Lard와 Alcohol을 攝取한 흰쥐血淸中의 脂質成分에 마늘이 미치는 影響

변부형 慶山大學校 1995 論文集 Vol.13 No.1

The present study has been undertaken to investigate the effects of garlic added to food on the lipid composition in serum of rats fed lard and alcohol. Thirty-five males of Sprague-Dawley strains weighed about 130g were divided into 7 groups, each group receiving a different diet for 10 weeks ; i.e. basal diet plus 15% lard, basal diet plus 5% alcohol, basal diet plus 0.5% garlic, basal diet plus 15% lard and 0.5% garlic. Determinations were carried out on the net weight gain, food efficiency ratio, weight of organs, triglyceride, total cholesterol, HDL-cholesterol, free cholesterol. The results obtained were as follows : 1. Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but garlic caused an increase in food efficiency ratio. 2. Lard supplementation caused an increase in the weight of liver, kidney, spleen, but another groups did not. 3. The contents ratio of triglyceride, total cholesterol in serum of lard-fed group showed significantly increase compared to controls and but the simultaneous Supple-mentation of garlic and lard decreased significantly than those of lard-fed group. 4. The contents of free-cholesterol and ester cholesterol in serum of the simultaneous supplementation of garlic decreased as compared with the lard single fed group. 5. Garlic single-fed group increased plasma HDL-cholesterol compared to controls but in any other groups were not changed. 6. The ratio of contents of VLDL, LDL-cholesterol and HDL-cholesterol in serum was significantly high in the only lard containing group and alcohol group, the garlic supplemented group was showed lower tendency than only lard and alcohol group. The above results suggest that garlic would prevent the metabolic disease by improv-ing hyperlipemia.

V79 배양 세포에 있어 인삼추출물이 DNA복제 및 회복 합성에 미치는 분자생물학적 연구

卞富亨,徐富一,卞晟僖 대한본초학회 1999 大韓本草學會誌 Vol.14 No.2

본 연구는 인삼추출물이 DNA 복제 및 회복 합성에 미치는 영향을 조사하기 위해 수행되었다. DNA 복제 및 회복 합성도의 측정은 V79 chinese hamster lung cell의 핵 내 DNA에 [3H]-tymidine이 유입되는 양으로 측정하였다. 그 결과 인삼추출물의 농도(0~1ug/㎖)가 높을수록 점점 더 크게 감소하였다. 이와 같이 인삼추출물에 의하여 DNA 복제 합성도가 감소하는 현상은 농도가 더 높을수록 효과적인 것으로 보아 농도에 의존한다는 것을 알 수 있었다. 한편 DNA 회복 합성도에 인삼추출물이 미치는 영향을 알아보기 위해 우선 DNA를 손상시켰다. DNA를 손상시키는 물질은 여러 가지 있으나, 본 실험에서는 자외선과 methylmethane sulfonate(MMS)를 사용하였다. 이렇게 손상된 DNA가 인삼추출물(0~1ug/㎖)에 의하여 얼마나 회복되었는가를 알아보기 위하여 인삼추출물의 농도를 각각 달리하여 V79 cell에 첨가하였다. 그 결과 인삼추출물의 농도가 높을수록 DNA 회복합성도는 증가하였다. 이와 같이 인삼추출물이 DNA 복제 합성은 억제하고, DNA 회복 합성은 증가시키는 상반된 효과를 보여 주었다. 이러한 상반된 효과는 복제합성과 회복합성의 두 과정에서 서로 다른 효소체계가 관여하기 때문인 것으로 보인다. 인삼추출물이 DNA복제 및 회복합성에 미치는 영향에 대하여 보다 더 구체적인 분자생물학적 메커니즘 연구가 이루어져야 할 것이다.

마늘成分이 Lard와 Alcohol을 攝取한 흰쥐 血淸中의 酵素活性에 미치는 影響

변부형 慶山大學校 1994 論文集 Vol.12 No.1

The present study has been undertaken to investigate the effects of garlic added to food on the activities of several enzymes in serum of rats lard and alcohol. Thirty-five males of Sprague-Dawley stains weighed about 130 g were divided into 7groups, earth group receiving a different diet for 10 weeks ; i.e. basal diet plus 15% lard, basal diet plus 5% alcohol, basal diet plus 0.5 % garlic, basal diet plus 15% lard and 0.5% garlic. Determinations were carried out on the net weight gain, food efficiency ratio, weight of organs, and glutamic oxa1acetic transaminase, glutamic pyruvic transaminase, lactate dehydrogenase, alkaline phogphatase activities in serum of rats. The results obtained were as follows : 1. Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but garlic caused an increase in food efficiency ratio. 2. Lard supplementation caused an increase in the weight of liver, kidney, spleen, but another groups did not. 3. AST, ALT, ALP, LDH of serum were significantly increased in lard and alcohol containing group but garlic feeding decreased enzyme activities compared to lard and alcohol containing group.

Perm과 Coloring 처리에 의한 유아와 성인 모발의 손상

이부형 한국위생과학회 2002 한국위생과학회지 Vol.8 No.2

The morphological changes were investigated for the purpose of suggest the basic information to lesson hair damages of children(female) and women by the treatment of perm and coloring. perm and coloring were treated one time and tree times at a week's interval at different temperature(25℃, 50℃), respectively. The virgin hairs, which were not treated with perm and coloring were morphologically healthy. All of the hair cuticle strata were well settled. Children's perm hairs, which were treated one tine at 25 ℃, were relatively healthy though one or two spots were unsettled. On the occasion of perm hairs treated three times at 25 ℃, most of the hair cuticle strata were unsettled, and four or five spots were cracked. On the occasion of perm hairs treated one time at 50 ℃, one or two sports of the hair were cracked, and two or three spots were tom. On the occasion of perm hairs treated three times at 50 ℃, most of the hair cuticle strata were unsettled and cracked. The damages of women's hairs were much less than those of children's hairs. Women's perm hairs, Which were treated one time at 25 ℃, were mot damaged at all. On the occasion of perm hairs treated one time at 50 ℃, one or two spots of the hair were cracked and torn, but very clean. But, on the occasion of perm hairs treated three times at 50 ℃, many cuticle strata were cracked and were in pieces. The damages of children's coloring hairs, which were treated one time at 25 ℃, were very little. On the occasion of coloring hairs treated three tomes at 25℃, most of the hair cuticle strata were unsettled. On the occasion of coloring hairs treated three tines at 50 ℃, all of the hair cuticle strata unsettled and five of six spots were cracked, and four or five spots melted. After coloring like perm treatment, the damages of women's hairs were also much less than those of children's hairs.

Stearic acid, Oleic acid, Elaidic acid에 의한 간세포의 성장 및 지방산 조성의 변화

변부형 慶山大學校 1997 論文集 Vol.15 No.1

This study was carried out in order to investigate the effect of stearic acid (18:0,SA), oleic (18:1cis.0A) and elaidic acid (18:1 trans, EA) on the cell growth and fatty acid composition of Hep-G2 cells. The cells were incubated in serum-free medium 25, 50, 100 and 200uM of fatty acid combined with albumin for 2 days. The fatty acid concentration up to lOOuM showed the normal growth, but the cell growth decreased in the presence of 200uM fatty acid. The treatment of cells with lOOuM of a fatty acid for two days significantly (p<0.05) increased the cellular triglyceride (TG) content in all fatty acid groups compared to control, but TG content was not significantly different among all treatment group, but total cholesterol (TC) was the highest level in EA group. Cellular phospholipid(PL) contents were similar between the control and all faty acid groups.

고지방식이를 섭취시킨 흰쥐의 혈청 지질성분에 도라지가 미치는 영향

卞富亨,徐富一 대한본초학회 2001 大韓本草學會誌 Vol.16 No.2

Objects : This study was carried out to investigate the effects of Platycodi Radix powder on improvement of lipid composition in high fat-fed rats. Methods : Male sprague-Dawley rats of 7 weeks old, weighing 318.43±3.09g, were divided into two groups; the control group(C group: high fat(30%, Lard)) and platycodi Radix group(P group: 20% Platycodi Radix powder), they were fed experimental diets for 8 weeks. Results : Food intake and body weight gains had differences between the groups. Serum cholesterol levels were significantly lower in P group than C group. The level of serum HDL- Cholesterol was higher in P group than C group. The level of Serum LDL-C was significantly lower in P group than in C group. The ratio of HDC-Cholesterol to total Cholesterol were higher in P group than in the C group, too. The Value of atherogenic index(AI) was determined to be low in P group.

人蔘이 卵巢摘出로 誘發된 흰쥐의 骨多孔症 예방에 미치는 효능

정국영,서부일,이은숙,변부형,신순식,박지하 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1

Objectives : The present study has been undertaken to investigate the effects of Ginseng Radix on prevention of osteoporosis in ovariectomized rats. Methods : In this experiment, the rats were ovariectomized. Rats were administered by 2 kinds of medicine, Estradiol, Ginseng Radix. The levels of bone mineral density, osteocalcin, ALP, phosphorus, calcium in serum, deoxypyridinoline in urine, calcium, phosphorus, ash weight of bone, body weight and uterus weight were measured. Results : The levels of spinal bone mineral density was significantly increased in comparison with OVX group at 8 weeks in Ginseng Radix group. The levels of serum didn t show significant change in comparison with OVX group in Ginseng Radix group. The levels of urine deoxypyridinoline showed significant decrease in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial ash weight didn t show significant increase in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial calcium showed significant increase in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial phosphorus showed significant increase in comparison with OVX group in Ginseng Radix group. The levels of body weight didn t show significant changes in comparison with OVX group in Ginseng Radix group. The levels of uterus weight didn t show significant changes in comparison with OVX group in Ginseng Radix group. Conclusions : Reviewing these experimetal results, it appears that Ginseng Radix have efficacy on prevention of osteoporosis. And further study should be conducted to illustrate in depth the curing and prevention of osteoprosis.

부산광역시 일부하천의 계절적·년도별 수질특성 비교분석

정유진,문종익,성낙창,김부길,이영형 동아대학교 환경문제연구소 1999 硏究報告 Vol.22 No.1

This study is carried out to investigate the water quality change trends in Pusan Metropolitan streams. The temperature of the streams are moderate except in winter period and this can be explained by the Korean meterological characteristics. The pH does not vary by the time but some points are a little low. BOD_(5) and COD_(Mn), the index of water contaminants, be shown as characteristic domestic waste water patterns. The nutrient matters, such as total nitrogen and total phosphate, are appeared to be low. The chronic water quality, as BOD_(5) and COD_(Mn), shows that the loading rates in 1998 are smaller than the past(1983, 1992). Some points show a little high loading in autumn, but this needs more investigation to make clear the cause(s).

韓國産 紅花子와 中國産 紅花子가 鎭痛 , 消炎 및 關節炎에 미치는 效果

서부일,이은숙,박지하,김상찬,변부형,최호영 대한본초학회 2001 大韓本草學會誌 Vol.16 No.1

This experimental study was done to compare the efficacy of Korean and Chinese Carthami Semen on analgesic effect and remedy of inflammation and arthritis. The results were as follows; 1. In analgesic action on the writhing syndrome in mice induced by 0.7% acetic acid, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in analgesic action on the writhing syndrome in mice induced by Acetylcholine HC1, Korean and chinese Carthami Semen extract group showed significant decrease in comparison with control group. 2. In Anti-inflammatory effects, Chinese Carthami Semen extract group showed significant decrease at 150 and 180 minutes after inducing paw-edema in comparison with control group. 3. In the method of adjuvant arthritis, orally administered for 21 days, Korean Carthami Semen extract group showed significant inhibition of the hind paw edema after 1 day and 21 days. Whereas Chinese Carthami Semen extract group showed some reduction, but the results didn's show significant decrease. 4. In the level of leucocytes count, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in the level of total protein. CRP and glucose, Korean and Chinese Carthami Semen extract group didn't showed significant change in comparison with control group.

Single Dose Toxicity Study of Hwang-Guem Aqua-aqupuncture Solution

Boo-Hyeong Byun 한국생명과학회 2003 한국생명과학회 심포지움 Vol.40 No.-