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Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9
<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>
Byung Joo Sun,Jae-Hyeong Park,Minyeong Lee,최진오,Ju-Hee Lee,Mi-Seung Shin,Mi-Jeong Kim,Hae-Ok Jung,박정랑,Il Suk Sohn,Hyungseop Kim,Hyung-Kwan Kim,Goo-Yeong Cho,Jin-Sun Park,심지영,Sung Hee Shin,Kye Hun Kim,김 한국심초음파학회 2020 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.28 No.3
BACKGROUND: Left atrial (LA) strain is a novel parameter of LA function. However, its reference value has not been established, and the determining factors for LA strain remain elusive. We aimed to present LA strain with reservoir, conduit, and contractile components and associated parameters in a large-sized group of healthy individuals. METHODS: The present study was from a prospective multicenter registry in South Korea. Subjects who had no history of cardiovascular disease with adequate images were eligible for inclusion. LA reservoir, conduit, and contractile strains (LASRES, LASCD and LASCT, respectively) were measured. Left ventricular global longitudinal strain (LV GLS) and early and late diastolic strain rates (DSRe and DSRa, respectively) were also evaluated. RESULTS: Among a total of 324 subjects (mean age: 49 ± 16 years, 167 females), the mean LASRES, LASCD, and LASCT values were 35.9% ± 10.6%, 21.9% ± 9.3%, and 13.9% ± 3.6%, respectively. Mean LV GLS was -20.4% ± 2.2%, and mean DSRe and DSRa were 1.6 ± 0.4 s-1 and 0.8 ± 0.3 s-1, respectively. With aging, LASRES and LASCD showed significant decreases. Factors showing independent associations with LASRES were age (B = -0.425, p < 0.001), DSRe (B = 4.706, p = 0.001), and LV GLS (B = -1.081, p < 0.001). Age (B = -0.319, p < 0.001), DSRe (B = 4.140, p = 0.002), DSRa (B = -3.409, p = 0.018), and LV GLS (B = -0.783, p < 0.001) showed associations with LASCD. With LASCT, only DSRa showed a correlation (R = 0.277, p < 0.001). CONCLUSIONS: We presented LA strain in a large-sized group of healthy subjects. Age is a significant determinant of LA function. Associations of LA strain with diastolic strain rates and LV GLS reflect cardiac mechanics.
( Ok-hee Kim ),( Hyojung Kim ),( Jinku Kang ),( Dongki Yang ),( Yu-hoi Kang ),( Dae Ho Lee ),( Gi Jeong Cheon ),( Sang Chul Park ),( Byung-chul Oh ) 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.1
Accumulation of tissue macrophages is a significant cha-racteristic of disease-associated chronic inflammation, and facilitates the progression of disease pathology. However, the functional roles of these bone marrow-derived macrophages (BMDMs) in aging are unclear. Here, we identified age-dependent macrophage accumulation in the bone marrow, showing that aging significantly increases the number of M1 macrophages and impairs polarization of BMDMs. We found that age-related dysregulation of BMDMs is associated with abnormal overexpression of the anti-inflammatory interleukin-10. BMDM dysregulation in aging impairs the expression levels of pro-inflammatory cytokines and genes involved in B-cell maturation and activation. Phagocytosis of apoptotic Jurkat cells by BMDMs was reduced because of low expression of phagocytic receptor CD14, indicating that increased apoptotic cells may result from defective phagocytosis of apoptotic cells in the BM of aged mice. Therefore, CD14 may represent a promising target for preventing BMDM dysregulation, and macrophage accumulation may provide diagnostic and thera-peutic clues. [BMB Reports 2017; 50(1): 43-48]
External validation of IBTR! 2.0 nomogram for prediction of ipsilateral breast tumor recurrence
Byung Min Lee,Jee Suk Chang,Young Up Cho,Seho Park,Hyung Seok Park,Jee Ye Kim,Joo Hyuk Sohn,Gun Min Kim,Ja Seung Koo,Ki Chang Keum,Chang-Ok Suh,Yong Bae Kim 대한방사선종양학회 2018 Radiation Oncology Journal Vol.36 No.2
Purpose: IBTR! 2.0 nomogram is web-based nomogram that predicts ipsilateral breast tumor recurrence (IBTR). We aimed to validate the IBTR! 2.0 using an external data set. Materials and Methods: The cohort consisted of 2,206 patients, who received breast conserving surgery and radiation therapy from 1992 to 2012 at our institution, where wide surgical excision is been routinely performed. Discrimination and calibration were used for assessing model performance. Patients with predicted 10-year IBTR risk based on an IBTR! 2.0 nomogram score of 〈3%, 3%–5%, 5%–10%, and 〉10% were assigned to groups 1, 2, 3, and 4, respectively. We also plotted calibration values to observe the actual IBTR rate against the nomogram-derived 10-year IBTR probabilities. Results: The median follow-up period was 73 months (range, 6 to 277 months). The area under the receiver operating characteristic curve was 0.607, showing poor accordance between the estimated and observed recurrence rate. Calibration plot confirmed that the IBTR! 2.0 nomogram predicted the 10-year IBTR risk higher than the observed IBTR rates in all groups. High discrepancies between nomogram IBTR predictions and observed IBTR rates were observed in overall risk groups. Compared with the original development dataset, our patients had fewer high grade tumors, less margin positivity, and less lymphovascular invasion, and more use of modern systemic therapies. Conclusions: IBTR! 2.0 nomogram seems to have the moderate discriminative ability with a tendency to over-estimating risk rate. Continued efforts are needed to ensure external applicability of published nomograms by validating the program using an external patient population.
Kim, Hyeong Il,Kim, In Hee,Jeon, Byung Jun,Lee, Seok,Kim, Seong Hun,Kim, Sang Wook,Lee, Seung Ok,Lee, Soo Teik,Kim, Dae Ghon Kowsar 2012 Hepatitis monthly Vol.12 No.7
<P><B>Background</B></P><P>The prevalence of hepatitis C virus (HCV) infections in elderly patients has been increasing in a number of countries. A few reports concerning pegylated interferon-α (PEG-IFN-α)-based combination treatment in elderly chronic hepatitis C (CHC) patients have been published, with slightly different treatment outcomes.</P><P><B>Objectives</B></P><P>We investigated the treatment response and safety of PEG-IFN-α plus ribavirin combination therapy in elderly patients with CHC.</P><P><B>Patients and Methods</B></P><P>Among a total of 181 treatment-naïve CHC patients (60 patients with genotype 1, 121 patients with genotype 2 or 3), 38 were aged ≥ 65 years (defined as the elderly group) and 143 were aged < 65 years (defined as the non-elderly group).</P><P><B>Results</B></P><P>The overall sustained virologic response (SVR) was lower in the elderly group than in the non-elderly group, but it was not significantly different (65.8 % vs. 76.2 %, P = 0.15). In a subgroup analysis, among patients with genotype 1, the elderly group had a significantly lower SVR rate than the non-elderly group (30.8 % vs. 66.0 %, P = 0.03). However, the SVR rate in patients with HCV genotype 2 or 3 was comparable between the two groups (84.0 % vs. 81.3 %, P = 0.85). HCV genotype was significantly associated with SVR in the elderly patients (genotype 1 vs. 2 or 3, odds ratio: 0.18, 95% confidence interval: 0.000-0.869, P = 0.03). The incidence of premature discontinuation of treatment (21.1 % vs. 9.1 %, P = 0.05) and dose modification (52.6 % vs. 31.5 %; P = 0.02) due mainly to adverse events or laboratory abnormalities, were higher in the elderly group than in the non-elderly group.</P><P><B>Conclusions</B></P><P>PEG-IFN-α plus ribavirin combination therapy might be considered for elderly CHC patients, especially for genotype 2 or 3, with vigilant monitoring of adverse events.</P>
Prevalence of Bacterial Contaminations of Chicken Meat in South Korea 2014 - 2015
( Byung-kook Choi ),( Ok-mi Jeong ),( Chun-tae Lim ),( So-youn Youn ),( Na-young Kim ),( Byung-woo Jeon ),( Shin-ae Kim ),( Suk-chan Jung ),( Min-su Kang ) 대한인수공통전염병학회 2016 창립총회 및 학술대회 초록집 Vol.2016 No.1
Introduction: Food borne diseases are considered to begin from animal meat and meat products which are sometimes resulting to serious health problems due to the consumption of them. A two years study about bacteriological condition of chicken meat in South Korea, was carried out to investigate the occurrence of Salmonella spp., Staphylococcus aureus, Clostridium perfringens, Campylobacter coli, Campylobacter jejuni, Escherichia coli, Yersinia enterocolitica, and Listeria moncytogenes. Methods: Each solution of a hundred eighty samples of chicken carcasses was collected from the slaughter houses throughout the South Korea during 2014 -2015. The samples were analyzed for the presence of bacterial contaminations using Korean Food Standard Codex for meat and meat products. Results: In one hundred eighty chicken samples, Staphylococcus aureus (36.1%) is the most frequent pathogen in chicken meat for two years followed by Clostridium perfringens (31.7%), Campylobacter coli (23.3%), Salmonella spp. (13.3%), Campylobacter jejuni (10.0%), Escherichia coli: 0111 (3.9%), Escherichia coli: 0145 (2.2%), Escherichia coli: 026 (1.7%), Yersinia enterocolitica (1.1%), and Listeria monocytogenes (1.1%). Minor pathogens are Escherichia coli spp. (O45, O103, O104, O121 and O128). Conclusion: The bacteriological status of chicken meats from the slaughter houses showed that a variety of pathogens were able to transmit diseases to people especially high level of Staphylococcus aureus and Clostridium perfringens. Our findings suggest that farms and slaughter houses should more enforce hygiene system to reduce pathogens which are harmful for public health.
( Byung Gee Kim ),( Eun Ok Jung ),( Chang Min Sung ),( Beom Gi Park ),( Hyung Don Yun ) 한국피부장벽학회 2014 한국피부장벽학회지 Vol.16 No.2
ω-Hydroxy fatty acids (ω-OHFAs) are valuable chemicals for adhesives, lubricants, cosmetic intermediates and potential anticancer agents. Especially, ω-OHFAs have a potential as precursors for pseudo-ceramides. Although there are several processes for ω-OHFAs production by chemical synthesis, those have low yield and harsh reaction condition with high temperature. Here, we would like to discuss the production of ω -OHFAs using P450s which have the high regiospecificity to long chain fatty acids. To develop the biotransformation process for ω-OHFAs production, there are two major issues: enzyme engineering and host development. First, the several amino acids were substituted by site-directed mutagenesis to enhance the affinity for long-chain fatty acid based on bioinformatics tools. Second, because the omega-hydroxylation reaction is catalyzed by P450s with two electrons, we mimicked the self-sufficient P450 which is the most efficient electron transfer system in nature and the linker sequences were optimized. Third, cofactor regeneration system was developed to supply NAD(P)H in E.coli. Lastly, fadD (fatty acyl-CoA synthetase) which initiates cellular consumption of fatty acid was deleted and fadL (outer membrane long-chain fatty acid transporter) was over-expressed for substrate/product stability and substrate transport. Finally, 2.6 g/L (10.0 mM) of palmitic acid converted into 2.5 g/L (9.5 mM) of ω-hydroxy palmitic acid for 36hr with 0.07g/L/hr productivity. Also, ω-OHFAs were synthesized from glucose about 400mg/L.