국소성 분절성 사구체 신병증의 동물 모델 (FGS/kist 생쥐) 신 조직의 유전자 발현 양상

강희경,이병섭,이철호,하일수,정해일,최용,Kang, Hee-Gyung,Lee, Byong-Sop,Lee, Chul-Ho,Ha, Il-Soo,Cheong, Hae-Il,Choi, Yong 대한소아신장학회 2011 Childhood kidney diseases Vol.15 No.1

목 적: 국소성 분절성 사구체 경화증(Focal segmental glomerulosclerosis, 이하 FSGS)은 소아신부전의 원인 중 가장 흔한 사구체 질환이다. 일차성 FSGS의 병인은 아직 알려져 있지 않으므로, 저자들은 FSGS의 동물 모델을 대상으로 cDNA 마이크로어레이를 이용한 유전자 발현 양상 분석을 통하여 유전자 발현 수준에서의 FSGS의 질환의 특성을 밝히고자 하였다. 방 법: 사람의 일차성 FSGS와 유사한 질병경과를 보이는 동물모델인 FGS/kist 생쥐의 신피질 조직을 대조군 생쥐(FGS/kist 생쥐의 조상 strain인 RFM/kist 생쥐)와 AB 1700 mouse chip을 이용한 마이크로어레이 실험으로 비교하였다. 결 과: FGS 질병특이 유전자가 62개 추출되었다. 이들은 세포주기/사멸, 면역반응과 지질 대사/혈관 질환과 관련된 유전자들로써, 유전자간 network의 중심유전자가 면역반응(TNF, IL-6/4, IFNg)과 세포사멸 조절 유전자(TP 53), 그리고 지질대사의 중요 유전자인 PPARG이었다. 결 론: 이 연구에서 저자들은 자발적인 FSGS의 임상경과를 보이는 FGS/Kist 생쥐의 신장조직의 유전자 발현의 분석을 통하여 신장세포사멸과 면역반응에 뒤따르는 기질 섬유화, 그리고 지질 대사의 이상과 조기 혈관 질환이 FSGS의 병태생리에 기여할 것임을 다시 확인할 수 있었다. 추가적인 연구가 계속된다면 global transcriptome profiling 기법으로 병인 탐색 및 치료방법 개발 에 의미 있는 결과를 도출할 수 있을 것이다. Purpose: Focal segmental glomerulosclerosis (FSGS) is the most common glomerulopathy causing pediatric renal failure. Since specific treatment targeting the etiology and pathophysiology of primary FSGS is yet elusive, the authors explored the pathophysiology of FSGS by transcriptome analysis of the disease using an animal model. Methods: FGS/kist strain, a mouse model of primary FSGS, and RFM/kist strain, as control and the parent strain of FGS/kist, were used. Kidney tissues were harvested and isolated renal cortex was used to extract mRNA, which was run on AB 1700 mouse microarray chip after reverse transcription to get the transcriptome profile. Results: Sixty two genes were differentially expressed in FGS/kist kidney tissue compared to the control. Those genes were related to cell cycle/cell death, immune reaction, and lipid metabolism/vasculopathy, and the key molecules of their networks were TNF, IL-6/4, IFN${\gamma}$, TP53, and PPAR${\gamma}$. Conclusion: This study confirmed that renal cell death, immune system activation with subsequent fibrosis, and lipid metabolism-related early vasculopathy were involved in the pathophysiology of FSGS. In addition, the relevance of methodology used in this study, namely transcriptome profiling, and Korean animal model of FGS/kist was validated. Further study would reveal novel pathophysiology of FSGS for new therapeutic targets.

웹 브라우져를 통한 서버 클라이언트 환경의 영상처리 실습 시스템 설계

이병일,손병환,최항묵,최흥국 인제대학교 2001 仁濟論叢 Vol.16 No.1

웹 기반의 환경은 인터넷과 컴퓨터의 접목으로 실생활에서 연구활동에 이르기까지 다양하게 그 효용성의 범위를 굳혀가고 있다. 교육기관에서 활용하는 가상강좌는 이러한 웹의 장점을 활용한 학습방법의 다양화의 결과이다. 본 논문에서 설계한 웹 브라우져를 통한 서버 클라이언트 환경의 영상처리 실습 시스템은 가상강좌나 웹 환경이 구축된 장소에서 교육자와 피교육자사이의 교육효과를 증진시키도록 상호 의사전달의 효율성을 가지게 하는 시스템이다. 각 클라이언트가 다운로드 받은 컴포넌트를 실행하여 구동하게 되면 클라이언트들이 자신의 실습을 위한 프로그램을 로컬에서 가동할 수 있도록 하였기 때문에, 서버측의 부하가 얼어서,실습 결과영상을 로컬 클라이언트에서 부하시간 없이 얻을 수 있게 하였다. 또한 서버의 수정 혹은 갱신으로 바뀌어진 컴포넌트는 클라이언트 측에서 접속시 업데이트가 가능하므로 각 클라이언트에 대한 업 데이트를 따로 고려하지 않아도 되는 장점이 있다. 또한 컴포넌트가 가지는 여러 가지 메쏘드들은 다양한 영상처리의 결과를 볼 수 있게 해 줌으로써 고가의 소프트웨어를 따로 구입하지 않아도 되는 장점을 가지게 된다. The environment of web is applied variously from our life to research activities thanks to the internet and the computer. The virtual lecture utilized by an educational institution is the result of application of a learning system with the advantage of web. In this paper, a design of the image processing training system at the environment of server-client through web browser is the system of high degree of efficiency at the point of communication between lecturer and student. A client can get the result image from another client without load balancing of the server, because the processer for image processing is operated at the container of a client after downloaded as the form of a component. Clients can get the new version of component when they contact, so clients do not need to consider the status of updated component locating in the server. Furthermore, the client can handle an image with the many operations included in component with no purchase of the software additionally, that is another merits of this system.

편측성 구순열 환자의 이차수술후의 비순 폭경의 변화

민병일 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.2

In order to evaluate the changes before and after secondary operations in the patients with cleft lip objectively using full face photographs, the author analyzed results of improvement on nasal width and mouth width and asymmetry of nostril. 1. The ration of asymmetry of thenostril is changed from average 1.24 preoperatively to average 1.08 postoperaively : 61.2% is improved postoperatively. 2. The ratio of nasal width compared with intermedial canthal distance is changed from average 1.15 preoperatively to average 1.10 postoperatively : 3.8% is reduced posteroperatively. 3. The ratio of mouth width compared with intermedial canthal distance is changed from average 1.08 preoperatively to average 1.03 postoperathvely : 4.1% is reduced posteroperatively.

光州川의 水質汚染에 對한 硏究

金秉煥,盧基煥,柳一光 광주보건대학 1985 論文集 Vol.10 No.-

A study on the wate pollution of Kwangju stream by the relationship between physical-chemical condition and indicator planktons distribution by water quality level was examined at 6 sampling positions during the period from Mar, 25 to Jun, 25 1984. The results were as follows; 1. pH was in the range of 6.3∼7.6. 2. The mid-stream of Kwangju stream was the more severly polluted than the other sampling positions and the average range for it was 0.3∼3.9 ppm of DO, 62∼352 ppm of BOD, 5.3∼24 ppm of T-N and 2.3∼140 ppm of T-P. 3. The plankton identification in this period showed 5 genera 6 species of Cyanophycea, 11 genera 15 species of Bacillariophyceae, 9 genera 16 species of Chlorophyceae and 6 genera 7 species of protozoa: total 31 genera 44 species. 4. In biological water analysis, upper stream(sp. 1) showed from Oligosaprobic to β-mesosaprobic but the stream in the mid-town area polysaprobic. 5. In the mid-stream (BOD 62~352 ppm) dominant species were appeared Oscillatoria tennis of Cyanophyceae and Paramecium caudatum and Euglena sp. of Protozoa. 6. We would like to propose establishing sewage plants near to the upper-stream and the down-stream of Kwangju stream.

고래회충유충증 107례 보고 및 어류감염 실태 조사

임경일,신호준,김병화,문승일 INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1995 YONSEI REPORTS ON TROPICAL MEDICINE Vol.26 No.1

고래회충유충증은 바다 포유동물에 기생하는 고래회충과(科)의 유충이 인체의 소화관 특히 위(胃)와 소장에 침입하여 발생되는 질병으로 범세계적으로 보고되고 있다. 급성복통을 호소하는 환자를 대상으로 바닷고기 생식여부를 조사하고 위내시경을 관찰하여 위점막내로 뚫고 들어가는 고래회충과 유충을 적출하여 동정함으로써 진단하였다. 1989년 6월부터 3년간 남자 60명, 여자 47명의 고래회충유충증 환자를 찾아내었다. 주요 증상은 상복부통, 급성복통, 구토 등이었고, 바닷고기를 생식하고 4시간 내지 1일 이내에 증상이 생겼고, 대부분 1-3일 후 유충을 제거하였다. 위내시경 관찰을 하였더니 유충들은 위의 몸체에서 제일 많이 발견되었고 유충이 침입한 위점막으로 부터 출혈, 부종, 미란 등이 관찰되었다. 적출된 유충 90마리 중 82마리가 고래회충(Anisakis simplex)의 유충이었다. 문진을 통해 확인된 환자가 생식한 어종으로는 조기, 바닷장어, 히라스, 오징어가 많았다. 농수산물센타에서 임의 로 구입한 5가지 바닷고기에서 고래회충과 유충을 조사하였더니 고등어와 우럭에서 검출되었고, 내장 및 복부 근육에서 고래회충(Anisakis simplex)과 물개회충(Pseudoterranova decipiens)의 유충 등이 검출되었다. Human anisakiasis may occur after ingestion of raw marine fish infected with nematode larvae of Anisakidae. Anisakiasis caused by the migration of the larva into the wall of stomach, small intestine and other portion has been reported in Korea. This prospective study was made of all cases referred to parasitological laboratory in Cheju-do between June 1989 and June 1992. Gastric anisakiasis was confirmed if larvae invading the gastric wall were in 30-49 years old. Most of the patients complained acute epigastric pain with history of eating raw marine fish. This symptom usually occurred about 12 hours to 1 day after ingestion of infected marine fish. Edema, erosion or ulcer of the mucosa and hemorrhage from the gastric wall were observed in the involved areas. Ninety larvae removed from the stomach were identified; the larva of Anisakis simplex was the most prevalent species, and the larva of Pseudoterranova decipiens was also detected. The important species of marine fish from which the patients became infected was demonstrated as yellow corvina, sea eel, ling, cuttle fish, yellowtail and others. Five species of marine fish as a possible source of infection were examined, and Anisakis simplex larvae and Pseudoterranova decipiens larvae were collected from the mackerel and rock cod. This study demonstrates that anisakiasis is recognized as a public health problem in Korea.

일차 토순 수술후의 코입부위의 심미적 평가 제1보 코의 비대칭성

민병일 大韓顎顔面成形再建外科學會 1994 Maxillofacial Plastic Reconstructive Surgery Vol.16 No.4

The author evaluated the nasal asymmetry after primary operations in the patients with unilateral cleft lip using full face photographs. The results are as follows : 1. Nasal deviation angle is average 2.98+3.01 degree(ranged from 0 to 10 degree), significantly different from control group(p<0.05). 2. Nasal deviation angle is average 5.1% when inter-medial canthal distance is 100%, significantly different from control group(p<0.05). 3. Difference in nostril size between cleft and noncleft side is 2.1% when inter-medial canthal distance is 100%, significantly different from control group(p<0.05). 4. Nasal attractiveness analysis shows higher points in difference in nostril size, nasal deviation, nasal form in that order. 5. Nasal asymmetry after primary operations in the patients with unilateral cleft lip using full face photographs is related with Nasal deviation angle, Nasal deviation distance and Difference in nostril size between cleft and noncleft side.

Pyrantel Pamoate (Combantrin)에 依한 蛔蟲 및 鉤蟲感染의 集團治療

徐丙卨,朴定圭,趙昇烈,姜信榮,魚光本,康世喆,李源宰,李正雨,黃邱一 中央醫學社 1972 中央醫學 Vol.23 No.6

The mass treatment trial of pyrantel pamoate on Ascaris lumbricoides and Ancylostoma duodenale infections was carried out to school-children and inhabitants in rural areas in the central part of Korea. Pyrantel pamoate ("Combantrin") tablet (123 mg base) was administered as a single dose of 10. 0 mg per kg. body weight to a total of 4,198 Ascaris infected cases selected out of 4,436 (41. 6%) positives, who were screened from the total 10,660 collected stool specimens. Of these, only 3,169. cases were followed up after treatment to provide an analysis in this study. Incidentally 161 cases with concomittently infected hookworm were also subjected to assess the efficacy of pyrantel pamoate against A. duodenale. The egg negative conversion rate on A. lumbricoides was 95.3%, and on A. duodenale, 97.5% four weeks following treatment. In failed cases, the egg reduction rates on both of the infections were 97.6% and 97.3% respectively. No side effects and no problem in drug administration were demonstrated. It is concluded that this study confirms the high efficacy of pyrantel pamoate in the treatment of Ascaris lumbricoides and Ancylostoma duodenale infections and also proves the acceptability and safety of this drug in the mass treatment of ascariasis in a population with relatively high worm burden.

베라돈(velardon)의 寄生蟲 驅蟲 效果

朱一,崔秉徹,兪炳秀 醫藥係社 1958 綜合 醫學 Vol.3 No.2

榮山江의 水質汚染에 관한 연구

류일광,이치영,김병환,강영식 광주보건대학 1981 論文集 Vol.6 No.-

Water quality of Yeong San River was examined for 3 months from October 1. 1980 to December 1. 1980. For checking the water quality, 6 sampling positions were selected. The results we obtained are as follows. 1. The average for Yeong San River was 6.9∼7.6 of pH, 0.2∼7.3 ppm of Biochemical oxygen demand (BOD), 9∼236 ppm of Suspended solid (SS), 3.04∼1.59.26. ppm of Total Phosphate, 0.7∼24.62 ppm of Total. Nitrogen. 2. The phytoplankton identification in this survey period showed Cyanophyceae is 7 genera 9 species, Bacillariophyceae 14 genera 25 species and Chlorphyceae 21 genera 26 species: total 42 genera 60 species. 3. The results of biological water analysis were as follows: st. 1 was α msosaprobic and st.2 seemed to be oligosaprobic. St.3 was from α-mesosaprobicto β-mesosaprobic and st. 4 seemed to be β-mesosaprobic. St.5 was from β-mesosaprobic to oligosaprobic and st.6 seemed to be β-mesosaprobic. 4. To appear dominat algea there were Microcyctis, oscillatoria, Anabaena, Melosira, Nitzschia and Synedra.

Pyrantel Pamoate(Combantrin)에 依한 蛔蟲症의 減量療法

徐丙卨,李純炯,趙昇烈,姜信榮,梁龍石,黃邱一,朴定圭,李水月 中央醫學社 1973 中央醫學 Vol.25 No.4

The mass treatment trial with low dosage of pyrantel pamoate against Ascaris lumbricoides infection was conducted to school children in rural areas of Korea. A comparative trial in the use of piperazine salt, santonin-kainic acid complex in the treatment of ascariasis was also attempted to evaluate the efficacy of' pyrantel pamoate. A total of 804 Ascaris infected school children selected out of 822-positives, who were screened from the total 1, 699 collected specimens, were classified into 7 groups. One of the three kinds of anthelmintics, i.e., pyrantel pamoate, piperazine adipate and santonin-kainic acid complex was administered to each group according to the planned regimen. For the evaluation, the egg negative conversion rate(cure rate) and egg reduction rate were calculated 4 weeks after treatment on 623 follow-up cases with the obtained results from the stool examinations (Cellophane Thick Smear Method and Stoll's Egg Counting Technique). The results obtained were; 1. The egg negative conversion rate was the highest in pyrantel treated groups (Group 1, 2, 3), santonin-kainic acid complex treated group (Group 7) coming next, and the lowest in piperazine treated groups (Group 4, 5, 6). 2. The reduction rates were also obtained just as the same order of the above results. 3. Among the pyrantel pamoate treated groups, the low dosage groups (Group 2 8: 3) showed 90.6% and 85.7% of negative conversion rate, and 99. 2% and 98. 2% of reduction rate respectively, while 94.1% of negative conversion rate and 97.9% of reduction rate were obtained in the conventional dosage group (Group 1). 4. On the analysis of efficacy in the groups treated with piperazine adipate, the best results were. shown in 2 or 3 consecutively treated groups (Group 4 &.- 6) under the supervision of qualified doctors, and the worst was in the group treated with 2 consecutive administration by teachers without supervision (Group 5). 5. The side reactions were so negligible in all the trial groups that none of the treated cases complained about the known side effect of those anthelmintics. From the present study, it can be suggested that the pyrantel pamoate is the most effective among three drugs, and also be recommended to apply the low dosage schedule of pyrantel pamoate in the masstreatment of ascariasis to reduce the expense of masstreatment.