PCR을 이용한 국내 및 수입 축산물 유래 Listeria monocytogenes의 유전학적 분석연구

우용구,이수화,이철현,최정수,류재두,김영일,이오수,김봉환 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2

One hundred-fifty two of Listeria strains were isolated from domestic chicken carcasses, a slaughter-houses from nationwide from 1996 to 1997 and imported livestock products from a ten foreign countries including the USA, China, France and Thailand. To substitute the laborious and time consuming procedures of clinical laboratory standard diagnostic methods, the rapid and specific multiplex PCR(M-PCR), which was designed to amplify a three kinds of genes simultaneously f3r specific detection and differentiation of L. monocytogenes(LM) by one step procedure, was established. The LM strains confirmed with M-PCR were analysed to their genetic diversities by RAPD using the two primers(D87 & MMTl), and also evaluated on their discriminatory abilities(na) between two primers. our study also conducted the REP-PCR and ERIC-PCR for evaluation of the possibility of REP and ERIC elements for genetic subtyping of LM strains. According to the REP-PCR and ERIC-PCR fingerprinting patterns, LM strains were divided into 7(ERIC-type) and 4(REP-type) major clusters at the relative clone cut off value of 80%. According to the computer analysis of PCR results using the GelCompar Ⅱ software, ERIC-PCR(DI=0.955) had expressed the most high discriminatory ability and followed by REP-PCR(DI=0.952), RAPD(D87; 0.954, MMT1; 0.937), in order. The combined analysis of the present PCR results was also expressed the reliable and useful discriminatory ability(DI=0.916). This study suggested that PCR based methods could be used as an reliable, reproducible, rapid, and highly discriminatory method for the genetic differentiation of genus Listeria.

진자검사와 동적근전도를 이용한 경직 평가

이은우,조강희,김봉옥 大韓再活醫學會 1999 Annals of Rehabilitation Medicine Vol.23 No.3

과호산구증가증의 원인과 발생율에 대한 임상적 고찰

김봉준,이천우,조은주,김희규 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Background: Eosinophilia is associated with various primary and reactive conditions. The incidence and causes of eosinophilia might have changed through time according to the changes in the incidence of diseases such as cancer, chronic degenerative diseases, and so forth. This study was sought to investigate the causes and the incidence of hypereosinophilia and compare the results with those obtained by other reports in Korea. Methods: Eosinophilia and hypereosinophilia are diagnosed when the absolute eosinophil count is greater than 500/μL and 1,500/μL, respectively. Patient's clinical records were reviewed to determine the underlying clinical conditions in relation to what causes hypereosinophilia. Results: Out of 18,941 patients who undertook hematology profiles, 1,584 (8.4%) and 143 (0.75%) were found to have eosinophilia and hypereosinophilia, respectively. Among patients with hypereosinophilia, 106 patients (74.1%) had identifiable and/or possible causes. The major causes of hypereosinophilia were malignancy (48.1%), allergy and skin diseases (22.6%), infectious diseases (9.4%), gastrointestinal tract diseases (5.7%), and hepatobiliary diseases (4.7%). Conclusion: We found various causes of hypereosinophilia in this study, and the most common cause of hypereosinophilia was malignancy. Therefore, a thorough study in patients with hypereosinophilia is necessary.

Cobb

이은우,임정수,손민균,김봉옥 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.4

[논문]결정화 유리첨가에 의한 BNT 계 세라믹 복합체의 소결 및 유전특성

이우석,이병철,류봉기 釜山大學校生産技術硏究所 2003 生産技術硏究所論文集 Vol.62 No.-

본 연구에서는 BaO-Nd2O3-TiO2계 마이크로파 유전체 세라믹스에 결정화 유리를 첨가하여 적충일체형 RF 수동소자 모률 구현을 위한 저온소결 유전체소재를 개발하고자 하였다. BaO-Nd2O3-TiO2에 결정화 유리를 5~3Owt% 범위로 첨가하여 소결온도를 1300°C 에서 1050 °C 로 낮추었으며, 첨가량의 증가에 따라서 소결밀도의 증가 및 입성장이 뚜렸이 일어났고 결정화 유리를 20wt% 첨가하여 1050 °C의 소결온도에서 95% 이상의 상대밀도를 갖는 소결체를 얻을 수 있었다. 이때 유전율(Er),72,품질계수(Q . f),1500, 공진주파수 온도계수(tf), 22 ppm/°C 을 나타내었으며 이는 LTCC 재료로서 응용가능성이 있다고 판단되었다.

Ti-6Al-4V의 저주기 고온염 피로균열성장거동에 관한 연구

우흥식,강봉수,김봉철 東國大學校 1999 東國論叢 Vol.38 No.-

Fatigue crack growth behaviour of Ti-alloy, Ti-6Al-4V were investigated in air and salt corrosive environments. The temperatures which was tested were room temperature and 200℃. Fatigue crack growth rate is known to be fast for formation of corrosive product by impregnation Ti-Alloy with salt solution in hot salt environment. For the effect on corrosion fatigue crack growth behabiour of sale corrosive transformation in air and high temperature of region Ⅱ, fatigue crack grwoth rate in air atmosphere had a little gap to both case, 200℃ and room temperature. However, it showed very fast tendency especially in salt corrosive atmosphere, and it was remarkably accelerated in 200℃ high temperature salt environment. When ΔK was approximately 30MPa√m, fatigue crack growth rate had a little difference between at room temperature and at 200℃ high temperature, however in case of salt corrosive environment, the former was 3.5 times faster and the latter for 16 times than air environment respectively. For salt corrosive environment of microstructure (Ⅲ), the fatigue life in 200℃ salt corrosive environment was shorter than that of in air, reached to only 32%.

Ti-50.1at.%Ni합금의 변태거동에 미치는 시효처리의 영향(Ⅰ)

우흥식,박성범,강봉수,김성진 한국안전학회 2004 한국안전학회지 Vol.19 No.4

This study investigated the effects of aging on the transformation behavior of Ti-50.lat.% Ni alloy by means of differential scanning calorimetry. It was found that aging in the temperature range of 350℃~550℃ induced complex transformation behavior, involving the R-phase and multiple-stage martensitic transformation. Usually aged Ni-rich NiTi alloys undergo martensitic transformation on cooling from high temperatures in two step : B2 to R and then R to B19' (normal behavior). But under certain ageing conditions, the transformation can also occur in three or more step(unusual multiple step behavior). In the present study we use differential scanning calorimetry(DSC) for a systematic investigation of the evolution of transformation behavior with ageing temperature and time.

薏苡仁의 投與가 마우스의 細胞性 및 體液性 免疫機能에 미치는 影響

우영은,김형균,송봉근,이언정 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

Coicis Semen is one of the oriental medicine that has been used for the treatment of the disease such as pulmonary abscess, periappendicular abscess and wart since ancient times. However, the mechanism of the action of the drug is not well studied. This study was done to investigate the effects of Coicis Semen on the host defense mechanism. Effects of Coicis Semen on the immune responses were analyzed by measuring the contact gypersensitivity, hemagglutinin, hemolysin and rosette formation, cytotoxicity, and reactive oxygen intermidiates production. As the results, water extract of Coicis Semen administration enhanced the antibodies (hemagglutinin and hemolysin) formation and the appearance of rosette forming cells of the spleen. Also Coicis Semen increased the allogeneic immune response in the mouse, showed cytotoxic activity against human leukemia cell line(K562) and decreased the contact hypersensitivity against dinitroflurobenzene. Also administration of Coicis Semen slightly increased NK cell activity and enhanced the production of such reactive oxygen intermidiates as superoxide and hydrogen peroxide from the macrophages in vivo and in vitro. The above results demonstrate that Coicis Semen has enhancing effects on cellular and humoral immune responses against disease.

신경망을 이용한 렌즈 왜곡 모델을 이용한 카메라 보정

우동민,김민석 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

A new camera calibration method based on a lens distortion model by an artificial neural network is proposed for three dimensional computer vision problems. The camera calibration procedure using the proposed method employs the widely used two step method. In the first step, the distortion-free linear camera model is constructed by estimating parameters of the model. In the second step, the proposed neural network model is established for mapping the distorted image points with the ideal image points. In order to show the performance of the proposed method, images from two different cameras with two different camera angles were used for calibrating the cameras. The performance of the proposed neural network approach is compared with the well-known Tsai's two stage method in terms of calibration errors. The results show that the proposed approach gives much more stable and acceptable calibration error over Tsai's two stage method regardless of camera resolution and camera angles.

오차를 최소화한 웨이블릿 영상 압축기의 하드웨어 설계

우광제,강봉순 동아대학교 정보기술연구소 2003 情報技術硏究所論文誌 Vol.11 No.1

In this paper, a hardware architecture of digital image compression' CODEC using ZD-DWT(Discrete Wavelet Transform) is designed. It used the Daubechies' (9,7) bi-orthogonal filter and included a 2D-DWT transformer, a quantizer. This paper proposes that final output has error rate within ±ILSB. Quantizer uses scalar quantization methods.