직접치수복조재에 따른 비글견 치수의 조직반응에 대한 연구

배지현,김영균,윤필영,조병훈,최용훈 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.1

본 연구에서는 인위적으로 노출시킨 비글견의 치수조직을 기존의 수산화칼슘제재와 MTA, 접착성 레진, 광중합형 수산화칼슘제재를 사용하여 직접치수복조한 후 각 재료에 따른 치수의 반응을 광학현미경 하에서 조직학적으로 관찰하여 비교분석하였다. 2마리 비글견의 36개 치아를 이용하여, 실험적으로 치수를 노출시키고 노출된 치수에 치수복조재를 적용한 후 와동은 복합레진으로 충전하여 직접치수복조술을 시행하였다: (1) Mineral Trioxide Aggregate (MTA: ProRoot MTA, Dentsply, Tulsa, USA), (2) Clearfil SE Bond (Dentin adhesive system: Kuraray, Osaka, Japan), (3) Ultra-Blend (Photopolymerized Calcium hydroxide: Ultradent, South Jordan, USA), (4) Dycal (Quick setting Calcium hydroxide: LD Caulk Co., Milford, USA). 희생전 90일, 30일, 7일 전에 각 복조재별로 3개씩의 와동을 충전하였고 비글견을 희생시키고 조직시편을 제작하였다. 시편을 H&E 염색 후 광학 현미경으로 치수염증반응과 경조직 형성 정도를 관찰하였다. MTA 군은 초기에는 경조직 형성이 관찰되지 않고 치수조직의 위축과 부분괴사가 관찰되었으나 시간이 지나면서 경조직 형성이 관찰되었다. Clearfil SE Bond군은 초기에 염증세포의 침윤과 치수세포의 괴사를 관찰할 수 있었고 시간이 지날수록 치수 세포의 괴사가 더욱 진행한 양상을 보였다. Ultra-blend 군과 Dycal 군은 MTA 군과 비슷하게 초기에는 경조직 형성을 보이지 않고 중등도의 염증반응이 관찰되었으며 시간이 지나면서 경조직 형성이 관찰되었다. MTA, 수산화칼슘제재와 광중합 수산화칼슘제재는 초기의 치수염증반응 이후 조상아세포층, 상아질교 형성을 보여 직접치수복조재로 적당하였으며, 접착성 레진은 심한 염증반응과 치수 조직의 괴사 양상을 보여 직접치수복조재로 부적당하다는 결론을 얻을 수 있었다. The purpose of this study was to evaluate the pulp tissue reaction to direct pulp capping of mechanically exposed beagle dogs' pulp with several capping materials. A total of 36 teeth of 2 healthy beagle dongs were used. The mechanically exposed pulps were capped with one of the followings: (1) Mineral Trioxide Aggregate (MTA: ProRoot MTA. Dentsply, Tulsa, USA), (2) Clearfil SE Bond (Dentin adhesive system: Kuraray, Osaka, Japan), (3) Ultra-Blend (Photo-polymerized Calcium hydroxide: Ultradent, South Jordan, USA), (4) Dycal (Quick setting Calcium hydroxide: LD Caulk Co., Milford, USA) at 7, 30, and 90 days before sacrificing. The cavities were restored with Z350 flowable composite resin (3M ESPE, St. Paul. MN, USA). After the beagle dogs were sacrificed, the extracted teeth were fixed, decalcified, prepared for histological examination and stained with HE stain. The pulpal tissue responses to direct pulp capping materials were assessed. In MTA calcium hydroxide, and photo-polymerized calcium hydroxide groups, initial mild inflammatory cell infiltration, newly formed odontoblast-like cell layer and hard tissue bridge formation were observed. Compared with dentin adhesive system, these materials were biocompatible and good for pulp tissue regeneration. In dentin adhesive system group, severe inflammatory cell infiltration, pulp tissue degeneration and pulp tissue necrosis were observed. It seemed evident that application of dentin adhesive system in direct pulp capping of beagle dog teeth cannot lead to acceptable repair of the pulp tissue with dentine bridge formation.

키 프레임을 이용한 동영상 검색에 관한 연구

김희수,김형균,전병균,이호현 광주보건대학 2004 論文集 Vol.29 No.-

The primary goal of video parsing is to accurately detect fast/gradual scene changes to index the video data so that future retrieval may be performed efficiently. Due to the sheer volume of the video data, however, the goal of accurate detection must be met by some real-time (fast) automatic (or at least semi-automatic) video parsing mechanism. For this purpose, I have developed a fast algorithm to detect gradual scene change and also detect camera motions to reduce false positives (falsely declaring a scene-change). In this dissertation, we propose a new approach that detects the change of scene which contains fast motion, zooming, dissolve in AVI video sequence. The proposed scheme adopts the feature matching technique which ALH(Average of Luminance Histogram) in each frame with those in the previous frame. Experimental results show that the proposed method performs better in detection scene changes than other conventional methods.

P2P 기반 Mobile System 구현

황인선,이호현,전병균,김형균 광주보건대학 2004 論文集 Vol.29 No.-

P2P network environments provide mobile users with direct data transmission and sharingfacilities and those environments can be classified into hybrid P2P network environments and pure P2P network environments according to the arbitration mechanism among the peers in the network. In pure P2P network environments, however, each peer directly connects to another peer and gets services without server intervention, and so, network isolation can occur when the mediating peer fails to work. In this paper, we propose a scheme for each peer to keep connection to other peers continuously by maintaining IP addresses of its neighbor peers and connecting to the peers when the mediating peer fails to work. Although the P2P application that uses our proposed framework should obtain one or more IP addresses of the neighbor peers manually, after instantiation, the application can do its job while maintaining connection to the network continuously and automatically. In order to improve these weaknesses, we introduce LBS(Location Based Service) and mobile P2P(Peer to Peer)service into the existing systems, then design and implement the enhanced system for mobile SFA using them. Through this improvement, our system makes it possible for customers or sales agents to quickly share various information and increases the efficiency of sales activity by facilitating the competition and cooperation among sales agents and reducing time-cost.

소성시간에 따른 재생시멘트의 물성 평가

임승준,김세훈,오상균,김정길,강병희 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

Serious problems of the environment protection and resource exhaustion are exhibited, due to the increase of the construction materials and activation of the remodeling, recently. Especially, most of the advanced countries, recycling plan for the waste concrete is vigorously progressing. The recycle of domestic waste concrete is, however, still in an early stage, and it has been only partially being used for the road fillers. As a counter-plan of activating recycled concrete, we have confirmed the hydration possibility of the waste concrete powder from the experiment on recycling the aggregate powder since 2000. Also, we have known that the strength is increasing when the heating time is longer, and baking temperature maintain in 700℃. The purpose of this study is to determine the most suitable production method controlling the duration of baking time for recycled cement from the compressive strength, X -ray diffraction and ingredient analysis test.

세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

한국에서 동물간호사제도의 필요성과 자격시험에 대한 분석 및 평가

이성호,박영석,박병권,곽명순,강종일,조성진,김선균,김병수 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

The study examined qualification test of the 1st term Animal Nurse Technician and conducted questionnaire survey as well as site investigation to settle Animal Nurse Technician system in Korea. The objects were undergraduates, graduates and director of animal hospital. 115 applicants applied to the qualification test of animal specialists, and 50 applicants passed the test to record 43.47% of ratio of successful applicants. The subjects having relative difficulty were anatomy physiology and public hygiene to record 19 points in average: In particular, the anatomy physiology recorded 35 points of the highest score and 6 points of the lowest score to have the highest deviation and to require adjustment of difficulties. 89.2% of the interviewee supported needs of animal nurse technician system in Korea to show positive response. At the survey of actual conditions of recruitment of animal nurse technicians at animal hospitals, 28.6% of the interviewee was employing the technicians, and 35.7% had employment plan, and 32.1% wanted to employ them under good conditions. In other words, 96 4% of the interviewee responded positively, while no more than 3.6% did negatively. In Korea, the animal nurse technicians had roles as follows: The animal hospitals gave priority to reception service and customer controls followed by medical examination support,whilethecollegesdidtomedicalexaminationsupport. To differentiate hospitals in accordance with the increase of number of animal hospitals, the animal hospitals should employ animal nurse technicians having specialty and qualifications on animal nurse technician examination. In particular, the roles and demand of animal nurse technicians were expected to grow because of developed technologies of veterinary medical science, development of medical supplies and instrument, and the increase of aged animals, etc. Therefore, job analysis on animal nurse technicians should be done to adjust the subjects of qualification test and to develop questions.

Characterization of X-linked RNA Transcripts in Matured Bovine Spermatozoa

Byeong-Gyun Jeon,Mohana Kumar,Gyu-Jin Rho 한국동물번식학회 2011 Reproductive & developmental biology Vol.35 No.3

Comparisons of Development Potential in Bovine SCNT Embryos using Donor Cells treated with Different Demethylating Inhibitors

Byeong-Gyun Jeon,Gie-Joon Jeong,Gyu-Jin Rho 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.3

To improve the developmental potential of bovine somatic cell nuclear transfer (SCNT) embryos, this study compared the developmental rates to blastocyst stage in the SCNT embryos using donor fibroblasts treated with 5-azacytidine (5AC) and S-adenosylhomocysteine (SAH) at different concentrations. Their reprogramming efficiency level was investigated with level of telomerase activity. Donor fibroblasts isolated from adult ear skin of a cow were exposed to 5AC and SAH at different concentrations during 2 passages. After nuclear transfer into enucleated recipient oocytes, the cleavage and developmental rates were significantly (p<0.05) decreased in the SCNT embryos using 5AC-treated fibroblasts (5AC-SCNT embryos), compared with those of non-treated control (control-SCNT embryos) and SAH-treated fibroblasts (SAH-SCNT embryos). The developmental rates to blastocyst stage tended to be slightly increased in the SAH-SCNT embryos at each of the concentrations, and especially, the developmental rates in the SCNT embryos using 1.0 mM SAH-treated fibroblasts were significantly (p<0.05) higher than that of control SCNT embryos. The mean numbers of total and ICM cell in blastocysts were also significantly (p<0.05) decreased in the 5AC-SCNT embryos, compared with those of other SCNT blastocysts. Further, the level of telomerase activity was also significantly (p< 0.05) decreased in the 5AC-SCNT embryos than those of control and SAH-SCNT embryos. Whereas, a significantly (p<0.05) up-regulated telomerase activity was observed in SAH-SCNT embryos, compare with that of control-SCNT embryos. In conclusion, SCNT embryos using hypomethylated donor cells with SAH, not 5AC, may improve the developmental potential and reprogramming efficiency.

Variation of Transcribed X-linked Genes in Bovine Embryos Cloned with Fibroblasts at Different Age and Cell Cycle

Byeong-Gyun Jeon,Gyu-Jin Rho 한국동물번식학회 2011 Reproductive & developmental biology Vol.35 No.2

Nuclear Modeling and Developmental Potential of Bovine Somatic Nuclear Transfer Embryos Cloned by Two Different Activation Methods

Byeong-Gyun Jeon,Gyu-Jin Rho 한국동물번식학회 2011 Reproductive & developmental biology Vol.35 No.1