Comparison of Streptococcus mutans Biofilm Formation on Dental Materials

Byeng-Ken Cho,Myung-Sook Kim,Ja-Won Cho 대한예방치과학회 2015 International Journal of Clinical Preventive Denti Vol.11 No.4

Objective: The authors had conducted this study for the purpose to compare the adhesion level of Streptococcus mutans to various oral appliance materials. Methods: Six kinds of materials were used in this study. Bioplast, Sofliner Tough Medium, Duran, Ortho-Jet, Lucitone 199, Tokuyama Rebase II were selected. S. mutans ATCC 25175 was purchased from American Type Culture Collection and cultured with brain-heart infusion (BHI) broth. For biofilm formation, S. mutans was cultured in BHI broth supplemented 1% sucrose. S. mutans was mixed BHI broth including 2% sucrose after cultivation for overnight. And, formation and observation of whole bacteria and S. mutans in salivary biofilm on specimens, and measurement of saliva protein on specimen were carried out. Results: The adhesion level of S. mutans and whole bacteria is lower in resilient materials than in rigid materials. Sportguard wafer is the lowest adhesion level of S. mutans and whole bacteria among the resilient materials. There were no differences of adhesion level of S. mutans and whole bacteria among the rigid materials (p<0.05). Saliva protein was more attached on rigid materials than on resilient materials. Conclusion: It was recommended to clean the oral appliance periodically.

개인 트레이를 이용한 불소도포 효과에 관한 실험적 연구

조병근 ( Byeng Ken Cho ),서은주 ( Eun Ju Seo ) 한국치위생학회 2001 한국치위생학회지 Vol.1 No.2

Changes in Oral Malodor According to Menstrual Cycle for Women

Jin-Hee Oh,Byeng-Ken Cho,Woo-Ryung Park,Ja-Won Cho 대한예방치과학회 2014 International Journal of Clinical Preventive Denti Vol.10 No.2

Objective: The objective of this study is to investigate intraoral factors which take a severe part, general illness and smoking to exam the change of halitosis within the woman's menstural cycle. Methods: We examined the halitosis changes per menstruation cycle using the sensory evaluation and mechanical halitosis measurement. Results: According to the sensory evaluation, there were no significant changes per phase; menstruation, follicular, ovulation and luteal (p>0.05). According to the BB Checker (Tokuyama Soda Co. Ltd., Tokyo, Japan), there were no significant changes in the BB values per phase when it comes to the intra-oral gas, exhaled gas and nasal gas (p>0.05). According to the Oralchroma measurement, the mean of hydrogen sulfide was 1.46±2.25 ng/10 ml at the menstruation phase, 1.57±1.62 ng/10 ml at the follicular phase, 0.58±0.70 ng/10 ml at the ovulation phase and 1.25±2.57 ng/10 ml at the luteal phase, getting slightly lower, yet no statistical significance was recognized (p>0.05). According to the Oralchroma measurement, there was no significant change per phase in terms of the measured methyl mercaptan and dimethyl sulfide values (p>0.05). When the halitosis changes according to the menstruation cycle were checked, there were no significant periodic changes, yet H2S, the volatile sulfur compound, dropped a little during the ovulation phase. Conclusion: It is believed that the menstruation cycle may impact the halitosis changes as variables like systematic diseases and intra-oral factors are excluded; However, it may not have a great impact on the severity of halitosis.

The Recognition for the Dental Profession on the Students in Korea, Japan, Laos and Mongolia

Jung-Hee Seo,Byeng-ken Cho,Yeon-Soo Chang,Su-Kyung Jwa 대한예방치과학회 2013 International Journal of Clinical Preventive Denti Vol.9 No.3

Objective: In order to compare the recognition of the dental students studying dental medicine on dental profession depending on the politics, economy and culture of a country, the author selected dental students from 4 Asian countries. Methods: Including 60 from Korea, 59 from Japan, 60 from Laos and 60 from Mongolia, and conducted a survey using questionnaires to ask their current school life, their occupational view after graduation, and the image of dentist. Based on the analysis of 239 responses, the following conclusion was made. Results: It was found that more than half of Japanese respondents had someone in their families or relatives involving in the dental field. As for the motivation to enter dental college, it was indicated that Korean students were based on their academic grades in high schools, Japanese students on their aptitude, and the students of Laos and Mongolia on their aspiration for dental medicine. It was shown that 51.7% of Korean students, 90% of Laotian students and 81.7% of Mongolian students had high pride as dental students, while 59.3% of Japanese students answered that their pride as dental students were medium (p<0.05). Conclusion: It was found that there was some difference in the basic recognition of dental students, depending on the politics, economy and culture of a country. Although there is some limitation in generalizing the findings of this study, it is considered that the study results can be helpful as the basic data for the future exchange or opening of dental medicine, as all dental students considered international cooperation to be positive for the development of the dental community.

Amounts of Oral Microorganism according to Use of the Interdental Brush with Cethyl Pyridium Chloride Solution

Ji-Sook Jeon,Byeng-Ken Cho,Yun-Hwa Choi,Ji-Hyeon Park 대한예방치과학회 2015 International Journal of Clinical Preventive Denti Vol.11 No.2

Objective: Interdental brush can be used at proximal area of the interdental space and prosthodontic or orthodontic appliance where is hard to access by use of normal toothbrush. It is well known that lots of oral microorganism would be inside of gingival pocket at the proximal area and it should be eliminated with clean way. Methods: The author has measured the amounts of the oral microorganism at proximal area by use of adenosine triphosphate unit (SURE PlusTM) for 50 adults volunteers, after using the interdental brush and after sterilization with cethyl pyridium chloride (CPC) solution, through inserting the tip of the interdental brush into the CPC solution for a minute with shaking it. Amounts of oral microorganism at proximal area were measured as relative light units (RLU) unit and gingival index with Löe and Silness index and gingival pocket depth were measured, in order to find the relation between the amounts of oral microorganism and their Value, by use of Pearson’s co-relation coefficient. Results: The results were as followings: 1. Oral microorganism at the tip of interdental brush afterusing at the proximal area was checked as average 9,492.9±407.2 RLU and a little decreased value as 8,959.8±485.4 RLU on proximal area after applying of CPC solution (p<0.01). Otherwise the least value was revealed as 4,766.8±2,829.7 RLU after applying the used tip of interdental brush at the proximal area, by inserting into the CPC solution and shaking for a minute (p<0.01). 2. Average 9,475.6±368.2 RLU at proximal area was revealed before mouth rinsing with CPC solution, buta little bit decreased as 8,792.9±620.1 RLU, after a minute mouth rinsing with CPC solution (p<0.01). Conclusion: Based on the results, we concluded that disinfection and cleaning method by inserting the interdental brush tip in CPC solution and shaking for a minute, is clinically recommended to keep the tip clean after use at the proximal area.

Oral Manifestation and the Treatment of Self-Injurious Behaviors Associated with a Preterm Infant: A Case Report

Su-Hyun Shim,Byeng-Ken Cho,Ja-Won Cho 대한예방치과학회 2015 International Journal of Clinical Preventive Denti Vol.11 No.1

Behavioral problems could be easily observed in patients with various mental disorders. Self-injurious behavior (SIB) can be defined as deliberated harm to one’s own body without conscious suicidal intent. It usually occurs as head banging or hitting, body hitting, skin cutting, or finger biting. Also it frequently involves oral tissue’s damage in pediatric patients, such as lip, cheek and tongue biting. Various articles have described prostheses used to prevent SIB-related injuries to the oral tissues of patients who have developmental or medical problems, such as psychological problems, congenital syndromes or mental retardation. But reports of oral SIB in individuals unaffected by the above conditions are scarce. The case presented in this report discusses oral SIB in a premature baby. We found an optimal way that would prevent SIB-related injuries and also protect the causative teeth. These days, dentists should be able to manage those patients with several treatment modalities, furthermore, to prevent SIB according to the causative disorders