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藥鍼用蜂毒液이 黑色腫細胞에 미치는 抗癌效果에 對한 分子生物學的 硏究
朴贊烈,南相水,金昌煥,李栽東,姜成吉,李潤浩,安秉哲 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-
To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability, apoptosis, and cell cycle were analyzed using MTT assay, tryphan blue assay, [3H]thymidnine release assay, flow cytometric analysis, activity of caspase-3 protease activity assay, and immunocytometric analysis of PCNA. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis- and cell cycle-related genes was performed. The obtained results are summarized as follows: 1.The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2.Significant induction of apoptosis was identified using tryphan blue assay. [³H]thymidine release assay, and flow cytometric analysis of sub G₁fraction. 3.In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4.Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-X□ were down-regulated whereas Bax was up-regulated by bee venom treatment. 5.In flow cytometric analysis of cell cycle and immunocytometric analysis of PCNA expression, cell numbers of G₁phase was increased by a dose-dependant manner. 6.In quantitative RT-PCR analysis of the cell cycle-related genes, p21, p27, and p57 were increased, while Cyclin D1, CDK4, c-Myc, c-Fos, and Histone H3 were decreased. In contrast, there were no remarkable changes in expression levels of CDC2 and c-Jun.
이병찬,김승호,김정우 한국의사결정학회 1998 경영과학연구 Vol.7 No.-
The objective of this study is to examine the influence factors for successive performance of ISO 9000. Especially we focus to propelling motives and quality requirements in certifying ISO 9000. The data for empirical test was gathered from the obtaining firms of ISO 9000 quality certification among electronic and electric manufacturing industry. The results of empirical study suggested that there is significant positive impact competitive advantage motive to quality requirement enforcement. And we found that quality management system establishment, employee's perception and behavior change about quality were impacted very strong to competitive advantage motive and quality improvement requirement enforcement. But competition improvement was impacted to customer claim motive very strong. Also we investigated the problems in certification obtaining progress, post-operational management, and the ISO regulation & system. The result appeared very important factors to overloading of worker in certification obtaining progress, less participation of company-wide employee in post-operational management, and much maintaining cost in the ISO regulation & system.
李秉讚 대진대학교 1996 大眞論叢 Vol.4 No.-
This study is the work that I have researched the plot on <NAMWONGOSA>, one of the representative another volumes of the <CHUNHYANGJUN>. The characteristic of this novel is the longest story among the <CHUNHYANGJUN> that were written before 20th century and is composed of more than about one hundred thousand words. It's plot is largely divided into the former part and the latter. The former part is the first meeting, the true love, and the parting course. The latter is expressed with the course in which the two characters must overcome the trial of their loves and relieve the pain each other. They are destined to meet the desperate love in the former part, but as a resolt they have to achieve their profound love with their happy ending.
박희찬,강효경,김병찬,박성수 釜山大學校生産技術硏究所 1999 生産技術硏究所論文集 Vol.56 No.-
카올린으로부터 미립 일루미나 분말을 합성하였다. 황산용액을 이용하여 액상침전법으로 알루미나를 추출하였다. 알루미나 추출에 미치는 황산농도, 반응온도 및 반응시간의 영향을 조사하였다. 이 과정에서 수화 황산알루미늄이 용출액으로부터 석출되었다. 이때 석출제로는 에탄올을 사용하였다. 이 석출물을 가영하여 미립 알루미나 분말을 얻을 수 있었다. DTA, SEM, TEM 및 XRD를 이용하여 분말의 특성을 조사하였다. Fine alumina was synthesized from kaolin. The alumina was prepared by liquid phase precipitation method using H?SO? solution. The effects of acid concentration, reaction temperature and reaction time on alumina extraction were investigated. Using an ethanol as precipitating agent, hydrated aluminum sulfate was precipitated from leach liquor. Fine alumina powder was obtained by calcining the precipitate. DTA, SEM, TEM and XRD were employed to characterize the powder.
鳳首山 억새(Miscanthus sinensis) 草地의 生育特性과 生産性 관한 硏究
姜炳燦 公州大學校 資源科學硏究所 1993 資源科學硏究論文集 Vol.1 No.-
This study is performed to find the effects of environmental factors on the seasonal changes of the matter production and the characteristic growth of Miscanthus sinensis grassland of Mt. Bongsu in Yesan. Investigation was carried out from the middle of April till mid-October of 1993. The results obtained form this study can be summarized as follows: 1. The peak value of standing crop of above-ground parts was 2,435. D.Mg/m²and that of under-ground psrts was 849.8D.Mg/m²on October 15. 2. The peak productivity of above ground-parts was 30.32 D.Mg/m²/day from July to August and that of under-ground parts was 3.63 D.M.g/m²/day from August to September. 3. The number of leaves per plant rapidly increased from April to August, and gradually decreased thereafter. The LAI was continuously broadened and reached its peak, 5.9m²/m²early in August and then decreased. 4. In a grassland of Miscanthus sinensis, the leaves which were located at the 5th and 6th nodes indicated the maximum photosynthesis. Miscanthus sinensis was from 120 to 140㎝. high. 5. The photosynthetic rate at 50,000 lux and 30℃ was not achieved under the condition of CO₂concentration in the atmosphere. 6. The optimum temperature of photosynthetic activity ranged from 30℃ to 35.℃
趙炳贊 동국대학교 1985 論文集 Vol.24 No.-
With the economic development, it has become a universal trend that floriculture is one of the necessities of life. Accordingly, the demand for floriculture in Korea increases rapidly since 1970 with the rapid economic development, though, in 1975, per capita consumption of floriculture in Korea was 5% of Japan, 1.25% of America and less than 1% of Germany. However, in 1970s, the rate of increase of the demand per household in Korea is 700%, higher than that of Japan, 520%. It is true that floriculture industry in Korea has made a remarkable development due to the increase of the demand. Recently, Korea imports a lot of flowers as the domestic supply has been unable to meet inflated demands. As a result, Korea is a chronic crop importing country including floriculture, with a widening adverse balance of trade. It is urgently needed that Korea should grow and develop its floriculture as an essential industry. The problems of floriculture in Korea, in spite of the rapid development in 1970s, are: the poor capital and facilities due to the pettiness of the individual farmers, the decrease of the ratio of the big-scale farmers and increase of the petty farmers, the regional concentration and disparity in technique of cultivation due to the lack of recognition among the general people and diffusion of technique, the pre-modern market dominated by the oligarchic merchants. Thus, the following steps are necessary for the healthy growth and development of floriculture in Korea. 1. the general policies with new recognition and efforts for the development of floriculture, 2. the positive aid of fund and facilities to solve the pettiness of the farmers, 3. the establishment of the modern flower whole-sale markets to protect the farmers from the arbitrary exploitation by the pre-modern oligarchic merchants, 4. the organization of the floricultural farmers for direct and joint sale, 5. the development and equalization of the technique for the cultivation and merchandising of the floriculture, 6. the positive development of the demand of floriculture for the people's emotional refinement and labor efficiency through th environmental beautification, 7. the exploitation of the oversea markets.
小賣市場에 있어서의 「단골去來關係」에 관한 調査硏究 : 生活必需品을 中心으로 in case of the main necessaries of life
趙炳贊 東國大學校 1977 論文集 Vol.16 No.-
This survey was conducted among 332 housewives throughout Seoul for a month (June 15, 1977-July 15, 1977). The survey is to examine the fixed relationship of customer and retailer in the retail market of the necessaries of life. The contents and result of the survey are as follws. Most items of the necessaries of life are purchased mainly by housewives who show the strong tendency to have the fixed retail shops in each item. The rate of the fixed shop by the items is; fuel 88.0% food 59.2% garment 50.3%. The itmized rate is; 1. yeontan (coal) 95.2% 2. grain 89.9% 3. kerosen 86.0% 4. gas (L.P.G.) 73.3% 5. meat 70.9% 6. garment (tailored) 64.7% 7. food (others) 54.5% 8. subsidiary food(except meat) 51.8% 9. underwear 50.0% 10. fish 49.0% 11. fruit 39.1% 12. garment (ready-made) 36.2% The housewives usually have one or two fixed retail shops in each item and the dominant factor in the choice of fuel and food is the distance from houses. The most important factor in the choice is "the creditability" and "the lack of kind service" in the avoidance. It is interesting to note that the outward economical inducement such as bargaining in price and quantity is not so important as it was generally believed to be. The important factors in the success of the retail shop, which is measured in the terms of the number of the fixed customers, and the achievement of the larger purposes of (a) rationalization of marketing, (b) maximization of the profit of the retailers, (c) protection of the consumers seems to be (a) creditability (b) kind service (c) list-price system. To achieve the above mentioned purpose, the effort for improvement on the part of the retailers and the proper and appropriate guidance on the part of the big industries and the government agencies concerned as the authority in charge of the marketing are needed. "The Model Retail Shop" must be selected on the basis of "the creditability" and "the kind service", along with the list-price and the issuance of the receipt.
趙炳贊 동국대학교 농림과학연구소 1971 農林科學 論文集 Vol.4 No.-
As generally known, the rural labor force in Korea is characterized by 1) over-employment in the agricultural sector, 2) shortage of manpower in the farming season even under over-employment condition and) lower coefficient of labor utilization. These factors suggest that the agricultural labor in Korea is still in a premordern state. It is to be noticed that in the recent years our agricultural labor force has shown a tendency to decrease due to ever-increasing out-flow to urban communities, causing a severe shortage of manpower in the farming season which affects agricultural productivity in Korea. Keeping the above problems in mind, the author surveyed the rural labor force on a village in the suburb of Seoul, in order to analyze and investigate the aspects of leaving farms and structure of labor utilization. Village surveyed; Village of Yadang-dong, Yadang-ri, Kyoha-myon, Paju-kun, Kyunggi-do. Method of survey; Complete survey of 50 households which comprise the village. Dates of survey; August 1-31, 1970. The results obtained from this survey are summarized as follows; 1. Phenomenon of leaving farms (1) The out-flow of these villagers to urban communities was observed, in general, among the group of the high teens and the first half of forties. The leaving rate is higher in young group. Accordingly, the manpower of this village was old aged; i.e., 34.7% of the age group of 15-24, 32.1% of 25-34 and 22.6% of 35-44 left the village during a period of Jan. 1965-Aug. 1970. (2) The leaving rate is markedly high in the group of higher educational background; i.e.,3.4% of uneducated or under primary school graduates, 20.1% of primary school graduates, 21.7% of secondary school graduates, 42.9% of high school graduates and 88.9% of college or university graduates. (3) In order to analyze the effect of the scale of cultivated area and farm household income on the leaving farms, the correlation between the two factors was investgated, The resultes revealed very low degree of correlation between them. 2. Utilization structure of labor The number of working days per capita in a year and rate of labor consumption (utilization) was 164.0 days and 54.7% for male, 113.7 days and 37.9% for female, and the average of the both sexes was 137.9 days and 46.0%(The percentage is based on 300 working days in a year - 25 days in a month). Such low coefficient of labor utilization may be attributed to the following factors; (1) The seasonal change in the demand of manpower; because of simple cropping method in our country, which depends entirely upon seasonal factors. (2) The fact that most of youth group in the village tend to avoid farming work and to find other jobs because of their inferiority complex with agriculture. Most of them refuse to engage in agriculture even if in the unemployment status. (3) The low participating ratio of labors of both sexes in non-farming work in the farming community; because they are rarely given the opportunity to find and to be trained for a proper job in and around their farm village. 3. Factors determining the labor utilization Upon analyzing, as the determining factors of individual labor utilization, 1) age of laborer, 2) scale of cultivated land, 3) scale of farm household income, it is revealed that the most significant factor is the age of laborer. The age means working capacity of individual farmer and his position or responsibility in the household; i.o., dependent or not. 4. Conclusion (1) The out-flow of the young and educated from this village resulted in; 1) that the average age of the remaining labor is high, 2) that the educational level of the remaining labor is low, causing intellectual vacuum in the village. (2) Utilization of labor The rate of labor utilization in this village is very low, mainly from; 1) the long farmer's slack season caused by the mono-cropping system which depends upon entirely seasonal factor. 2) the difficulties to find proper work (especially non-farming)during the farming slack season. 3) traditional family system of Korea with large number of dependents who can and should work, but whose widely recognized unconscious privilege prevents their positive participation in the farming work.