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      • IBA處理가 감나무 初期生育에 미치는 影響

        蔡潤錫,朴相眩,安相烈 진주산업대학교 1993 論文集 Vol.32 No.-

        One year old seedings of persimmon trees treated with IBA, growth regulator, in different levels were subjected to test. After one year of cultivation, the effect of IBA on the plant height, the root length, and the average weight per leaf were determined. The treatment 500 and 1000 ppm of IBA trended positive result of growth.

      • 여름 고추육묘의 트라이졸계 처리에 의해 유기된 생육억제 해소에 관한 연구

        채윤석,강호종,황서진 진주산업대학교 농업기술연구소 2007 農業技術硏究所報 Vol.20 No.-

        고온기 고추육묘에 처리한 생장억제제 처리 효과 및 정식 후 생육억제를 회복하기 위하여 GA, 관수, 고온처리 효과에 대한 연구 결과는 다음과 같다. 1.생장억제제 트리아졸계 처리 횟수에 따라 초장 및 주경장은 뚜렷한 차이를 나타냈고 근장도 2회 처리를 함으로 1회 처리를 한 것보다 발달하지 않았다. 2. 왜화된 고추묘의 생육억제 해소에 미치는 영향은 초장 및 주경장의 생장에는 GA처리구가 뚜렷하였으나 다른 처리구는 비슷한 경향이었고 경경은 변화가 없었다. 3. 생육회복을 위한 처리별 생체 및 건물중은 정식 후 30일까지는 변화가 거의 없었으나 그 후 45일까지 급격히 증가하였는데 고온 및 관수처리구에서 뚜렷한 차이를 보였으며 45일 이후에는 모든 처리구에서 비슷한 경향을 보였다. 4. 정식 45일 경 처리구간 엽록소 함량은 GA처리구에서 가장 낮았으며 다른 처리구간에는 비슷했다. This study was carried out to find effects of restoration of hot pepper seedling dwarfed by the treatment of diniconazole by introducing GA₃, Irrigation and high temperature at knight and the results obtained are summarized as follows. The main results of this research are summarized as follows. 1. In the case of plant height and main stem hight treated with the first time and second time diniconazole showed the strongest effect than untreatment. The root length treated with diniconazole the first time effect gradually disappeared than the second time. 2. Sprays of GA₃100ppm to the dwarfed seedlings by diniconazole pretreatment were effective in counteracting the plant height and main stem height growth inhibition caused by diniconazole pretreatment. 3. The fresh and dry weight of pepper seedling were not significantly different untill 30days in planting, but were increased to 45days from 30days in planting. 4. The chlorophyll content were decreased more or less treatment of GA₃ 100ppm after 45days in planting.

      • 기관내 삽관시 Slow induction과 Fentanyl 정주가 심혈역학적 변화에 미치는 영향

        윤재천,채종한,조남수,정종달,김용일 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.2

        Tracheal intubation is accompanied by varying degrees of sympathetic stimulation as reflected by increases in blood pressure and heart rate. Several clinical trials to reduce these effects by deepening anesthesia, using pharmacologic agents such as alpha and beta blockers,calcium channel blockers, fentanyl and lidocaine have been reported. To evaluate the effects of slow induction and fentanyl on responses of cardiovascular system induced by endotracheal intubation, we administered thiopental sodium 5㎎/㎏ (group Ⅰ), slow induction with O_(2)-N_(2)O-halothane( group Ⅱ), fentanyl 3uμ/㎏ with thiopental sodium 4㎎/㎏(group Ⅲ) for induction of anesthesia, and measured systolic and diastolic pressures, and heart rates before induction, and at 1,2,3,4, and 5 minutes after intubation. 1) In group Ⅰ, intubation caused a significant increase in systolic pressure (10%) diastolic pressure (15%) and heart rate (28%) at 1 min. after intubation but each parameter returned to the normal level within 5 min. 2) In group Ⅱ, increases in systolic pressure (12%), diastolic pressure (22%) and heart rate (23%) at 1 min. after intubation but there were no significant differences compared to group Ⅰ. 3) In group Ⅲ, the increase in systolic and diastolic pressure induced by intubation was abolished by fentanyl and showed significant decrease compared to group Ⅱ, increase in heart rate (4%) at 1 min. after intubation but were of no significance, and showed significant decrease compared to group Ⅱ but not at 3 min., 4 min. after intubation. These results suggested that a low dose of fentanyl significantly blunts postintubation hypertenion and tachycardia when used as an adjunct to thiopental sodium.

      • FAF1의 도메인 특이적 항체 제조 및 검증

        이윤숙,채진희,김은희 충남대학교 생물공학연구소 2009 생물공학연구지 Vol.15 No.-

        Fas associated factor 1 (FAF1) is involved in dual signaling mechanisms, one via the Fas death inducing signaling complex (DISC) and the other via down regulation of NF-&'B. Previous study identified 74 kDa protein, FAF1, as a novel substrate for activated caspase-3 upon various kinds of stimuli (Fas, TNF, TRAIL, STS and VP16), and FAF1 was found in the extracts of apoptotic cells. Commercially available FAF1 antibodies recognized only C-terminus of FAF1 by Western blotting, but not able to recognize its N-terminus. In this study, mouse antibody targeting a.a, 212-290 of FAF1 was generated. GST-tagged human FAF1 212-290 was expressed in BL21 and used as an antigen to immunize mice. Anti-FAFl 212-290 immunoglobulins have been purified using affinity purification by FAF1 1-305 truncate protein. The results reveal that the antibody specifically recognized FAF1 in both Western blotting and immunofIuroscence experiments. They will serve as useful tools for further analysis of FAF1 N-terminus.

      • Cytokinin류의 경엽처리가 봄감자 동화물질 전류에 미치는 영향

        蔡潤錫,姜鎬宗 진주산업대학교 2001 論文集 Vol.40 No.-

        Effect of Cytokinins(Kinetin and Benzyl adenin :BA) on the yields and carbohydrats and nitrogen compounds in the different parts of the spring potatoes as Stadies. Both kinetin and BA caused sisnifican (P <0.05) increare in yields, (carbohydrats and nitrogen compounds in the plant) while no sisnificar increares were observed in treatments the average tubes weights per plant were increased incread in low concentration of kinetin treatment where are in hight concentration of BA. Starch conteints in leaf treated with kinetin and BA showed lower than that of the control, and in stem 1∼2% higher than that of the control the starch con in treat the control. The starch sugar contents in the tuber treated with kinetins and BA has no significan difference between the treatment and the control. The free sugar contents in the leaf, freated with kinetins showed me much significan differentes between the treatment and the control.

      • KCI등재

        靑少年의 詩敎育에 관한 調査硏究

        尹在根 한양대학교 한국학연구소 1988 韓國學論集 Vol.14 No.-

        Teenagers should grow in the sound and healthy living conditions and to be good men for their own life and for the native country . To procure such aims they need general education which is both emotional and intellectual. But our teenagers go to school almost without the emotional. Teenagers desire their lives full of emotion. Such desire lead them to read lylic poems. What is the lylic poetry of Korea for them. That question caused me to take the researcy of Korean lylic songs. And through the research I tried to collect data for the poetry anthology for boys and girls. The research continued almost for two years. It was accomplished by three phases. The first step was the selection of lylic works by poets and teachers; the second the selection of the first data with the participation of boys and girls; the third the verification by school boys and girls. This paper had the topic on the result from the field work about the above research. By the field work the following could be confirmed: (1) Our teenagers loves the Korean lylic poems. (2) Primary school boys and girls like thr poetic brightness in poems. (3) Middle school boys and girls like passionate love songs. (4) High school boys and girls like the symbolic love songs as well as the meditative lylics. And they despised the didactic approaches to poetry in their text books.

      • 저염 오징어젓갈의 숙성에 미치는 향신성분의 영향

        채수규,허윤행,양철영,유희중 서울보건대학 1996 서울보건대학 부설 한국보건과학연구소 논문집 Vol.3 No.1

        저염젓갈의 숙성에 미치는 향신성분의 영향을 조사하기 위하여 저염 오정어 젓갈로부터 숙성에 관여하는 주요세균을 분리하여 마늘, 고추, 생강 등의 향신성분의 항균성을 조사하고, 또한 7.5% 식염향유 저염 오징어 젓갈에 마늘즙 또는 고추가루 등을 가한 것과 가하지 않은 시제품을 제조하여 20℃에서 숙성시키면서 생균수와 이화학 성분의 경시적 변화를 비교 조사하여 다음과 같은 결과를 얻었다. 1. 저염 오징어젓갈의 숙성에 관여하는 주요 세균을 분리하여 형태학적 및 생리학적 특성을 조사한 결과 Staphylococcus, Bacillus, Micrococcus, Pseudomonas, Moraxella, Flavobacterium으로 屬레벨에서 동정되었다. 2. 마늘즙, 고추가루 추출물 및 생강 추출물 등의 향신성분을 이용하여 젓갈로부터 분리한 세균에 대해 발육 저지 상태를 조사한 결과 마늘즙의 경우는 마늘즙의 농도 20%로부터 그 이상의 농도에 의해 강한 항균작용을 나타내었으나 고추가루 추출물은 Bacillus균주만이 약간 저해작용을 나타내었고 생강 추출물의 경우는 젓갈 분리 세균에 대해 거의 저해작용이 관찰되지 않았다. 3. 7.5% 식염 함유 저염 젓갈의 경우 제조 직후의 생균수는 10^(5)/g 이었으며, 20℃에서 숙성시켰을 때 숙성 4일째에 이미 10^(7)/g에 달하였고 숙성 10일째에 10^(8)/g에 달해 부패하기 시작하였다. 3%로 마늘즙을 첨가한 저염 젓갈의 경우는 숙성 4일째에 10^(5)/g 정도였으며 숙성 10일째에도 10^(7)/g 정도로 control에 비하여 10^(1) 정도 낮은 수준이었으며 숙성 21일째에 이르러도 부패되지 않았다. 4. 7.5% 식염 함유 저염 젓갈의 경우 제조 직후의 pH는 6.50 정도이었으며 숙성 7일째까지는 pH 6.45 정도로 미미한 감소추세를 나타내다가 그 이후부터는 다시 증가되어 숙성 15일째부터는 pH 7 이상으로 부패되었다. 3%로 마늘즙을 첨가한 저염 젓갈은 숙성 10일째까지는 pH 6.37 정도로 서서히 감소추세를 나타내다가 그 이후부터 다시 서서히 증가되어 숙성 21일째에 pH 6.85 정도를 나타내었다. 5. 7.5% 식염 함유 젓갈의 경우 제조 직후의 아미노태 질소 함량 208㎎%이었으나 20℃에서 숙성시켰을 때 숙성 7일째 364㎎%, 숙성 14일째 597㎎%로 증가하였다. 3%로 마늘즙을 첨가한 경우는 숙성 7일째에 327㎎%, 숙성 14일째에 515㎎%로 증가하여 두 시료간의 아미노태 질소 함량의 차이는 숙성 7일째에 37㎎/100g, 숙성 14일째에 82㎎/100g으로 나타났다. 6. 7.5% 식염 함유 저염 젓갈의 경우 제조 직후의 VBN함량은 21.2㎎%이었으며, 20℃에서 숙성시켰을 때 숙성 10일째에 116.5㎎%, 숙성 14일째에 180.0㎎%를 나타내었고 TMA-N은 숙성 14일째에 41.3㎎%를 나타내어 부패가 진행되었다. 3%로 마늘즙을 첨가한 저염 젓갈은 숙성 14일째에 84.2㎎%, 숙성 21일째에 116.3㎎%를 나타내었고, TMA-N은 숙성 21일째에도 검출되지 않아 부패되지 않고 적정 숙성상태를 유지하고 있었다. To study on the effects of spice ingredients on the aging of low salted squid with 7.5% salt the garlic juice or the red pepper powder was added in the low salted squid. The changes in the chemical composition and viable cell counts of the low salted squid were investigated, individually, during the ripening process at 20℃. The major bacteria were isolated from the salted squid during the aging period and the physiological and biochemical properties of the isolates were investigated. The strains were identified at genus level as the Staphylococcus, Bacillus, Micrococcus, Pseudomonas, Moraxella and Flavobacterium, respectively. By the spice ingredients of garlic juice, red pepper extracts and ginger extracts, the inhibition of bacterial growth were investigated. Antimicrobial action was strong in the concentration of over 20% garlic juice. But only the strain of Bacillus was inhibited weakly by red pepper water extracts. The inhibition of bacterial growth by the ginger water extracts was not detected in the salted squid. Just after the preparation of the low salted squid with 7.5% salt, the viable bacterial cells occurred at a level of 10^(5)/g. The bacterial counts were increased to 10^(7)/g by the 4th day of ripening and increased to 10^(8)/g by the 10th day of ripening at 20℃ and then the salted squid was begun to spoil. In the low salted squid with 7.5% salt and 3% garlic juice, the bacterial counts were increased to 10^(5)/g by the 4th day of ripening and increased to 10^(7)/g by the 10th day of ripening at 20℃. The salted squid with 3% garlic juice was lower about 101 level than control in viable bacterial counts and it was not spoiled by the 21th day ripening at 20℃. Just after the preparation of the low salted squid with 7.5% salt, the pH value was 6.50. The pH value was decreased gradually to 6.45 by the 7th day of ripening at 20℃ and then the pH value was increased again to 7.0 by the 15th day of ripening and the salted squid was spoiled. In the low salted squid with 7.5% salt and 3% garlic juice the pH value was decreased gradually to 6.37 by the 10th day of was increased again to 6.85 by the 21th day of ripening. Just after the preparation of the low salted squid with 7.5% salt, the content of amino type nitrogen was 208mg%. The content of amino type nitrogen was increased to 364mg% by the 7th day of ripening and increased to 597mg% by the 14th day of ripening at 20℃. In the low salted squid with 7.5% salt and 3% garlic juice, the content of amino type nitrogen was increased to 327mg% by the 7th day of ripening and increased to 515mg% by the 14th day of ripening at 20℃. Therefore, the difference in amino type nitrogen content was 37mg/100g by the 7th day of ripening and 82mg/100g by the 14th day of ripening. Just after the preparation of the low salted squid with 7.5% salt, the contents of VBN was 21.2mg%. The content of VBN was increased to 116.4mg% by the 10th day of ripening and increased to 180.0mg% by the 14th day of ripening at 20℃. The content of TMA-N was 41.3mg% by the 14th day of ripening and the salted squid was begun to spoil. In the low salted squid with 7.5% salt and 3% garlic juice, the content of VBN was increased to 84.2mg% by the 14th day of ripening and increased to 116.3mg% by the 21th day of ripening at 20℃. The content of TMA-N was not detected by the 21th day of ripening. The salted squid was maintained on the proper ripening condition.

      • 葡萄 Muscat Bailey A의 無核果 生産을 爲한 Streptomycin 利用 試驗(Ⅱ)

        蔡潤錫,朴相眩,安相烈 진주산업대학교 농업기술연구소 1993 農業技術硏究所報 Vol.6 No.-

        GA_3 100ppm單用과 GA_3 100ppm+Streptomycim 200ppm 混用을 葡萄 Muscat Bailey A 品種의 滿開期 10日前에 新稍長 30, 40, 50cm의 着果房에 處理한 結果는 다음과 같다. 1. 熟期는 兩處理區 모두 無處理보다 20日 前後 早熟되었으며 混用區는 GA_3 單用區 보다 2∼3日 빠른 傾向을 보였고, 新稍의 길이에 따른 差異는 비슷하였다. 2. 有核粒數는 無處理 平均 5∼6個에 비해 混用 및 GA_3 單用 모두 無核의 傾向을 보였는데 新稍長 50cm의 GA_3 單用區에서 1∼2個의 有核粒을 나타내었다. 3. 果房長의 兩主區의 新稍길이 30cm와 40cm 處理區는 비슷하였으며 상품성이 없었고, 50cm, 處理區에서는 높은 有意性을 보였다. 4. 果房重은 GA_3 單用區가 混用區보다 80∼90g정도 무거웠으며, 新稍길이 間에는 50cm處理區에서 가장 무거웠고 과방당 과립수와 평균과립중도 50cm 처리구에서 고도의 유의성을 나타내었다. 5. 糖度는 混用區가 單用區보다 높고, 新稍의 길이間에는 길수록 다소 높은 傾向을 보였다. GA_3 100ppm solution and GA_3 100ppm+streptomycin 200ppm solution are applied on the cluster of 30, 40, 50cm shoot length of Muscat Bailey A variety at 10 days before full bloom day. The results are as follows : 1. The maturing season was 20 days or so ealier in both exprimental plots than in the control plot. The GA_3+streptomycin solution applied grapes atured 2∼3 days ealier than the GA_3 solution applied grapes. Thd shoot length of application revealed little difference. 2. The GA_3+streptomycin plot and GA_3 plot produced no seed holding berry, but the control plot produced 5∼6 seed holding berries. GA_3 of 50cm shoot length plot produced 1∼2 seed holding berries. 3. The cluster length of 30, 40cm shoot length plot among both main plots were similar and the value of merchandise were not found. The 50cm shoot length plot among both main plots showed high significant difference. 4. The cluster weight of GA_3 plot was significantly 80∼90g heaviet than that of GA_3+streptomycin plot. The cluster weight of 50cm shoot length plot was the heaviest. The berry number per cluster and average berry weight of 50cm shoot length plot showed high significant differencd. 5. The sugar content of berry was significant larger in the GA_3+streptomycin plot than in the GA_3 plot. The longer shoot length was, the larger the sugar content of berry was.

      • 石榴나무의 揷木에 關한 硏究 : 1. IBA가 發根에 미치는 影響 1. Effects on the Rooting of IBA in Punicaceae Tree

        蔡潤錫,박상현,金治永 진주산업대학교 농업기술연구소 1991 農業技術硏究所報 Vol.4 No.-

        石榴나무의 栽培面積 急增에 따른 揷木繁殖의 大量 生産을 爲한 基礎資料를 얻기 위하여, 植物生長調節制인 IBA가 石榴나무의 發根에 미치는 影響에 關한 試驗結果는 다음과 같다. 1. 發根率은 IBA의 處理濃度間에 有意性이 인정되었으며, 1500ppm에서 70.33%로 가장 높고, 2000ppm 區, 對照區, 1000ppm 順으로 나타났다. 2. 新梢生長의 길이는 各 處理間에 有意性이 인정되었으며, 1000ppm 區에서 가장 길고, 2000ppm 區, 對照區, 1500ppm 區 順이었다. 3. 根部무게는 1000ppm 區가 13.02g으로 가장 무거웠고, 그 밖의 各 處理區間에는 비슷한 傾向을 보였다. 4. 根部直徑은 全 處理區에서 비슷한 傾向을 보였다. 5. 1500ppm 區가 他區에 비해 대체로 良苗 狀態였다. Recently the pomegranates are grown wide, making it necessary to produce the cutting massively. The study is to examine the effects fo IBA hormone upon the rooting of pomegranates. The results of experiments are as follows; 1. The rooting percentage showed significant difference between each groups of different IBA concentrations. The highest was 70.33% in the 1500ppm group. The 2000ppm group showed the next highest percentage followed by the control and 1000ppm groups. 2. The length of new shoots also showed significant difference. The 1000ppm IBA concentration group produced the longest shoots, followed by the 2000ppm, control, and 1500ppm group in order. 3. The root weight was heaviest in the 1000ppm group (13.02g). The weights of the other groups were similar to each other. 4. The root diameter was similar in each group. 5. The 1500ppm group showed the best results.

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