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Yasmin Khambhaty,Bhavanath Jha,Kalpana Mody 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.5
A marine, gram positive, aerobic, spore forming, and non flagellated bacterium which degrades low melting point (LMP) -agarose was isolated from the west coast of India and identified as Bacillus megaterium based on its mor-phological, biochemical, and molecular characterization. This bacterium produced clear haloes or zone of clearance on agar containing plates which was a clear indication of its agarolytic property. The extracellular agarase thus ob-tained was purified 8.8 and 78 fold from the culture supernatant by ammonium sulfate precipitation and gel filtration, respectively. Molecular mass by gel filtration and SDS-PAGE gave values of 15 and 12 kDa, respectively. The opti-mum temperature and pH for maximum agarase activity were 40C and 6.6. The activity of agarase was drastically reduced by addition of metal ions in the assay system. This agarase, gave a Km and Vmax value of 4 mg/mL and 2.75 μmol/min/mg. The isolation of protoplast from agarophyte like Gelidiella acerosa using indegenous agarase is re-ported for the first time.
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Dinkar Singh,Bhavanath Jha 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.4
The small, non-coding RNA species (20–24nucleotides), including microRNAs (miRNAs) and smallinterfering RNAs, are key regulators of gene expression ineukaryotic cells. Mostly, miRNAs have been identifiedfrom glycophytes under different abiotic stresses. Theisolation and identification of miRNAs from halophytes arechallenging due to the presence of high salt concentrations. In this study, small RNA cDNA libraries were constructedusing an improved method to identify salt-responsive novelmiRNAs from Salicornia brachiata. A total of 159sequences were cloned and analysed. There was no adaptercontamination and low-abundance small RNAs wereamplified efficiently. Twelve putative miRNAs/pre-miRNAsand a small RNA were identified and confirmed bynorthern blot. Among the putative miRNAs, nine are noveland three belong to the conserved miRNA families(MIR169g, MIR1433, MIR138b). Northern hybridisationand real-time PCR showed that nine miRNAs (seven noveland two conserved) and one small RNA were highlyexpressed at 2.0 M NaCl, two novel miRNAs at 1.5 MNaCl, and one conserved miRNA at 1.0 M NaCl. Furthermore,67 putative target genes of the isolated miRNAwere predicted on the basis of sequence homology, ofwhich more than 50 % are associated with abiotic stresses. The target genes of miRNA 169g (Sb-miRNA10), and SbmiRNA7(NF-YA transcription factor and cytochromeP450-like TATA box binding protein respectively) wereanalysed by real-time PCR. The study of miRNAs from anextreme halophyte broadens our understanding of theimportant role played by miRNAs in plant abiotic stress.
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Mandal, Subir Kumar,Joshi, Vithaldas Hemantkumar,Bhatt, Devabratta Chandrashanker,Jha, Bhavanath,Ishimaru, Takashi The Korean Society of Phycology 2006 ALGAE Vol.21 No.4
Amphora costata W. Smith 1853 is a down thrown diatom species and also known as metal corrosive ship-fouling organism. A. costata was isolated from Alang ship breaking yard, Alang and evaluated the toxicity tolerance and growth responses of the cultures exposed to different doses of toxic and relatively accessible heavy metals, such as Fe, Mn, Cd, Co, Cu, Zn, Ni, and Pb in the constantly monitored laboratory culture conditions. The strongest toxic effect was observed on A. costata exposed to Cd even at relatively low concentrations as compared to other metals. The following trend of decreasing order of toxicity i.e. Cd>Zn>Ni>Co>Pb>Cu>Fe was observed, when they were exposed to equal concentration and expose time.
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