Characterization of the European type of maternal lineage evident in extant Jeju native pigs

Byeong‐Woo Kim,In‐Cheol Cho,Moon‐Sung Park,Tao Zhong,임현태,Sung‐Soo Lee,Hee‐Bok Park,Moon‐Suck Ko,Jun‐Heon Lee,Jin‐Tae Jeon 한국유전학회 2011 Genes & Genomics Vol.33 No.2

Using a partial D‐loop sequence of mtDNA, an intensive analysis was conducted of the maternal lineages of Jeju native pigs (JNPs) from Korea. In total, 100 mtDNA sequences were obtained from Asian wild boars (AWBs), European wild boars (EWBs), Asian domestic pigs (ADPs), European domestic pigs (EDPs), and JNPs and were used for phylogeny and network analyses. Two distinct JNP groups - one (JNPA) in the Asian cluster and the other (JNPE) in the European cluster - were identified in the estimated phylogenetic tree and network. The maternal lineage of JNPE was the closest to that of EWB and a clear haplogroup was identified that shared an identical haplotype (hap04) among 15 individuals of JNPE and 2 individuals of EWB. A Landrace and an EWB shared hap03with a JNPE. EWB, Landrace, Large White, and Duroc formed two clear haplogroups with JNPE in a parsimonious medianjoining network analysis, suggesting that an obvious maternal contribution of EDP has occurred in JNPE in recent years. A pair‐wise mismatch analysis also indicated that JNPE may have experienced a sudden population expansion, suggesting a more recent establishment compared with the gradual population expansion of JNPA. The JNPE group therefore should be further evaluated in order to decide whether this group should be culled or accepted into further programs for maintenance of the JNP population as a pure breed.

慶南(경남) 河東(하동)의 儒脈(유맥)과 南冥學(남명학) 傳承(전승)

( Byeong Cheol Jeon ) 경상대학교 남명학연구소 2013 남명학연구 Vol.39 No.-

경남 하동 지역 유학자의 세기별 분포는 15세기 1명, 16세기 2명, 17세기 7명, 18세기 2명, 19세기 16명, 20세기 초 1명이다. 가장 비율이 높은 시기가 19세기이며, 그 다음이 17세기이다. 경남 지역은 南冥 曺植의 학문을 계승한 南冥學派가 주도적 역할을 담당하였는데, 남명학파의 성쇠에 따라 하동 지역의 유학자도 비례하여 산출되고 있는 점을 확인할 수 있다. 구역별 분포는 총 29명의 유학자 가운데 옥종면이 21명으로 가장 많으며, 그 다음으로 북천면이 6명, 금남면과 양보면이 각각 1명이다. 이 가운데 옥종면 안계리 출신의 유학자가 11명으로, 옥종면 유학자의 절반이 넘는 비율을 차지하며, 전체 하동군 유학자의 3분의 1에 해당한다. 이러한 통계를 근거해 볼 때, 하동 유학의 중심지는 옥종면이며, 그 중에서도 안계리가 핵심 지역이라고 규명해 볼 수 있다. 학맥 형성 양상을 살펴본다면, 남명학파 11명, 寒洲系列 4명, 蘆沙系列 5명, 星湖系列 3명, 尤庵系列 1명, 華西系列 1명, 기타 6명이 된다. 그러므로 하동 지역의 학파별 분포는 남명학파 및 南人 계열이 대다수를 차지하며, 그 다음으로 노사계열의 문인이 많은 비중을 가진다. 하동 지역의 남명학 전승과 관련해서는 안계마을 출신의 晉陽河氏 학자들이 家學을 통해 계승한 사실을 거론할 수 있다. 그리고 河達弘, 鄭瑀贇, 趙性家, 崔琡民, 姜柄周, 鄭敦均 등의 학자들이 서로 다른 학맥과 당파를 가지고 있었음에도 불구하고, 남명을 존숭하고 본받아 실천하려 한 점을 지적할 수 있다. According to the distribution of Confucian scholars in Hadong (河東)-gun, Gyeongnam (慶南) by century, the 15th century has one, the 16th century has two, the 17th century has 7, the 18th century has two, the 19th century has 16, and the early 20th century has one. The period that indicates the highest rate is the 19th century, and the next is the 17th century. In Gyeongnam area, Nammyeong (南冥) School that transmits Nammyeong Jo Sik’s studies has played the initiative roles. And we can see that Confucian scholars in Hadong area are produced proportionally to Nammyeong School’s rise and decline. About their distribution by area, Okjong-myeon has produced the most Confucian scholars, that is, 21 out of total 29. The next is Bukcheon-myeon as six, and Geumnam-myeon and Yangbo-myeon have produced one each. Among these, Confucian scholars from Angye-ri, Okjong-myeon are 11, so they are more than half of the Confucian scholars from Okjong-myeon. And they take up one third of the entire Confucian scholars from Hadong-gun. Based on the statistic data, we can establish that the center of Hadong Confucianism is Okjong-myeon, and in that area, Angye-ri has formed the core area. According to the aspects of formation of academic lines, Nammyeong School has 11, Hanju (寒洲) Affiliation has four, Nosa (蘆沙) Affiliation has five, Seongho (星湖) Affiliation has three, Wooam (尤庵) Affiliation has one, Hwaseo (華西) Affiliation has one, and the rest are six. Thus, according to the distribution of schools in Hadong-gun, Nammyeong School and Namin lines occupy the majority, and the next highest are literary men of Nosa Affiliation. About the transmission of Nammyeong studies in Hadong area, we can come up with the fact that Jinyanghassi Sajikgongpa (晉陽河氏 司直公派) scholars from Angye Village did transmit it by family learning. And we can also point out that scholars like Ha Dal-hong, Jeong Woo-bin, Jo Seong-ga, Choi Sook-min, Gang Byeong-ju, or Jeong Don-gyun did respect and emulate Nammyeong and tried to practice it even though they were from different academic lines or factions.

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

정신분열증 환자의 혈청내 Haloperidol과 Prolactin 함량에 관한 연구

연병길,한규희,양병환,김철규,전남호 漢陽大學校 精神健康硏究 1985 精神健康硏究 Vol.3 No.-

소아의 치아와 지지조직 외상에 관한 분석

백병주,양연미,양철희,김재곤 大韓小兒齒科學會 2001 大韓小兒齒科學會誌 Vol.28 No.4

본 연구는 소아의 외상에 관한 실태를 파악하여 외상의 예방 및 치료법을 확립하고자 전북 대학교병원 소아치과에 1998년 4월 1일부터 2000년 3월 31일까지 2년간 외상을 주소로 내원한 212명(치아수:431개)의 환자를 대상으로 설문지 및 구강검사를 통하여 연령, 성별분포, 발생원인, 초진시 처치, 치아외상의 세부항목을 조사, 분류하여 다음과 같은 결론을 얻었다. 첫째, 모든 나이군을 통해, 남아가 여아보다 치아외상의 더 높은 빈도를 보인다. 둘째, 환자방문이 가장 높은 나이는 유치에서는 1-2세, 영구치에서는 8-9세이다. 셋째, 외상원인으로 유치는 넘어짐, 추락, 충돌이고, 영구치는 넘어짐, 충돌, 사고, 타박 등이다. 외상빈도는 계절적으로 유치는 5월, 영구치는 3월에 가장 많으며, 유치에서는 수요일에, 영구치는 화요일과 목요일에 많다. 치아외상은 주로 오전에 발생한다. 넷째, 어린아동들은 넘어지거나 가구에 부딪혀서 가정에서 주로 손상을 받는다. 나이든 아동들은 가정밖인 학교등에서 스포츠나 사고등에 의해 외상이 증가한다. 다섯째, 가장 흔히 손상받는 부위는 상악유중절치, 상악중절치이다. 유치에서 손상은 동요·진탕이 많고, 영구치는 치관파절이 많고 또한 동요·진탕도 많다. 여섯째, 상하순 및 상악치은 열상이 가장 흔한 연조직 손상으로 나타난다. 일곱째, 초진시 처치는 유치에서 경과관찰과 발치가 가장 흔한 치료법이며, 영구치에서는 주로 수복처치와 정복고정이 시행된다. The purpose of this study is concerned wit an investigation of the actual condition of traumatic injuries of teeth and supporting structures in children to set up possible criteria for prevention and treatment of injured teeth. The materials consisted of the clinical records of 431 traumatically injured teeth of 212 children, accumulated during two years from the first of April in 1998 to the end of March in 2000, supplied from the Dept. of Pediatric Dentistry, Chonbuk National University Hospital. The incidence of injuries in boys was extremely high for permanent teeth, twice as high as in girls. Accidents to primary teeth were most common from 1 to 2 years of age and to permanent teeth were most from 8 to 9 years of age. The most common cause of trauma was a fall for both primary and permanent teeth, followed by collision. The anterior teeth in maxilla was most frequently affected by trauma in both the primary and permanent teeth. The most common type of trauma were loosening for the primary teeth, followed by luxation types which included the intrusion, displacement and extrusion and complete avulsion types. For the permanent teeth, the most common type of trauma were tooth fracture. The most common trauma of soft tissue was laceration of upper lip, lower lip and gingiva of maxilla Concerning treatment at the first visit, primary teeth with only loosening and concussion were not usually treated. Permanent teeth were often treated by crown restorations for crown fractures and by endodontic procedures for pulpal exposure. Though we could elucidate actual condition of traumatic injuries of teeth in children, we should make a follow-up survey to ensure the prognosis of injured teeth and establish the most desirable criteria for traumatized teeth in children.

정상, 낭종 및 법랑아세포종 세포에서의 유전자 발현 차이 분석

양철희,김재곤,백병주,양연미 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.1

법랑아세포종은 1868년에 처음 보고된 이래 명칭, 발생기전, 분류 그리고 치료 방법 등에 관하여 수 많은 논란이 있어 왔는데 이는 법랑아세포종이 양성종양임에도 불구하고 종양자체의 진행이 파괴적이고, 외과적 처치를 한 후에도 재발이 잘되며, 흔하지는 않지만 악성종양과 유사하게 전이를 보이는 등 독특한 특성을 지니고 있기 때문이다. 정상세포와 암 세포 간에 차이를 보이는 유전자 혹은 정상세포에서 변형이 일어날 때 특이적으로 발현하는 유전자의 분리 및 분석하는 것은 암세포의 생성과정을 이해하는데 있어서 중요한 열쇠를 제공할 수 있다. 이에 본연구는 RNA differential display 방법 중 재연성과 반복성이 개선된 Ordered differential display(ODD)RT-PCR과 보다 개선된 GeneFishing™기술을 이용하여 악성과 양성종양 사이의 유전자 발현의 차이를 조사하고, 특이 유전자의 profile을 확보하고자 하였다. GeneFishing™기술과 RT-PCR을 수행한 결과 nasopharyngeal carcinoma gene을 제외한 9개의 유전자는 악성에서 특이적으로 발현되는 것을 확인하였다. 따라서 GeneFishing™을 이용하면 각 시료간의 mRNA 상에서 발현차이를 보이는 DEG를 비교 분석하면 암관련 유전자, 항생제 내성 유전자, 그리고 분화 관련 유전자들에 대한 연구가 용이하게 수행할 수 있을 것으로 생각된다. Ameloblastoma is the most commonly occurring odontogenic tumor in oral cavity. Although most are benign epithelial neoplasm, they are generally considered to be locally aggressive and destructive, exhibiting a high rate of recurrence. The biological behavior of this neoplasm is a slowly growing, locally invasive tumor without metastasis, therefore malignant neoplasm, changed its histological appearance to carcinoma or showed distant metastasis, is only defined clinically. In this study, we identified the differentially expressed genes(DEGs) in stages under benign or malignant ameloblastoma compared with normal patient using ordered differential display(ODD) reverse transcription(RT)-PCR and GeneFishing™ technology. ODD RT-PCR is rather effective when the investigation of samples containing very small amounts of total RNA must be accomplished. ODD RT-PCR used the means of amplification with anchored T-primer and adaptor specific primer, bearing definite two bases at their 3' ends and so this method could display differential 3' -expressed sequence taqs(ESTs) patterns without using full-length cDNAs. Compared with standard differential display, ODD RT-PCR is more simple and have enough sensitivity to search for molecular markers by comparing gene expression profiles. However, this method required much effort and skill to perform. GeneFishing™ modified from DD-PCR is an improved method for detecting differentially expressed genes in two or more related samples. This two step RT-PCR method uses a constant reverse primer(anchor ACP-T) to prime the RT reaction and arbitrary primer pairs(annealing control primers, ACPs) during PCR. Because of high annealing specificity of ACPs than ODD RT-PCR, the application of GeneFishing™ to DEG discovery generates reproducible, authentic, and long(100bp to 2kb) PCR products that are detectable on agarose gels. Consequently, various DEGs observed differential expression levels on agarose gels were isolated from normal, benign, and malignant tissues using these methods. The expression patterns of the some isolated DEGs through ODD RT-PCR and GeneFishing™ were confirmed by Northern blot analysis and RT-PCR. The results showed that these identified DEGs were implicated in ameloblastoma neoplasm processes. Therefore, the identified DEGs will be further studied in order to be applied in candidate selection for marker as an early diagnosis during ameloblastoma neoplasm processes.

정량적 게이트 심근관류 스펙트 검사에서 관류결손이 좌심근질량 측정과 부하 후 좌심실 용적 및 구혈률에 미치는 영향

안병철,배선근,이상우,정신영,이재태,이규보 대한핵의학회 2002 핵의학 분자영상 Vol.36 No.6

목적: 심질환의 예후 인자로 알려진 좌심근 질량 및 좌심실 구혈률을 구할 수 있는 게이트 심근관류 스펙트는 관류결손에 의해 영향을 받을 수 있다. 또한 관류결손을 일으키는 심혈관질환은 안정시와 부하후 좌심실 기능에 영향을 줄 수 있다. 본 연구는 심근관류 스펙트상 관류결손을 가진 환자를 대상으로 하여, 첫째 관류 결손의 크기와 가역성 여부에 따른 안정시와 부하 후 게이트 심근관류 스펙트로 구한 좌심근 질량의 차이를 알아 보았으며, 둘째, 관류결손의 가역성 여부에 따른 안정시와 부하 후 게이트 심근관류 스펙트로 얻은 좌심실 용적과 좌심실 구혈률의 상관성 및 차이를 비교해 보았다. 대상 및 방법: 심근관류 스펙트상 관류 결손이 있는 환자 46명을 대상으로 하였다. 대상 환자 가운데 남자가 34명(73.9%)이었고 평균연령은 64±9세였으며, 40명(87.0%)은 가역성 관류결손이었다. 게이트 심근관류 스펙트는 740MBq의 Tc-99m MIBI를 투여한 후 이중헤드 감마카메라(Vertex Plus, ADAC,USA)를 이용하여 영상자료를 얻었다. 좌심근 질량, 이완기말/수축기말 좌심실 용적 및 좌심실 구혈률은 AutoQUANT 프로그램을 이용하여 구하였다. 결과: 안정시와 부하 후 게이트 심근관류 스펙트로 구한 좌심근 질량은 뛰어난 상관성을 나타내었다. 가역적 관류결손 환자보다 고정 관류결손 환자가, 관류 결손의 크기가 20%미만인 환자가 관류 결손크기가 20% 이상은 환자보다 두 값 사이에 더 높은 상관성이 있었다. 고정 관류결손을 가진 환자는 안정시와 부하 후 게이트 심근관류 스펙트에서 얻은 좌심실 이완기말 용적, 좌심실 수축기말 용적, 좌심실 구혈률에 차이가 없었으나, 가영적 관류결손을 가진 환자에서는 안정시와 부하후 게이트 심근관류 스펙트에서 얻은 좌심실 구혈률에 유의한 차이가 있었다. 가역적 관류결손 환자 40명 가운데 10명(25%)에서는 부하후 게이트 심근관류 스펙트에서 구한 좌심실 구혈률이 안정시에 구한 좌심실 구혈률보다 5% 이상 낮았다. 고정관류 결손환자는 가역적 관류 결손환자에 비해 안정시와 부하 후 좌심실 용적과 구혈률에 더 높은 상관성을 나타내었다. 결론: 관류결손은 게이트 심근관류 스펙트를 이용한 좌심근 질량측정에 영향을 미칠 수 있으며, 고정 관류 결손을 가진 환자 보다 가역적 관류 결손을 가진 환자에서 심근부하 후 과심실 구혈률 감소가 더 빈번하게 발생됨을 알 수 있었다. Purpose: The presence of perfusion defect ma influence the left ventricular mass (LVM) measurement by quantitative gated myocardial perfusion SPECT (QGS), and ischemic myocardium, usually showing perfusion defect may produce post-stress LV dysfunction. This study was aimed to evaluated the effects of extent and reversibility of perfusion defect on the automatic measurement of LVM by QGS and to investigate the effect of reversibility of perfusion defect on post-stress LV dysfunction. Subjects and Methods: Forty-six patients (male/female=34:12, mean age=64years) with perfusion defect on myocardial perfusion SPECT underwent rest and post-stress QGS. Forty patients (87%) showed reversible defect. End-diastolic volume (EDV), end-systolic volume (ESV), LV ejection fraction (EF), and LV myocardial volume were obtained from QGS by AutoQUANT program, and LVM was calculated by multiplying the LV myocardial volume by the specific gravity of myocardium. Results: LVMs measured at rest and post-stress QGS showed good correlation, and higher correlation was founded in the subjects with fixed perfusion defect and with small defect (smaller than 20%). There were no significant differences in EDVs, ESVs and EFs between obtained by rest and post-stress QGS in patients with fixed myocardial defect. Whereas, EF obtained post-stress QGS was lower than that by rest QGS in patients with reversible defect and 10 (25%) of them showed decreases in EF more than 5% in post-stress QGS, as compared to that of rest QGS. Excellent correlations of EDVs, ESVs, EFs between rest and post-stress QGS were noted. Patients with fixed defect had higher correlation between EDVs, ESVs, EFs than patients with reversible defect. Conclusion: These results suggest that perfusion defect can affect LVM measurement by QGS and patients with reversible defect shows post-stress LV dysfunction more frequently than patients with fixed perfusion defect. (Korean J Nucl Med 2002;36;381-91)

시판 항생제들의 대추나무빗자루병 치료약제 가능성 비교

박철하,이세표,차병진 충북대학교 농업과학기술연구소 1994 農業科學硏究 Vol.11 No.2

To minimize the trial and error by farmers who have no other choice but to use antibiotics for human-disease control, because no agricultural tetracycline for jujube witches-broom disease is available at this point. therapeutical efficacy of several commercial antibiotics against the disease through trunk injection was evaluated. The efficacy of human tetracyclines in trunk injection of jujube tree for witches-broom therapy varied to some extnet. Tetracyclines for intravenous or subepidermal injection showed the strongest symptom-suppression effect. Those, however, were not practical because of high price. On the other hand, tetracyclines in capsule were much cheaper than the former and can be used practically. For trunk injection, just the powder in capsule should be dissolved in clean water and then filtered through cheese cloth or tissue papaer to remove the sediments unnecessary for trees. Otherwise, the sediment would clog the injection hole and the feeder tube. In this experiment. tetracycline HCl of Glaxo made the least sediment in the solution. In addition, the plastic bottle to contain Korean wine was much cheaper and more practical than the commercial one for tree injection.

우치의 근관에서 도말층의 존재가 수산화칼슘의 상아질 투과에 미치는 영향

권병구,윤태철,박동수 大韓齒科保存學會 1997 Restorative Dentistry & Endodontics Vol.22 No.1

스포츠센터 직원의 임금만족 선행요인이 임금만족과 조직유효성에 미치는 영향

정병철,김장환,박진기 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.4

This study aims to find out the individual and structural variables in the determination of the wages of the sports center staffs, to analyze the precedent factors of the wage satisfaction(variables of job inputs, the variables of job characteristics, the comparisons with the objects compared, the variables of the organization characteristics), and to examine the effects of the wage satisfaction(welfare programs, the levels of wages, the increase of wages, the management of wages) on the indexes of the organization efficiency such as the will to leave jobs, the outcomes of jobs, the organizational committment and so on. The materials were analyzed by using the programs of SPSS/PC+11.0 and LISREL 8.30 by means of the covariance structure analysis. The results of the inspection of the hypotheses through these methods of the study and the analyses of the materials are as follows: First, the results of the comparative analyses of the satisfaction in respect to the individual and structural variables show that females, the older groups, and the married groups have higher satisfaction than males, the younger groups and the unmarried groups. And the higher positions produce higher wage satisfaction. The higher schooling and the longer careers produce lower wage satisfaction, whereas, in terms of the salary management, they show higher satisfaction. In terms of the experiences of leaving jobs, the groups with no such experiences show high wage satisfaction, and the groups with experiences of leaving jobs two times show the lowest satisfaction. In terms of the methods to join the companies, the groups scouted show the highest wage satisfaction and the groups employed by public examinations show the lowest wage satisfaction. Second, the analyses of the precedent factors affecting the wage satisfaction show that the higher awareness of the job inputs, the job characteristics, and the comparison factors with the groups compared show the less satisfaction with the levels of wages. And the higher awareness of the factors of the organizational characteristics show the higher satisfaction. The analyses of the effects of the wage satisfaction on the organizational efficiency show that the higher wage satisfaction has a negative relation with the will to leave jobs, and a positive relation with the outcomes of jobs and the organizational committment.