Fermentation Conditions for the Production of Cell Mass in Streptococcus faecalis and Endospores in Bacillus mesentericus

현형환,김부연,류한봉 한국외국어대학교 외국학종합연구센터 부설 기초과학연구소 1996 기초과학연구 Vol.4 No.-

Streptococcus faecalis는 glucose, lactose등을 탄소원으로 잘 이용하였으며 glucose를 탄소원으로, yeast extract와 casitone을 질소원으로 구성된 배지를 사용하여 pH 7.0에서 control되는 fermentor 배양에서 optical density(660nm) 7.3(viable cell concentration 6.5×10(9) cells/㎖)이상의 높은 cell concentration을 얻을 수 있었다. Bacillus mesentericus의 sporulation을 최대화 하기 위한 발효 조건을 조사한 결과 glucose를 위한 최적 CaCl₂농도는 0.lmM이었다. Streptococcus faecalis rapidly fermented glucose and lactose as a C-source, and the maximum cell concentration was obtained when grown in a jar fermentor that contained YCG medium consisting of glucose as a C-source and casitone and yeast extracts as a N-source, and that was controlled at pH 7.0. From experiments to maximize the sporulation efficiency in Bacillus mesentericus, glucose caused the decrease in sporulation efficiency, and calcium chloride was stimulatory for sporulation, of which optimum concentration was 0.lmM.

신경회로망에서 뉴런간의 연결도를 이용한 4백 배치 문제 해결 알고리즘

현해진,김덕주,강부식,이기태,임재윤 濟州大學校 工科大學 産業技術硏究所 1993 産業技術硏究報告 Vol.4 No.-

In this paper we have proposed a method for solving a four-coloring problem using adjusted information transmission between adjacent neurons in neural networks. These algorithms are based on McCclloch-Pitts binary neuron model neural networks. If a map shares n regions, a 4×n neural array is used to color a map of n regions where each neuron as a processing element performs it. By programming these algorithm by a C-language in IBM PC 486 DX and adapting it to various examples, we have showed the ability of our system. If one neuron is selected among four neurons, the strength of order three neurons is wakened. So, by transmitting the information of selected neuron, adjacent neurons cannot share the same color.

돌발성 난청의 임상적 분석

남부현,강갑진,성열웅,송영복 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

Sudden hearing loss is an abrupt onset of sensorineural hearing loss over a period of hours or a few days without definite cause. The cause and its pathophysiology have not been fully determined, and the treatmnet is controversial at present. The purpose of this study is to seek the best modality of treatment by clinical and stastical analysis of 48 patients (28 male and 20 female) diagnosed as a sudden hearing loss from January 1993 to October 1995. The therapeutic results of the 48 patients were 20 cases (41.7%) of complete recovery, 3 cases (6.2%) of partial recovery, 4 cases (8.3%) of slight recovery and 21 cases (43.8%) of no improvement. Early treatment within a week of onset, ascending or flat types of audiogram, and normal ABR were favorable prognostic factors in recovery of hearing. The presence of accompanying vestibular symptom (ie, vertigo) and profound hearing loss on initial audiogram were poor prognostic factors. However, the gender and the age of the patients did not affect on the hearing result.

후외상성 진주종 2례

남부현,김병국,고필준,심장한,박찬일 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.1

Post-traumatic cholesteatoma can be originated in the middle ear or mastoid cavity after head trauma such as temporal bone fracture, penetrating injury of the temporal bone or blast injury of the tympanic membrane. It usually takes a long time to develope cholesteatoma after injury, and physicians may overlook its association with previous, trauma. Post-traumatic cholesteatoma can be suspected in patients with head trauma history and atypical cholesteatoma findings. We experienced two cases of cholesteatoma which found behind the intact tympanic membrane in 7 and 13 years old boys with history of head trauma, and we are reporting this with a discussion and literatural review.

심폐소생술중 심실기능 및 판막운동의 평가

이부수,김영식,이강현,황성오,임경수,박금수,윤정한,안무업,최경훈 대한응급의학회 1994 대한응급의학회지 Vol.5 No.2

Background : Mechanism of blood flow during cardiopulmonary resuscitation(CPR) in humans remains controversial and poorly understood, although cardiac or thoracic pump theory was proposed. We investigated cardiac movement, ventricular function and atrioventricular valve motion with aid of transesophageal echocardiography during precordial compression during CPR in humans. Methods and results : During CPR transesophageal echocardiography was performed in 14 patients with non-traumatic cardiac arrest. Manual precordial compression during CPR was performed according to American Heart Association guidelines. Mitral valve closed in 9 and did not close in 5 patients during "compression systole". Tricuspid valve closed during compression systole. Compression vector directed to right ventricle, basal portion of interventricular septum and left atrium. The heart rotated clockwise and the apex was more displaced than the base("swing motion"). Fractional shortening(FS) and ejection fraction(EF) of right ventricle exceeded those of left ventricle(FS : 55±9% vs 18±8%, p<0.05), EF : 79±9% vs 37±16%, p<0.05). FS and EF of left ventricle was higher in patients with systolic mitral valve closure than patients with persistent systolic opening of mitral valve(FS : 21±7 vs 13±7%, EF : 45±12 vs 22±12%, p<0.05), but FS and EF of right ventricle was not different. Conclusion : During precordial compression, the heart rotated clockwise and displaced. Systolic function of right ventricle exceeded left ventricle. Marked compression of right ventricle and systolic closure of tricuspid valve suggested that right ventricle functioned as a pump generating blood flow during precordial compression. Closure of mitral valve was dependant on systolic function of the left ventricle.

미로누공에 대한 임상적 고찰

남부현,백승숙 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

15 cases of labyrinthine fistula operated by one junior staff from November, 1991 to November, 1993 were discussed. 14 cases were caused by cholesteatoma, and one case was caused by an iatrogenic surgical injury during otoneurologic procedure(petrosal approach). Clinical symptoms were mainly that of complication i.e., serous or suppurative labyrinthitis, facial palsy, brain abscess, meningitis, mastoid fistula and subperiosteal abscess. Temporal bone CT scan showed 100% sensitivity in detecting the lateral semicircular canal erosion, but fistula test proved less useful due to labyrinthine symptoms and high false nagative rate. Postoperative hearing results were more related to the method of fistula repair rather than the size of fistula, presence of perilymph leakage or inadverent perilymph aspiration during matrix removal. A canal plugging procedure, which was helpful in preservation of cochlea function in some cases, may prevent permanent intralabyrinthine fistula formation, and we suggest it as a method in treatment of selected cases of labyrinthine fistula.

전류측정에 의한 AC서보모터의 언밸런스 고장검출

송명현,조병호,김부열 순천대학교 2000 순천대학교 자연과학논문집 Vol.19 No.1

Using current measurement, the analytic method for the unbalance fault detection of 3Φ induction servomotor was proposed. The fault detection of the load unbalance has been studied by means of the measure of vibration. In the conventional method, the additional vibration sensors are set up to measure the vibration properly, but the method introduced in this paper, a current sensor for motor control is used for measuring the load current, so there is no need for additional sensors. After measuring the current caused by unbalance load, the FFT spectrum of the measared current were analyzed and compared with the spectrum pattern of the balanced load. Varying the motor speed by 500, 1000, 1500 and 2000 rpm, some characteristic frequency for detecting the unbalance fault were found. These experimental results show that the proposed current measuring method is more simple, easy, and effective than the conventional vibration measuring method.

Foot Index에 의한 발의 형태 분류 : 20대 여자 대학생을 중심으로

심부자,유현 동아대학교 생활과학연구소 2001 생활과학연구 논문집 Vol.9 No.-

The purpose of this study was to analyze the characteristics of women's foot and foot shape by foot index. The sample size for this study were 83 college Women of the age of 20's and Data were measured with the direct measurement method and indirect measurement method. The results were as follows : 1.Foot shape were grouped with narrow foot shape group (14.4%), intermediate foot shape group (67.5%), and wide foot shape group (18.1%). 2.After overviewing the characteristics of foot shape among individual foot shape group, significant results were obtained at several items ; Heel - instep girth, instep girth, medial malleolus height, lateral malleolus height, instep height, foot width, foot length, foot length of the first toe , foot length of the fifth toe. 3.The size difference between wearing shoes and bare foot of all participant were variable. Participants of 34.9% showed 5mm of size difference, participants of 57.8% showed 5-15mm of size difference, participants of 7.2% showed more than 15mm of size difference. 4.Participants were examined foot skin damages caused by wearing shoes. Participants of 49% had no foot skin damages, and participants of 51% had one or more foot skin damages.

淳于意의 診籍에 나타난 疾病의 考察

金富煥,朴炫局 동국대학교 한의학연구소 1995 東國韓醫學硏究所論文集 Vol.4 No.-

The aim of this study is to compare the diseases which are in the Sun Woo-Yee」s charts with the diseases which are in the medical books excavated in MA WANG TEI(馬王堆), 「YOUNG COO(靈樞)」 and 「So MUN(素問)」, and to investigate their interactions. The results obtained are as follows : 1. JOE(疽) which is made in stomach and bowels is not found at the medical books excavated in MA WANG THI(馬王堆), 「YOUNG COO(靈樞)」 and 「SO MUN(素問)」, JOE(疽) which is made on the breast similar to JOE(疽) which is made on the chest of 「YOUNG COO(靈樞)」. 2. It is said in Sun Woo-Yee(淳于意)'s charts that mental depression blocks up the circulation of Kl(氣), and it is the same viewpoint of 「YOUNG COO(靈樞)」and 「SO MUN(紫間)」. 3. The POONG GYUL(風??) and the YOUL GYUL(熱??) that are found in the Sun Woo-yee(淳于意)'s charts is similar to those of 「SO MUN(素問)」, but different from those of「YOUNG COO(靈樞)」. 4. It is regarded that YOUNG SAN(湧疝) in the Sun Woo-Yee(淳干意)'s charts is similar to the COONG SAN(??疝) in 「SO MUN(索問)」, KI SAN(氣疝) in the Sun Woo-Yee(淳于意)'s charts is related to the TUI SAN(癲疝) of the medical books excavated in MA WANG TUI(馬王堆), 「YOUNG COO(靈樞)」 and 「SO MUN(素閒)」, MO SAN(牡癌) in the Sun Woo-Yee(淳于意)'s charts is considered PE SAN(肺疝) and PE POONG SAN(肺風疝) in 「SO MUN(素問)」, but more and deeper study is required. In the change of the terms : from TUI SAN(癲疝) to TUl SAN(癲疝), from PYUN SAN(偏疝) to H0 SAN(狐疝), it can be guessed that the terms changed, as new doctrines were introduced and reorganiged with the development of the medical theory. 5. POONG BI(風痺) in the Sun Woo-Yee(淳于意)'s charts is not found in 「YOUNG COO(靈樞)」, but it is similar to DAN FEVER(??熱) in 「So MUN(素問)」. It is regarded that PE SO DAN(肺消癲) in the Sun Woe-Yee(淳于意)'s charts is the same as that of 「YOUNG COO(靈樞)」. 6. DONG PUNG(동風) in Sun Woo-Yee(淳于意)'s charts is not found in 「YOUNG COO(靈樞)」and 「SO MUN(素問)」, but it seems like DONG SOEL(洞泄) in 「YOUNG COO(靈櫃)」. It is regarded that the DONG PUNG(동風)』 in the Sun Woo-Yee(淳于意)'s charts was at first changed into DONG SOEL(洞泄), and later differentiated into DONG SOEL(洞泄) and SON SOEL(손泄). 7. In the Sun Woo-Yee(淳子意)'a charts, the treatment of the decayed tooth had the classification of the right and left, seeing cauterization with moxa on SU YANG MYOUNG MAEK(手陽明脈), it is considered that the tooth was decayed in the lower right region. A tooth was related to QI MAEK(齒脈) and BI YANG MYUNG MAEK(臂陽明脈) in the early stage, but gradually was related to not only SU YANG MYUNG MAEK(手陽明脈) but also JOK YANG MYUNG MAEK(足陽明脈), JOK SO YIN MAEK(足少陰脈), JOK TAE YIN MAEK(足太陰脈), and in regards to the tooth and KYUNG MAEK(經脈), 「YOUNG COO(靈樞)」 emphasised the course of the KYUNG MAEK(經脈), 「SO MUN(素問)」 emphasised the attachment of the five elements. 8. In regards to BI(痺) , the importance of the five elements thee given in the Sun Woo-yee(淳于意)'s charts is similar to that of 「SO MUN(素問)」, and 「YOUNG COO(靈樞)」 and the medical books excavated in MA WANG TUI(馬王堆) give the same importance to the KYUNG MAEK(經脈) theory. 9. The explanation of the irregular menstruation in the Sun Woo-Yee(淳于意)'s charts was fundamentally similar to that of 「SO MUN(素問)」, but it shows the another theory that can not found in 「SO MUN(素問)」.

SILICONE TUBE에 삽입된 신경절편이 말초신경 결손부의 재생에 미치는 효과

장현중,박노부,홍창수 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.4

The purpose of this study is to observe the stimulant effect of the isolated nerve segment on the peripheral nerve regeneration using silicone tube in rats. Srague-Dawley female albino rats were used as experimental animals and nerve defects were made by resection of 14 mm-long segment of right sciatic nerves. In control group, the defects were bridged with the silicone tubes. In experimental group, the defects were bridged with the same manner and additionally, 2mm-long nerve segments were inserted at the center of the silicone tubes. 3 months later, the regenerated nerve tissue within the silicone tubes were examined by histologic study. Axonal diameters and numbers of axons were measured and all data were analyzed by using SAS package program. The results obtained were as follows : 1. The experimental group was increased than the control group in general diameters of the regenerated nerve fiber.(P<0.05) 2. The diameters of axons were increased int he experimental group compare to the control group. (P<0.05) 3. The numbers of axons were increased int he experimental group compare to the control group. (P<0.05)