In vivo transduction of ETV2 improves cardiac function and induces vascular regeneration following myocardial infarction

Lee, Sunghun,Lee, Dong Hun,Park, Bong-Woo,Kim, Riyoun,Hoang, Anh Duc,Woo, Sang-Keun,Xiong, Wenjun,Lee, Yong Jin,Ban, Kiwon,Park, Hun-Jun Nature Publishing Group UK 2019 Experimental and molecular medicine Vol.51 No.2

<▼1><P>Vascular regeneration in ischemic hearts has been considered a target for new therapeutic strategies. It has been reported that ETV2 is essential for vascular development, injury-induced neovascularization and direct cell reprogramming of non-endothelial cells into endothelial cells. Thus, the objective of this study was to explore the therapeutic potential of ETV2 in murine models of myocardial infarction in vivo. Direct myocardial delivery of lentiviral ETV2 into rodents undergoing myocardial infarction dramatically upregulated the expression of markers for angiogenesis as well as anti-fibrosis and anti-inflammatory factors in vivo. Consistent with these findings, echocardiography showed significantly improved cardiac function in hearts with induced myocardial infarction upon ETV2 injection compared to that in the control virus-injected group as determined by enhanced ejection fraction and fractional shortening. In addition, ETV2-injected hearts were protected against massive fibrosis with a remarkable increase in capillary density. Interestingly, major fractions of capillaries were stained positive for ETV2. In addition, ECs infected with ETV2 showed enhanced proliferation, suggesting a direct role of ETV2 in vascular regeneration in diseased hearts. Furthermore, culture media from ETV2-overexpressing cardiac fibroblasts promoted endothelial cell migration based on scratch assay. Importantly, intramyocardial injection of the adeno-associated virus form of ETV2 into rat hearts with induced myocardial infarction designed for clinical applicability consistently resulted in significant augmentation of cardiac function. We provide compelling evidence that ETV2 has a robust effect on vascular regeneration and enhanced cardiac repair after myocardial infarction, highlighting a potential therapeutic function of ETV2 as an efficient means to treat failing hearts.</P></▼1><▼2><P><B>Cardiovascular disease: New hope for healing the heart</B></P><P>A gene therapy strategy that stimulates cardiovascular repair could improve recovery for heart attack patients. Heart attacks inflict severe damage on the heart and blood vessels, tissues with limited capacity for self-repair. Researchers led by Kiwon Ban of the City University of Hong Kong and Hun-Jun Park of the Catholic University of Korea, Seoul, have now demonstrated that a gene responsible for cardiovascular development can also efficiently stimulate heart repair. They used viruses to deliver the gene into a mouse model of heart attack, and showed that treated heart tissues exhibited strong recovery relative to untreated controls. The treatment reduced scar tissue formation and promoted proliferation of the cells lining blood vessels and blood vessel formation, measurably improving heart function. This approach could lay the groundwork for treating a common potentially fatal event.</P></▼2>

표고골목 해균인 주홍꼬리버섯을 방제하기 위한 저항성 표고균주 선발

이봉훈 외 한국버섯학회 2005 한국버섯학회지 Vol.3 No.3

Attempts were made to control Diatrype stigma occurred on the bed-log of shiitake by resistant shiitake strains. In selection test of resistant shiitake strains, 67 out of 77 strains tested were proved to be resistant to D. stigma. Among them, 13 strains including KFRI 5 were effective to inhibit the access of D. stigma, and 7 strains including KFRI 180 remarkably invaded the territory of D. stigma. Among 31 shiitake strains made by hybridization of resistant strains for D. stigma, 8 strains including KFRI 537 inhibited the access of D. stigma, and 4 strains including KFRI 545 invaded the territory of D. stigma. The effects of temperatures and inoculation orders to the resistance were confirmed in PDA plates and test tubes filled with sawdust of Quercus acutissima. Four kinds of temperature treatments as follows were tested: ① continuous incubation at 14℃, ② continuous incubation at 25℃, ③ changing of incubation temperature from 14℃ to 25 ℃ as soon as mycelia of both shiitake and D. stigma meet together, ④ changing of incubation temperature from 25℃ to 14 ℃ as soon as mycelia of both shiitake and D. stigma meet together. Three kinds of inoculation procedure were tested: ① inoculation of shiitake 3 days ahead of D. stigma inoculation, ② inoculation of D. stigma 3 days ahead of shiitake inoculation, ③ simultaneous inoculation of both fungi. In PDA plate test, the strain KFRI 137 showed outstanding ability to inhibit mycelial growth of D. stigma and the strain KFRI 180 invaded into the territory of D. stigma in most of treatments. Hybrid strains, KFRI 545, 546, and 547 were more resistant than their parent strains, KFRI 488 and 405. In test tube examinations, all the strains of shiitake showed high resistance at the treatment of change in temperature from 14℃ to 25℃ when mycelia of both shiitake and D. stigma meet together. On the other hand, resistance of all the strains growing at 25℃ decreased when the temperature was changed into 14℃ after mycelia of both fungi. In these cases, the resistance reached to 7∼20% of the highest resistance. The strain KFRI 259 invaded the territory of D. stigma, contrary to PDA plate test. Among the strains, KFRI 393 strain was the most resistant under the continuous incubation at 25℃. 표고골목 해균인 주홍꼬리버섯을 방제하기 위해 저항성 표고균주 선발을 시도하였다. 저항성 표고균주 선발 시험에서, 사용된 77개 균주 중 67개 균주가 저항력을 가지는 것으로 확인되었다. 이들 67개 균주 중 KFRI 5를 포함한 13개 균주가 주홍꼬리버섯균의 접근을 막는데 가장 큰 영향을 미쳤고 KFRI 180을 포함한 7개 균주가 주홍꼬리버섯의 영역으로 가장 많이 침범했다. 교잡을 통해 만든 31개 균주 중 KFRI 537을 포함한 8개 균주가 주홍꼬리버섯균의 접근을 막는데 가장 큰 영향을 미쳤고 KFRI 545를 포함한 4개 균주가 주홍꼬리버섯의 영역으로 가장 많이 침범했다. 온도와 접종순서의 변화에 따른 각 균주의 저항력 차이를 확인하기 위해 PDA 평판배지와 상수리나무톱밥을 넣은 시험관배지를 사용했다. PDA 평판배지 시험결과, 대부분의 처리구에서 KFRI 137은 주홍꼬리버섯에 대해 뛰어난 접근 억제력을 보였고 KFRI 180은 뛰어난 침범력을 보였다. 교잡균인 KFRI 545, 546, 547은 모균주들인 KFRI 488과 405에 비해 높은 저항력을 보였다. 시험관배지 시험결과, 표고균과 주홍꼬리버섯균이 대치를 하는 시점부터 25℃에 두었을 때 모든 균주가 높은 저항력을 나타냈다. KFRI 393은 25℃에서 지속 배양할 때 다른 균주에 비해 높은 저항력을 나타냈다. 또한 모든 균주들에서, 25℃ 배양 후 두 균이 대치를 할 시기에 14℃로 옮겨 배양한 처리구는 다른 처리구들에 비해 낮은 저항력을 보였다. 이 때의 저항력 정도는 각 균주 최고치의 7-20%에 불과했다. 또한 KFRI 259는 PDA 평판배지에서와 달리, 주홍꼬리버섯의 영역을 침범했다.

표고 톱밥재배시 중온성 품종의 종균 형태에 따른 생산성 비교

이봉훈 외 한국버섯학회 2008 한국버섯학회지 Vol.6 No.1

종균의 종류에 따라 중온성 품종의 생산성이 어떻게 달라지는지를 확인하고자 연구를 수행하였다. 버섯의 발생 경향은 세 처리구 모두 비슷했으며, 성형종균과 액체종균 처리구는 2차발생까지 총수확량의 50% 이상, 4차까지 80% 이상 발생되었다. 기형버섯조사에서는, 세 처리구 모두 2차 발생까지만 기형버섯이 발생되었다. 하지만 기형버섯 발생률에 있어서는 다른 처리구들에 비해 액체종균 접종구에서의 발생률이 낮았다. 수확량조사에서는 액체종균을 접종했을 때 배지당 411 g으로 처리구들 중 가장 많았고 10 g이 넘는 버섯도 309 g으로 톱밥종균과 성형종균에 비해 많았다. 그리고 발생된 자실체 개수는 톱밥종균과 성형종균 간에 유의성이 있었지만 자실체 개당 무게는 세 처리구 간에 유의성이 없었다. Studies were processed to confirm the difference of the shiitake productivity according to different spawn shapes(sawdust, plug-shaped and liquid spawns) on middle-temperature type strain. A tendency of fruiting was similar among three treatments, and treatments inoculated with plug-shaped spawn and liquid spawn produced over 50 % of total yield until 2nd flushing period and 80 % of total yield until 4th flushing period. In investigation of deformed fruitbodies, all of three treatments occurred until 2nd flushing period. However, in rate of deformed fruit-bodies, treatment inoculated with liquid spawn was lower than others. In investigation of yield, the amount produced on treatment inoculated with liquid spawn was 411 g per medium and it was highest among treatments. And the amount of fruit-bodies over 10 g was higher than others. Also, the number of fruit-bodies between sawdust and plug-shaped spawn was different, but each weight of fruit-bodies among three treatments was not different.

금속 다공성 물질 내부의 유동특성

김봉훈 대구대학교 특수교육재활과학연구소 2010 産業技術硏究 Vol.21 No.1

An experimental study was conducted to investigate the effect of micro structure of metal wick on the permeability governing heat transport capacity of looped heat pipe(LHP). Three different samples, made of powder-sintered nickel and characterized from analysis of SEM images, were prepared by the combination of three different values in both porosity and sample length. Permeability measurements were performed using a steady-state filter test apparatus. For each sample, both pressure drop across the test section and corresponding flow rate were measured and parametric analysis was made to find ominant factors. Test results showed that mass flow rate linearly increased with pressure drop and rate of ncrease also increased with magnitude of porosity. It was found that degree of porosity played an important role in the evaluation of permeability.

CNC LATHE 성능개선을 위한 검사방법에 대한 연구

김봉훈,윤재웅 대구대학교 산업기술연구소 2009 産業技術硏究 Vol.20 No.1

An experimental investigation was conducted to analyze the effect of geometric structure of CNC lathe on the accuracy of machined work pieces. First, a detailed data base for summarizing inspection methods and items was made through literature reviews on the related standards and inspection guide lines. Secondly, performance tests were performed using a test equipment corrected according to the inspection guide lines. Results showed that accuracy of the work pieces was well controlled and satisfied geometric tolerances of the inspection guide lines proposed in this paper.

뉴톤-랍슨법에 의한 전력조류계산 프로그램 개발

李鳳容,金正勳,沈建輔 弘益大學校 1981 弘大論叢 Vol.13 No.-

A power flow solution program was developed based on the Newton-Raphson iterative technique. Although this technique has been widely adopted overseas, little attention has been given to it domestically. Memory requirement and matrix inversion are the main short-comings of this technique. In this paper, one dimensional non-zero element storage scheme was developed and achieved a great reduction in matrix memory. Further, matrix inversion was efficiently performed by using Choleski's decomposition technique. The results ar demonstrated with 5 and 11 Bus Systems. 제1장 서 론 제2장 본 론 1. 모선어드미턴스 구성 앨고리즘 2. 삼각함수의 근사화 계산 3. 뉴톤-랍슨 전력 조류계산의 개요 4. 자코비안 구성 앨고리즘 5. 촐스키의 분해법 6. 자코비안의 분해 및 수정 7. 선로의 유효 및 무효전력계산 8. 전력조류계산의 플로우챠트 제3장 사례연구 제4장 결 론 A power flow solution program was developed based on the Newton-Raphson iterative technique. Although this technique has been widely adopted overseas, little attention has been given to it domestically. Memory requirement and matrix inversion are the main short-comings of this technique. In this paper, one dimensional non-zero element storage scheme was developed and achieved a great reduction in matrix memory. Further, matrix inversion was efficiently performed by using Choleski's decomposition technique. The results ar demonstrated with 5 and 11 Bus Systems.

반응성 가열 증착법에 의한 수소화된 비정질 게르마늄 제작

梁承勳,李原鎭,朴奉斗 慶北大學校 1984 論文集 Vol.37 No.-

The addition of hydrogen during the evaporation of germanium has been shown to improve the electrical properties of the resulting a-Ge film considerably by saturation of dangling bonds, if the dissociation of molecular hydrogen by high voltage AC discharge takes place. Evaporation onto a substrate at 200℃ in the mood of hydrogen plasma has been shown to yield a film which shows a high resistance and an activated type conduction(with an activation energy of 0.38eV) above room temperature.

고장모드 분석 프로그램을 통한 공작기계의 신뢰성 평가

김봉석,이수훈,송준엽,이승우 한국공작기계학회 2005 한국생산제조학회지 Vol.14 No.1

For reliability assessment for machine tools, failure mode analyses by two viewpoints were studied in this paper. First, this study developed the reliability data analysis program, which searches for optimal failure distribution like failure rate or MTBF(Mean Time Between Failure) using failure data and reliability test data of mechanical parts in the web. Moreover, this data analysis program saves both failure data or reliability test data and their failure rate or MTBF for database establishment. Second, this paper conducted failure mode analysis through such performance tests as circular movement test and vibration testing for machine tools when reliability data is not available. A developed web-based analysis program shows correlations between failure mode and performance test result, and also accumulates all the data. These kinds of data analysis programs and stored data furnish valuable information for improving the reliability of mechanical system.

목조건축의 도리결구재의 유형 분류 : 16세기 이전의 건축물을 중심으로 Focused on structures built before the 16th century

주상훈,전봉희 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(계획계)

The purpose of this study is to classify a purlin support type and its materials of Korean wooden structures and Chinese ones and consider it with the times and regions. There are side connecting bace(Takgak), slanting strut(Chasu), connecting beam(Chagyun), curved connecting beam(Charak), purlin-support(Dori-anchogong) and so on besides a basic beam in the type of purlin support forms have several characteristics in accordance with the times and regions. According to this classification. Structures of Koryo have a connection with structures of Song or Won dynasty in China. Periodic and regional characteristics of Korean wooden structure can be found in the use of curved Chasu or decorated Dori-anchogong.

매크로/마이크로 레벨 볼트 결합 구조물에서의 실험적/해석적 방법을 통한 시스템 특성 비교

김봉석,이성민,이문구,이수훈,Jun Ni,송준엽,이창우,하태호 한국공작기계학회 2006 한국공작기계학회 추계학술대회논문집 Vol.2006 No.-

As the micro technology and miniaturization of a structure and component in the last couple of decades have been concretely realized, the understanding of structural and dynamic characteristics and prediction of dynamic behavior are indispensable to the miniaturized structures and micro machines. This paper shows the effect of dynamic characteristics in bolt-jointed structures by comparing natural frequencies and mode shapes between macro and micro-scale beams through experimental modal analysis and finite element analysis with a1 8 test models by materials, by size, and by joining condition; that is, SS41 and A16061-T651, micro and macro, and monolithic beam and bolt-jointed beam.