국내 주요 호수의 육수학적 조사(4): 주암호

김범철,박주현,허우명,임병진,황길순,최광순,최종수 江原大學校 附設 環境硏究所 2001 環境硏究 Vol.18 No.-

In this study limnological characteristics of Lake Juam was surveyed from June 1993 to May 1994 in order to provides important information regarding water resources. Seschi disc transparency, epilimnetic chlorophyll a(chl-a), tatal nitrogen(TN), total phosphorus(TP) concentration and primary productivity were in the range of 2.0~4.5 m, 0.9~13.6 mgChl/㎥, 0.78~2.32N/l, 11~56 mgP/㎥, 270~2,160mgCm^(-2)day^(-1), respectively. On the basis of TP, Chl-a and Secchi disc depth, the trophic state of Lake Juam can be classified as mesotrophic lake. The phosphorus inputs from non-point sources are concentrated in heavy rain episodes during the monsoon season. As a result, phosphorus concentration are higher in summer than in winter. TP loading from the watershed were estimated to be 0.9 gPm^(-2)yr(-1), which correspond to a boundary of the critical loading (1.0 gPm^(-2)yr(-1), which correspond to a boundary of the critical loading (1.0 gPm^(-2)yr(-1)) for eutrophication. From the results of the algal assay, both phosphous and nitrogen act as limiting nutrients in algal growth. The seasonal succession of phytoplankton community structure in Lake Juam was similar to that observed in other temperate lakes. Diatoms(Asterionella formosa and Aulacoseira granulata var. angustissima) were dominant in spring and winter, cyanobacteria (Microcystis aeruginosa, M. sp. and M. viridis) were dominant in warm season. The organic carbon, nitrogen and phosphorus content of lake sediment were 9.5~14.0 mgC/g, 1.01~1.82 mgN/g, and 0.51~0.65 mgP/g, respectively. The allochthonous organic carbon loading from the watershed and autochthonous organic carbon loading by primary production of phytoplankton were determined to be 1,122 tC/yr and 6,718 tC/yr, respectively. To prevent eutrophication of Lake Juam, nutrient management of watershed should be focus on reduction of fertilizer application, proper treatment of manure, and conservation of topsoil as well as point source.

STEP 표준을 이용한 열교환기 형상 데이터 모델링

오해진,이범석 경희대학교 산학협력기술연구원 1996 산학협력기술연구논문집 Vol.2 No.-

Sharing and exchanging of product data are critical to the successful business in the industries. Engineers in the process industries use numerous software packages, some commercial and some private. Process and equipment designs are passed from operating companies to engineering and construction companies on paper. In turn, E&C companies will pass paper specifications to fabrications and contractors. The data must be produced to be accurate, up-to date, etc. A plant database must be kept even if the plant is discommissioned. These needs to be a single copy of each data thorough the life-cycle of the plant. The ISO-STEP developments for the actual implementation of data exchange programs and interfaces are briefly mentioned in this work. Especially, the shape of heat exchangers which are most widely used in the chemical plant is considered to be modeled based on ISO-STEP standard.

국내 주요호수의 육수학적 조사 (1) : 옥정호

김범철,박주현,이병진,허우명,황길순,최광순,채기숙 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

The limnological survey of Lake Okjong was conducted for one year from June 1993 to May 1994 on the monthly basis. The loading of phosphorus, nitrogen and organic carbon from the watershed into the lake were monitored at the main in flowing sites. Secchi disc transparency , epilimnetic chlorophyll a, total nitrogen, total phosphorus concentration and primary production were in the range of 1.3~4.H m, 2.4~ 18.7 mg Chl/m³. 1.25~2.87 mg N/l, 7~65 mg P/m³, 325~2,113 mg C/m²/day, respectively. TN/Tl atomic ratio varled from 129 to 443. N/P ratio decreased in summer because phosphorus concentration was higher than in winter, while nitrogen did not vary much. The seasonal succession of phytoplankton was distinct. In winter and spring, diatoms, Aulacoseira granulata var. angustissima and Aulacoseira italica were dominant while cyanobacteria, Microcystis sp.,M. ichthyovlabe, Phormidium sp. and P.valderianum var. tenuis were dominant in warm seasons. The dominant zooplankton species were Thertmocyclops taihokuensis in warm seasons while Boosmina longirostris were dominant in cold seasons. The organic carbon, nirtogen and phosphorus content of lake sediment were 8.0~14.8, 0.59~0.71, 1.14~1.87 ng N/g, respectively. The sediment of Lake Okjong can be classified as oligohumic based on C/N ratio. The total phosphorus loading from the watershed and fishfarm were estimated to be 2.7g P/m²/yr, which far exceeded the critical loading for eutrophication. The organic carbon loading from the watershed and primary production were determined to be 998t C/yr, 6,348t C/yr, respectively. Most of organic carbon was contributed by autochthonous primary production of phytoplankton. Trophic state of Lake Okjong can be classified as eutrophic

pHOxsFV벡터와 배아주간세포를 이용한 형질전환생쥐 생산 기초연구

이훈택,이봄이,정길생,김진회 건국대학교 동물자원연구센터 1998 動物資源硏究誌 Vol.19 No.-

pHook™-1 hapten 4-ethoxy-methylene-2-phenyl-2-oxazolin-5one(phOX)의 단일 항체 sFV를 암호화 하고 있으며, murine의 Ig k-chain V-J2-C 영역유래의 signal peptide에 의하여 항체를 세포 표면에 배열시키도록 고안되어 있다. 또한, 항체를 세포막 바깥쪽에 부착되어 있도록 하기 위해 PDGFR 유래의 transembrane domain의 C 말단에 결합되어 있다. 이렇게 고안된 vector을 발현하는 세포는 세포막에 sFV을 발현함으로, phOX로 코팅된 자석베드를 이용하여 배양체로부터 목적의 유전자를 발현하는 세포만을 분리할 수 있을 것이다. 따라서, 본 연구는 pHook™-1 유전자를 co-transfection함으로써 목적의 유전자를 가진 배아주간세포를 단시간 내에 효율적으로 선발하기 위하여 실시하였다. 또한, 배아주간세포에서 목적의 DNA 발현 또는 존재를 검증하기 위해 DNA 발현 또는 존재를 검증하기 위해 PCR 방법과 조직화학적 방법을 사용하였다. 형질전환유전자 발현을 transfection(유전자 전이) 후 4∼14일 사이에 모든 배아주간세포에서 확인되었다. Magnetic bead를 이용하여 선발된 세포에서 co-transfected DNA는 배아주간세포에서 효율절으로 삽입되었으며, 선발된 세포의 약 90%는 co-transfected 유전자를 발현하였다. 이 결과는 세포생리학에서 특이 유전자의 급성변이와 만성변이를 연구하거나, 또는 형질전환동물을 생산하기 위해 pHook™-1 목적유전자와 함께 전이함으로서 효율적으로 목적의 유전자를 가진 세포를 선발 가능함으로써 보다 간편하게 형질전환도 동물의 생산에 이용 가능하다는 사실을 확인하였다. pHook™-1 encodes a single-chain antibody(sFv) directed toward the hapten 4-ethoxy-methylene-2-phenyl-2-oxazolin-5-one(phOx): the signal peptide from the murine Igk-chain V-J2-C region is fused in front of coding region of the sFv to direct the antibody to the plasma membrane. The antibody is fused at the C-termius to the transmembrane domain from the platelet derived growth factor receptor(PDGFR), allowing the antibody to be anchored and displayed on the extracellular side of theemmbrane. Transfected cells expression sFv can be isolated from whole cultures by using magnetic coated with phOx and a strong magnetic strand. Thus, the present study was designed to apply the embryonic stem cells by using pHook™-1 . Cell-transduction efficiency was measured by morphometric analysis. Polymerase chain reaction and histochemistry were used to detect the presence and/or expression of objective DNA in embryonic stem cells. Transgene expression was detected in all cases between 4 and 14 day after transfection. In selected cells using magnetic bead, co-transfected DNA was also incorporated efficiently in embryonic stem cells and approximately 90% of the selected cells expressed co-transfected gene. This result suggested that this selection system can be used as a feasible tool, when pHook™-1 is cotransfected with objective gene, to isolate and study for acute and chronic changes of a specific gene in cellular physiology.

ANATOMICAL EXPANDER와 IMPLANT를 이용한 유방 재건의 경험

고법민,박원진,김재중,하범준,이재승,신명수 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

The use of a tissue expander and implant is the simplest option for breast reconstruction after mastectomy. Use of a round dome-shaped prosthesis and the commonly used one-stage technique with a Becker type prosthesis, however, often produces an undesirable upper pole fullness. To overcome this and to achieve improved aesthetic results, we started using an anatomically-shaped expander and implant, as described by Maxwell, as a two-stage breast reconstruction. We reviewed the results of our 21 reconstructed breasts in 22 patients who were deemed suitable for reconstruction using this technique since January 1995. The most commonly used expander was 350cc (range 350-550cc) and an average of 4.2 inflations were required before replacing the expander with a permanent implant. All the expanders were placed in submuscular pockets and implant volume. The longest follow-up was 36 months. Few complications developed and most patients were satisfied with the results. We found that the anatomically-shaped expander and implant produced better aesthetic results compared to a done-shaped prosthesis.

Suwa호 방류수내 남조류 독소(microcystin)의 일변화

김호섭,김범철,박호동,片上幸美,황순진 江原大學校 附設 環境硏究所 2001 環境硏究 Vol.18 No.-

The temporal and diel changes of cyanobacterial cell density, species composition, and cyanobacterial toxins (microcystin-RR, -YR, -LR) were examined for the outflow water of Lake Suwa in Japan from May to October, 1998. The highest total cell densities of Microcystis were observed in July and September, when the dominant phytoplankton was Microcystis ichthyoblabe and M. viridis, respectively. Both the species composition and total cell density of Microcystis affected the variation of the concentration of three microcystin variants. Only microcystin-RR(MC-RR) and -LR(MC-LR) were detected in July when Microcystis ichthyoblabe dominated, while microcystin-RR, -YR(MC-YR) and -LR were detected in August and October when Microcystis viridis dominated. The microcystin concentration and the cell density of Microcystis in the outflow water showed diel variations; the ratio of maximum to minimum value was 3~20 fold for microcystin concentration, and 5~31 fold for cell density. The diel variations of toxin concentration as well as Microcystis cell density was closely related to the diel variation of wind. During the windy period, when higher speeds occurred in the afternoon hours than morning hours, both the cell density of Microcystis and microcystin concentration tended to increase in the morning and decrease in the afternoon. The results of this study suggest that controlling the timing of lake discharge at the floodgate or intake tower can be useful for water resource management with respect to decreasing cyanobacteria biomass within intake water.

합천군 치면열구전색사업의 영구치 우식증 예방효과

김선창,이선미,김지영,김진범 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.3

A clinic-and school-based fissure sealant program was initiated by the community health center in Habchon, Korea in 1996. Fissure sealants were applied limitedly to first molars of all 6-year olds, grade one of elementary schools in 1996. The program was expanded to more children annually by one grade. Children for sealants included all 6-10-year olds in grades one through five in 2000. The application of sealants was expanded to premolars besides molars. Children who lives close to health center or health sub-center visit the dental clinic of health center or sub-center by themselves for sealants. However, dentists and oral hygienists of health center or sub-center visits remote elementary schools and provide sealants at schools. To evaluate the effectiveness of a clinic-and school-based fissure sealant program in Habchon-gun, dental examinations were conducted on 6-14-year old 1,957 children of elementary and middle schools in Habchon-gun and control group, 1,277 children in 2000. The surveyed data were analyzed with SPSS statistical package. The obtained results were as follows; 1. DMF rates were 7.5% at &year olds and 53.5% at 6-and 10-year olds benefited by the sealant program of Habchon-gun Health Center, DMF rates in sealant group was lower than control group. 2. DMFT scores were 0.11 at 6-year olds, 1.33 at 10-year olds benefited by the sealant program of Habchon-gun Health Center. DMFT scores in sealant group was lower than control group. 3. The caries reduction rate from the sealant program was calculated as 56.0%, 31.4%, 43.1%, 28.9%, 30.4% at 6-,7-, 8-, 9- and 10-year olds respectively by the difference of DMFT scores between sealant group and control group. 4. Percentage of children with sealants was 52.8% and 95.5% at 6-and 10-year olds respectively. Mean number of teeth with sealants was 0.83 and 5.06 at 6-and 10-year olds respectively. 5. The fissure sealant program of Habchon-gun Health Center from 1336 to 2000 was evaluated to be effective to reduce dental caries on permanent teeth. These results substantiate that a significant reduction in dental caries can be achieved by the fissure sealant program.

한국 초·중·고등학생들의 측두하악장애 유병상태와 악안면외상과의 관련성

정기호,권호근,김진범,최충호 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.2

The purpose of this population-based epidemiologic study was to clarify the tempromandibular disorders prevalence, the maxillofacial trauma experience rate and the relation between maxillofacial truma and tempromandibular discorders in 6-14 and 16 years old korean children who were selected through the stratified random sampling method. the results are as follows; 1. The prevalence of tempromandibular clicking sounds was 1.28% in elementary students, 11.05% in middle school students, 20.07% in high school students, with an overall 6.30%. The result of the statistical test showed that girls experienced more tempromandibular clicking sounds than boys(p<0.05). 2. The prevalence of tempromandibular pain was 0.51% in elementary students, 1.95% in middle school students, 7.04% in high school students, with an overall 20.07%. 3. The prevalence in mouth opening limitation was 0.04% in elementary students, 1.36% in middle school students, 5.85% in high school students, with an overall 1.06%. 4. The percentage of treatment experience for the tempromandibular disorders was 0.00% in elementary students, 0.77% in middle school students, 2.12% in high school students, with an overall 0.46% 5. The maxillofacial trauma experience rate was 5.45% in elementary students, 9.11% in middle school students, and 11.89% in high school students, with an overall 7.22%. Males experienced more maxillofacial trauma than females(p<0.01), and urban students experienced more than rural students)p<0.05). 6. The results of the relation betwwen maxillofacial trauma and tempromandibuar disorders showed there were statistically significant realtionship between the tempromandibular experience and the clicking sound(odds ratio 1.86), and between the maxillofacial trauma and tempromandibular disorders(odds ratio 1.79).

족저압력분포 측정장비를 이용한 골프 스윙시 족저압 분석

이동기,이중숙,이범진,이훈식,김용재,박승범,주종필 한국운동역학회 2005 한국운동역학회지 Vol.15 No.1

D. K. LEE, J. S. LEE, B. J. LEE, H. S. LEE, Y. J. KIM, S. B. PARK, J. P. JOO. PIantar foot pressure analysis during golf swing motion using plantar foot pressure measurement system. Korean Journal of Sport Biomechanics, Vol. 15, No. 1, pp. 75-89, 2005. In this study, weight carrying pattern analysis and comparison method of four foot region were suggested. We used three types of club(driver, iron7, pitching wedge). This analysis method can compare between top class golfer and beginner. And the comparison data can be used to correct the swing pose of trainee. If motion analysis system, which can measure the swing speed and instantaneous acceleration at the point of hitting a ball, is combined with this plantar foot force analysis method, new design development of golf shoes to increase comfort and ball flight distance will be available. 1. Address acting, forces concentrated in rare foot regions and lateral foot of right foot. Back swing top acting, relatively high force occurred in medial forefoot region of left foot and forefoot region of right foot. Impact acting, high force value observed in the lateral rarefoot region of left foot and medial forefoot region of right foot. Finish acting, force concentration observed on the lateral region and rarefoot region of left foot. 2. Forces were increased in address of right foot with clubs length increased. All clubs, back swing top acting, high force value observed in the lateral forefoot region of right foot. All clubs, in impact, high force value observed in the lateral rarefoot region of left foot and medial forefoot region of right foot. Finish acting, force concentration observed on the rarefoot region in driver and lateral foot region in iron on left foot. 3. Right foot forces distribution were increased in address, back swing top and left foot force distribution were increased in impact, finnish

도시 저소득층 고령 주민의 구강건강실태

허복,박수병,김진범,손우성 韓國保健敎育學會 1996 보건교육건강증진학회지 Vol.13 No.1

The oral health status and practices related to oral health among 44-54-year-, 564-year- and 65-year-old needy residents were assessed. The subjects were the residents at the Unbong Permanent Rental Apartment, Bansong-dong, Haeundae-gu, Pusan, Korea. An oral epidemiological survey was undertaken to determine the status of dental caries and periodontal health. Periodontal health were analyzed by the tool of CPITN(Community Periodontal Index of Treatment Needs). A questionnaire was used to interview for the practices of toothbrushing. The numbers of subjects were 192 for the interview on toothbrushing, 228 for the survey of dental status and 208 for the survey of periodontal status. The major results were as follows: 1. Toothbrushing frequencies per day were 1.9 among 44-54-year-, 1.7 among 55-64-year- and 1.7 among 65+-year-subjects. Percentages of after-meal-toothbrushings among total brushing frequencies per day were 73.7% among 44-54-year-, 70.6% among 55-64-year- and 76.5% among 65+-year-subjects. 2. DMFT indices were 15.5 among 44-54-year-, 16.4 among 55-64-year- and 26.6 among 65-year-subjects. Decayed teeth component of DMF teeth were 23.9% among 44-54-year-, 11.6% among 55-64-year- and 62.8% among 65+-year-subjects. Missed teeth component of DMF teeth were 55.59% among 44-54-year-, 62.8% among 55-64-year- and 77.4% among 65+-year-subjects. Filled teeth component DMF teeth were 20.0% among 44-54-year-, 25.0% among 55-64-year- and 10.9% among 65+-year-subjects. 3. Sound permanent teeth were 16.4 among 44-54-year-, 15.6 among 55-64-year-, and 5.4 among 65+-year-subjects. Present permanent teeth were 23.7 among 44-54-year-, 21.6 among 55-65-year- and 10.9 among 65-year-subjects. 4. Subjects who needed professional oral prophylaxis were 75.0% among 44-54-year-, 83.3% among 55-64-year- and 76.9% among 65-year-subjects. Subjects who needed complex periodontal treatments were 16.7% among 44-54-year-, 13.3% among 55-64-year- and 15.4% among 65+-year-subjects. 5. Sextants which needed professional oral prophylaxis were 59.3% among 44-54-year-, 71.5% among 55-64-year- and 71.5% among 65+-year-subjects. Sextants which needed complex periodontal treatments were 5.6% among 44-54-year-, 4.1% among 55-64-year- and 5.7% among 65+-year-subjects. 6. Systematic comprehensive oral health care services should be developed for old needy residents in urban area.