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Binod Prasad Luitel,Taek Jong Lee,Tserendendev Oyuntugs,Won Hee Kang 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.5
A study was undertaken to investigate the optimum planting bed size and growth containers using masato soil on fresh yield of pepper cultivars in the production space of greenhouse at Hwacheon from June to October, 2009. Results showed that cultivars significantly affected on plant height, total fruit number, marketable fruit number (%) plant and total yield per plant whereas bed size significantly affected the plant height, marketable fruit number (%), marketable and non-marketable weight (g) per plant. Planting transplants at 60㎝ bed width gave the highest total fruit number (61.8), marketable fruit number (58.3%) and weight (1,119.4 g) per plant. Growth containers significantly affected the total fruit number, marketable and non-marketable fruit number (%) and total fruit yield (g) per plant. ‘Chungyang’ and ‘Nokkwang’ grown on plastic pot gave higher total fruit number, marketable fruit number percentage and total yield per plant but ‘Special’, ‘Minipaprika’ and ‘Pimiento’ grown in Styrofoam trays significantly gave higher total fruit (no), marketable fruit number (%) and total yield (g) per plant. Hence, in masato soil, 60cm planting bed width in Styrofoam trays can be used to obtain better yield in peppers as well as to optimize the production space in plastic house.
Binod Prasad Luitel,Prakash Babu Adhikari,윤철수,강원희 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.2
This study was carried out to investigate the yield and fruit quality of tomato (Lycopersicon esculentum Mill.)cultivars using different plant bed width and growing substrates in Hwacheon Farm, 2011. Transplants of tomato cultivars ‘Campari’, ‘Temptation’, ‘Annamay’, and ‘Adoration’ were planted at 20 cm, 40 cm, 60 cm, and 80 cm bed width and same cultivars were grown at cocopeat, rockwool and masato in early July, 2011 in a greenhouse. Planting bed size significantly affected the fruit number, marketable fruit number (%) and weight (g), and total yield (g) per plant. Plants grown at 60 cm bed width yielded the highest fruit number (22.4), marketable fruit number (56.0%), marketable fruit weight (356.8 g) and total yield (564.5 g) per plant. Bed width had no significant effect on fruit size and quality characters. Plants grown in cocopeat produced the highest marketable fruit number (56.2%) per plant and yielded the greatest (445.6g) marketable yield per plant. Plants grown in cocopeat substrate produced higher fruit number (5.2%) and total yield (0.7%) than that of rockwool substrate. Fruit size and fruit quality characters showed no significant differences within growing substrates. Hence, planting the tomato cultivars in single row at 60 cm bed width is better approach to optimize the production space in greenhouse and cocopeat is recommended as potential growing substrates for tomato cultivation to increase its yield and fruit quality characters. This study was carried out to investigate the yield and fruit quality of tomato (Lycopersicon esculentum Mill.)cultivars using different plant bed width and growing substrates in Hwacheon Farm, 2011. Transplants of tomato cultivars ‘Campari’, ‘Temptation’, ‘Annamay’, and ‘Adoration’ were planted at 20 cm, 40 cm, 60 cm, and 80 cm bed width and same cultivars were grown at cocopeat, rockwool and masato in early July, 2011 in a greenhouse. Planting bed size significantly affected the fruit number, marketable fruit number (%) and weight (g), and total yield (g) per plant. Plants grown at 60 cm bed width yielded the highest fruit number (22.4), marketable fruit number (56.0%), marketable fruit weight (356.8 g) and total yield (564.5 g) per plant. Bed width had no significant effect on fruit size and quality characters. Plants grown in cocopeat produced the highest marketable fruit number (56.2%) per plant and yielded the greatest (445.6g) marketable yield per plant. Plants grown in cocopeat substrate produced higher fruit number (5.2%) and total yield (0.7%) than that of rockwool substrate. Fruit size and fruit quality characters showed no significant differences within growing substrates. Hence, planting the tomato cultivars in single row at 60 cm bed width is better approach to optimize the production space in greenhouse and cocopeat is recommended as potential growing substrates for tomato cultivation to increase its yield and fruit quality characters.
Binod Prasad Luitel,강원희 한국원예학회 2013 Horticulture, Environment, and Biotechnology Vol.54 No.2
The in vitro androgenic response of minipaprika F1 hybrid cv. Vine sweet (red, yellow, and orange form)was investigated using Dumas de Vaulx (CP) and Murashige and Skoog (MS) culture media to ascertain the effectiveness of androgenesis in haploid production. The frequency of callus and embryo formation varied in anther cultures of all minipaprika forms in both culture media. Anthers in both culture media responded to form the callus without regeneration in all minipaprika forms. The anthers of ‘Vine sweet-yellow’ produced 62.5% and 46.7% normal embryo in CP and MS medium, respectively. ‘Vine sweet-yellow’ produced almost four fold more normal-looking embryos than ‘Vine sweet-red’ in both culture media, and ‘Vine-sweet orange’ showed low androgenic response to anther culture. The anthers cultured in CP medium gave more normal embryo in all minipaprika forms as compared to MS medium. Among a total of 51 embryos transferred to growth regulator free MS medium for regeneration, 45plants were regenerated. Flow cytometry analysis revealed that 44.5%, 42.4%, and 33.3% plants were haploids, and 55.5%, 57.6%, and 66.7% plants were spontaneous diploids in red, yellow and orange forms, respectively. The chromosome number of haploid plant was 12 and that of spontaneous diploid was 24. Stomata characters and chloroplast count in the guard cell of leaf stomata were also found to be reliable and simple method to analyze the ploidy level of regenerated plants. The spontaneous diploids confirmed as doubled haploids (DHs) following the self-pollination. The obtained DH and haploid plants from anther culture would be the valuable breeding materials for heterosis breeding.
Characterization of a Chalcosyltransferase (gerGTII) in Dihydrochalcomycin Biosynthesis
Binod Babu Pageni,오태진,Ta Thi Thu Thuy,송재경 한국분자세포생물학회 2008 Molecules and cells Vol.26 No.3
An open reading frame, designated GerGTII and located downstream of the polyketide synthase genes, has been identified as a chalcosyltransferase by sequence analysis in the dihydrochalcomycin biosynthetic gene cluster of Streptomyces sp. KCTC 0041BP. The deduced product of gerGTII is similar to several glycosyltransferases, authentic and putative, and it displays a consensus sequence motif that appears to be characteristic of a sub-group of these enzymes. Specific disruption of gerGTII within the S. sp. KCTC 0041BP genome by insertional in-frame deletion method, resulted complete abolishment of dihydrochalcomycin and got the 20-O-mycinosyl-dihydrochalconolide as intermediate product in dihydrochalcomycin biosynthesis which was confirmed by electron spray ionization-mass spectrometry and liquid chromatography-mass spectrometry. Dihydrochalcomycin also was recovered after complementation of gerGTII.