behavioral i ndicators. In c onclusion, t his s tudy h as s ignificance b y proposing n ormative s tandards f or s elf-directed enhancement of core competencies, which are required for career development of university dance majors.

Functional roles of CTCF in breast cancer

( Sumin Oh ),( Chaeun Oh ),( Kyung Hyun Yoo ) 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.9

CTCF, Zinc-finger protein, has been identified as a multifunctional transcription factor that regulates gene expression through various mechanisms, including recruitment of other co-activators and binding to promoter regions of target genes. Furthermore, it has been proposed to be an insulator protein that contributes to the establishment of functional threedimensional chromatin structures. It can disrupt transcription through blocking the connection between an enhancer and a promoter. Previous studies revealed that the onset of various diseases, including breast cancer, could be attributed to the aberrant expression of CTCF itself or one or more of its target genes. In this review, we will describe molecular dysfunction involving CTCF that induces tumorigenesis and summarize the functional roles of CTCF in breast cancer. [BMB Reports 2017; 50(9): 445-453]

Tyrosinase inhibition and anti-melanin generation effect of cinnamamide analogues

Ullah, Sultan,Park, Chaeun,Ikram, Muhammad,Kang, Dongwan,Lee, Sanggwon,Yang, Jungho,Park, Yujin,Yoon, Sik,Chun, Pusoon,Moon, Hyung Ryong Academic Press 2019 Bioorganic chemistry Vol.87 No.-

<P><B>Abstract</B></P> <P>Abnormal melanogenesis results in excessive production of melanin, leading to pigmentation disorders. As a key and rate-limiting enzyme for melanogenesis, tyrosinase has been considered an important target for developing therapeutic agents of pigment disorders. Despite having an (<I>E</I>)-β-phenyl-α,β-unsaturated carbonyl scaffold, which plays an important role in the potent inhibition of tyrosinase activity, cinnamic acids have not attracted attention as potential tyrosinase inhibitors, due to their low tyrosinase inhibitory activity and relatively high hydrophilicity. Given that cinnamic acids’ structure intrinsically features this (<I>E</I>)-scaffold and following our experience that minute changes in the chemical structure can powerfully affect tyrosinase activity, twenty less hydrophilic cinnamamide derivatives were designed as potential tyrosinase inhibitors and synthesised using a Horner-Wadsworth-Emmons reaction. Four of these cinnmamides (<B>4</B>, <B>9</B>, <B>14</B>, and <B>19</B>) exhibited much stronger mushroom tyrosinase inhibition (over 90% inhibition) at 25 µM compared to kojic acid (20.57% inhibition); crucially, all four have a 2,4-dihydroxy group on the β-phenyl ring of the scaffold. A docking simulation using tyrosinase indicated that the four cinnamamides exceeded the binding affinity of kojic acid, and bound more strongly to the active site of tyrosinase. Based on the strength of their tyrosinase inhibition, these four cinnamamides were further evaluated in B16F10 melanoma cells. All four cinnamamides, without cytotoxicity, exhibited higher tyrosinase inhibitory activity (67.33 – 79.67% inhibition) at 25 μM than kojic acid (38.11% inhibition), with the following increasing inhibitory order: morpholino (<B>9</B>) = cyclopentylamino (<B>14</B>) < cyclohexylamino (<B>19</B>) < <I>N</I>-methylpiperazino (<B>4</B>) cinnamamides. Analysis of tyrosinase activity and melanin content in B16F10 cells showed that the four cinnamamides dose-dependently inhibited both cellular tyrosinase activity and melanin content and that their inhibitory activity at 25 μM was much better than that of kojic acid. The results of melanin content analysis well matched those of the cellular tyrosinase activity analysis, indicating that tyrosinase inhibition by the four cinnamamides is a major factor in the reduction of melanin production. These results imply that these four cinnamamides with a 2,4-dihydroxyphenyl group can act as excellent anti-melanogenic agents in the treatment of pigmentation disorders.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Twenty cinnamamide derivatives were synthesized as potential tyrosinase inhibitors. </LI> <LI> Four cinnmamides exhibited over 90% mushroom tyrosinase inhibitions than kojic acid. </LI> <LI> In B16F10 cells, four compounds strongly decreased tyrosinase activity and melanin. </LI> <LI> In docking study, four cinnamamides bind to tyrosinase strongerly than kojic acid. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>