광중합형 글라스아이오노머 시멘트와 복합레진과의 전단결합강도에 관한 연구

김덕,민병순,최호영,박상진,최기운 慶熙大學校 齒科大學 1994 慶熙齒大論文集 Vol.16 No.1

The purpose of this study is to evaluate of shear bond strength of light-curing composite resin to light-curing glass ionomer cement. Composite resin and glass ionomer cement have been widely used as an esthetic filling materials in dental clinics. To achieve better clinical results, sandwich technic was developed with conpensating for disadvantages of these two materials. Especially, light-curing glass ionomer cement provided greately improved bonding strength of teeth or composite resin, and then excellent clinical results can be acquired. In this study, 6 commercial light-curing glass ionomer cements(3 commercial restorative materials Fuji II LC, Vanglass VLC, Vitremer, and 3 commercial lining materials : Fuji Lining LC, Baseline VLC, Vitrebond) were devided two groups. According to manufacturer's appointment, no surface treatment was referred to N groups. Supposing of clinical practice, surface grinding with water ;spray at 320 grit sand paper, 40 seconds etching with 37% phosphoric acid, 20 seconds washing 20 seconds air drying was referred to E groups. Totally 12 experimental groups were devided, and all 120 specimens from 10 specimens of each groups were made. After light-curing composite resin was bonded to right-curing glass ionomer cement, shear bond strength was tested by Instron universal testing machine between glass ionomer cement and composit resin. The data were analyzed statistically by Student's t-test and ANOVA. The obtained results were as follows; 1. In light-curing glass ionomer cement, restorative materials showed higher shear bond strength to composite resin than lining matenals(p<0.05). 2. Variglass VLC of restorative material group and Baseline VLC of lining material group have highest shear bond strength to composite resin(p<0.001). 3. In light-curing glass ionomer cement:, surface grinding and acid etching reduced shear bond strengthto composite resin(p<0.001). 4. VGN group is highest shear bond strength to composite resin, VBE group is lowest shear bond strength to composite resin(p<0.001).

휠체어 검도 경기를 위한 경기규정에 관한 연구

김영학,공병덕,이병기 龍仁大學校 武道硏究所 2002 武道硏究所誌 Vol.13 No.1

The purpose of this study was to build revised game rules for wheelchair Kumdo for the physically challenged confined to wheelchair, after teaching 10 people how to play Wheelchair Kumdo. It's ultimately intended to determine whether or not the revised game regulations were appropriate and workable and ultimately to promote Wheelchair Kumdo. There Kumdo trainees of the 4th, 5th and 6th grades respectively and three physically different people were coached to draft game rules in three phases, and they were finalized through an advice of a specialist. And then five disabled Kumdo trainees, who included three of the 3rd grade and two of the 2nd grade, learned the regulations for a month to find out their adequacy. As a result of interviewing them and analysing the collected data, the following findings were given: First, the stadium should have a flat floor, by using proper materials such as wood or concrete, to guarantee the smooth operation of wheelchair. It should be in a square form and 11 meter or higher in length. The starting lines should bo marked 'T' on both sides from the central line, and the upper parts of the marks should face each other. There should be 1.7m distance between the central and starting lines and 2 meter or higher extra spaces in the external sides. Second, the bamboo sword should be redesigned to be 114cm in length and 370g in weight to fit in wheelchair Kumdo. Third, among Kumdo equipment, Homyun(護面, Headgear), part of HoKu(Protection-equipment, 護具), should be made of lighter materials, thicker in the head part, and easy to wear. Gab(甲, Chest-guard) should have a sort of shock absorber in it, and a remodeled Hoku that has no wings on both sides should be used. To prevent wheelchair from shock and ensure its safety, a Quickie All Court model should be selected, and each contestant should wear a suit of different color that has his or her name on the shoulder portion. Fourth, each game should run four minutes, constituted by two periods, and a break time should be given. The judgment should be made without no extra time, and a contestant who hits one of the due points ahead of the other should be a winner. Fifth, as for Yuhyo-Geokja(有效擊刺, Point), action against the head, wrist or waist should be considered Geokja(擊刺, Strike), and one against the chest part should be regarded as Jabu(刺部, Stab). Based on the above-mentioned findings, there are some suggestions about wheelchair Kumdo games: First, as games could be held out in the field, outdoor programs should be attempted. Second, every game is divided into the first and second periods, and coaches need to develop programs suitable for the two-part competition to give better guidance. Third, general Kumdo equipment isn't suitable for wheelchair Kumdo, and a lot of research efforts should urgently be dedicated to developing separate equipment for that.

광중합형 글라스아이오노머 시멘트와 복합레진의 전단결합강도에 관한 연구

김덕,민병순 大韓齒科保存學會 1994 Restorative Dentistry & Endodontics Vol.19 No.2

아르곤 레이저에 의한 복합레진의 중합시 물성 변화에 관한 연구

김덕,민병순,최호영,최기운,박상진 大韓齒科保存學會 1998 Restorative Dentistry & Endodontics Vol.23 No.1

After polymerizing composite resin with argon laser and visible light, four test, to be concretelv, measurement of compressive strength using Instron testing machine, surface microhardness using Rockwell hardness tester, quantitative analysis of residual monomer using HPLC and analysis of degree of conversion using FTIR, were accomplished. Test groups were a sort of specimen with 3mm diameter, 4㎜ thickness for measuring compressive strength, two sort of specimen with 7mm diameter, 2mm and 3㎜ thickness for measuring surface microhardness, quantitative analysing of residual monomer after curing and measuring the degree of conversion, each were divided by according to the condition of light exposure. In case of argon laser. in 1.0W and 0.5W output. the exposure time for specimen were 5 sec, 10 sec respectiyely. In case of visible light, the exposure time obtained. 1. Compressive strength of composite resin was the highest in the group of 1W output, exposing for 10 sec with argon laser, followed by the group of 0.5W, exposing for 10 sec with argon laser, the group of exposing for 40 sec with visible light. But there were statistically no significant difference between these three groups(p>0.05). 2. Surface microhardness of composite resin wasn't significantly affected by light curing conditions. 3. BIS- GMA within residual monomer was least detected in the group of exposing for 40 sec. TEGDMA was least detected in the group of 1W output, exposing for 10 sec with argon laseboth 2㎜ and 3㎜ thickness specimen. 4. The degree of conversion of all groups in the 2㎜ thickness specimen were more than 5%, similar to each other but in the group of 1W, exposing 10 sec with argon laser the degree of conversion was highest in the 3㎜ thickness specimen. 5. Argon laser could make composite resin to has similar properties with 25% lesser exposure time than visible light.

Polylysine을 입힌 배양면이 GH₃ 뇌하수체 종양 세포에 미치는 영향에 관한 연구

김정진,백상호,황덕호,황영일,이왕재,이병란 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.3

Simple Sequence Repeat Primer에 의한 딸기의 Genomic DNA 분석

양덕춘,최성민,김무성,민병훈 경희대학교 생명자원과학연구원 2002 硏究論文集 Vol.22 No.-

본 연구는 진핵세포에 널리 산재되어있는 repeat sequence를 primer로 사용하여 딸기의repeat sequence 좌위를 증폭시켜서 분석하고 이를 같은 종일지라도 지역간의 차이로 인한 환경의 변화 또는 재배방법의 차이에 의해서 일어나는 변이를 분석하는 방법에 활용할 수 있도록 하기 위하여 수행하였다. GGC repeat sequence의 딸기 genomic DNA에 존재여부를 판명하기 위하여 (GGC)_(6) probe를 DIG을 사용하여 labeling하여 딸기 genomic DNA에 hybridization한 결과 확실한 spot이 나타났다. 또한 repeat sequence로 이루어진 UBC SSR primer 100개중에서 4가지 서로 다른 primer에서 선명한 band를 확인할 수 있어서 딸기의 genomic DNA내에 여러 종류의 repeat sequence가 있는 것으로 판단되었다. 따라서 각 품종별 표지로서 이용성을 알아보고자 PCR을 수행하였는 바 (ACC)_(6)와 (CCG)_(6)의 primer에서는 다른 band에 비해 매우 확실한 밴드를 형성하였다 이러한 PCR product의 염기서열분석을 위해 우선 대량증식을 위한 PCR의 적정 조건은 primer 10p㏖, DNA 50ng으로서 수행하는 것이 DNA의 증폭에 좋았다. 증폭된 1 Kb DNA 절편을 이용하여 염기서열을 조사하였던 바, 808bp의 서열을 알아냈고 그 반복염기서열은 효모와 사람에 존재하는 유전자이었으며 Cdontella sinensis와의 유의성 검정결과 ACC 반복염기에 62% 동일성을 나타내었다. This study was carried out to investigate genetic variation caused by environmental change or cultural method difference, which was performed by amplifying repeat sequence loci of strawberry widely dispersed in eukaryotic cell. DIG labeled(GGC)_(6), probe was used to test if GGC repeat sequence exists in strawberry genomic DNA, resulting in clear spot on the filter. Among the 100 UBC SSR primers which contain repeat sequence, four different primers amplified clear DNA fragments, suggesting that the repeat sequence exist in the genome of strawberry. PCR was also performed to test whether these primers could be used for a marker among strawberry cultivars. (ACC)_(6), (No. 861) and (CCG)_(6) (No. 865) primers showed very clear bands compared to the other primers. The amplified 808 bp of 1 kb DNA was sequenced, and database search using the sequence information showed that the repeat sequence was the gene existed both in yeast and human, and a significant sequence homology to Odontella sinensis was found.

Roles of Ile66 and Ala107 of d-psicose 3-epimerase from Agrobacterium tumefaciens in binding O6 of its substrate, d-fructose

Kim, Hye-Jung,Lim, Byung-Chul,Yeom, Soo-Jin,Kim, Yeong-Su,Kim, Dooil,Oh, Deok-Kun Springer-Verlag 2010 Biotechnology letters. Vol.32 No.1

Risk Factors and Preoperative Risk Scoring System for Shunt-Dependent Hydrocephalus Following Aneurysmal Subarachnoid Hemorrhage

Kim, Joo Hyun,Kim, Jae Hoon,Kang, Hee In,Kim, Deok Ryeong,Moon, Byung Gwan,Kim, Joo Seung The Korean Neurosurgical Society 2019 Journal of Korean neurosurgical society Vol.62 No.6

Objective : Shunt-dependent hydrocephalus (SdHCP) is a well-known complication of aneurysmal subarachnoid hemorrhage (SAH). The risk factors for SdHCP have been widely investigated, but few risk scoring systems have been established to predict SdHCP. This study was performed to investigate the risk factors for SdHCP and devise a risk scoring system for use before aneurysm obliteration. Methods : We reviewed the data of 301 consecutive patients who underwent aneurysm obliteration following SAH from September 2007 to December 2016. The exclusion criteria for this study were previous aneurysm obliteration, previous major cerebral infarction, the presence of a cavum septum pellucidum, a midline shift of >10 mm on initial computed tomography (CT), and in-hospital mortality. We finally recruited 254 patients and analyzed the following data according to the presence or absence of SdHCP : age, sex, history of hypertension and diabetes mellitus, Hunt-Hess grade, Fisher grade, aneurysm size and location, type of treatment, bicaudate index on initial CT, intraventricular hemorrhage, cerebrospinal fluid drainage, vasospasm, and modified Rankin scale score at discharge. Results : In the multivariate analysis, acute HCP (bicaudate index of ${\geq}0.2$) (odds ratio [OR], 6.749; 95% confidence interval [CI], 2.843-16.021; p=0.000), Fisher grade of 4 (OR, 4.108; 95% CI, 1.044-16.169; p=0.043), and an age of ${\geq}50years$ (OR, 3.938; 95% CI, 1.375-11.275; p=0.011) were significantly associated with the occurrence of SdHCP. The risk scoring system using above parameters of acute HCP, Fisher grade, and age (AFA score) assigned 1 point to each (total score of 0-3 points). SdHCP occurred in 4.3% of patients with a score of 0, 8.5% with a score of 1, 25.5% with a score of 2, and 61.7% with a score of 3 (p=0.000). In the receiver operating characteristic curve analysis, the area under the curve (AUC) for the risk scoring system was 0.820 (p=0.080; 95% CI, 0.750-0.890). In the internal validation of the risk scoring system, the score reliably predicted SdHCP (AUC, 0.895; p=0.000; 95% CI, 0.847-0.943). Conclusion : Our results suggest that the herein-described AFA score is a useful tool for predicting SdHCP before aneurysm obliteration. Prospective validation is needed.

Alpha-Pinene Exhibits Anti-Inflammatory Activity Through the Suppression of MAPKs and the NF-κB Pathway in Mouse Peritoneal Macrophages.

Kim, Dae-Seung,Lee, Hyun-Ja,Jeon, Yong-Deok,Han, Yo-Han,Kee, Ji-Ye,Kim, Hyun-Jeong,Shin, Hyun-Ji,Kang, JongWook,Lee, Beom Su,Kim, Sung-Hoon,Kim, Su-Jin,Park, Sang-Hyun,Choi, Byung-Min,Park, Sung-Joo,U Institute for Advanced Research in Asian Science a 2015 The American journal of Chinese medicine Vol.43 No.4

<P>In this study, we found that alpha-pinene (α-pinene) exhibits anti-inflammatory activity through the suppression of mitogen-activated protein kinases (MAPKs) and the nuclear factor-kappa B (NF-κB) pathway in mouse peritoneal macrophages. α-Pinene is found in the oils of many coniferous trees and rosemary. We investigated the inhibitory effects of α-Pinene on inflammatory responses induced by lipopolysaccharide (LPS) using mouse peritoneal macrophages. α-Pinene significantly decreased the LPS-induced production of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and nitric oxide (NO). α-Pinene also inhibited inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions in LPS-stimulated macrophages. Additionally, the activations of MAPKs and NF-κB were attenuated by means of α-pinene treatment. These results indicate that α-pinene has an anti-inflammatory effect and that it is a potential candidate as a new drug to treat various inflammatory diseases.</P>

Nanotopographical regulation of pancreatic islet-like cluster formation from human pluripotent stem cells using a gradient-pattern chip

Kim, Jong Hyun,Park, Bo Gi,Kim, Suel-Kee,Lee, Dong-Hyun,Lee, Gyung Gyu,Kim, Deok-Ho,Choi, Byung-Ok,Lee, Kyu Back,Kim, Jong-Hoon Elsevier 2019 Acta Biomaterialia: structure-property-function re Vol.95 No.-

<P><B>Abstract</B></P> <P>Bioengineering approaches to regulate stem cell fates aim to recapitulate the <I>in vivo</I> microenvironment. In recent years, manipulating the micro- and nano-scale topography of the stem cell niche has gained considerable interest for the purposes of controlling extrinsic mechanical cues to regulate stem cell fate and behavior <I>in vitro</I>. Here, we established an optimal nanotopographical system to improve 3-dimensional (3D) differentiation of pancreatic cells from human pluripotent stem cells (hPSCs) by testing gradient-pattern chips of nano-scale polystyrene surface structures with varying sizes and shapes. The optimal conditions for 3D differentiation of pancreatic cells were identified by assessing the expression of developmental regulators that are required for pancreatic islet development and maturation. Our results showed that the gradient chip of pore-part 2 (Po-2, 200–300 nm diameter) pattern was the most efficient setting to generate clusters of pancreatic endocrine progenitors (PDX1+ and NGN3+) compared to those of other pore diameters (Po-1, 100–200 or Po-3, 300–400 nm) tested across a range of pillar patterns and flat surfaces. Furthermore, the Po-2 gradient pattern-derived clusters generated islet-like 3D spheroids and tested positive for the zinc-chelating dye dithizone. The spheroids consisted of more than 30% CD200 + endocrine cells and also expressed NKX6.1 and NKX2.2. In addition, pancreatic β- cells expressing insulin and polyhormonal cells expressing both insulin and glucagon were obtained at the final stage of pancreatic differentiation. In conclusion, our data suggest that an optimal topographical structure for differentiation to specific cell types from hPSCs can be tested efficiently by using gradient-pattern chips designed with varying sizes and surfaces.</P> <P><B>Statement of Significance</B></P> <P>Our study provides demonstrates of using gradient nanopatterned chips for differentiation of pancreatic islet-like clusters.</P> <P>Gradient nanopatterned chips are consisted of two different shapes (nanopillar and nanopore) in three different ranges of nano sizes (100–200, 200–300, 300–400 nm). We found that optimal nanostructures for differentiation of pancreatic islet-like clusters were 200–300 nm nano pores.</P> <P>Cell transplantation is one of the major therapeutic option for type 1 diabetes mellitus (DM) using stem cell-derived β-like cells. We generated 50 um pancreatic islet-like clusters in size, which would be an optimal size for cell transplantation. Futuremore, the small clusters provide a powerful source for cell therapy.</P> <P>Our findings suggest gradient nanopatterned chip provides a powerful tool to generate specific functional cell types of a high purity for potential uses in cell therapy development.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>