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배과수원에서 발생하는 차애모무늬잎말이나방(가칭, Adoxophyes sp.)에서의 성페로몬 합성 조절과 PBAN cDNA의 구조
부경생 서울대학교 농업개발연구소 2000 농업생명과학연구 Vol.4 No.-
The smaller tea tortrix, Adoxophyes sp. (Lepidoptera: Tortricidae) is a serious pest damaging leaves of pears and teas in Korea and other Asian countries. Adoxophyes in Asia including A. sp. have various characters in morphology, host preference, and sex pheromone composition. But the taxonomy, sexual isolation mechanism, and evolution of pheromone polymorphism in Adoxophyes are not clearly established. Though GC analysis, it was revealed that (Z)-9-tetradencenyl acetate (Z9-14Ac) and (Z)-11-tetradencenyl acetate (Z11-14Ac) are sex pheromone components of A. sp. Composition of pheromone components was 40 : 0 between z9-14Ac and Z11-14Ac in pheromone gland extract and female effluvia. Mating behavior and pheromone production in A. sp. Showed diel rhythm. A. sp. Mated and maximally produced the pheromone immediately after lights-on under a photoperiod of 16L/8D. Pheromone production was stimulated by injection of male of female head extracts, or Hez-PBAN. Therefore, it was proposed that pheromone production in A. sp. Is regulated by a neuropeptide, maybe PBAN, produced from the head. A 750 bp-long cDNA encoding PBAN of A. sp. Was fully characterized. PBAN cDNA contains a predicted open reading frame (ORF) of 576 nucleotides encoding and 192-amino acid long polypeptide. This polypeptide is predicted to be cleaved into 5 putative peptides including PBAN by endoproteolytic processing. PBAN of A. sp. Is a 31-aminoacid long neuropeptide and has 35-48% homology with PBANs from other moths. Other four putative peptides encoded in PBAN cDNA of A. sp. Are 24-amino acid, 7-amino acid, 20-amino acid, and 8-amino acid long. Through immunocytochemical studies, neuronal cells producing PBAN-like peptides were identified. Immunoreactive cells were located in the brain, suboesophageal ganglion (SG), and some ganglia in the ventral nerve cord (VNC). Immunoreactiv cells in the SG constitute three clusters and project neuritis to the corpora cardiaca. A couple of neuritis from the SG descended through the VNC and terminated in the terminal abdominal ganglion.
夫庚生 濟州大學校 亞熱帶農業硏究所 1984 亞熱帶農業硏究 Vol.1 No.-
We have nearly one million species of insect recorded in this earth. Moreover they pass through three or four different stages of development ; egg, larva, pupa and adult. All these mean that insects have evolved almost all means of physiological and ecological adaptation to their environment. Most of insect species live their own lives and about 15,000 species are directly or indirectly related to human lives, Among the latter group, most are beneficial, and only about 3,000 species are classified as harmful insects. We have tried to control insect pests with several methods, such as cultural, biological, mechanical, physical, legal and chemical method. The last has played the major role, with application of large quantities of pesticides, which result in three important ill effects ; 1. development of insect pests resistance to insecticides, 2. outbreaks of the pests due to destruction of their natural enemies, and 3. their potential health hazard to humans and wildlife. We need a lot of research and investment in developing alternative control methods in order to avoid those unwanted effects. There are various possibilities, but, in this paper, I discussed only two categories, physiological and genetic, which are already registered or have a good chance of future use. It would be much better if we could find some strategies based on physiological principles operating only in insects. Among this are included cuticle biosynthesis inhibitors, juvenile hormones and their analogues, anti-juvenile hormones, pheromones, kairomones, allomones, antimetabolites, and antifeedants. These chemicals are expected not to give so much troubles to man. Genetic methods can be employed to suppress or eradicate pest populations or to introduce genes innocuous to man into natural populations. This can be accomplished by sterile insect release, chromosome translocations, hybrid sterility, cytoplasmic incompatibility, compound chromosomes, meiotic drive, negative heterosis, conditional lethal mutations, etc. However, it must be emphasized that insect pest problems can be overcome only by integrated pest management programs. We have to select and wisely use the best mean(s) of pest control under a given condition.
부경생 서울대학교 농업개발연구소 1999 농업생명과학연구 Vol.3 No.-
Chrysopa cognata (Neuroptera: Chrysopidae) is the most important lacewing predator against aphids in Korea. In our research C. cognata was shown to respond to aphid sex pheromone chemicals,(-)-(1R,4aS,7S,7aR)-nepetalactol and (+)-(4aS,7S,7aR)-nepetalactone, in EAG measurements and olfactory bioassay experiments. And C. cognata were attracted in trapping at a chestnut orchard, an arboretum and a college campus where insecticides were rarely sprayed. C. cognata significantly more responds to nepetalactol than to nepetalactone, which was again significantly more effective than a solvent, hexane. They were caught in traps mainly during nights, especially from 7.30PM to 1.00AM in July and August in Suwon, Korea. But trap color did not influence the trapping efficiency. The number of C. cognata caught increased with the amount of the chemicals, up to 30mg per a lure, the highest amount tested in these series of experiments. Their catch extended throughout the season, from May to October, with more numbers in July and August. For a dispenser for aphid pheromone chemicals, rubber septa were much more effective than glass vials, in terms of longevity and attractivity. Another surprising result was that only males were caught in the traps. Besides this species a few individuals of male Chrysopa Formosa and Chrysopa phyllochroma were also caught in the pheromone traps.
양경부,조경호 제주대학교 공과대학 첨단기술연구소 2007 尖端技術硏究所論文集 Vol.18 No.-
An offset loop of profiles in sheet metal part is a tool paths for profile-machining, Offsetting is one of the most geometric problem in case of complex shape. In this paper we describe a method for generating NC tool paths for aerospace routing process using path trimming based on pair-wise intersection.
위암환자의 위액단백과 Amylase의 분리와 성상에 관한 연구
정부근,고재경,노연희 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.1
Amylase activity was measure din gastric juice and cancer tissue of patients with stomach cancer to find out the possible use of the enzyme as a biochemical marker for stomach cancer. Amylase and proteins in gastric juice from patients with stomach cancer were separated by a DEAE-cellulose column chromatography to investigate the enzyme and proteins specific to the stomach cancer. (1)Protein content and amylase activity were not changed in gastric juice of patients with gastritis and duodenal ulcer, but significantly increased in gastric juice of patients with stomach cancer. The positive rate of protein content and the enzyme activity in the gastric juice as a marker for stomach cancer was relatively high. (2)Protein content in the stomach cancer tissue was significantly increased and amylase activity was greatly increased in 3 cases out of 12 cases of the stomach cancer tissues studied. (3)Substrate specificity and effect of halogen ions on amylase in gastric juice of patient with the stomach cancer was similar to those on the enzyme in the stomach cancer tissue. (4)DEAE-cellulose columkn chromatography revealed that proteins in gastric juice were separated into 8 peaks in the stomach cancer and into 6 peaks in the control. Amylase in the gastric juice was separated by the chromatography into 2 isozymes in the stomach cancer, but not in the control. Substrate specificity for the two gastric juice amylase isozymes was observed to be different. These results indicate that protein content and amylase activity in the gastric juice could be used as a biochemical marker for the stomach cancer and that amylases specific to the stomach cancer appear to be released from the stomach cancer tissue.
이부수,김영식,이강현,황성오,임경수,박금수,윤정한,안무업,최경훈 대한응급의학회 1994 대한응급의학회지 Vol.5 No.2
Background : Mechanism of blood flow during cardiopulmonary resuscitation(CPR) in humans remains controversial and poorly understood, although cardiac or thoracic pump theory was proposed. We investigated cardiac movement, ventricular function and atrioventricular valve motion with aid of transesophageal echocardiography during precordial compression during CPR in humans. Methods and results : During CPR transesophageal echocardiography was performed in 14 patients with non-traumatic cardiac arrest. Manual precordial compression during CPR was performed according to American Heart Association guidelines. Mitral valve closed in 9 and did not close in 5 patients during "compression systole". Tricuspid valve closed during compression systole. Compression vector directed to right ventricle, basal portion of interventricular septum and left atrium. The heart rotated clockwise and the apex was more displaced than the base("swing motion"). Fractional shortening(FS) and ejection fraction(EF) of right ventricle exceeded those of left ventricle(FS : 55±9% vs 18±8%, p<0.05), EF : 79±9% vs 37±16%, p<0.05). FS and EF of left ventricle was higher in patients with systolic mitral valve closure than patients with persistent systolic opening of mitral valve(FS : 21±7 vs 13±7%, EF : 45±12 vs 22±12%, p<0.05), but FS and EF of right ventricle was not different. Conclusion : During precordial compression, the heart rotated clockwise and displaced. Systolic function of right ventricle exceeded left ventricle. Marked compression of right ventricle and systolic closure of tricuspid valve suggested that right ventricle functioned as a pump generating blood flow during precordial compression. Closure of mitral valve was dependant on systolic function of the left ventricle.
정경권,정성부,엄기환 동국대학교 산업기술환경대학원 1998 산업기술논총 Vol.4 No.-
In this paper, we propose a nonlinear system identification method using genetic algorithm. We represent the nonlinear system as a parameter vector and a measurement vector. In order to identify the nonlinear system, we find the parameter vector using genetic algorithm. The parameter vector is regarded as a chromosome of gene. The error between the desired output and estimated output every sampling period is used to calculate the fitness of one gene. The simulation results showed the effectiveness of using the genetic algorithm in the nonlinear system identification.
전도성 고분자 센서 어레이를 이용한 휘발성 유기 화합물 가스 인식
이경문,주병수,유준부,황하룡,이병수,이덕동,변형기,허증수 한국센서학회 2002 센서학회지 Vol.11 No.5
휘발성 유기 화합물 가스(Volatile Organic Compounds)를 인식하고 분석하기 위하여 전도성 고분자 센서어레이를 이용한 시스템을 제작하였다. Polypyrrole와 Polyaniline을 화학중합법으로 센서에 전도성고분자막을 형성하였고 이를 통해 VOC 검지용 센서 어레이를 제작하였다. 센서어레이로부터 측정되는 다차원 데이터는 주성분분석법(PCA)과 RBF(Radial Basis Function Network)을 이용하였다. 제안된 시스템으로 VOCs 가스를 인식하는데 있어서 RBF Network이 PCA방식보다 더욱 효율적인 것으로 판단되었다. We fabricated gas recognition system using conducting polymer sensor array for recognizing and analyzing VOCs(Volatile Organic Compounds) gases. The polypyrrole and polyaniline thin film sensors which were made by chemical polymerization were employed to detect VOCs. The multi-dimensional sensor signals obtained from the sensor array were analyzed using PCA(principal component analysis) technique and RBF(radial basis function) Network. Throughout the experimental trails, we confirmed that RBF Network is effective than PCA technique in identifying VOCs.
조부경 배화여자대학 1987 培花論叢 Vol.6 No.-
This study is to investigate the problems in infant-parent attachment relationship, based on the related literature. First, the concepts of attachment and attachment behavior are defined. Second, the objects of attachment for infant and the influential factors on infant-parent attachment relationship-parental behavior pattern, infant characteristics, social contexts, and duration of contact just after birth are examined. Next, the level of stability in attachment pat-terns, and the last the relationship between patterns of attachment and social competency are considered. The results obtained from this theoretical review are as follows: 1. According to Bowlby and Ainsworth's concept of attachment, attachment refers to the affectional tie that infant forms to his mother. And attachment behavior is the behavior that promotes proximity to or contact with his mother to be attached. 2. The objects of attachment for infant seem to be not only his mother but also his father. Parental affectionate behaviors that are responsiveness, hugging and cuddling more, have an important effect on the security of attachment. In addition, infant characteristics and social support are influential factors. Mother-infant contact in the first few hours of life is not considered critical effect on the development of secure attachment. 3. The secure attachment pattern during infancy tends to be stable care-giving contexts and optimal parent-infant interaction. 4. There is a little significant relationship between attachment pattern and social competency.