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      • SCIESCOPUSKCI등재

        Intake, Digestibility In vivo, N Utilization and In sacco Dry Matter Degradability of Grass Silage Harvested at Three Stages of Maturity

        Vranic, Marina,Knezevic, Mladen,Perculija, Goran,Bosnjak, Kresimir,Leto, Josip Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.2

        The objective of this experiment was to study the effects of grass maturity at harvest on the nutritive value of grass silage (GS) in relation to voluntary intake, digestibility, nitrogen (N) utilization and in sacco dry matter (DM) degradability. Silage was cut from a sward dominated by orchardgrass (Dactylis glomerata L.) at the late-vegetative (early-cut), internode elongation (mediumcut) and flowering (late-cut) stages of growth. The DM yield at harvest was the lowest for early-cut silage (5.4 t/ha) and increased to 6.5 and 7.0 t/ha for the medium and late-cut silage respectively. As the crop matured, the crude protein (CP) concentration decreased significantly (p<0.05) and there was a marked increase in acid detergent fiber (ADF) concentration (p<0.001). The three different silages were offered to four 18-month old Charolais wether sheep to measure the voluntary intake, in vivo digestibility and N retention over four 21-day periods in an incomplete changeover design. Silage degradability characteristics were determined using four fistulated sheep to measure DM degradability over 3, 6, 12, 24, 48 and 72 h. There was a linear decrease in the voluntary intake of silage fresh matter, DM, organic matter (OM) and neutral detergent fiber (NDF), digestibility of DM, OM, NDF, ADF and CP, and digestibility of OM in DM (Dvalue) ($P_L<0.01$) as harvesting of grass was delayed. Nitrogen intake, N output in urine, N output in faeces and N balance also linearly decreased ($P_L<0.01$) with postponed harvesting of grass for silage. DM degradability and effective degradability (ED) significantly decreased with increasing maturity of grass at harvest. The results suggest that harvesting date has a significant influence on the nutritive value of GS in terms of intake, digestibility, N balance and in sacco degradability in the rumen. It was concluded that early harvest GS ensured higher intake, digestibility, N intake and DM degradability in comparison with the medium and the late cut GS as a result of improved rumen N efficiency and utilization probably due to a better balance of available energy and protein.

      • KCI등재

        Efficacy of electrical neuromuscular stimulation in the treatment of chronic periodontitis

        Ivan Puhar,Amalija Kapudija,Adrian Kasaj,Gregory-George Zafiropoulos,Andrija Bosnjak,Darije Plancak,Brita Willershausen 대한치주과학회 2011 Journal of Periodontal & Implant Science Vol.41 No.3

        Purpose: The purpose of the present randomized controlled clinical study was to evaluate the short-term outcomes of microcurrent electrical neuromuscular stimulation (MENS) as an adjunct method to nonsurgical periodontal therapy. Methods: Twenty patients with moderate to severe chronic periodontitis were recruited into the study and randomly treated with either nonsurgical periodontal therapy followed by 5 MENS treatments with a microcurrent device or by nonsurgical periodontal therapy alone. Periodontal parameters were measured at baseline and 6 weeks following therapy, and included the plaque index, bleeding on probing, probing depth, and clinical attachment level (CAL). Results: All measured values were reduced at the time of re-evaluation. The amount of inflammation was significantly reduced in both the test (P=0.002) and control group (P=0.015). The test group demonstrated a significant CAL gain at 6 weeks following therapy, including non-molar (P=0.009) and molar teeth (P=0.028). In comparison with the control group, the test group showed statistically significant differences in the CAL on both molar (P=0.027) and non-molar teeth (P=0.031). Conclusions: In conclusion, the short-term results of the study indicate that MENS could be a suitable adjunctive method in the treatment of chronic periodontitis.

      • SCIESCOPUSKCI등재

        Efficacy of electrical neuromuscular stimulation in the treatment of chronic periodontitis

        Puhar, Ivan,Kapudija, Amalija,Kasaj, Adrian,Willershausen, Brita,Zafiropoulos, Gregory-George,Bosnjak, Andrija,Plancak, Darije Korean Academy of Periodontology 2011 Journal of Periodontal & Implant Science Vol.41 No.3

        Purpose: The purpose of the present randomized controlled clinical study was to evaluate the short-term outcomes of micro-current electrical neuromuscular stimulation (MENS) as an adjunct method to nonsurgical periodontal therapy. Methods: Twenty patients with moderate to severe chronic periodontitis were recruited into the study and randomly treated with either nonsurgical periodontal therapy followed by 5 MENS treatments with a micro current device or by nonsurgical periodontal therapy alone. Periodontal parameters were measured at baseline and 6 weeks following therapy, and included the plaque index, bleeding on probing, probing depth, and clinical attachment level (CAL). Results: All measured values were reduced at the time of re-evaluation. The amount of inflammation was significantly reduced in both the test (P=0.002) and control group (P=0.015). The test group demonstrated a significant CAL gain at 6 weeks following therapy, including non-molar (P=0.009) and molar teeth (P=0.028). In comparison with the control group, the test group showed statistically significant differences in the CAL on both molar (P=0.027) and non-molar teeth (P=0.031). Conclusions: In conclusion, the short-term results of the study indicate that MENS could be a suitable adjunctive method in the treatment of chronic periodontitis.

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