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Bikash Bhusal,Un Taek Lim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10
Frankliniella intonsa (Trybom) (Thysanoptera: Thripidae) is an important pest in protected culture. We assessed fourrepellents (carvacrol, cis-jasmone, methyl jasmonate, and methyl salicylate) of Frankliniella occidentalis (Pergande)(Thysanoptera: Thripidae) known from literatures to develop a behavioral management tactic against F. intonsa. We measuredthe repellency of F. intonsa that exposed to 100 times diluted repellents on leaf disc. Repellency was 2.60 times higherin methyl jasmonate followed by methyl salicylate (2.16 times), cis-jasmone (2.15 times) and carvacrol (2.08 times) comparedto control. These results suggest that methyl jasmonate can be incorporated as a component of integrated managementof F. intonsa.
혼합 폐 전지로부터 용매추출을 이용하여 회수된 Zn와 Mn Cd 용액 내 Zn power를 이용한 Cd의 제거
( Bikash Ranjan Das ),주성호,신동주,오창현,왕제필,신선명 한국폐기물자원순환학회(구 한국폐기물학회) 2014 한국폐기물자원순환학회 춘계학술발표논문집 Vol.2014 No.-
폐 전지로부터 유가금속을 회수하는 연구는 환경적인 측면뿐만 아니라 광석 보다 높은 품위의 유가금속을 함유하고 있기 때문에 경제적인 측면에서도 상당히 중요하다. 따라서 본 연구는 폐 혼합전지 내 유가금속 중 Mn과 Zn의 회수와 Cd의 제거에 대한 연구를 수행하였다. 폐 혼합전지의 조성은 질량비로 40%알카라인·망간전지, 30%리튬이온전지, 10% 리튬 1차전지, 10% 니켈-카드뮴 전지, 10% 니켈 수소전지를 혼합하였다. 이로부터 물리적 전처리 및 불순물의 제어 후 Zn와 Mn 그리고 Cd을 동시에 회수하기 위한 용매추출 연구를 수행하였다. 그 결과 최적 조건은 용매인 D2EHPA 농도 27%(vol.), 비누화도 45%, O/A=2에서 Zn와 Mn 그리고 Cd을 전량 회수할 수 있었고, 추출 및 탈거 후 탈거용액 내 Zn power를 투입하여 Cd을 4ppm까지 제어 가능하였다. 따라서 이 연구로부터 혼합전지 내 Zn와 Mn을 동시에 회수할 수 있는 최적 공정을 제시할 수 있을 것이다.
Evaluation of chemical attractants on Frankliniella intonsa (Thysanoptera: Thripididae)
Bikash Bhusal,Yong Jun Yu,Un Taek Lim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.04
Frankliniella intonsa (Trybom) (Thysanoptera: Thripididae) is an important pest of field and greenhouse crops such as pepper and strawberry. Chemical attractants are commonly used for monitoring thrips, but few are available for F. intonsa. Thus, we evaluated three attractants of Frankliniella occidentalis known from literatures for their attraction of F. intonsa in Y-tube olfactometer. Fed unmated adult females of F. intonsa showed higher initial response to (S)-(−)-verbenone than other attractants, i.e. aggregation pheromone of F. occidentalis and methyl isonicotinate. Unfed mated adult females of F. intonsa showed similar initial response. These results suggest that (S)-(−)-verbenone also can be used for monitoring and further in behavioral control tactics of F. intonsa.
Bikash Thapa,Dong-Gyu Kim,Seongwon Pak,Dohyeon Chung,Jungwoo Shin,Keunwook Lee 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Activated B cells in the germinal center (GC) undergo clonal expansion along with immunoglobulin isotype switching and affinity maturation, leading to differentiation into plasma cells that produce appropriate humoral immunity against invading pathogen. Although PI3K/mTOR signaling is indispensable for survival and proliferation of B cells, little is known about how this signaling pathway integrates environmental milieu within the GCs to regulate GC reactions and to yield plasma cells. In this study, we dissected the impact of PI3K/mTOR signaling pathway on antibody responses by combination of the inducible deletion of Pten (a PI3K-antagonistic lipid phosphatase), Raptor (a subunit of mTORC1), and Rictor (a subunit of mTORC2). Rag2-/- mice were reconstituted with naïve B cells isolated from the inducible KO mice along with normal CD4 T cells and then immunized with NP-ovalbumin. Mice that received Pten depleted B cells exhibited impaired class switching to IgG1 and production of high affinity antibodies against the NP antigen in the sera. On the other hand, inactivation of mTORC2 by depleting Rictor under Pten loss in B cells rescued IgG1 class switching and affinity maturation of NP-specific antibodies whereas that of mTORC1 by depletion Raptor did not. To delineate the mechanism, we adapted a co-culture system to generate GC B cells and plasmablasts (PBs) ex vivo. B cells depleted Pten differentiated into PBs expressing predominantly IgM and less IgG1 in the co-cultivation which was comparable to the control PBs. Finally, we performed transcriptomic analysis of PB cells and mapped to the set of B cell function-associated genes under the regulation of the PI3K-PTEN-mTORC2 signaling network. It is in progress to determine whether this signaling modulation within B cell compartment is required for protective antibody responses against influenza virus. Collectively, out data provide a mechanistic insight by which PTEN and mTORC2 tune up the PI3K signaling pathway to promote class-switching and affinity maturation of antibodies during GC reaction.
( Bikash Bogati ),( Nirajan Bhusal ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Bloodstream infections in neonates are life-threatening emergencies.Identifi cation of the common bacteria causing such infections and their susceptibility patterns will provide necessary information for timely intervention. This study is aimed at determining the susceptibilities of bacterial etiological agents to commonly-used antimicrobial agents for empirical treatment of suspected neonatal sepsis in tertiary care center of Nepal. Methods: A prospective study was carried out at Dhulikhel Hospital- Kathmandu University Hospital, Nepal from July 2013 to January 2014 among neonates with preliminary diagnosis of sepsis sent for blood culture. Results: Out of 340 blood specimens cultured, the prevalence of confi rmed bacterial sepsis was 16.47% (56/340) where 15% (24/160) were male and 17.7% (32/180) were female baby. Among the specimen, almost same rate of skin contaminants were detected (16%), whereas 67.08% had no growth. Gram Positive Cocci (GPC) were the predominant isolates with 92.9% (52/56) with Staphylococcus aureus accounting for 46.4% of the total isolates followed by Enterococcus (28.5%), Coagulase-Negative Staphylococci (14.3%) and Group B Streptococcus (3.6%). Gram Negative Rods (GNR) comprised only 7.1% (4/56) of all isolates with E.coli and Salmonella being the organisms isolated. GPC showed 100% resistance to Ampicillin and 88.8% resistanceto Amoxycillin-Clavilunate. However, they showed 100% sensitivity to Vancomycin and 92.5%, 88.8%, 81.4%, 74.07% susceptibility to Amikacin, Gentamycin, Ciprofi oxacin and Cefotaxime respectively. Among GNR, E. coli was resistant to Ampicillin and Cefi pime, whereas S. Typhi was with decreased susceptibility to ciprofi oxacin and completely resistant to Nalidixic acid.Conclusions: Coagulase Negative and Coagulase Positive Staphylococci, GBS, E. coli and S. Typhi were the etiological agents of neonatal sepsis in this hospital.GPC were resistant to Ampicillin and Amoxycillin-Clavulanic acid and sensitive to Vancomycin, Amikacin and Gentamycin.
D-10 : 폐 혼합전지로부터 시간에 따른 과분쇄를 통한 유가금속의 농축
( Bikash Ranjan Das ),주성호,신동주,신선명 한국폐기물자원순환학회(구 한국폐기물학회) 2013 한국폐기물자원순환학회 추계학술발표논문집 Vol.2013 No.-
폐 전지로부터 유가금속을 회수하는 연구는 환경적인 측면뿐만 아니라 전지 내 광석 보다 높은 품위의 유가금속을 함유하고 있기 때문에 경제적은 측면에서도 상당히 중요하다. 따라서 본 연구는 폐 전지로부터 유가금속을 회수하기 위해 전처리 공정에서 불순물인 Fe및 Al을 물리적 전처리 과정을 통하여 제거하고 유가금속을 농축하기 위한 연구를 수행하였다. 폐 혼합전지의 조성은 질량비로 40%알카라인·망간 전지, 30%리튬이온전지, 10% 리튬 1차전지, 10% 니켈-카드튬 전지, 10% 니켈 수소전지로써 혼합하였다. 혼합된 폐 전지 내 유가금속의 함량은 각 각 14.45% Fe, 7.79% Al, 2.28% Cu, 1.814% Cd, 8.1% Zn, 9.27% Mn, 8.02% Co, 8.72% Ni, 1.43% Li으로 구성되었다. 실험과정은 열처리, 파·분쇄, 시간에 따른 단체분리 과정으로 진행하였다. 단체 분리시 사용된 mesh는 5, 7, 10, 20, 40, 50, 70 mesh size를 사용하였고 그 결과 20초 과·분쇄 5 mesh 기준으로 Zn, Mn, Co, Ni, Li의 경우 농축율이 각 각 90.83%, 92.82%, 91.87%, 92.61%, 87.07% 달성되었고 불순물인 Fe, Al Cu, Cd가 각 각 83.3%, 82.19%, 27.00%, 16.12% 제거되었다. 따라서 이 후 습식 공정을 응용하여 유가 금속을 분리 및 회수하기 위한 최적의 시료 조성을 획득하였다.