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Elaine Carvalho Minighin,Roberto Ce´sar Santos de Sousa,Ana Luiza Coeli Cruz Ramos,Lorena Thais Souza Dias,Ana Luiza Coeli Cruz Ramos,Lorena Thais Souza Dias,Renata Adriana Labanca,Raquel Linhares Bel 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.5
Phenylketonuria (PKU) is an autosomal recessive disease caused by variants in the gene that encodes phenylalanine hydroxylase (PAH), limiting the metabolism of phenylalanine (Phe). When PAH activity is absent or hindered, Phe is not converted to tyrosine, leading to an accumulation of Phe in the blood, which can cause serious neurological complications. Once PKU is diagnosed, treatment should be started immediately, and the basis for this is dietary restriction of foods with high levels of Phe, associated with the use of protein substitutes and intake of foods with low protein content. This restriction accompanies patients throughout their lives, making their diets unpalatable and monotonous, which represents a major challenge for health professionals and patients, considering that these factors favor food transgression. In this context, the objective of this work was to carry out an integrative review based on evidence regarding the intake of fruits and vegetables, by phenylketonurics, taking into account the greater or lesser tolerance to Phe. Since, some researchers have dedicated themselves to evaluating the biochemical effect of unrestricted consumption of fruits and vegetables at PKU, unifying the information in this regard. It was observed that the intake of vegetable protein by patients with PKU has shown to be promising since the studies indicate that the intake of these proteins does not present adverse effects to the metabolic control of the Phe.
Silencing the cleavage factor CFIm25 as a new strategy to control Entamoeba histolytica parasite
Juan David Ospina-Villa,Nancy Guillén,Ce´sar Lo´pez-Camarillo,Jacqueline Soto-Sanchez,Esther Ramirez-Moreno,Raul Garcia-Vazquez,Carlos A. Castañon-Sanchez,Abigail Betanzos,Laurence A.Marchat 한국미생물학회 2017 The journal of microbiology Vol.55 No.10
The 25 kDa subunit of the Clevage Factor Im (CFIm25) is an essential factor for messenger RNA polyadenylation in human cells. Therefore, here we investigated whether the homologous protein of Entamoeba histolytica, the protozoan responsible for human amoebiasis, might be considered as a biochemical target for parasite control. Trophozoites were cultured with bacterial double-stranded RNA molecules targeting the EhCFIm25 gene, and inhibition of mRNA and protein expression was confirmed by RT-PCR and Western blot assays, respectively. EhCFIm25 silencing was associated with a significant acceleration of cell proliferation and cell death. Moreover, trophozoites appeared as larger and multinucleated cells. These morphological changes were accompanied by a reduced mobility, and erythrophagocytosis was significantly diminished. Lastly, the knockdown of EhCFIm25 affected the poly(A) site selection in two reporter genes and revealed that EhCFIm25 stimulates the utilization of downstream poly(A) sites in E. histolytica mRNA. Overall, our data confirm that targeting the polyadenylation process represents an interesting strategy for controlling parasites, including E. histolytica. To our best knowledge, the present study is the first to have revealed the relevance of the cleavage factor CFIm25 as a biochemical target in parasites.
Surelys Ramos-Bell,Montserrat Caldero´n-Santoyo,Julio Ce´sar Barros-Castillo,Juan Arturo Ragazzo-Sa´nchez 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.10
In this study, the stability of a submicronemulsion to protect an extract obtained from sea grape fruit(Coccoloba uvifera L.) was evaluated. Extract characterizationby MS-HPLC revealed the presence of 3 anthocyanins(cyanidin 3-glucoside, malvidin 3-glucoside, anddelphinidin 3-glucoside), the content of total phenols was263.86 ± 1.86 mg gallic acid equivalent/100 g, with anantioxidant capacity determined by ABTS and DPPH of128.95 ± 1.00 and 26.18 ± 0.60 lg Trolox equivalents/mL, respectively. A submicron emulsion (0.424 lm) byUltrasound with monomodal distribution, stable over timeand low viscosity (1.94 mPa s) classified as a shear-thinningfluid was obtained. The thermogravimetric analysis(TGA) demonstrated the stability of the C. uvifera extractin the emulsion, which is thermostable (212 C). Theseemulsions can be added into a beverage as a nutraceutical,dried for later use as pills or incorporated in foods.
Transcriptional profile of processing machinery of 30 end of mRNA in Trichomonas vaginalis
Miguel A ´ ngel Del-Moral-Stevenel,Alma Villalobos-Osnaya,Mavil Lo´pez-Casamichana,Laura Itzel Quintas-Granados,Ce´sar Lo´pez-Camarillo,Jose´ Manuel Ferna´ndez Sa´nchez,Selene Zarate-Guerra,Marı´a Eli 한국유전학회 2015 Genes & Genomics Vol.37 No.4
Trichomonas vaginalis is the causative agent of trichomonosis, a sexually transmitted disease (STD) that affects over 180 million people worldwide. This parasite is capable to infect the urogenital tract of women and men, both microenvironments might affect the expression of key genes that may be involved in the parasite pathogenesis. The processing of 30 end of mRNA promotes mRNA stability in many eukaryotes, however in T. vaginalis this molecular machinery is under research. By means of an in silico analysis we identified putative proteins of the 30 end mRNA processing machinery of T. vaginalis, and by RTPCR assays we evaluated the expression of eight of these genes in a female and male T. vaginalis isolates. According to the in silico analysis, the T. vaginalis 30 end mRNA processing machinery, comprises a similar complex and protein factors that those described in Homo sapiens, Arabidopsis thaliana, Saccharomyces cerevisiae and Entamoeba histolytica. The complex contains several subcomplexes, including cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), cleavage factor I (CFIm) and cleavage factor II (CFIIm). We demonstrated that genes tvpsf2p, tvcfi25, tvcpsf160, tvcpsf73, tvfip1, tvpap1, tvpc4 and tvpabp are expressed in male or female T. vaginalis isolates. Besides we identify two different isoforms of TvPC4. T. vaginalis genome contains most of genes encoding for 30 end mRNA processing, which may be transcriptionally active and could be involved in the capping, splicing, cleavage and polyadenylation of mRNAs in this parasite. Further studies are necessary to elucidate the biological meaning of our findings.
Maricelma da Silva Soares de Souza,Sandra Maria Barbalho,De´bora Cristina Damasceno,Marilza Vieira Cunha Rudge,Kleber Eduardo de Campos,Ana Carolina Goyos Madi,Bruno Ribeiro Coelho,Rodrigo Ce´sar Oliv 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.1
The aim of this study was to evaluate the effects of Passiflora edulis f. flavicarpa Degener (yellow passion) juice on the lipid profile and oxidative stress status of Wistar rats. Adult male Wistar rats were divided in two groups (n = 8 animals per group): the control group, which received water, and the treated group, which was given P. edulis juice (1,000 mg/kg). Both groups received by gavage treatment twice a day for 28 days. The treated group showed an increased high-density lipoproteincholesterol level and decreased low-density lipoprotein-cholesterol and free fatty acid levels compared with the control group. Levels of triglycerides and and very low-density lipoprotein-cholesterol, superoxide dismutase activity, and total glutathione concentration were not statistically different between the two groups, but the thiobarbituric acid–reactive substances concentration (indicating lipid peroxidation) decreased in the treated group. These findings suggests that P. edulis juice in the experimental conditions used showed beneficial effects on lipid profile and improved lipid peroxidation in Wistar rats.