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        Co-immobilization of cholesterol esterase, cholesterol oxidase and peroxidase onto alkylamine glass beads for measurement of total cholesterol in serum

        Suman,C.S.Pundir 한국물리학회 2003 Current Applied Physics Vol.3 No.2, 3

        Commercially available cholesterol esterase, cholesterol oxidase and peroxidase have been co-immobilized onto alkylamine glassinitial activity. The co-immobilized enzyme system showed maximum activity at pH 7.0, when incubated at 37.C for 12 min. Amethod was developed for total serum cholesterol determination employing co-immobilized enzymes. There was a linear rela-tionship betweenA520 and cholesteryl acetate concentration ranging from 5mg to 50 mg/dl reaction mixture. The minimum de-tection limit of the method is 50 mg/dl. Within day and between day coecient of variation were< 1.0% and < 6%, respectively. Ar¼ 0:83) was found between the total serum cholesterol obtained by the present method and commercial Enzo-kitmethod employing free enzymes. Among the various serum substances tested at their physiological concentrations; Testosterone, vitD and progesterone caused 59%, 41% and 39% inhibition, while NaCl, KCl, CuSO4, creatinine, NaHCO3, albumin and estrogenhad practically no eect.. 2002 Elsevier Science B.V. All rights reserved.

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