韓國型 Turnip Mosaic Virus의 純化

김병훈,권오병,마병철,강미란,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.1

Chinese cabbage(Brassica campestris subsp pekinensis) useful for making a traditional Korean food, Kimchi, has been considered as the most important vegetable in Korea. In order to supply large quantity of chinese cabbage to the consumer, the mass cultivation is demanded. However, the mass production has been faced to serious problem due to fungal, bacterial and/or viral infections. It has been reported that the viral infections are the worst phatogeruc causes in a number of plants. In the chinese cabbage, turnip mosaic virus(TuMV) is the most serious pathogen. Five different strains of TuMV(Cl, C2, C3, C4, and C5) were identified in the world. We are reporting two strains(C4, C5) purified from Brassica nigra. They are all filamentous on electron micrographs. The TuMV C4 is about 1,300nm long, whereas the C5 is about 1,200nm long in average. They are larger than those reported previously(680-900nm). They will be used to produce monoclonal antibodies against the TuMV coat proteins. The antibodies arc required to develop a sensitive and specific screening methods for the TuMV infected plants.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

알레르기 뮤신을 지닌 만성 부비동염의 임상적 특징

동헌종,김성민,장병찬 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.3

蔓蔘의 methanol 추출액이 mitomycin C의 세포독성 효과에 미치는 영향

田炳薰,鄭遇悅 한국전통의학연구소 1997 한국전통의학지 Vol.7 No.2

만삼은 인삼의 대용으로도 사용할 수 있는 약물로서 가능성이있고,수요가 많은 인삼을 대체할 수 도 있는 약물로 생각된다. 인삼에 대한 임상적 연구와 항암효과에 대한 연구는 많이 있으나 만삼에 대한 연구는 그리 많지 않았다. 그러나 최근에 만삼의 다양한 임상적 효능과 항암효과에 관한 실험등이 중국등을 중심으로 많이 발표 되었다. 蔓蔘의 항암효과는 암세포의 동물이식에 대한 실험,화학적 발암원에 의한 육종에 대한 효과,배양한 종앙세포 등에 대한 실험에서 많이 조사되었다. 蔓蔘에 대한 많은 연구가 있었으나 복강내주사나 in vitro 모델등에서 실시한 것이었고,경구투여를 통한 실험은 거의 없었다. 한편 정상세포에는 손상을 주지않고 오직 암세포만을 살상 하는 항종양성약물은 아직까지 개발되지 않았다. 따라서 항종양성약물의 항종양효과를 상승적으로 증진시키고 부작용을 줄이는 약물의 개발은 절실한 것이다. 때문에 본 논문에서는 Ehrlich carcinoma의 solid tumor와 ascites tumor에 대한 蔓蔘추출물의 항종양효과를 관찰하였다. 蔓蔘추출물이 항암화학요법제인 MMC의 항종양효과를 증가시킬 수 있는가를 살피고,종양세포로의 MMC의 uptake,종양세포의 lysosomal enzymes에 대한 활성 및 MMC의 세포독성에 어떠한 효과를 나타내는 가를 관찰하였다. The influence of the ethanol extract from RADIX CODONOPSITIS(RC) on the lysosome of tumor cells and on the cytocidal effect of mitomycin C(MMC) was investigated. KRG treatment showed an inhibitory effect on the solid form of Ehrlich ascites carcinoma but had no effect on the ascites form. Mitomycin C combined with RADIX CODONOPSITIS(RC) showed stronger antitumor effects at the same time, the activity of lysosomal enzymes in tumor cells was also increased in comparison with that treated with MMC alone. Furthermore, RADIX CODONOPSITIS(RC) promoted the uptake of MMC into the tumor cells and enhanced in vitro the cytotoxicity of MMC against the cultured tumor cells. From these results it was concluded that RADIX CODONOPSITIS(RC) labilized the Iysosomes of tumor cells in vivo, and increased the uptake if MMC into the tumor cells, and that the cytocidal effect of MMC was enhanced by concomitant treatment with RADIX CODONOPSITIS(RC).

청소년비행과 성격양상 및 가족역동간의 관계

김헌수,민병근,김현실 울산대학교 의과대학 1992 울산의대학술지 Vol.1 No.1

The present study was intended to explore the correlation between juvenile delinquent behavior and their character, mood state and family dynamics. Data were collected through questionnaire survey. Subjects served for this study consisted of 1,838 adolescents including 601 juvenile delinquents and 1,237 adolescent students in Korea, sampled from Korean student population and adolescent delinquent population confined in juvenile correctional institutions, using nonprobability samping method. Their age ranged between 12 and 21. The actual number of cases put into the analysis was 1,696 adolescents including 511 delinquents and 1,185 adolescent students. Data were analysed by IBM PC of Behavioral Science Center at the Korea University, using SAS program. Statistical methods employed were χ²test, simple correlation, principal component analysis, principal component regression analysis and LISREL path analysis. The results of this study were follows: 1. Inconsistent by parental child rearing patterns tended to affect delinquent behavior among delinquent adolescents. The more were paternal and maternal attitudes arbitrary, restricted, mistrusing their children and punishment-oriented, the higher occured their children's delinquent behavior. On the other hand, adolescent students were consistently rearing by their parents, their parental attitudes were democratic, flexible, trusting their children and reward-oriented. In comparison of both parents in the degree of influence on their children was revealed that paternal child rearing pattern was more influential on their children's behaviors than maternals's. 2. The psychological instability of family tended to be contributing to deliquent behaviors among deinquent adolescents. Coldness, dissatisfaction, discord, tenseness and disharmony of family paychological climate revealed significant correlation with delinquent behaviors. On the other hand, student's family stability was more comforable than delinquent family. 3. The disharmonious parent-child relationships were presented to influence on delinquent behaviors and aggressive impulsiveness. Parental rejection toward their children's opinions, lack of affection toward their children, non-self-sacrificing for their children, alienated parent-child relationship, children's disregard toward their parental authority and children's rejection of parental opinions were positively correlated to behavioral problems among delinquent adolescents, while harmonious parent-child relationships among student adolescents were maintained. 4. The whole psychological climates of delinquent's family tended to be rigid, while student's psychological climates of family flexible. 5. Delinquents were more anxious, aggressive and impulsive than student adolescents. They exhibited some learning problems such as interpersonal difficulties. their character patterns tended to be immature and weak ego functioning, while student's character patterns more mature and flexible. 6. The relative comparison of the whole psychological climates of family, anxiety trait, character pattern and social learning ability in terms of predictability revealed that character pattern variables function as the most important determinant of both aggressive impulsiveness and delinquent(aggressive) behaviors.

응급실에서 급성 췌장염 환자의 선별을 위한 actim pancreatitisR검사의 유용성

송석훈,도병수,이삼범 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Acute pancreatitis is a fairly common abdominal disorder that can be easily confronted in the emergency department(ED) and is one of the most complex and clinically challenging of all abdominal disorders. Currently, there is no gold standard for the diagnosis of acute pancreatitis. We evaluated the usefulness of the actim Pancreatitis□ rapid test, which has been recently developed in Europe, as a screening test of acute pancreatitis in our emergency department. Methods: We prospectively studied the urinary trypsinogen-2 dipstick tests(actim Pancreatitis□, Medix Biochemica, Kauniainen, Finland) of 200 patients with acute abdominal pain who were treated at our ED. All urine samples were collected from the patients within 12 hours of arrival at ED. We also measured serum amylase assay in the laboratory and compared the sensitivities and the specificities of the two test. Acute pancreatitis was diagnosed by authors according to standardized criteria. Abdominal USG or CT was performed when a patient with a normal serum amylase level had a typical clinical presentation. Results: The actim Pancreatitis□ rapid test was positive in 22 of the 25 patients with acute pancreatitis(sensitivity, 88.0 percent). The test was also positive in 6 of 175 patients without pancreatitis(specificity, 96.6 percent). The serum amylase assay had a sensitivity of 68.0 percent(with a cutoff value of 300 U per liter for the upper reference limit) and a specificity of 90.9 percent. But there were no statistical differences between the correlation coefficients of the two tests as diagnostic tool Conclusion: In patients with acute abdominal pain who are seen in the emergency department, the actim Pancreatitis□ urinary dipstick test has a high degree of probability as a screening test for acute pancreatitis. Also it can easily and rapidly be use at the bedside.

학생청소년의 불안성향과 문제행동 유형간의 상관분석

김헌수,이재우,나철,김영돈,김민호,김은희,권지란,박광식,이길홍,민병근 중앙대학교 의과대학 의과학연구소 1987 中央醫大誌 Vol.12 No.4

The purpose of the present study was to examine the relationship between their problem behavioral pattern and anxious trends among Korean student adolescent. Zung's measurement of anxiety level undergone through used of the self-rating anxiety scale. the data were collected through the questionaire survey which was conducted on Dec.1, 1983. Subjects served for this atudy consisted of 1,404 syudents in Korea, randomly sampled from Korean student population by means of census method. The data were analysed by CDC-174 computer of KAIST, using SPSS pogram. Statistical methods empoyed were χ^2 test and Eta measures. the results of this study were as follows. 1. It showed that students psychological anxious trends had greatest significant correlation with adolescent student's problem brhavioral pattern, but the cardiovascular, respiratory, gastrointestional and genitourinary anxious trends has not significantly related with student's problem behavioral pattern. 2. Students group with undersocialized aggressive, undersocialized nonaggressive,undersocialized school-norm avoidant, or socialized nonaggressive behavioral patterns were less anxious, whereas those with socialized latent aggressive, socialized sexual, or socialized modellong behavioral patterns were higher anxious.

한국 정신 역동학실제의 현황 분석

김헌수,김현실,민병근 울산대학교 의과대학 1993 울산의대학술지 Vol.2 No.1

This study was intended to get the base-line data of psychodynamic formulation for teaching the residents through the analysis of the current status of psychodynamic formulation in Korea. Data were collected through the mailings-questionnaire survey. Subjects served for this study consisted of 1,034 psychiatrists including 259 residents and 775 psychiatric specialists in Korea, using census method. Statistical method employed was percentage rate. 1. 267 respondents(25.8%) among 1,034 psychiatrists replied to our mailings questionnaire. 2. 83.5 percent of respondents perceived psychodynamic formulation to be very important, 46.8 percent of respondents felt formulation to be crucial for second year residents, while 37.8 percent of respondents stated that they referred to textbooks of psychiatry, others(25.1 percent) sent guidelines for the mental status examination and/or descriptions of the intake interview. 5.6 percent of respondents did not provide any materials for psychodynamic formulation. 2 percent of respondents did not answer this question. 55.4 percent of respondents recognized general psychiatry, psychopharmacology and biological psychiatry to be more important than the teaching of formulation. As a result, though the Korean psychiatrists recognized formulation to be very important, they did not sufficiently provide the materials about psychodynamic formulation. 3. the Korean psychiatrists prefered to psychoanalysis models, focal psychodynamic approaches and/or biopsychosocial approaches in case of formulating the psychiatric patients, while the American psychiatrists tended to adapt psychoanalysis and/or ego psychological models to their patients. 4. 72.7 percent of respondents indicated that they distinguished psychodynamic formulation and diagnostic assessment, while 20.9% of respondents emphasized that all patients require a diagnostic assessment. 48.7 percent of respondents viewed that psychodynamic tended to be overlooked and declined due to recent advanced biological psychiatry.

과학적 실재론과 포스트모던 과학철학 : 파인의 "자연적인 존재론적 태도"를 중심으로

정병훈 영남철학회 1996 哲學論叢 Vol.12 No.-

유식세포 분리분석기를 이용한 T 세포아군의 분석

安秉庸,鄭憲鐸 圓光大學校 基礎自然科學硏究所 1989 基礎科學硏究誌 Vol.8 No.1

단크론 항제 Anti Leu-3a, Anti Leu-2를 사용하여 유식세포 분리분석기를 이용한 정상인 30명과 간염환자 60명의 말초혈액 T 세포아군을 정량분석한 결과 다음과 같다. 1. 정상인의 말초혈액 림프구 중 T1^+, T4^+, T8^+, T4^+/T8^+ 비율은 각각 70.7±35%, 42.1±3.7%, 29.0±3.4%, 1.56±0.21%였다. 2. 바이러스성 간염환자에서 T4 양성세포 비율은 만성 바이러스 간염환자 보다는 급성 바이러스 간염환자에서 증가되는 경향을 보였으며, T8 양성세포비율은 급성 바이러스 간염환자 보다 만성 바이러스 간염환자에서 증가됨을 보였다. 3. 만성 간염환자에서 T1 양성세포가 급속히 감소됨을 보였다. 이와 같은 결과로 보아 간염환자의 면역기능이 발병학적인 T1 양성세포의 현저한 감소로 보아 만성간염환자의 방어기능은 NK(Natural Killer)세포가 주로 역할을 하는 것으로 사료된다. The impeortance of a balance between helper and suppressor cell in maintaining immune homeostasishas recently been illustrated and abnormalities in these T cell subsets have been associated with anumber of himan diseases. It is well known that immune respone to a virus may be regulated byimmumoregulatory network. In this study author analysed the subset of lymphocyte in peripheral bloodduring acute or chronic viral hepatitis. In healthy adults, the percentage of T1^+, T4^+,T8^+, and T4^+/T8^+ratio was 70 7±3.5%, 42.1±3.7%, 29.0±3.4%, and 1.56±0.21, respectively. In viral hepatitis, the percentage of T4^+ was tended to be increased in acute hepatitis than chronic hepatitis and the percentageof T8^+ was tended to be increased in chronic hepatitis than acute hepatitis. The percentage of t1^+was significantly decreased in chronic viral hepatitis than healthy adults. On the basis of these resultsthis study suggested that immunologic response in considered a likely pathogenic determinant in viralhepatitis and NK cells play a role in the host defence responses to chronic viral hepatitis.