Effect of Siryungtang, Steroid, Interferon-γand Cyclosporin-A on Mitogen Induced Interleukin-4 Activity in Minimal Change Nephrotic Syndrome

Cho, Byoung Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Byoung-Soo cho. Department of Pediatrics, College of Medicine, Kyung Hee University, Seoul, Korea. Effect of Siryungtang, Steroid, Interferon-γand Cyclosporin-A on Mitogen Interleukin4-Activity in Minimal Change Nephrotic Syndrome. Proceedings of International Symposium on East-West Medicine, Seoul. 183-186, 1999. -We have reported that patients with childhood minimal change nephrotic syndrome (MCNS) express higher levels of IL-4 mRNA expression by mitogen activated T cells using Northern blot(Cho,et al, JASN 1:518,2:590). Siryungtang(SA-1) is an oriental medicine shich has long been used in chronic renal diseases. In order to evaluate the efficacy of SA-1 on IL-4 sctivity from the monouclear cells isolated from the MCNS patients(n=5) and controls(n=5). Cells were adjusted to 2×107cells/ml with 10% FCS-RPMI and added test drugs such as SA-1, steroid(hydrocortisone, HC), CsA, IL-4& combination of SA-1+HC,SA-1+CsA&SA-1+IFN-γand mitogens(PHA-P:20㎕/ml, Con-A:20㎕/ml & PMA:50ng/ml) were added. Cells were cultured at 37℃,5% CO2 incubator for 24,48 & 72hrs. Results were as follows. 표 Mean ³[H]thymidine incorporation(cpm) was higher in controls(44,920±7,482cpm)that in patients with MCNS(30,495±2.135). However ³[H]thymidine incorporation was reduced by adding SA-1,HC, CsA & IFN-γ,or which CsA was the most effective, by combination treatment with SA-1 to steroid and CsA, significant additive effects were noted in MCNS group. In conclusion SA-1 has a steroid and CsA sparing effect and could reduce side effects of steroid and Cas significantly if clinically applicated.

안구적출에 따른 위둔덕의 칼슘결합단백질의 재구축 및 상호 연관성

안병수,고길석,안명수,김경주,권안성,정명섭,박춘매,조병옥,김진우,Samudra Acharya,Parmeshwar Narayan Amatya,장인엽 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Background: Superior colliculus is a part of midbrain, and participates in the visual reflexes, It receives afferent fibers from optic nerve, visual cortex, and spinotectal tract. After optic deprivation, the microscopic structure of the superior colliculus changed. Calcium-binding proteins (CBPs) Play an important role in the neuronal protection, differentiation and reorganization of the central nervous system, Objectives and Methods: The effects of neonatal retinal deafferentation on a CBPs, calbindm D-28k (CB), Parvalbumin (PB) and calretimn (CR), and the existence of colocalization between the CBPs were examined immunohistochemically in the rat superior colliculus. Results: On the experimental (contralateral to enucleation) side of superior colliculus, the number of CB-immunoreactive (IR) cells was reduced (77.4% compared to control), but not fibers. The number of PB-IR neurons and fibers was also reduced on the experimental side (88.5% compared to control), In the other hand, the CR-IR cells were dramatically increased (642% compared to control), but CR-IR fibers were markedly decreased on the experimental side. The colocalization between CB-CR and PV-CR was rarely observed in the superior colliculus Conclusion: These results suggest that the changes of retinotectal projection may alter the expressional pattern of CBPs in different manners; relatively stable in CB- and PV-IR neurons and plastic in CR-IR neurons.

Android를 이용한 원격조종 어드벤처 게임 개발

안병호, 안성옥, 김수균 배재대학교 공학연구소 2013 공학논문집 Vol.15 No.1

In this paper, we introduce a two game programs and device controller installed programs remotely playable game. The existing joystick and the game screen is integrated to the smart phone game users feel uncomfortable from another device to disconnect the joystick control was designed. Games and controllers of this new system and it could lead to a new and fun game of the inconveniences of the existing games can be improved.

난소암에서 CA125의 임상적 의의

윤병일,김수녕,손인숙,유연웅,최원석 건국대학교 의과학연구소 2000 건국의과학학술지 Vol.10 No.-

The concentration of the tumor marker CA125 in serum from patients with ovarian tumor was measured to define the usefulness of them in differentiating malignant ovarian tumor from benign ovarian mass. In the study of 105 patients with gynecologic neoplasm, of whom 38 were myoma uteri, 42 benign ovarian tumor, and 25 ovarian cancer, the level of serum CA125 were measured. CA125 was greater than 35 U/ml in 2.6% of myoma uteri, in 2.4% of benign ovarian tumors and 68.0% of ovarian cancers. Using a cut-off level of 35 U/ml, the results can be characterized by a 68.0% of sensitivity and 97.5% of specificity in detecting an ovarian cancer. Among 25 patients with ovarian cancer, 11 of 14 (78.6%) patients with serous cystadenocarcinoma, 4 of 6 (66.7%) patients with mucinous cystadenocarcinoma and 2 of 5 (40.0%) patients with endometrioid carcinoma had CA125 values greater than 35 U/ml. In relation to a higher tumor stage, there was an increasing incidence of elevated levels of CA125. As a result, it is likely that the measurement of CA125 is useful for early diagnosis of ovarian cancer.

선인장 양심실 보조장치의 설계 및 임상적용을 위한 평가

민병구,박찬영,최재순,이혁수,황창모,김삼성,윤걸중,김종원,선경,이경갑,정종태,김원곤 제주대학교 인공심장이식연구소 2001 인공심장 연구 Vol.2 No.1

현재 개발되고 있는 맥동형 이식형 인공심장은 완전이식형 인공심장과 좌심실보조장치 뿐이다. 좌심실보조장치를 장착한 환자의 10~15%가 우심실의 보조를 필요로하고 완전이식형 인공심장의 장착을 위해서는 자연심장을 제거해야하는 상황에서 이식형 양심실보조장치에 대한 필요성이 대두되고 있다. 본 연구진은 완전이식형 인공심장으로 개발된 한국형 인공심장을 개선하여 양심 실 보조장치를 개발하였다. 양심실보조장치는 이동형 작동기식 미케니즘을 이용하고 있으며 에너지 변환장치와 감속기로 구성된 작동기와 혈액주머니, 그리고 내장형 제어기로 구성되어 있다. 선인장 펌프로 명명된 KAH350은 캐뉼라의 연결을 위한 커넥터를 포함하여 길이 177mm, 폭 164mm, 높이 67 이고 무게 780g이며 최대 심박출량은 5L/min 이다. 양심실보조장치로 개발된 선인장펌프는 좌심실보조장치로도 응용이 가능하다. 이식적합성과 생체적합성을 평가하기 위하여 좌심실보조장치로 5회, 양싱실보조장치로 6회의 동물실험을 수행하였다. 각각 최장 28일간 생존하였으며 11회의 동물실험중 장치의 결함에 의한 것이 3회 있었는데 이들은 모두 전자장치의 결함과 방수처리문제였다. 현재 이식적합성과 일박출량을 향상시킨 KAH400모델이 개발중이다. The types of pulsatile implantable artificial hearts that are on the way of development and have been developed are totally implantable artificial heart and implantable left ventricular assist device (LVAD). Approximately 10% to 15% of all patients Implanted with wearable VADs have required right heart support with another device. And it is reluctant to patient who should remove his or her own heart to be implanted with total artificial heart. These situations drive the development of implantable bi-ventricular assist device (BVAD). The Korean BVAD was develophed by modifying the moving actuator type Korean artificial heart. This electro-mechanical BVAD comprises actuator including energy converter and reduction gear train, blood sacs, and internal motor and energy controllers. The KAH350 which is named as 'Cactus Pump' is 177mm in length, 164mm in width, and 67mm in thickness including connectors and nuts. The weight and maximum cardiac output of Cactus Pump is 780g and 5L/min, respectively. The Cactus Pump that was developed as BVAD could be used as LVAD by attaching compliance caps on the 2 ports of one ventricle. The animal experiments were undergone 5 times for LVAD, and 6 times for BVAD. The best records were 28 days survival in both applications. There were 3 times of device failure and they were all associated with electrical connection and hermetic sealing. The KAH400 that has improved anatomical fitting characterisitcs and stroke volume is on development.

주요우울증에 대한 벤라팍신의 효과 및 안전성

이민수,남종원,강성민,연병길,오병훈,이철,정인과,채정민,백인호 대한신경정신의학회 2000 신경정신의학 Vol.39 No.6

연구목적 : 주요우울증 환자에서 벤라팍신의 치료 효과와 안정성을 조사하기 위해 다기관 개방연구를 시행하였다. 방 법 : DSM-IV 진단 기준에 의거하여 주요우울증으로 진단된 환자중 벤라팍신 투여에 동의한 환자를 대상으로 하였다. 선택된 환자에서 다른 항우울제를 복용하고 있는 경우 14일 이상의 약물 배설기간을 가진후 벤라팍신을 6주간 투여하였으며, 기준점, 1주, 2주, 4주, 6주 후에 HAM-D, MADRS, 그리고 CGI등을 사용하여 평가하였다. 본 연구기간중 발생된 모든 부작용은 부작용의 발생 및 소설 시기, 심한 정도, 발생 빈도, 벤라팍신과의 관련성, 관련 조치 및 결과에 대하여 증례기록서에 기록하도록 하였다. 결 과 : 총 141명을 대상으로 하였으며, 이중 94명(66.7%)이 6주간의 연구를 완결하였고, 47명(33.3%)이 중도탈락하였다. HAM-D총점수는 벤라팍신 투여 1주후부터 유의한 감소를 나타내었으며, 2주,4주, 6주후에도 지속적인 감소를 보였다. MADRS의 총점수도벤라팍신 투여 1주 후부터 유의한 호전을 나타내었으며, 2주, 4주, 6주 후에도 지속적인 감소를 보였다. CGI에서도 기준점, 1주, 2주, 4주, 6주 후 각각의 시점 사이에 유의한 호전이 보였다. 활력징후, 일반 혈액학, 생화학, 심전도 검사 등에서 유의한 변화는 없었다. 벤라팍신과 관련된 부작용으로 흔히 보고한 증상은 오심(10.6%), 소화불량(9.5%), 변비(8.5%), 현기증(8.5%)등 이었다. 결 론 : 다기관 개방연구를 통해 주요우울증 환자에 대한 벤라팍신의 투여는 우울증상의 호전에 효과적이었으며 안전하였다. Objective : A Multicenter open-label study was conducted to evaluate the clinical efficacy and safety of venlafaxine for the tretment in patients with major depression. Method : The study was done in patients with major depression diagnosed by DSM-IV who accepted venlafaxine medication. In cases of the patients taking other antidepressants, 6 weeks of venlafaxine medication was carried out after 14 days of drug excretion period and evaluation using HAM-D, MADRS, and CGI was done at baseline, and after 1, 2, 4, and 6 weeks. Regarding all side effects that had occurred during the period of our study such as their developed/disappeared time, severities, incidences, relationship with venlafaxine, managements and results have been putted into the records. Results : A total of 141 patients were enrolled. Among 94 of them finished the 6 weeks of research and 41 of them did not make it through the research. Total HAM-D scores showed significant decrease after 1 week of venlafzxine medication and continous decrease through the study period. Total scores of MADRS also showed significant improvement after 1 week and continuous decrease through the study period. Similarly, CGI showed significant improvement between baseline, 1, 2, 4, and 6 weeks. There were no significant changes in vital sign, CBC, chemistry, and EKG. The commonly reported side effects of venlafaxine were nausea(10.6%), indigestion(9.5%), constipation(8.5%), and dizziness(8.5%). Conclusion : According to the results, venlafaxine was effective and safe in the treatment of patients with major depression.

스포츠 에어로빅시 High Kick 동작의 운동학적 분석

우병훈,인희교,이수재 한국운동역학회 2001 한국운동역학회지 Vol.11 No.3

This study serves the purpose of understanding the principles of kicking moves through kinematics analysis on elite sports aerobic athletes making the movement of high kicking. The study also intends to not only help instructors and coaches understand the accurate move of high kick and make a better judgement for athletes but also lay a basic resource for them to rely on. To accomplish all this, the time span of the each phase, the displacement of COG, the velocity of left/right forefoot, the angle displacement of left/right hip joint and the angle displacement of left/right ankle joint have been studied. The conclusions were as follows; 1. It took less time at phase 3(0.40sec) and phase 5(0.40sec) of returning the leg than at phase 2(0.56sec) and phase 4(0.43sec) of kicking up. 2. The displacement of COG was low at phase 1, 3, 5(103.02±5.70, 95.33±2.45, 96.66±3.20 cm) and high at phase 2(111.18±9.97) and phase 4 (111.61±5.62 cm). 3. About the velocity of left/right forefoot, kicking up the right foot was faster than returning it at phase 1-2(1081.63±40.62an/sec) and phase 2-3(992.92±45.68 cm/sec). With the left foot, kicking up was faster than returning at phase 3-4(1116.25±63.46cm/sec) and phase 4-5(1043.63±40.62cm/ sec). 4. The left and right angle displacement of hip joint showed the maximum extension at phase 1(174.4±52.36, 162.6±05.40deg/sec), phase 3 (170.66±5.94, 165.89±4.36deg/sec), phase 5(166.18±4.83, 157.05±3.59deg/sec), phase 2(26.19±5.40deg/sec) showed the minimum angle of the right angle and phase 4(27.67±5.31deg/sec) showed the minimum of the left angle. 5. In the left and right angle displacement of knee joint, the angle displacement on the right knee joint showed the maximum extension at phase 1, 2, 3, 4(169.10±2.96, 169.91±6.20, 153.14±6.32, 162.11deg/sec) and flexion at phase 3(140.45±10.37deg/sec). The left knee joint showed the maximum extension at phase 1, 3, 4, 5(169.15±4.25, 157.99±10.82, 172.14±3.06, 168.72deg/sec) and flexion at phase 2(148.17±9.64deg/sec). 6. The angle displacement on the right ankle joint showed flexion at phase 1, 3, 4, 5(125.15±4.81, 101.66+2.80, 102.88±8.89, 106.27±5.76deg/sec) and the maximum extension at phase 2(152.22±5.18deg/sec) The left ankle joint showed flexion at phase 1, 2, 3, 5(104.90±48.10, 116.06±15.84, 106.55±8.84, 118.79±6.89deg/sec) and showed the maximum extension at phase 4(153.57 ±4.65deg/ sec).

마이크로 컴퓨터를 이용한 차량의 엔진점화시기 제어에 관한 기초 연구

전병실,김영수 全北大學校 1985 論文集 Vol.27 No.-

A system using ZDS, to control the ignition advance angle according to engine speeds, is designed and tested. The following conclusion is obtained by its experimental results; 1. Microcomputer aided advance angle control is successfully performed(according to engine speeds and usual advance angle characteristics). 2. It is possible to design the microcomputer-aided control system on one board and accordingly to install it on domestic automobiles. 3. The developed software can be put in ROM and alternated if necessary. Further study is desirable to be followed in order to develop other systems able to control any other parameters as well as ignition advance angle of domestic automobile engines.

半夏의 修治方法에 따른 細胞毒性硏究

李秀培,金德宗,金康柱,田炳勳,禹元洪 圓光大學校 韓醫學硏究所 1993 원광한의학 Vol.3 No.1

The purpose of this study is to know the effects of Pinelliae Tuber on the cytotoxicity of human gingival fibroblasts according to brewing methods. Pinelliae Tuber was extracted by water in the state of being raw materials, prebrewing with Zingiberis Tuber and alumen. The extracted Pinelliae Tuber was applied to the cultured human gingival fibroblasts. The cytotoxic effct of Pinelliae Tuber was meaeured by MTTassay and microscopic findings. The results were as follows : 1. The cell viability of the fibroblasts by Pinelliae Tuber water extracts significantly decreased compared to control group. 2. The cell viability by the Pinelliae Tuber extracts prebrewed with Zingiberis Tuber was similiar to those of control group. 3. The cell viability by the Pinelliae Tuber extracts prebrewed with alumen slightly decreased, but was higher than those of PT group. 4. According to the microscopec findings, the number of cell count were smaller than normal in all experimental group, specially in the PT group, and showed the shrinkage of cytoplasmic process in the PT group and PA group.

增損五積丸 腎積方이 腫瘍細胞에 미치는 影響

金質洙,田炳薰,禹元洪,鄭遇悅 圓光大學校 韓醫學硏究所 1994 원광한의학 Vol.4 No.1

The anticancer effect of Geungsonojekhwaon-Sinjekbang(增損五積丸 腎積方) was evaluated on human cancer cell lines (A549, HCT-15, Caki-1) after exposure to Geungsonojekhwaon-Sinjekbang water extract 0.1㎎/㎖, 0.2㎎/㎖, 0.4㎎/㎖, 0.8㎎/㎖, 1.6㎎/㎖, 3.2㎎/㎖, 6.4㎎/㎖, for 1,2,4 hours at 37℃, 5% Co_2 using in vitro agarmethy1-cellulose clonogenic assay, and for 24,48,72 hours in sulforhodamine B assay, which was regarded as a valuable method for cytotoxic effect of unknown compound on cancer cell lines. That is very rapid and expensive method. The aim of the present studies was to the inhibitory effect of Geungsonojekhwaon-Sinjekbang on the growth of human cancer cell lines. From this assay Caki-1 was considered to be a little sensitive to Geungsonojekhwaon-Sinjekbang water extract. The results were obtained as follows. 1. Good corelations were shown from the results of SRB assay and those of clonogenic assay. 2. After the exposure to Geungsonojekhwaon-Sinjekbang water extract, the proliferation of A549, HCT-15, Caki-1 were slightly inhibited. 3. As a result of the exposure to Geungsonojekhwaon-Sinjekbang water extract, RJB extract showed more effective response of inhibitory proliferation of Caki-1 cell lines than that of other cell lines. 4. The exposure to Geungsonojekhwaon-Sinjekbang water extracted in 40℃ water showed more effective cytotoxic response to cancer cell lines. 5. The results showed that the higher concentration the more effective in the inhibition of proliferation of cancer cell lines, especially the cytotoxic effect of 1.60mg/ml and 3.20mg/ml of Geungsonojekhwaon-Sinjekbang concentration appeared to be the most effective concentration according to the increase of concentration.