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Binling Ai,Jianzheng Li,Xue Chi,Jia Meng,Ajay Kumar Jha,Chong Liu,En shi 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.4
This study was conducted to identify theoptimum pH range and the appropriate buffer for butyricacid production from rice straw by fermentation using anundefined mixed culture. A series of experiments conductedat pH levels of 5.0 ~ 7.0 showed that neutral pH improvedrice straw conversion and consequently carboxylic acidproduction. The highest butyric acid production (up to6.7 g/L) was achieved at pH of 6.0 ~ 6.5, while it was only1.7 g/L without pH control or at pH 5.0. Another series ofexperiments conducted at pH 6.0 ~ 6.5 buffered withCaCO3, NaHCO3, NH4HCO3 and their combinationsindicated that different buffers had different effects ontheproduct spectrum, and that CaCO3 combined with NaHCO3was an effective buffer for butyric acid production. Thehighest total volatile fatty acids (about 12.6 g/L) productionand one of the two highest butyric acid concentrations(about 7.6 g/L) were obtained by buffering with CaCO3combined with NaHCO3. PCR-DGGE analysis revealedthat different pH and buffers also influenced the microbialpopulation distribution. Bacteria were suppressed at lowpH , while the bacterial community structures at higher pHvaried slightly. Overall, this study presents an alternativemethod for butyric acid production from lignocellulosicbiomass without supplementary cellulolytic enzyme.
Butyric Acid Fermentation of Sodium Hydroxide Pretreated Rice Straw with Undefined Mixed Culture
( Binling Ai ),( Jianzheng Li ),( Xue Chi ),( Jia Meng ),( Chong Liu ),( En Shi ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.5
This study describes an alternative mixed culture fermentation technology to anaerobically convert lignocellulosic biomass into butyric acid, a valuable product with wide application, without supplementary cellulolytic enzymes. Rice straw was soaked in 1% NaOH solution to increase digestibility. Among the tested pretreatment conditions, soaking rice straw at 50°C for 72 h removed ~66% of the lignin, but retained ~84% of the cellulose and ~71% of the hemicellulose. By using an undefined cellulose-degrading butyrate-producing microbial community as butyric acid producer in batch fermentation, about 6 g/l of butyric acid was produced from the pretreated rice straw, which accounted for ~76% of the total volatile fatty acids. In the repeated-batch operation, the butyric acid production declined batch by batch, which was most possibly caused by the shift of microbial community structure monitored by denaturing gradient gel electrophoresis. In this study, batch operation was observed to be more suitable for butyric acid production.
Weakly almost periodic points and chaotic dynamics of discrete amenable group actions
Bin Ling,Xiaoxiao Nie,Jiandong Yin 대한수학회 2019 대한수학회지 Vol.56 No.1
The aim of this paper is to introduce the notions of (quasi) weakly almost periodic point, measure center and minimal center of attraction of amenable group actions, explore the connections of levels of the orbit's topological structure of (quasi) weakly almost periodic points and study chaotic dynamics of transitive systems with full measure centers. Actually, we showed that weakly almost periodic points and quasi-weakly almost periodic points have distinct orbit's topological structure and proved that there exists at least countable Li-Yorke pairs if the system contains a proper (quasi) weakly almost periodic point and that a transitive but not minimal system with a full measure center is strongly ergodically chaotic.
Bin Ling,Yao Chen,Zhiyun Wu,Zhichang Zhao,Juan Wu,Yingping Cao 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.4
The intestinal tract may be an important reservoir for antibioticresistant genes [1]. Determining the prevalence of quinolone resistance in intestinal bacteria within the community is important for both healthy subjects and hospital patients as quinolone is one of the most commonly used antibiotics in China. The prevalence of plasmid-mediated quinolone resistance (PMQR) geneharboring Enterobacteriaceae in the gut flora among healthy humans was previously unknown, so we assessed the prevalence of PMQR genes among commensal Escherichia coli in healthy persons from one region in China. This study was approved by the Ethics Committee of the Fujian Medical University Union Hospital (No. 2012KY085). Written informed consent was obtained from all participants.