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肝吸蟲症에 있어서 間接螢光抗體反應(IFAT)을 이용한 血淸 및 濾紙吸着血液의 抗體價에 관한 比較硏究
趙秉洙,嚴基善,林漢鍾 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.3
The indirect fluorescent antibody test (IFAT) for clonorchiasis was performed with sectioned adult worm antigen of Clonorchis sinensis using sera and blood collected on filter paper. Total 277 sera were tested which were obtained from 171 confirmed cases of clonorchiasis, 33 cases of parasitic infection other than Clonorclzis sinensis and 70 cases of healthy individuals from endemic or nonendemic areas of clonorchiasis. The serum IgG titer 1:16 or over was regarded as positive. Thirty one out of thirty three (93.9%) sera from persons with parasitic infections other than Clonorchis sinensis were negative. Serum IgG titers of 70 healthy controls showed 1:8 or below. IFAT of clonorchiasis sera with sectioned antigen of adult worm showed specific fluorescence on the tegumental syncytiuni and cecal epithelium most significantly in positive sera, but negatively counter stained in negative sera. The use of the blood collected on filter paper showed simiiar results. Comparison of the sensitivity between the filter paper technique and the method using sera showed similar positive rates by IFAT. 142 out of 174 (81.6%) clonorchiasis sera were positive when the blood collected on filter paper were used; 140 out of 174 (80.3%) were positive when sera were used. The titers (log₂ units) of the four classified groups increased with the intensity of the infection by E.P.G.: 4.12 in light infection, 4.43 in moderate infection, 5.00 in heavy infection and 6.30 in very infection group. The relation of the titers (log₂ units) between the blood collected on filter paper(y) and sera(x) made the regression equation: y= 0.4x+1.85. showing relatively high correlation(r= 0.60. n = 52. p < 0.01).
김선희,이병대,김세정,이철희 嶺南大學校 工業技術硏究所 1995 工業技術硏究所論文集 Vol.23 No.1
This study was aimed to investigate treatment feasibility of leachate from landfill that was located in Bancheung-Dong Kyungbuk Talsung-Kun. From the analytical results of leachate, high concentration of organic and nonbiodegradable matters was contained. It could not be treated by biological process only thus required a combined treatment able to high rate COD removal efficiency. Two types of Fenton's oxidation were adapted in this study. The first one is pre treatment before biological treatment. The second one is post treatment after biological treatment. Thus, the optimal conditions in both methods were investigated. From the survey of treatment conditions, in case of pre treatment, the optimal condition was ?? 0.1, ?? 27.08, pH 3, temperature 30℃ and reaction time 2 hr. On the other hand, ?? 0.14, ?? 57.42, pH 3, temperature 30℃ and reaction time 1.25 hr was obtained for post treatment as optimal conditions. In the above optimal conditions, high COD removal efficiency was shown in pre and post treatment. Also it can be expected that nonbiodegradable matters are degraded and converted to biodegradable matters.
韓秉基,崔善雄,李位魯 弘益大學校 科學技術硏究所 2005 科學技術硏究論文集 Vol.16 No.-
Full-beads of multi-layer steel engine head gaskets that are used to seal the combustion gas between the head and the block are subject to cyclic bending stresses due to the variation of the head/block gap during engine operation. The L-D curve of the full-bead formed on SUS304 thin plate is important for the sealing capacity assessment. Therefore the experimental method for measuring the L-D curve must be established in gasket company. In this study the rig and the data acquisition system for measuring the L-D curve is suggested. And the experimental results are shown. This results are compared with numerical rsults obtained by using ABAQUS package.
남해선,황규윤,,백병준,정윤영,오천환,조성란,박준수 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1
To evaluate usefulness of application of MAST-CLA to indetify allergens, authors conducted MAST-CLA allergen-specific IgE assay and collected allergy related symptoms in 118 allergic patients diagnosed at Soonchunhyang University Chunan Hospital during October 1998 through January 2000. The results were as followings; 1. The mean (SD) age was 23.1 (±12.17) years old and teenagers accounted for 33.1% of patients. 2. The frequent allergic symptoms were sneezing (59.3%), rhinorrhea (53.4%), nasal stiffness (44.1%), itching sensation (24.6%), cough or sputum (16.1%), and eye symptom (8.5%) in order. 3. Among these subjects, 62.7% of patients revealed at least more than one specific allergen and 70.3% showed elevated total IgE levels by MAST-CLA. The three most frequent specific allergens were Dermatophagoides farinae (47.5%), Dermatophagoides pteronyssinus (45.8%), and house dust (30.5%). 4. While age was not significantly related to positive of both specific allergens and total IgE (P>0.05), sneezing and nasal stiffness were significantly associated with positive of specific allergen and total IgE, respectively (both P<0.05). 5. Total IgE increased with the number of specific allergens, but both specific allergen and total IgE were not significantly related to increases of allergic symptoms (P>0.05). In conclusion, our data suggest that MAST-CLA allergen-specific IgE assay is useful measure for identification of causative allergens in allergic patients, however, it is recommended that more specific allergic antigens should be determined and replaced or added to Korean inhalent panel of MAST-CLA.
Molecular Typing of Pseudomonas aeruginosa by Randomly Amplified Polymorphic DNA
양병선 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.4
Pseudomonas aerugionsa is a commonly isolated nosocomial pathogen. DNA fingerprinting of P. aerugionsa is examined by randomly amplified polymorphic DNA (RAPD). In this study, P. aeruginosa were isolated from environmental and clinical specimens and the molecular typing of the microorganisms was investigated by RAPD. Thirty strains of P. aeruginosa were selected from the strains isolated formerly and submitted for type identification to the University Hospital. 15 strains of P. aeruginosa were received from Chungnam University Hospital and 14 strains from Gyeongsang University Hospital. DNA of P. aeruginosa was extracted by Qiagen genomic DNA kit. PCR mixtures were set up and incubated. Reactions mixtures were made to be optimal for P. aeruginosa. RAPD typing analysis was carried out by the multivariate statistical program (MVSP) V3.0. RAPD type I was the most common pattern and included 23 strains. Most of strains from Gyeongsang University Hospital belonged to RAPD type Ib and 15 strains from Chungnam University Hospital to RAPD type Ⅰ or Ⅱ. RAPD typing of P. aeruginosa isolated from the environmental and clinical specimens was very simple and reproducible.
Molecular Epidemiology of Listeria monocytogenes by Ribotyping
양병선 대한의생명과학회 2002 Biomedical Science Letters Vol.8 No.2
Ten Listeria monocytogenes were isolated from clinical specimens and mussels, and their physio-biochemical characters were compared with the type strains. Ribotyping was used as a taxonomic tool to determine molecular epidemiological marker. Chromosomal DNA was cleaved with restriction enzymes HindIII and EcoRI. The fragment were subjected to Southern blot hybridization with 16S rDNA from B. subtilis by PCR. EcoRI patterns of Listeria strains showed 6 to 8 bands ranging from 0.75 kb to 11 kb band and they were classified into 6 groups. In comparison, HindIII patterns revealed that 5 to 7 bands ranging from 2.75 kb to 7.75 kb band and they classified into 5 groups. The various patterns of Listeria strains were observed within genus, species and isolated sources. 16S rRNA gene restriction patterns (ribotyping) are useful in epidemiological and taxonomic study.