선모(仙茅) 열수(熱水) 추출물의 Collagen 유발 관절염에 대한 약리 효능 연구

서부일 ( Bu Il Seo ),노성수 ( Seong Soo Roh ),박지하 ( Ji Ha Park ),박찬익 ( Chan Ik Park ),구진숙 ( Jin Suk Koo ) 대한본초학회 2016 大韓本草學會誌 Vol.31 No.4

Objectives:In Korean medicine, Curculiginis Rhizoma was treated for arthritis in remedy. But efficacy of Curculiginis Rhizoma on collagen induced arthritis was not revealed. Methods:Anti inflammatory effect of Curculiginis Rhizoma was researched in vitro with RAW264.7 cell and cell toxicity, levels of proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-12) and PGE2 were analyzed by ELISA assay. Inflammatory protein were analyzed by western blotting assay (JNK, ERK, COX-2, TNF-α and IL-1β). In vivo, collagen induced arthritis mice model was used to evaluate anti-inflammation effect through arthritis index, immune cell number and cytokine levels (TNF-α, IL-6 and IL-1β) in serum. Results:ECR(Extract of Curculiginis Rhizoma) has not shown cell toxicity in 200 ㎍/㎖ on RAW264.7 cell. ECR suppressed releases of NO, TNF-α, IL-1β, IL-6, IL-12 and PGE2 on RAW264.7 cell treated with lipopolysacharide (1 ㎍/㎖). And ECR inhibited regulation of TNF-α, IL-1β and IL-6 mRNA, reduced protein release of JNK, ERK, iNOS, COX-2, IL-1β and TNF-α. AI of group treated with ECR 200 ㎎/㎏ and 100 ㎎/㎏ were significantly decreased compared to vihicle arthritis mice, the number of immune cell in foot joint was increased on control mice but those of group treated with ECR 200 ㎎/㎏ and 100 ㎎/㎏ were significantly reduced. This results correspond with contens of cytokines (TNF-α, IL-1β and IL-6) in serum. Conclusions:Curculiginis Rhizoma has anti-inflammation effect on RAW264.7 cell in vitro and collagen induced arthritis in vivo . So it is necessary to research more mechanism for cascade imfact.

Monosodium Iodoacetate로 유발한 골관절염 흰쥐에 대한 여지초(荔枝草)의 효능 평가

이수화 ( Lee Soo Hwa ),서부일 ( Seo Bu-il ) 한약응용학회 2021 한약응용학회지 Vol.21 No.1

Salviae Plebeiae Herba (SP) contains a numberous chemical components and reported to exert various pharmacological activities such as antioxidant, anti-inflammatory, antimicrobial, analgesic, hypoglycemic, antiviral, anti-tumour, and hepatoprotective effects. Even though it has these various effects, its scientific proof remains uncertain the effect and underlying mechanism about joint damage. Accordingly, the present study was designed to evaluate the therapeutic effects and possible underlying mechanism of SP on osteoarthritis. OA models were induced via intra-joint injection of monosodium iodoacetate (MIA) 50 ㎕ with 80 ㎎/㎖ in rats. Excluding the normal group, OA-induced mice were divided into 4 groups (control, INDO, SPL, SPH). The drug concentrations were indomethacin 5 ㎎/㎏, SP 500 ㎎/㎏, and SP 1000 ㎎/㎏, and were administered orally once a day for 2 weeks. After drug supplementation, the effects of SP were measured with serum analysis, western blotting, and histopathological staining. SP administration improved hind limb overload and it led to relieve pain. Moreover, it suppressed oxidative related factors. The NF-κB inactivation though the inhibition of IκBα phosphorlyation significantly decreased both inflammatory mediators such as COX-2 and iNOS and cytokines such as TNF-α, IL-1β, and IL-6. Whereas, anti-inflammatory cytokines like IL-4 and IL-10 were shown to increase. In addition, SP administration exerted a protective effect on ECM by reducing the expression of collagen degradation factors including MMP-1, -3, -13 and by increasing TIMP-1. Taken together, SP may possess the effect of delaying the progression of osteoarthritis and protecting cartilage. Accordingly, the present study may provide a scientific basis the chondroprotective effect of SP on MIA induced osteoarthritis.

우리나라 韓藥典 編纂의 基礎作業을 위한 各國 藥典의 植物性 韓藥材 基原 探索(Ⅲ)

徐富一 동서의학사 2002 동서의학 Vol.27 No.1

夏枯草의 免疫調節作用 및 알레르기 低減化에 關한 硏究

徐富一,金先熙 대한본초학회 1997 大韓本草學會誌 Vol.12 No.1

The results of Prunellae Spica on immune response and anti-allergic reaction were as follows. 1. In Prunellae Spica group, hemagglutinin titer and hemolysin titer were increased in comparison with control group. But the results were not recognized as having significance. 2. The number of Plaque forming cells were increased in case of Prunellae Spica. But the results were not recognized as having significance. 3. The number of Rosette forming cells were increased in case of Prunellae Spica group, and the results were significant. 4. In experimentation of phagocytic activity in peritoneal exudate cells and monolymphocytus cells, Prunellae Spica group showed increase. But Prunellae Spica group in spleen cells showed decrease on the contrary. But these results were not recognized as having significance. 5. In Prunellae Spica group, results of PCA response were increased in case of 2 week, 4 week, 6 week on the contrary. 6. In experimentation on histamine contents, Prunellae Spica group showed derease at all agent contact. But these results were of no importance. From above these results, Prunellae Spica group showed good effects on immunoreaction. And Prunellae Spica group showed effects on anti-allergic reaction Ⅰ.

韓國産 紅花子와 中國産 紅花子가 鎭痛 , 消炎 및 關節炎에 미치는 效果

서부일,이은숙,박지하,김상찬,변부형,최호영 대한본초학회 2001 大韓本草學會誌 Vol.16 No.1

This experimental study was done to compare the efficacy of Korean and Chinese Carthami Semen on analgesic effect and remedy of inflammation and arthritis. The results were as follows; 1. In analgesic action on the writhing syndrome in mice induced by 0.7% acetic acid, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in analgesic action on the writhing syndrome in mice induced by Acetylcholine HC1, Korean and chinese Carthami Semen extract group showed significant decrease in comparison with control group. 2. In Anti-inflammatory effects, Chinese Carthami Semen extract group showed significant decrease at 150 and 180 minutes after inducing paw-edema in comparison with control group. 3. In the method of adjuvant arthritis, orally administered for 21 days, Korean Carthami Semen extract group showed significant inhibition of the hind paw edema after 1 day and 21 days. Whereas Chinese Carthami Semen extract group showed some reduction, but the results didn's show significant decrease. 4. In the level of leucocytes count, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in the level of total protein. CRP and glucose, Korean and Chinese Carthami Semen extract group didn't showed significant change in comparison with control group.

구자가 卵巢摘出로 誘發된 흰쥐의 骨多孔症에 미치는 影響 (2)

徐富一,金先熙,卞晟僖,金美麗 대한본초학회 1998 大韓本草學會誌 Vol.13 No.2

This study was performed to investigate the effects of Allii Tuberosi Semen(菲子) on osteoporosis after ovariectomy in rats. Control group received a daily po administration of normal saline for 4 weeks after oophorectomy(OVX). The other experimental group received a daily po administration of water extract of Allii Tuberosi Semen for 4 weeks after oophorectomy(OVX-ATS). Therefore, we measured levels of calcium, phosphorus, ash weights in femur. These results were as follows : 1. In the level of bone phosphorus, the result of OVX was 63.85±1.86㎎/㎗, but the result of OVX-ATS was 68.18±0.85㎎/㎗. But there was no statistical significance. 2. In the level of bone calcium, the result of OVX was 141.66±2.19㎎/㎗, but the result of OVX-ATS group was 164.82±3.74㎎/㎗. And the result of OVX-ATS showed significant increase in comparison with OVX. 3. In the level of bone ash weight, the result of OVX was 0.31±0.005(g), but the result of OVX-ATS was 0.33±0.002(g). And the result of OVX-ATS showed significant increase in comparison with OVX. The observations from these studies and the past studies(=A study on the effects of Allii Tuberosi Semen in ovariectomized osteoporosis of rats) suggest possible protective effect of Alli Tuberosi Semen water extract against bone loss in ovariectomized rats.

淸代 醫學者인 汪昻에 關한 考察

徐富一 동서의학사 1999 동서의학 Vol.24 No.2

南北韓에서 利用되는 韓藥材의 起源과 韓藥名에 關한 硏究

徐富一,李政祜 대한본초학회 1998 大韓本草學會誌 Vol.13 No.2

For the sake of promoting economic interchange by herbs of South and North Korea, we studies on origin and name of herbs in South and North Korea. At the first, we investigated name and origin of vegetability herbs recorded in 「the second part of The Korean Pharmacopeia(the fifth revision)」. Judging from the results of investigation through 「The Korean Pharmacopeia(the fifth revision)」 and 9 books studied in North Korea, there is a little difference between South Korea and North Korea in the origin of Crassihizomae Rhizoma, Saussureae Radix, Prunellae Spica, and Schizonepetae Herba. And names of herbs utilzed in North Korea were mentioned by the Korean alphabet Name, these herb names of North Korea differented from herb names of South Korea. Therefore, in our opinion we shoud work the origin and name of herbs in South and North Korea.

산사가 알콜 투여로 유발된 흰쥐의 고지혈증과 간 손상의 예방에 미치는 영향

서부일 대한본초학회 2005 大韓本草學會誌 Vol.20 No.1

Objectives : This study was performed to prove the preventive effect of water extracts from Crataegi Fructus on hyperlipiderma and liver damage induced by alcohol. Methods : Except for the normal group, we fed rat on 25% alcohol for 55 days. And Crataegi Fructus extract was administrated for the same period. We measured the serum component in rat's blood, body weight and weight of liver. Results : 1. At first, we observed preventive effects of Crataegi Fructus on hyperlipiderma induced by alcohol. Crataegi Fructus group showed significant decrease of total cholesterol and triglyceride in comparison with those of the control group. Crataegi Fructus group showed significant increase of HDL(high-density lipoprotein) cholesterol in comparison with those of the control group. Crataegi Fructus group showed significant increase of body weight in comparison with those of the control group in 4weeks and 8weeks. 2. At second, we observed preventive effects of Crataegi Fructus on liver damage induced by alcohol. Crataegi Fructus group showed significant decrease of GOT, GPT, ALP and LDH in comparison with those of the control group. Crataegi Fructus group showed significant increase of liver weight in comparison with those of the control group. Conclusions : Reviewing these experimental results, it appears that water extracts from Crataegi Fructus have pharmaceutical preventive efficacy on hyperlipiderma and liver damage induced by alcohol. Therefore further additional study should be conducted to elucidate in depth the pharmaceutical efficacy of these.

동의보감의 활석에 대한 지질학 및 한의약학적 고찰

이재영,서부일,황덕환,이인호,임용수 慶北大學校 師範大學 科學敎育硏究所 1999 科學敎育硏究誌 Vol.23 No.-

Talc has been used as mineral drugs in the oriental medicine to cure catharsis, dysentery, dry breast, poor micturition, chronic indigestion, etc. according to the 'Donguebogam' which had been published by Heo Jun in 1613. In the book there is a description of 'talc from Chungju is of good quality'. To investigate the description geologically and oriental medically, talc from Dongyang Talc Mine in the Chungju area was analysed and compared with that of Oriental Medical Hospital of Kyungsan University, which has MgO 31.65%, CaO 0.73%, SiO_(2) 60.04%, ignition loss 6.4% close to those of pure talc containing MgO 31.7%, SiO_(2) 63.5%, H_(2)O 4.8%. However, talc from Dongyang Mine with MgO 31.8%, CaO 0.51% and SiO_(2) 61.0% is closer to pure talc and contains less impurities (Fe_(2)O_(3) 0.8%). Ca and Fe may add medical effects to talc drug as in the case of actinolite. Formula (3) logα_(Mg)^(2+) = 11.89 - 2pH and (4) logα_(Mg^(2+)/α^(2)_(H)^(+)) = 6.6 - 1.33 logα_(H4SiO4) may be used as basic data to predict and examine soluble contents of talc drug for medical experiments.