韓國型 Turnip Mosaic Virus의 純化

김병훈,권오병,마병철,강미란,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.1

Chinese cabbage(Brassica campestris subsp pekinensis) useful for making a traditional Korean food, Kimchi, has been considered as the most important vegetable in Korea. In order to supply large quantity of chinese cabbage to the consumer, the mass cultivation is demanded. However, the mass production has been faced to serious problem due to fungal, bacterial and/or viral infections. It has been reported that the viral infections are the worst phatogeruc causes in a number of plants. In the chinese cabbage, turnip mosaic virus(TuMV) is the most serious pathogen. Five different strains of TuMV(Cl, C2, C3, C4, and C5) were identified in the world. We are reporting two strains(C4, C5) purified from Brassica nigra. They are all filamentous on electron micrographs. The TuMV C4 is about 1,300nm long, whereas the C5 is about 1,200nm long in average. They are larger than those reported previously(680-900nm). They will be used to produce monoclonal antibodies against the TuMV coat proteins. The antibodies arc required to develop a sensitive and specific screening methods for the TuMV infected plants.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

알레르기 뮤신을 지닌 만성 부비동염의 임상적 특징

동헌종,김성민,장병찬 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.3

마비형 뇌성마비환자의 뇌파 및 임상양상

윤여훈,송홍기,김형철,이병철,이숙자 대한신경과학회 1996 대한신경과학회지 Vol.14 No.1

유지 투석중인 만성 신부전환자에서 허혈성 심질환의진단 지표로서의 심장트로닌-I의 유용성

신병철,강대웅,정지용,류봉관,서영욱,김정인,김범윤,김현리,정종훈 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.1

Background : Coronary disease is highly prevalent in patient with end stage renal disease (ESRD) and account for much of their observed morbidity and mortality. Troponin-I consistently maintains a high sensitivity and specificity and is most sensitive marker for ischemic heart disease (IHD). Method : We examed 49 hernodialyzed patients (22 male, 27 female) without evidence of acute coronary syndrome (ACS) for 6 months. Biochemical markers were measured in serial predialysis blood samples. For analysis, we used two cardio-specific assays for troponin-T (cTnT) as well as for troponin-I (cTnI) and compared the results with CK-MB (reference value ≤ 4.0 ng/mL) concentration. Results : Myocardial ischemia was observed in 47% (23/49) of patients. cTnT level above 0.1 ng/mL. and cTnI level above 0.5 ng/mL, were observed in 22% (11/49) and 20% (10/49) of patients respectively. cTnI revealed significantly higher positive rate in patients with myocardial ischemia than the patients without myocardial ischemia (43 % vs 30%) (p<0.05). cTnT and CK-MB revealed no difference in positive rate between the patients with and without myocardial ischemia (cTnT : 30% vs 15% and CK-MB : 30% vs 19%). The sensitivity and specificity of cTnI to myocardial ischemia were higher than those of cTnT and CK-MB (sensitivity 43% vs 30% and 30%. specificity 100% vs 85% and 81%). Conclusion: Both cTnT and cTnI are useful in ruling out myocardial injury in chronic renal failure patients. But. cTnI is a more sensitivity and excellent specificity of ischemic heart disease than cTnT and CK-MB in hemodialyzed patients. In patients with ischemic heart disease, the presences of DM and advanced age were higher than those in patients without ischemic heart disease (p<0.05). Among the baseline characteristics old age, elevated LDH and diabeties were significant more frequent in the patients with elevation of cardiac troponin-I (p>0.5 ng/mL) than those with cardiac troponin-I (p<0.5 ng/mL), p=0.038, p=0.049, and p=0.045, respectively. Our results suggest that these cTnI is the potential diagnostic marker for the prediction of IHD in ESRD patients.

下水의 土地注入에 關한 硏究

李柄憲,姜壬錫 釜山水産大學校 1986 釜山水産大學 硏究報告 Vol.26 No.1-2

The objective of this study is to evaluate the land application of wastewater, which is applicable to small community without sewer systems. The laboratory units were consist of two-stage septic tanks, soil absorption systems and sois columns. Two-stage septic tanks were operated to evaluate pretreatment methods of soil absorption systems and soil column test was designed to examine the effect of hydraulic loading rates, organic loading rates and soil depths on the efficiencies of soil absorption systems. If land is available, land application of wastewater is a cost effective method in terms of construction and maintenance cost with high organics and nutrients removal efficiencies.

노인주거의 주요동선 안전과 조명의 역할에 관한 연구

宋秉夏,柳在憲 홍익대학교 산업기술연구소 2000 産業技術 Vol.10 No.-

Primary circulation areas such as corridors, stairs, ramp, and elevators are functionally the most essential portion in the building for most users. Nevertheless, these areas can present serious mobility difficulties, or even hazardous situations particularly for the elderly,if not designed properly. Properly designed, these areas are safe by providing helpful visual and tactile clues, as well as esthetically enjoyable. This study examines these areas in case of assisted living for the elderly. Based on the concept of Universal Design, detailed specifications and features that should confer safe circulation for the elderly in the areas, including lighting aspects, are included in the study.

筋力(背筋力)에 關한 調査硏究

蘇秉憲 서울敎育大學 1984 論文集 Vol.17 No.-

한국 현대소설의 문체 분류 시론

이병헌 고려대학교 민족문화연구원 한국문학연구소 2002 한국문학연구 Vol.- No.3

This is an attempt to set up the new criteria for classifying the style of the whole Korean modern novel.With the help of text liguistics, I set up the two dimensional standards which are 'presentation and expression' according to the functions of sentences and 'moderation and emission' according to the aspects of subject treatment, and looked into the characteristic of each style. As for the styles classified by the function of sentence, the style of 'presentation' is to write at a distance from the emotion, feeling and intention of the text maker or writer in describing the story, background and events of the novel and the characters' psychology. Whereas the style of 'expression' gives priority to the manifestation of the feeling and attitude of the writer and to the dealing with the text marker as a subject matter over the uninvolved netural writing.So the phrase which is intruded by the writer's judgement and the sentence which has the device that attracts the reader's attention are the style markers of the 'expression'. As for the styles classified by the aspects of subject treatment, the style of 'moderation' is the statement and expression which restrains the desire for using words and keeps the virtue of harmony and proportion in managing the subject of the novel.It's influence reveals when it shows the circumstances explicitly and makes clear the message that the writer wish to convey through a few proper words.The style of 'emission' is the narrator's attitude of saying freely and fully what he want to say in easy and relaxed stste with confidence. It is the style in which any circumstance and psychology is told fully by means of the rhetorical devices or the effect of communication can reach to the maximum by mean of eloquence, pungent colloquialism or unconstained sentence.

유식세포 분리분석기를 이용한 T 세포아군의 분석

安秉庸,鄭憲鐸 圓光大學校 基礎自然科學硏究所 1989 基礎科學硏究誌 Vol.8 No.1

단크론 항제 Anti Leu-3a, Anti Leu-2를 사용하여 유식세포 분리분석기를 이용한 정상인 30명과 간염환자 60명의 말초혈액 T 세포아군을 정량분석한 결과 다음과 같다. 1. 정상인의 말초혈액 림프구 중 T1^+, T4^+, T8^+, T4^+/T8^+ 비율은 각각 70.7±35%, 42.1±3.7%, 29.0±3.4%, 1.56±0.21%였다. 2. 바이러스성 간염환자에서 T4 양성세포 비율은 만성 바이러스 간염환자 보다는 급성 바이러스 간염환자에서 증가되는 경향을 보였으며, T8 양성세포비율은 급성 바이러스 간염환자 보다 만성 바이러스 간염환자에서 증가됨을 보였다. 3. 만성 간염환자에서 T1 양성세포가 급속히 감소됨을 보였다. 이와 같은 결과로 보아 간염환자의 면역기능이 발병학적인 T1 양성세포의 현저한 감소로 보아 만성간염환자의 방어기능은 NK(Natural Killer)세포가 주로 역할을 하는 것으로 사료된다. The impeortance of a balance between helper and suppressor cell in maintaining immune homeostasishas recently been illustrated and abnormalities in these T cell subsets have been associated with anumber of himan diseases. It is well known that immune respone to a virus may be regulated byimmumoregulatory network. In this study author analysed the subset of lymphocyte in peripheral bloodduring acute or chronic viral hepatitis. In healthy adults, the percentage of T1^+, T4^+,T8^+, and T4^+/T8^+ratio was 70 7±3.5%, 42.1±3.7%, 29.0±3.4%, and 1.56±0.21, respectively. In viral hepatitis, the percentage of T4^+ was tended to be increased in acute hepatitis than chronic hepatitis and the percentageof T8^+ was tended to be increased in chronic hepatitis than acute hepatitis. The percentage of t1^+was significantly decreased in chronic viral hepatitis than healthy adults. On the basis of these resultsthis study suggested that immunologic response in considered a likely pathogenic determinant in viralhepatitis and NK cells play a role in the host defence responses to chronic viral hepatitis.