Assessing the Metabolic Impact of Ground Chia Seed in Overweight and Obese Prepubescent Children: Results of a Double-Blind Randomized Clinical Trial

Camila Saran da Silva,Camila Ribeiro de Arruda Monteiro,Guilherme Henrique Fagundes da Silva,Roseli Oselka Sacardo Sarni,Fabiola Isabel Suano Souza,David Feder,Marcia Cristina Fernandes Messias,Patrıc 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.3

Childhood obesity is a medical condition of major public health concern. Chia seeds are used to treat certain noncommunicable diseases, and they are rich in omega-3 fatty acids, which contribute to the absorption of vitamins. A randomized double-blind clinical trial of 30 obese children was performed. The sample was composed of prepubertal 5- to 10-year-old children of both sexes with body mass indexes equal to or above the 95th percentile who were recruited through the Pediatric Department of the Faculdade de Medicina do ABC. Blood samples were drawn, the children were weighed and measured, and a 24-h dietary recall was obtained before and after the treatment. Not only were significant differences observed for fibrinogen (P = .011) but a correlation between the changes in markers and the presence of fibers was also observed for two inflammatory parameters: tumor necrosis factor-α (P = .027) and nuclear factor-κβ (P = .059). These results indicate that chia seeds may have anti-inflammatory effects related to their fiber content in the context of childhood obesity.

Effects of Lidocaine Injection at Acupuncture Points on Perioperative Analgesia in Cats Undergoing Ovariohysterectomy

Camila Menossi Sueza Lima,Camila Zanetti Segatto,Gustavo Ricci Zanelli,Gabriel Montoro Nicácio,Renata Navarro Cassu 사단법인약침학회 2022 Journal of Acupuncture & Meridian Studies Vol.15 No.4

Background: Pharmacopuncture is an acupuncture-related technique that has been used to amplify the therapeutic effects of different medications. Objectives: To investigate the analgesic efficacy of a lidocaine injection at acupoints in cats undergoing ovariohysterectomy. Methods: Thirty cats were randomly distributed into two groups (n = 15, per group). The experimental group received a bilateral administration of lidocaine at the following acupoints: Stomach 36 (ST-36) and Spleen 6 (SP-6) (Lido group). The control group did not receive lidocaine (Control group). All cats were sedated with dexmedetomidine and anesthesia was induced with propofol and maintained with isoflurane. Intraoperatively, fentanyl was given to control cardiovascular responses to surgical stimulation. Postoperative pain was assessed at various time points, up to 24 hours after extubation, using the UNESP-Botucatu multidimensional composite pain scale (MCPS) and Glasgow feline composite measure pain scale (CMPS-Feline). Sedation scores were measured at the same time points. Morphine/meloxicam was administered as rescue analgesia. Data were analyzed using t-tests, Fisher´s exact test, the Mann-Whitney test, and the Friedman test (p < 0.05). Results: Intraoperatively, more cats in the Control group required analgesic supplementation than those in the Lido group, but the difference was not significant (p = 0.65). Postoperative pain, sedation scores, and analgesic requirements did not differ between groups. Rescue analgesia was given to 67% (10/15) of the cats in each group. Conclusion: The administration of lidocaine at ST-36 and SP-6 acupuncture points did not provide significant perioperative analgesic benefits in healthy cats undergoing ovariohysterectomy.

Restoration and conservation of anatomic pieces

Camila Cárdenas Guerrero Guzmán,Karen Alejandra Pérez Díaz,María Paula Ruíz Díaz,Valentina Díaz Sánchez,Andrés Camilo Ariza Aguirre,Laura Catalina Cantor Alfonso,Camila Andrea Suárez Ortiz,Davide Fali 대한해부학회 2019 Anatomy & Cell Biology Vol.52 No.3

In this study, a restoration process was developed with potassium hydroxide (KOH), in order to improve each of the structures for their posterior fixation, through the use of new methods such as the Chilean conservative fixative solution (SFCCh), with exceptional results. Restore anatomical pieces corresponding to corpse and organs, being these last set with the SFCCh. In this work dealt with processes of restoration with potassium hydroxide, sodium chloride, and sodium hypochlorite, the process began with the cleanliness and suture of the structures for subsequent fixing in Chilean conservative fixative solution, making use of a corpse and different anatomical parts. Work based on items found in the database, Elsevier, Science Direct, ProQuest, and MEDLINE. At the end of the process of restoration and conservation of the anatomical pieces, was observed an improvement in muscle pigment with decrease of rigidity in the specimen, additionally a recovery of appearance in the vascular-nervous elements was achieved. The organs were much more malleable and the structures facilitate the identification of specific details, its subsequent immersion in SFCCh allows the longer preservation of the obtained results. The restoration with potassium hydroxide allows the improvement in the appearance of the different anatomical structures and simultaneously to facilitate its study. The SFCCh is an alternative that replaces partially the use of formaldehyde. In addition, it presents toxicity reduction.

Comparison of Climacteric Symptoms, Quality of Life, and Self-Care Attitudes before and during the COVID-19 Pandemic

( Camila Oliveira Serra ),( Paula Mara Gomes Leite ),( Andráa Beatriz Bezerra ),( Laura Freitas ),( Lucas Veras ),( Marcela Deda Costa ),( Leila Luiza Conceição Gonçalves ),( Leonardo Yung Dos Santos 대한폐경학회 2022 대한폐경학회지 Vol.28 No.1

Objectives: This study aimed to compare the climacteric symptoms, quality of life indices, and self-care attitudes in women before and during the coronavirus disease 2019 (COVID-19) pandemic. Methods: This cross-sectional study was conducted between January 2020 and September 2021. The sample consisted of 342 climacteric women who were divided into two groups: before the pandemic (BP group; n = 62) and during the pandemic (DP group; n = 280). The Menopause Rating Scale and Women’s Health Questionnaire were used to measure the health-related quality of life and degree of climacteric symptoms reported by women. Results: During the COVID-19 pandemic, women were able to decrease their somatic symptoms derived from the climacteric period (BP group: 7.84 ± 4.46, DP group: 5.94 ± 9.20; P = 0.003). Conclusions: There was no worsening in the self-reported symptoms, quality of life, and self-care attitudes of climacteric women because of the COVID-19 pandemic. Moreover, only somatic symptoms decreased during the pandemic.

Importance of Heat Shock Proteins in Maize

Camila Pegoraro,Antonio Costa de Oliveira,Liliane Marcia Mertz,Luciano Carlos da Maia,Cesar Valmor Rombaldi 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.2

Abiotic and biotic stress conditions cause extensive losses to maize production, mainly due to protein dysfunction in these conditions. In higher plants, the occurrence of heat-shock proteins (HSPs) in response to different environmental stresses is a universal phenomenon and has been well documented. Many studies have demonstrated that most HSPs are involved in many regulatory pathways,act as molecular chaperones for other cell proteins, and have strong cytoprotective effects. Although many functional roles for HSPs are known, the mechanisms for these multiple functions are not entirely understood. Here we reviewed the correlation among HSP genes/proteins and plant tolerance, especially maize, in different environmental stresses. Due to the low availability of information regarding the expression of HSP genes in response to different stresses in maize, we decided to mine databases in order to generate new insights related to this topic.

Restoration and conservation of anatomic pieces

Camila Cá,rdenas Guerrero Guzmá,n,Karen Alejandra Pé,rez Dí,az,Marí,a Paula Ruí,z Dí,az,Valentina Dí,az Sá,nchez,André,s Camilo Ariza Aguirre,Laura Catalina Can 대한해부학회 2019 Anatomy & Cell Biology Vol.52 No.3

In this study, a restoration process was developed with potassium hydroxide (KOH), in order to improve each of the structures for their posterior fixation, through the use of new methods such as the Chilean conservative fixative solution (SFCCh), with exceptional results. Restore anatomical pieces corresponding to corpse and organs, being these last set with the SFCCh. In this work dealt with processes of restoration with potassium hydroxide, sodium chloride, and sodium hypochlorite, the process began with the cleanliness and suture of the structures for subsequent fixing in Chilean conservative fixative solution, making use of a corpse and different anatomical parts. Work based on items found in the database, Elsevier, Science Direct, ProQuest, and MEDLINE. At the end of the process of restoration and conservation of the anatomical pieces, was observed an improvement in muscle pigment with decrease of rigidity in the specimen, additionally a recovery of appearance in the vascular-nervous elements was achieved. The organs were much more malleable and the structures facilitate the identification of specific details, its subsequent immersion in SFCCh allows the longer preservation of the obtained results. The restoration with potassium hydroxide allows the improvement in the appearance of the different anatomical structures and simultaneously to facilitate its study. The SFCCh is an alternative that replaces partially the use of formaldehyde. In addition, it presents toxicity reduction.

Comparative Metabolic Analysis of Lactate for CHO Cells in Glucose and Galactose

Camila A. Wilkens,Claudia Altamirano,Ziomara P. Gerdtzen 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.4

t-PA producing CHO cells have been shown to undergo a metabolic shift when the culture medium is supplemented with a mixture of glucose and galactose. This metabolic change is characterized by the reincorporation of lactate and its use as an additional carbon source. The aim of this work is to understand lactate metabolism. To do so,Chinese hamster ovary cells were grown in batch cultures in four different conditions consisting in different combinations of glucose and galactose. In experiments supplemented with glucose, only lactate production was observed. Cultures with glucose and galactose consumed glucose first and produced lactate at the same time, after glucose depletion galactose consumption began and lactate uptake was observed. Comparison of the metabolic state of cells with and without the shift by metabolic flux analysis show that the metabolic fluxes distribution changes mostly in the reactions involving pyruvate metabolism. When not enough pyruvate is being produced for cells to support their energy requirements, lactate dehydrogenase complex changes the direction of the reaction yielding pyruvate to feed the TCA cycle. The slow change from high fluxes during glucose consumption to low fluxes in galactose consumption generates intracellular conditions that allow the influx of lactate. Lactate consumption is possible in cell cultures supplemented with glucose and galactose due to the low rates at which galactose is consumed. Evidence suggests that an excessive production and accumulation of pyruvate during glucose consumption leads to lactate production and accumulation inside the cell. Other internal conditions such as a decrease in internal pH, forces the flow of lactate outside the cell. After metabolic shift the intracellular pool of pyruvate, lactate and H+ drops permitting the reversal of the monocarboxylate transporter direction, therefore leading to lactate uptake. Metabolic analysis comparing glucose and galactose consumption indicates that after metabolic shift not enough pyruvate is produced to supply energy metabolism and lactate is used for pyruvate synthesis. In addition, MFA indicates that most carbon consumed during low carbon flux is directed towards maintaining energy metabolism.

Differential Expression of Genes Associated with Endomembrane Transport and Cell Wall Metabolism during Ripening of ‘Chimarrita’ and ‘Granada’ Peach

Camila Pegoraro,Roberta Manica-Berto,F?bio Clasen Chaves,Carolina Terra Borges,Jader Job Franco,Cesar Valmor Rombaldi,Jorge Adolfo Silva 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.3

Expression of genes involved in endomembrane transport and cell wall metabolism, particularly in synthesis and degradation of cell wall macromolecules were compared during the ripening of a white melting flesh cultivar, Chimarrita and a yellow non-melting flesh cultivar, Granada in order to evaluate their association with fruit softening. In both, most genes were up-regulated as ripening evolved. In ‘Chimarrita’, overall higher transcript accumulation occurred at 95 days after anthesis, while in ‘Granada’ higher relative transcription was detected at 115 days after anthesis. Although transcript accumulation was correlated with fruit softening, the process does not seem to be totally dependent on the set of studied genes, since the peak of RNA abundance occurred when flesh firmness had already decreased.

Bioavailability of plant pigment phytochemicals in Angelica keiskei in older adults

Camila R Correa,C-Y. Oliver Chen,Giancarlo Aldini,Helen Rasmussen,Carlos F Ronchi,Carolina Berchieri-Ronchi,Soo-Muk Cho,Jeffrey B Blumberg,Kyung-Jin Yeum 한국영양학회 2014 Nutrition Research and Practice Vol.8 No.5

BACKGROUND/OBJECTIVES: Angelica keiskei is a green leafy vegetable rich in plant pigment phytochemicals such as flavonoids and carotenoids. This study examined bioavailability of flavonoids and carotenoids in Angelica keiskei and the alteration of the antioxidant performance in vivo. SUBJECTS AND MATERIALS: Absorption kinetics of phytochemicals in Angelica keiskei were determined in healthy older adults (> 60 y, n = 5) and subjects with metabolic syndrome (n = 5). Subjects consumed 5 g dry Angelica keiskei powder encapsulated in gelatin capsules with a low flavonoid and carotenoid liquid meal. Plasma samples were collected at baseline, 0.5, 1, 2, 3, 4, 5, 6, 7, and 8 h. Samples were analyzed for flavonoids and carotenoids using HPLC systems with electrochemical and UV detection, respectively, and for total antioxidant performance by fluorometry. RESULTS: After ingestion of Angelica keiskei increases in plasma quercetin concentrations were observed at 1-3 and 6-8 hr in the healthy group and at all time points in the metabolic syndrome group compared to baseline (P < 0.05). Plasma lutein concentrations were significantly elevated in both the healthy and metabolic syndrome groups at 8 hr (P < 0.05). Significant increases in total antioxidant performance were also observed in both the healthy and the metabolic syndrome groups compared to baseline (P < 0.05). CONCLUSIONS: Findings of this study clearly demonstrate the bioavailability of phytonutrients of Angelica keiskei and their ability to increase antioxidant status in humans.

A Novel Organotellurium Compound (RT-01) as a New Antileishmanial Agent

Camila Barbara Cantalupo Lima,Wagner Welber Arrais-Silva,Rodrigo Luiz Oliveira Rodrigue,Selma Giorgio 대한기생충학열대의학회 2009 The Korean Journal of Parasitology Vol.47 No.3