토양처리용 천연물제초제 개발을 위한 생물검정법 개선

김재덕(Jae Deong Kim),황현진(Hyun Jin Hwang),서보람(Bo Ram Seo),최정섭(Jung Sup Choi),김진석(Jin Seog Kim) 韓國雜草學會 2011 Weed&Turfgrass Science Vol.31 No.3

본 연구는 토양처리용 천연물 제초제의 개발을 보다 신속하게 효율적으로 진행하기 위한 방안으로서 실용적인 입장에서 보다 개선된 검정법을 확립하기 위해 수행되었다. 검정하고자 하는 화합물의 량이 50㎎ 이상 충분할 경우, 잡초 4초종(피, 바랭이, 자귀풀, 어저귀)이 파종된 50㎠ 토양 표면적을 가진 폿트에서 온실검정을 함으로서 기존의 방법(토양 표면적이 350㎠인 폿트 이용)에 비해 약제 소요량을 78배 줄일 수 있었다. 이때 검정초기 처리농도는 10,000㎍ mL<;SUP>;-1<;/SUP>; 내외로 하는 것이 적당하였다. 한편 검정용 시료의 량이 10㎎ 이하로서 미량일 경우는 6 well plate를 이용하되 각 well에 밭토양을 담고 4가지 초종을 파종한 다음 실내에서 제초활성을 검정하는 방법이 추천될 수 있었다. 이는 기존의 방법(토양 표면적이 350㎠인 폿트 이용)에 비해 약제 소요량 및 처리용액 부피를 14배 가까이 줄일 수 있었고 실제에 가까운 데이터를 확보할 수 있어 보다 빠른 속도로 천연물 제초제를 개발하는데 도움이 될 것으로 판단되었다. This study was carried out to establish an improved bioassay system on the side of practicality, pre-emergence bioassay which is more effective in developing soil application natural herbicides. A miniaturized method which have a 50 cm² of soil surface area and was efficient by 7 times compared to the existing soil application assay (350 cm² of soil surface area) was established, in which four weed species (Echinochloa crus-galli, Digitaria sanguinalis, Aeschynomene indica, and Abutilon theophrasti) were planted and grown in greenhouse. This would be applicable when the amount of screening compound is much more than 50 mg. The initial application rate was desirable at 10,000 μg mL-1. On the other hand, the 6 well plate assay which has 4 weed species in each well containing upland soil and could be conducted in growth chamber, was established. This assay was resulted in minimizing in level of 1/14 test volume and 1/14 amounts of test compound to the conventional method that has been used for screening of synthetic compounds in KRICT, and applicable for the small amount of test compound (less than 10 mg). Therefore, the improved bioassays established in this study would be helpful for a rapid and efficient development of soil application natural herbicides.

HBsAg 및 류마토이드 인자 공존예의 분석 : Frequency and Clinical Significance

신보문,조종래,백인기,박성섭 대한임상병리학회 1996 대한임상병리학회지 Vol.16 No.5

대장암 환자의 임상양상에 따른 EGFR Mutation의 발생 빈도

오보영,이령아,정순섭,김광호 이화여자대학교 의과학연구소 2013 EMJ (Ewha medical journal) Vol.36 No.1

Objectives: The EGFR plays an important role in tumorigenesis and tumor progression of colorectal cancer, and leads to the activation of intracellular signaling pathways. The use of anti-EGFR-targeted therapy has increased for patients with colorectal cancer, but patients with EGFR mutations will be resistant to anti-EGFR-targeted therapy. The identification of gene mutations is critical in cancer treatment; therefore, the aim of this study is to investigate the incidences of EGFR mutations in colorectal cancer patients in Korea. Methods: We reviewed 58 colorectal cancer patients who underwent operations between 2003 and 2006, retrospectively. We analyzed their EGFR mutations in 4 loci by DNA sequencing. In addition, we analyzed the correlation between the presence of EGFR mutation and patients’ clinicopathologic features. Results: Of the 58 patients, 35 patients were male and 23 were female. Their mean age was 63.28±11.18 years. Two patients (3.45%) were diagnosed as stage Tis, 7 patients (12.07%) had stage I, 24 patients (41.38%) had stage II, 20 patients (34.48%) had stage III, and 5 patients (8.62%) had stage IV. As a result of mutational analysis, EGFR mutations on exon 20 were detected in 13 patients (22.41%, G→A transitions). EGFR mutations on exon 18, 19 and 21 were not detected. EGFR mutation increased in the earlier stage and the absence of lymph node metastasis (P=0.028). Conclusion: The incidence of EGFR mutation in Korean colorectal cancer patients is 22.41%. In addition, EGFR mutation significantly increased in the earlier stage and the absence of lymph node metastasis.

황토와 생석회의 혼합비율에 따른 축산폐수 처리에 관한 연구

김보국,신은섭,원찬희 全北大學校 附設 都市및環境硏究所 1999 都市 및 環境硏究 Vol.14 No.1

This study was conducted in order to investigate removal efficiency of turbidity, organic matter, nitrogen and phosphorus to reduce environmental pollution of treatment livestock wastewater with mixed proportion of loess and lime. The result of study, the removal of turbidity, COD_Cr and T-P increased along the dosage, and was to be fixed above the appropriate dosage. In the removal turbidity, the appropriate mixed proportion of loess and lime was Loess:Lime = 3:7, the appropriate dosage was 30g. In the removal COD_Cr, T-P and TKN was Loess:Lime = 3:7, 30g, Loess:Lime = 5:5, 20g, Loess:Lime = 5:5, 40g, respectively.

벡터형 공간객체 중심의 인터넷 원격 동영상 지도 서비스에 대한 실증적 고찰

엄정섭,이보미 한국지리정보학회 2003 한국지리정보학회지 Vol.6 No.2

벡터형 공간 객체는 장거리에 걸쳐 존재하고 있으며 폭이 좁은 특성을 가지고 있다. 벡터형 공간 객체에 대한 영상지도를 제작하기 위해서는 통상의 면형 영상지도에서 사용되는 기법과 전혀 다른 접근이 필요하다. 벡터형 공간 객체의 이와 같은 특성을 고려하여 원격동영상과 인터넷 GIS 기술을 결합한 영상지도 제작기법이 개발되었다. 인터넷상에서 원격동영상과 수치지도의 결합은 지형경관의 실제 모습을 시각적으로 제시할 수 있는 등 두 가지 기술의 장점을 효율적으로 활용할 수 있다. 원격 동영상지도는 사용자가 다양한 검색 방법에 의거 광역 공간정보를 단시간에 체험할 수 있는 기회를 제공하였으며 전통적인 지도는 위치 정보를 제공하여 동영상에 나타나는 물체가 실시간대에 확인할 수 있도록 하였다. 동영상 지도는 전통적인 지도학에서 확립된 지도의 개념 자체에 대한 재정립을 요구하게 되는 바, 본 연구는 ‘벡터형 공간 객체에 대한 인터넷 원격 동영상 지도’라는 새로운 개념을 제시하였다는 데 가장 큰 의의가 있다. 향후 본 시스템이 도입되어 본격적으로 서비스 될 경우 주민들에게 벡터형 공간 객체에 대한 다양한 유형의 영상정보-다른 축척, 다양한 응용분야-를 제공하여 지리정보의 대중화를 더욱 촉진할 수 있을 것으로 사료된다. The vector shaped object is generally very long (hundreds of thousands of kilometers) and very narrow (10-100 meters). Image mapping techniques and tools for these objects should be totally different from the traditional area-based targets. Acknowledging these unique characteristics of the vector shaped object, a motion picture mapping system has been developed by combining internet GIS technology with airborne video. In particular, integration between airborne video and digital maps took advantage of each component, and enabled the landscape structure to be visualized, interacted with and deployed all on the Web. The motion picture maps provided a completely new means for disseminating information for area-wide landscape in a visual and interactive manner to the general public while digital map with location information revealed successfully the major parameters that influence an area-wide spatial structure in the study area. The remote video approach breaks down the usual concept of image mapping in a conventional cartography. As a result, the research findings have established the new concept of 'internet airborne video mapping for vector shaped object', proposed as an initial aim of this paper. It would play a crucial role in improving the quality of public information service if the mapping system is operationally introduced into the Government since the highly user-friendly moving picture provides a completely new means for disseminating spatial information for vector shaped object.

흰쥐 경동맥 풍선손상 모델에서 리포좀 전달체를 이용한 에스트로겐의 국소전달 효과

윤영섭,장양수,정광회,박요석,김형관,강웅철,권진욱,정보영,조상호,김성순 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

경량 고강도 Mg-Zn계합금의 조직과 내식성에 관한 연구

안효준,남태현,임수근,안인섭,허보영,김기원 慶尙大學校 1995 論文集 Vol.34 No.1

The purpose in this study is to develope of high strength and corrosion reistant Mg-Zn system alloys by controlling microstructures and by adding of Cu elements. To produce optimum tesile strength,it is imperative to obtain effective grain refinment during casting. Optimal condition for grain size refining effect was obtained at the minmum composotion of 2wt% Cu or 1.5wt%So addition to Mg-6wt%Zn alloy.Age hardening behavior was experimented at the optimal compositions of the Mg-6wt%Zn, Mg-6wt%Zn-2wt%Cu and Mg-6wt%Zn-1.5wt%Si. The hardeness increment due to age hardening was at the Mg-Zn-Si alloy system. The improvement of corrosion resistanece by the addition with Cu is related to and increasing Zn/Cu ration in the intermatallic particles in the Mg-Zn system.Further improvements obtained by T6 treatment are related to the properties of the Mg-Zn phase,whih acts as a corrosion barrier by precipitating along the grainboundaries.Filiform corrosion observed soon after pit initiation and cellular form of pit propagation later in chloride media are explained in terms of solid solution matrix structure and the properties of air formed oxide.The information obtained from the fundamental research work is utilized in suggesting ways of improving the corrosion resistance of commercial AZ91.

적심 및 분지(分枝) 유도를 위한 신규 생장조절제로서의 mevalocidin 활용

김진석(Jin-Seog Kim),연규환(Gyu Hwan Yon),김보관(Bo Gwan Kim),최정섭(Jung-Sup Choi),김은애(Eun Ae Kim),고영관(Young Kwan Ko),이일영(Ill Young Lee) 한국잡초학회·한국잔디학회 2020 Weed & Turfgrass Science Vol.9 No.1

현재 적심 및 분지조절제로서 여러 가지 약제가 개발되어 사용되고 있지만 화합물마다 약해, 효능 부족, 심한 잔류성, 좁은 사용범위, 기타 부작용 등의 단점이 있기 때문에 이를 보완할 수 있는 보다 안전하고 새로운 유형의 물질 탐색과 개발은 매우 필요한 상황이다. 본 연구에서는 미생물 유래의 천연물인 mevalocidin에 대해 적심제 및 분지생장조절제로서의 사용 가능성을 검토하였다. 토마토, 고추, 콩, 고구마, 들깨 유묘를 대상으로 여러 농도의 mevalocidin을 처리해 본 결과, 흡수된 mevalocidin은 정단부로 빠르게 이동하여 생장점을 고사시키는 특징을 보이며 상대적으로 체내 대사가 빠르게 진행되는 것 같았다. 작물에 따라 적정농도가 다르지만 전반적으로 2-4 kg ha<SUP>-1</SUP> 처리범위에서 양호한 적심효과가 나타났고, 1-3 kg ha<SUP>-1</SUP> 처리 범위에서는 정단 생장억제와 함께 분지가 유도되면서 유도된 분지의 양호한 생장이 진행되었다. 따라서 미생물 유래의 천연물인 mevalocidin은 원예작물, 식량작물, 일부 수목에 적심 및 분지(分枝) 유도를 위한 친환경적 신규 생장조절제로서 유용하게 활용될 수 있을 것으로 판단되었다. Modulating shoot architecture in many agricultural, horticultural, and ornamental industries, is important in increasing the yield and quality of their products. This study was conducted to investigate the possibility to use mevalocidin as a new plant growth regulator for thinning and lateral shoot induction. Mevalocidin which was discovered from static cultures of two fungal isolates by Dow Agrosciences, were sprayed with various rates at the seedlings of five crops (tomato, pepper, soybean, sweet potato, and perilla) and investigated several cultural traits such as shoot growth, lateral shoot induction and length. As a results, mevalocidin was seemed to rapidly move into shoot tip and selectively killed an apical meristem part or inhibit its growth at a proper dosage. Then the induced-lateral shoots were grown rapidly, showing that the chemical was easily metabolized in plant. Generally mevalocidin at 2-4 kg ha<SUP>-1</SUP> (500- 1,000 ppm) led effectively to kill the apical part although the proper rate was different to crop species. On the other hand, The lower rate of mevalocidin (1-3 kg ha<SUP>-1</SUP>, 250-750 ppm) were effective for height control and lateral branching through the break of apical dominance. Therefore, it seemed that mevalocidin, natural phytotoxin from microorganism metabolites, could be used as a new eco-friendly plant growth regulator for thinning and lateral shoot induction for agricultural, horticultural, and ornamental industries.

Phytochemical Investigation of Acanthopanax koreanum

Bo Sup Chung,Young Ho Kim 한국생약학회 1989 생약학회지 Vol.20 No.2

As parts of systematic investigation of Korean medicinal plants, we have carried out phytochemical studies of Acanthopanax koreanum Nakai (Araliaceae) which grows in Jeju island indigenously. The root and stem barks of Acanthopanax species have been used as tonic and sedative agents in Korean traditional medicine. They are also applied to treat rheumatism and diabetes. In our earlier investigation of A. koreanum, several chemical components, comprising alignans, phenylpropanoids, polyacetylenes, and fatty acid methyl esters, were isolated from the root and stem barks. In this present communication, we report the structures of six diterpene compounds, including two novel pimaradienetype substances, from the diethyl ether and n-butyl alcohol extracts of root barks by silica gel column chromatography. The structures of the two new diterpenoids were elucidated as(-)-pimara-9(11), 15-dien-19-oic acid (2) and (-)-pimara-9(11), 15-dien-19-ol-19 acetate (3) by comical and spectroscopic techniques. The other known diterpene derivatives, (-)-pimara-9(11), 15-dien-19-ol (1), (-)-pimara-9(11), 15-diene (4), (-)-kaur-16-en-19-oic acid (5), and ent- 16β, 17-dihydroxy-(-)-kauran-19-oic acid (6) were structurally identified by comparison of their spectral data with those of authentic compounds.

Studies on the Constituents of Acanthopanax koreanum

Bo Sup Chung,Young Ho Kim 한국생약학회 1986 생약학회지 Vol.17 No.1

Acanthopanax koreanum Nakai (Araliaceae) is a indigenous medicinal plant growing throughout Jeju-Do in Korea. The plant extract is used in rheumatism, tonic, paralysis and sedative agent. From the roots of A. koreanum, lignan compounds, a diterpenoid, and a polyacetylene compound were isolated and their structures were elucidated by using IR, PMR, CMR and MS spectral data.