Effect of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System

Byeong-chunLee,Kwang-teakLim,Sung-keunKang,Woo-sukHwang 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.1

In this study,we examined the effects of a two-stepculture system, which involves the use of differentculture media for early cleavage and later stageembryos, on the in vitro development of bovineembryos. We also investigated the effect of glucose,phosphate and citrate on the in vitro early develop-mental period of bovine embryos in a two-stepculture system. Moreover, the supplementation ofdifferent protein sources (BSA-V, BSA-FAF and FBS)during IVC did not affect the frequency of blastocystdevelopment. Using two-step culture, embryos werecultured in protein-free media for an initial 5 days.This was then followed by the same culture media oran FBS supplemented media. The developmentalrates of blastocysts in the FBS containing group weresignificantly higher than in the replaced with noserum containing group. Embryos cultured in mSOFsupplemented with 1.5 mM glucose plus 1.2 mMphosphate were significantly inhibited.The inhibitionof developmental competence by glucose plus phos-phate was consistent with the existence of 0.5 mMsodium citrate. This study indicates that a two-stepculture system,which applies different conditions forearly cleavage embryos, i.e., serum-free media, vs.later stage embryos, with serum containing media,may be effective for in vitro production systems. Inaddition, the developmental competence of bovineembryos was depressed in the presence of glucoseplus phosphate as compared to either alone or theabsence of both. Therefore, the avoidance of thisnegative effect should allow more optimal conditionsto be developed for in vitro production.*Corresponding author: Byeong-chun Lee

Characterization of the European type of maternal lineage evident in extant Jeju native pigs

Byeong‐Woo Kim,In‐Cheol Cho,Moon‐Sung Park,Tao Zhong,임현태,Sung‐Soo Lee,Hee‐Bok Park,Moon‐Suck Ko,Jun‐Heon Lee,Jin‐Tae Jeon 한국유전학회 2011 Genes & Genomics Vol.33 No.2

Using a partial D‐loop sequence of mtDNA, an intensive analysis was conducted of the maternal lineages of Jeju native pigs (JNPs) from Korea. In total, 100 mtDNA sequences were obtained from Asian wild boars (AWBs), European wild boars (EWBs), Asian domestic pigs (ADPs), European domestic pigs (EDPs), and JNPs and were used for phylogeny and network analyses. Two distinct JNP groups - one (JNPA) in the Asian cluster and the other (JNPE) in the European cluster - were identified in the estimated phylogenetic tree and network. The maternal lineage of JNPE was the closest to that of EWB and a clear haplogroup was identified that shared an identical haplotype (hap04) among 15 individuals of JNPE and 2 individuals of EWB. A Landrace and an EWB shared hap03with a JNPE. EWB, Landrace, Large White, and Duroc formed two clear haplogroups with JNPE in a parsimonious medianjoining network analysis, suggesting that an obvious maternal contribution of EDP has occurred in JNPE in recent years. A pair‐wise mismatch analysis also indicated that JNPE may have experienced a sudden population expansion, suggesting a more recent establishment compared with the gradual population expansion of JNPA. The JNPE group therefore should be further evaluated in order to decide whether this group should be culled or accepted into further programs for maintenance of the JNP population as a pure breed.

효모시스템에서 Human Transglutaminase C(TGase 2)의 발현에 관한 연구 : C-말단부위의 결손효과 An Effect of a Domain from Carboxyl Terminal Deletion of the Enzyme

우상규,정선미,이상기,안병윤,김희철 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

Transglutaminase(TGase Ⅱ)는 펩타이드-결합 글루타민 잔기와 펩타이드-결합 라이신잔기 및 1차 아민류를 포함하는 다양한 화합물과의 교차결합에 의하여 단백질의 변형을 촉매하는 효소이다. 동물세포에는 구별가능한 5종류의 TGase가 존재한다[TGase Ⅰ(또는 K), Ⅱ(C), Ⅲ(E), Ⅳ(P)와 혈장단백 ⅩⅢa]. 그들은 아미노산 서열에서 N-말단에 약 70%의 상동성을 갖고 있으며, C-말단의 경우에는 거의 상동성이 부위(N-말단 2/3)와 비상동성 부위(C-말단 1/3 부위)의 역할을 알아보기 위해 전체효소 또는 C-말단이 결손된 형태의 효소를 Saccharmoyces cerevisiae에서 발현 하고자 하였다. Southern blot, 역전사 중합 연쇄 반응, Western blot등을 통해 전체효소 및 C-말단 결손형 효소가 형질전환된 효모에서 발현되는 것을 확인하였다. Succinylated casein에 [1,4-^14C]-putrescine이 통합되는 반응을 이용한 활성 측정에서 C-말단이 결손된 TGase Ⅱ는 같은 세포 농도에서 전체 TGase Ⅱ 효소와 비교하여 유사한 수준의 활성을 나타내었다. 이러한 결과들은 사람 TGase Ⅱ의 경우 N-말단의 높은 상동성부위가 효소의 역가결정에 중요하다는 것을 나타내 준다. 효모시스템에서 발현한 결과 효소의 역가가 나타났으나 높지 않았으며 발현된 양이 극히 낮아서 단백질의 정제나 특성연구를 위하여 발현벡터의 개량을 포함한 방법의 개선이 필요하다고 사료된다. 흥미롭게도 TGase Ⅱ의 발현이 효모의 성장을 저해하는 것으로 나타나 이러한 현상이 표피세포의 각질화에 수반된 세포의 죽음과 유관한지의 가능성에 대한 보다 자세한 연구가 필요하다. In an effort to understand the role of the conserved domain and of the heterologous one-third part of the carboxy terminal domain of transglutaminase C (TGase Ⅱ), attempts were made to express TGase Ⅱ cDNA of human origin in yeast Saccharmoyces cerevisiae as in a full-length form as well as in a form of C-terminal truncation. The 2μ-based expression plasmids which contained the TGase Ⅱ cDNA under the gal inducible promoter were introduced into yeast and the maintenance of the full-length and truncated form of the TGase Ⅱ gene plasmids were confirmed by Southern blot. The expression of e results indicate that the N-terminal homologous domain of human TGase Ⅱ retains a catalvtically active domain. The level of TGase Ⅱ expressed in yeast, however, was far lower than satisfactory and other expression system should be sought further characterization of the enzyme. The negative effect of TGase Ⅱ on the growth of yeast is interesting with respect to the physiological effect of TGase Ⅱ in the cornification f epidermal keratinocytes.

정량적 게이트 심근관류 스펙트 검사에서 관류결손이 좌심근질량 측정과 부하 후 좌심실 용적 및 구혈률에 미치는 영향

안병철,배선근,이상우,정신영,이재태,이규보 대한핵의학회 2002 핵의학 분자영상 Vol.36 No.6

목적: 심질환의 예후 인자로 알려진 좌심근 질량 및 좌심실 구혈률을 구할 수 있는 게이트 심근관류 스펙트는 관류결손에 의해 영향을 받을 수 있다. 또한 관류결손을 일으키는 심혈관질환은 안정시와 부하후 좌심실 기능에 영향을 줄 수 있다. 본 연구는 심근관류 스펙트상 관류결손을 가진 환자를 대상으로 하여, 첫째 관류 결손의 크기와 가역성 여부에 따른 안정시와 부하 후 게이트 심근관류 스펙트로 구한 좌심근 질량의 차이를 알아 보았으며, 둘째, 관류결손의 가역성 여부에 따른 안정시와 부하 후 게이트 심근관류 스펙트로 얻은 좌심실 용적과 좌심실 구혈률의 상관성 및 차이를 비교해 보았다. 대상 및 방법: 심근관류 스펙트상 관류 결손이 있는 환자 46명을 대상으로 하였다. 대상 환자 가운데 남자가 34명(73.9%)이었고 평균연령은 64±9세였으며, 40명(87.0%)은 가역성 관류결손이었다. 게이트 심근관류 스펙트는 740MBq의 Tc-99m MIBI를 투여한 후 이중헤드 감마카메라(Vertex Plus, ADAC,USA)를 이용하여 영상자료를 얻었다. 좌심근 질량, 이완기말/수축기말 좌심실 용적 및 좌심실 구혈률은 AutoQUANT 프로그램을 이용하여 구하였다. 결과: 안정시와 부하 후 게이트 심근관류 스펙트로 구한 좌심근 질량은 뛰어난 상관성을 나타내었다. 가역적 관류결손 환자보다 고정 관류결손 환자가, 관류 결손의 크기가 20%미만인 환자가 관류 결손크기가 20% 이상은 환자보다 두 값 사이에 더 높은 상관성이 있었다. 고정 관류결손을 가진 환자는 안정시와 부하 후 게이트 심근관류 스펙트에서 얻은 좌심실 이완기말 용적, 좌심실 수축기말 용적, 좌심실 구혈률에 차이가 없었으나, 가영적 관류결손을 가진 환자에서는 안정시와 부하후 게이트 심근관류 스펙트에서 얻은 좌심실 구혈률에 유의한 차이가 있었다. 가역적 관류결손 환자 40명 가운데 10명(25%)에서는 부하후 게이트 심근관류 스펙트에서 구한 좌심실 구혈률이 안정시에 구한 좌심실 구혈률보다 5% 이상 낮았다. 고정관류 결손환자는 가역적 관류 결손환자에 비해 안정시와 부하 후 좌심실 용적과 구혈률에 더 높은 상관성을 나타내었다. 결론: 관류결손은 게이트 심근관류 스펙트를 이용한 좌심근 질량측정에 영향을 미칠 수 있으며, 고정 관류 결손을 가진 환자 보다 가역적 관류 결손을 가진 환자에서 심근부하 후 과심실 구혈률 감소가 더 빈번하게 발생됨을 알 수 있었다. Purpose: The presence of perfusion defect ma influence the left ventricular mass (LVM) measurement by quantitative gated myocardial perfusion SPECT (QGS), and ischemic myocardium, usually showing perfusion defect may produce post-stress LV dysfunction. This study was aimed to evaluated the effects of extent and reversibility of perfusion defect on the automatic measurement of LVM by QGS and to investigate the effect of reversibility of perfusion defect on post-stress LV dysfunction. Subjects and Methods: Forty-six patients (male/female=34:12, mean age=64years) with perfusion defect on myocardial perfusion SPECT underwent rest and post-stress QGS. Forty patients (87%) showed reversible defect. End-diastolic volume (EDV), end-systolic volume (ESV), LV ejection fraction (EF), and LV myocardial volume were obtained from QGS by AutoQUANT program, and LVM was calculated by multiplying the LV myocardial volume by the specific gravity of myocardium. Results: LVMs measured at rest and post-stress QGS showed good correlation, and higher correlation was founded in the subjects with fixed perfusion defect and with small defect (smaller than 20%). There were no significant differences in EDVs, ESVs and EFs between obtained by rest and post-stress QGS in patients with fixed myocardial defect. Whereas, EF obtained post-stress QGS was lower than that by rest QGS in patients with reversible defect and 10 (25%) of them showed decreases in EF more than 5% in post-stress QGS, as compared to that of rest QGS. Excellent correlations of EDVs, ESVs, EFs between rest and post-stress QGS were noted. Patients with fixed defect had higher correlation between EDVs, ESVs, EFs than patients with reversible defect. Conclusion: These results suggest that perfusion defect can affect LVM measurement by QGS and patients with reversible defect shows post-stress LV dysfunction more frequently than patients with fixed perfusion defect. (Korean J Nucl Med 2002;36;381-91)

고주파수 PWM 제어를 이용한 ABS의 특성 평가에 관한 연구

김병우,이용주,박호 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.2

In general the surge pressure generated in hydraulic systems causes noise, vibration and odd effect to the system. To reduce the surge pressure, high frequency PWM control of 20KHz was attempted. To estimate the braking noise caused by surge, a vehicle equipped with on-board ABS hydraulic modulator has been experimented with respect to the various breaking condition. Though this experiments, it was found that breaking noise has been reduced using high frequency PWM control method compare with low frequency method. To evaluate high frequency control in practice, including verification of general functionality, EM tests was experimented. Its was found that it is necessary to have the solution to electromagnetic interference(EMI) generated by switching elements.

鋪裝道路(Asphalt) 沿邊의 公害 物質이 蠶作에 미치는 影響 : (Ⅱ) 公害 物質이 繭層比率에 미치는 影響 (Ⅱ) The effect of air pollution on CoCoon layer ratio

文炳圓,金鎭雨,崔周鎬 진주산업대학교 1983 論文集 Vol.21 No.-

1) 鋪裝 沿邊의 公害物質이 견층비율에 큰 영향을 나타난다. 2) 春蠶, 秋蠶 晩秋蠶 飼育에 대한 公害의 影響은 매우 차가 심하여 견층 비율은 각 계절간에 다양하게 나타났다. 3) 桑園 肥培管理를 철저히하여 누에 生理에 유의하여 公害를 다소나마 防止해야겠다. 4) 綜合的인 公害 방지책을 硏究해야겠다. This study was Carried out to investigate the effect of airpollution substance on raising silkworms and the way reducing the damoge of a public nuisance. The result obtained were summarized as follows: 1. Imjury of air pollution substanel at the areaa asphalt road was increased more than Cocoon layer ratio of a hilly country. 2. As the effect of public hagard about the raising silkworms in the spring, fall and late­fall showed very difference, Cocoon lager ratio was varietal to the treatment of season all. 3. I think that we must reduce the damage of air pallution as think over the silkworm physiology.

한국신용협동조합의 현황과 발전방향에 관한 연구

홍금우,정병국,구달모 조선대학교 경영경제연구소 2002 經營經濟硏究 = Management and economics research Vol.25 No.1

볼 엔드밀 가공시 채터가 가공면에 미치는 영향

박춘우,홍남표,김병희 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.A

This paper deals with the study on the culling characteristics in ball-end milling process. First of all, the effects of the geometric cutting conditions such as the spindle speed, feedrates on the surface integrity and machining stability were evaluated by the analytical and the experimental approaches. A large amount of experimental sets are performed to evaluate the effects of chatter phenomenon on the machined surface. The optical microscope and the surface roughness measuring machine are used to measure the surface integrity and roughness of the machined surfaces

STS 316의 크리프 파단 거동에 미치는 장시간 시효의 영향

임지우,임병수 成均館大學校 科學技術硏究所 1999 論文集 Vol.50 No.1

Although type 316 stainless steel is widely used in reactors of petrochemical plants and pipes of steam power plants, the effect of precipitates which form during the long term exposure at service temperature, on creep properties is not known sufficiently. In this study, to investigate the influence of prior aging on the microstructure and creep properties, the 316 stainless steel was first solutionized at 1130℃ for 20 minutes and then the aged specimens for different time of 0hr, 100hrs, 1000hrs and 2200hrs were prepared at 750℃. After heat treatments, the tensile tests both at room temperature and 650℃, the constant load creep rupture tests were carried out. From the experimental results, following conclusions were obtained. (1) The rupture life of 100 hour aged specimens was found to be the shortest. It is resulted from the highest minimum creep rate. And the 0hr aged specimens showed the longest rupture life, followed by 1000hr aged and 2200hr aged specimens. (2) The value of the creep stress exponent, n, decreased as the aging time increased. The n values for the 0hr, 100hr, 1000hr, 2200hr aged specimens were to be measured 13.5, 5.7, 4.4 and 2.9. (3) The creep activation energy varied with the aging time. The activation energies for 0hr, 100hr, 1000hr and 2200hr aged specimens were calculated to be 679.1, 421.6, 527.5 and 517.2 kJ/mol.

조직구 증식증 : Hand-Schuller-Christian 병에 대한 증례보고

백병주,김재곤,정미우 大韓小兒齒科學會 1988 大韓小兒齒科學會誌 Vol.15 No.1

Histiocytosis X is a proliferative disorder of histiocytes that consisting of three clinical syndromes which shared several basic clinical and histopathologic features ; Letterer-Siwe disease, Hand-Schuller-Christian disease, and eosinophilic granuloma Hand-Schuller-Christian disease commonly affects older children between the ages of 5 and 10. but may be seen in any age group. It typically follows a more chronic courses. 7 years old female are refered to the Dept. of Pedodontics. College of Dentistry. Chonbuk National University due to the severe tooth mobility on whole dentition. clinical examination., radiographic and histopathologic findings were used to evaluation of the lesion. The purpose of this article is to report a case of histiocyte involving oral cavity and to review the procedure involved in the diagnosis and treatment of this disorder.