A novel role for bone-derived cells in ankylosing spondylitis: Focus on IL-23

Jo, Sungsin,Koo, Bon San,Lee, Bitnara,Kwon, Eunji,Lee, Young Lim,Chung, Heekyoung,Sung, Il-Hoon,Park, Ye-Soo,Kim, Tae-Hwan Elsevier 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>The main aim of this study are to explore the role of bone-derived cells (BdCs) in ankylosing spondylitis (AS) and determine the underlying molecular mechanisms of IL-23 production. Primary BdCs were isolated from diced bone of facet joints obtained during surgery from seven AS patients and seven disease control (Ct) patients. Osteoblastic activity of BdCs was assessed by measuring their alkaline phosphatase activity and by alizarin red staining. Osteoblast and endoplasmic reticulum (ER) stress-related genes were assessed by quantitative PCR, immunoblotting, immunofluorescence, and immunohistochemistry. In addition, expression of IL-23 in response to BIX (selective BIP inducer X)-induced ER stress was evaluated by qPCR and ELISA. Protein interaction and binding to IL-23 promoter were confirmed by Immunoprecipitation and Chromatin immunoprecipitation, respectively. Transcript levels of genes involved in osteoblast function, as well as of the ER stress marker were higher in the AS group than the Ct group, and elevated RUNX2, BiP and IL-23 expression were observed in the BdCs, serum, and bone biopsies from the AS group. BIX-induced ER stress stimulated osteoblastic activity and IL-23 secretion by upregulating RUNX2 expression. Furthermore, in AS BdCs, RUNX2 interacted with C/EBPβ to bind to IL-23 promoter and RUNX2 knockdown suppressed IL-23 secretion. These finding may provide a molecular mechanism involved in sustained ER stress in AS BdCs stimulates the activation of RUNX2 and C/EBPβ genes, leading to IL-23 production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bones and its-derived cells from patients with AS showed an increase in ER stress. </LI> <LI> IL-23 cytokine was significantly higher in AS patients than in healthy controls. </LI> <LI> Inducing ER stress in AS exhibited an increase of bone-related genes. </LI> <LI> Inducing ER stress in AS was accompanied with augmentation of IL-23 cytokine. </LI> <LI> ER stress-induced RUNX2 is involved in IL-23 secretion and bone-related genes. </LI> </UL> </P>

Characterization of effector memory CD8+ T cells in the synovial fluid of rheumatoid arthritis.

Cho, Bon-A,Sim, Ji Hyun,Park, Ji Ah,Kim, Hye Won,Yoo, Wan-Hee,Lee, Seung-Hyun,Lee, Dong-Sup,Kang, Jae Seung,Hwang, Young-Il,Lee, Wang Jae,Kang, Insoo,Lee, Eun Bong,Kim, Hang-Rae Springer 2012 Journal of clinical immunology Vol.32 No.4

<P>Little is known about the cellular characteristics of CD8(+) T cells in rheumatoid arthritis (RA). We addressed this by investigating whether the frequency of the CD8(+) T cell subsets and their phenotypic characteristics are altered in the peripheral blood and synovial fluid (SF) from patients with RA. In this study, CD8(+) T cells, mainly CD45RA(-) effector memory (EM) CD8(+) T cells, were increased significantly in the SF, but not in the peripheral blood from RA patients, compared with healthy controls. The synovial EM CD8(+) T cells were activated phenotypes with high levels of CD80, CD86, and PD-1, and had a proliferating signature in vivo upon Ki-67 staining, whereas the Fas-positive cells were prone to apoptosis. In addition, EM CD8(+) T cells in the SF were less cytotoxic, as they expressed less perforin and granzyme B. In particular, the proportions of synovial fluid mononuclear cells that were CCR4(+)CD8(+) T cells and IL-4-producing CD8(+) T cells (i.e., Tc2 cells) were significantly higher than those in peripheral blood mononuclear cells of patients with RA and healthy controls. In addition, the number of IL-10-producing CD8(+) suppressor T (Ts) cells increased significantly in the SF of RA patients. Especially, CD8(+) T cells were inversely correlated with disease activity. These findings strongly suggest that EM CD8(+) T cells in the SF are increased, likely because of inflammation, and they may be involved in modulating inflammation, thereby affecting the development and progression of RA.</P>

신선기보탕(神仙氣步湯)이 Collagen Ⅱ 유발관절염(誘發關節炎)의 발생 및 치료에 미치는 영향

구본길 ( Bon Kil Koo ),신혜란 ( Hye Lan Shin ),진광선 ( Kwang Seon Jin ),윤일지 ( Il Ji Yoon ),오민석 ( Min Suck Oh ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.4

Objectives: Cytokines play key roles in spontaneous autoimmune arthritis in CIA(collagen induced arthritis) mice, a model of rheumatoid arthritis. The object of study is to evaluate the effect of IL-6, TNF-α on development of collagen induced arthritis, on humoral and cellular immunity and on the endogenous production of IL-6, TNF-α in DBA/1J mice. Methods: DBA/1J mice were immunized with 100㎍ bovine type II collagen in 0.1㎖ Freund`s complete adjuvant. There are three groups in this study, Which are wild type(WT), CIA-control and CIA-SSKBT(350㎎/㎏). Levels of anti-collagen antibodies, serum IL-6 and TNF-α were measured at the end of study. At week 6, CIA-control group developed arthritis while CIA-SSKBT group showed far less incidence and severity of arthritis. Result and Conclusions: This study shows that SSKBT inhibits inflammatory effect of IL-6, TNF-α.

이자율과 주가 변동성

구본일 연세대학교 경영연구소 1998 연세경영연구 Vol.35 No.2

주가 수익률 변동성의 정확한 추정은 자산가격결정모형들의 정확한 검증이나 파생금융상품 가격결정의 정확성 등의 분야에 필수 불가결한 요소이다. 이 변동성의 추정에 있어서 거래량을 설명변수로 도입하여 그 정확성을 높이려는 연구는 활발히 진행되었다. 그러나, 거래량에 비해서 주가 수익률의 결정에 더 분명한 관련이 있는 이자율을 설명변수로 채택한 연구는 의외로 찾아보기 힘들다. 본 연구는 국내에서 아직 활발하게 전개되지 않은 이 분야의 실증분석을 수행하였다. 주간 주가 및 이자율 데이터를 사용한 검증 결과, 전기의 기대하지 않았던 금리수준의 상승이 현재의 주가 수익률의 변동성에 유의적인 영향력을 가지는 것으로 나타났다. 그러나, 검증모형에 따라 결과의 일관성이 부족하여 더 확대된 데이터를 이용한 추가적인 실증분석의 필요성이 제기되었다.

Pulmonary Echinococcosis - A Case Report -

고일향,이진,구본일,손광현 인제대학교 1986 仁濟醫學 Vol.7 No.1

Pulmonary Echinococcosis (폐포충증)은 아직까지는 우리나라에서 보기드문 질환인 바 근래 국제 교류가 빈번해지면서부터 문제가 되기 시작하고 있으며, 특히 중동 지역에서 근무한 병력이 있는 환자에서 발생한 국내 보고가 수 예 있을 뿐이다. 저자들의 수술 치험 1례를 보고하는 바이다. Echinococcosis (hydatid disease) is endemic to the Mediterranean region, South America, Australia, and New Zealand where livestock, especially, sheep, are kept. The disease is considered to be rare in this country. However, recently, with increasing numbers of Koreans visiting those countries where hydatid disease is endemic especially Mid East region, the reports of hydatidosis are increasing, in the liver, but very rarelly in the lung. Authors experienced a case of pulmonary hydatid disease in the 30 year old male which was removed surgically on July 1985. We report the case to emphasize the need to consider this condition adding to other differential diagnosis of the mass lesion in the lung in our country and the literatures reviewed.

餘暇社會化 參與에 寄與하는 要因體系

千英一,具本行 중앙대학교 스포츠과학연구소 2000 體育硏究 Vol.- No.13

Ther are childhood and the period of adult on leisure socialization participation, and various factors such a family, school, attitude have an influence on it. The purpose of this research is to establish education of leisure socialization participation and valuable leisure outlook by organizing these factors. 1. Leisure socialization participation is divided into life habit, basic knowledge, attitude, value, behavioral psychology of childhood and the period of adult. 2. Leisure activity is experienced through childhood, adolescence, and a period of adult; however, leisure behavior in childhood is not a decisive factor of leisure behavior in the period of adult. 3. Family and school are the best superintendents, and they have an interactive effect. Many following researches are required. 4. Factors of perceived leisure aptitude reflect on differences quantitatively and qualitatively according to development stage, and these changes are represented to perceived ability limits of individuals. 5. When attitude to leisure is measured at the same level of intention, it can be a good predictive material about the intention of leisure activity participation.

도시유역에서의 유출해석을 위한 시험유역의 개발

서규우,송일준,구본석,김남길 동의대학교 산업기술개발연구소 2001 産業技術硏究誌 Vol.15 No.-

The purpose of this study is development of experiment catchment for hydrological monitoring at Urban Watershed. The location of test basin is Dong-Eui University, Gaya Dong, Pusan, Karea. For the collecting of basic hydrologic data of experiment catchment, topography inquiry and survey was conducted, and sub-basins data, such as areas of test basin and impervious layer, were arranged. On the basis of that result, a drainage pattern was drawn up and for the collection of hydrological data such as the rainfall data and the water-level data-EMS installed at Dong-Eui Univ. in 1998, and AWS-DEU(Automatic Water-level Station-Dong-Eui Univ) installed at the point of the end discharge.

Power analysis attack 방어대책에 대한 연구

이훈재,최희봉,박일환 경운대학교 산업기술연구소 2001 産業技術硏究論文誌 Vol.3 No.2(B)

Attacks have been proposed that use side information as timing measurements, power consumption, electromagnetic emissions and faulty hardware. Elimination side-channel information or prevention it from being used to attack a secure system is an active ares of research. In this paper, differential power analysis techniques used to attack DES are reviewed and analyzed. Finally, we propose the prevention of DPA attack.

후복막강내 발생한 Castleman병 1례

남성진,이동욱,박찬규,정시욱,김추성,조성래,구본천,양사규,이일웅,박정자 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

Castleman' s disease is a rare, reactive lymphoid proliferation of unknown cause and pathogenesis. Depending on histololgic findings, it has been divided into two distinct histologic subtypes that include hyaline-vascular type(90%) and plasmacell type(10%). This disease occurs most commonly in mediastinal lymph nodes and cervical lymph nodes, inguinal lymph nodes respectively. But occurs rarely in retroperitoneum. We report a case of hyaline-vascular type asymptomatic Castleman' s disease of the retroperitoneum in a 47-year-old man.

The variation of growth and heading date in cultivating early maturing variety, Unkwangbyeo, in Honam plain area

Bon Il Ku,Hong Kyu Park,Weon Young Choi,Nam Hyun Back,Min Gyu Choi,Sang Su Kim,Chung Kon Kim 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.39 No.-