Differential Expression of Genes Associated with Endomembrane Transport and Cell Wall Metabolism during Ripening of ‘Chimarrita’ and ‘Granada’ Peach

Camila Pegoraro,Roberta Manica-Berto,F?bio Clasen Chaves,Carolina Terra Borges,Jader Job Franco,Cesar Valmor Rombaldi,Jorge Adolfo Silva 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.3

Expression of genes involved in endomembrane transport and cell wall metabolism, particularly in synthesis and degradation of cell wall macromolecules were compared during the ripening of a white melting flesh cultivar, Chimarrita and a yellow non-melting flesh cultivar, Granada in order to evaluate their association with fruit softening. In both, most genes were up-regulated as ripening evolved. In ‘Chimarrita’, overall higher transcript accumulation occurred at 95 days after anthesis, while in ‘Granada’ higher relative transcription was detected at 115 days after anthesis. Although transcript accumulation was correlated with fruit softening, the process does not seem to be totally dependent on the set of studied genes, since the peak of RNA abundance occurred when flesh firmness had already decreased.

Mixed mode fracture assessment of U-notched graphite Brazilian disk specimens by means of the local energy

A.R. Torabi,F. Berto 국제구조공학회 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.50 No.6

A fracture criterion based on the strain energy density (SED) over a control volume, which embraces the notch edge, is employed in the present paper to assess the fracture loads of some U-notched Brazilian disk (UNBD) specimens. The specimens are made of commercial graphite and have been tested under pure mode I, pure mode II and mixed mode I/II loading. The results show that the SED criterion allows to successfully assess the fracture loads of graphite specimens for different notch tip radii and various mode mixity conditions with discrepancies that fall inside the scatter band of ±20%.

Application the mechanism-based strain gradient plasticity theory to model the hot deformation behavior of functionally graded steels

Salavati, Hadi,Alizadeh, Yoness,Berto, Filippo Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.51 No.4

Functionally graded steels (FGSs) are a family of functionally graded materials (FGMs) consisting of ferrite (${\alpha}$), austenite (${\gamma}$), bainite (${\beta}$) and martensite (M) phases placed on each other in different configurations and produced via electroslag remelting (ESR). In this research, the flow stress of dual layer austenitic-martensitic functionally graded steels under hot deformation loading has been modeled considering the constitutive equations which describe the continuous effect of temperature and strain rate on the flow stress. The mechanism-based strain gradient plasticity theory is used here to determine the position of each layer considering the relationship between the hardness of the layer and the composite dislocation density profile. Then, the released energy of each layer under a specified loading condition (temperature and strain rate) is related to the dislocation density utilizing the mechanism-based strain gradient plasticity theory. The flow stress of the considered FGS is obtained by using the appropriate coefficients in the constitutive equations of each layer. Finally, the theoretical model is compared with the experimental results measured in the temperature range $1000-1200^{\circ}C$ and strain rate 0.01-1 s-1 and a sound agreement is found.

Effect of Glutamine, Glutamic Acid and Nucleotides on the Turnover of Carbon (δ¹³C) in Organs of Weaned Piglets

Amorim, Alessandro Borges,Berto, Dirlei Antonio,Saleh, Mayra Anton Dib,Telles, Filipe Garcia,Denadai, Juliana Celia,Sartori, Maria Marcia Pereira,Luiggi, Fabiana Golin,Santos, Luan Sousa,Ducatti, Carl Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.8

Morphological and physiological alterations occur in the digestive system of weanling piglets, compromising the performance in subsequent phases. This experiment aimed at verifying the influence of glutamine, glutamate and nucleotides on the carbon turnover in the pancreas and liver of piglets weaned at 21 days of age. Four diets were evaluated: glutamine, glutamic acid or nucleotides-free diet (CD); containing 1% glutamine (GD); containing 1% glutamic acid (GAD) and containing 1% nucleotides (ND). One hundred and twenty-three piglets were utilized with three pigs slaughtered at day zero (weaning day) and three at each one of the experimental days (1, 2, 4, 5, 7, 9, 13, 20, 27, and 49 post-weaning), in order to collect organ samples, which were analyzed for the ${\delta}^{13}C$ isotopic composition and compared by means of time. No differences were found (p>0.05) among treatments for the turnover of the $^{13}C$ in the pancreas ($T_{50%}$ = 13.91, 14.37, 11.07, and 9.34 days; $T_{95%}$ = 46.22, 47.73, 36.79, and 31.04 days for CD, GD, GAD, and ND, respectively). In the liver, the ND presented accelerated values of carbon turnover ($T_{50%}=7.36$ and $T_{95%}=24.47days$) in relation to the values obtained for the GD ($T_{50%}=10.15$ and $T_{95%}=33.74days$). However, the values obtained for the CD ($T_{50%}=9.12$ and $T_{95%}=30.31days$) and GAD ($T_{50%}=7.83$ and $T_{95%}=26.03days$) had no differences (p>0.05) among other diets. The technique of $^{13}C$ isotopic dilution demonstrated trophic action of nucleotides in the liver.

Mixed mode fracture assessment of U-notched graphite Brazilian disk specimens by means of the local energy

Torabi, A.R.,Berto, F. Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.50 No.6

A fracture criterion based on the strain energy density (SED) over a control volume, which embraces the notch edge, is employed in the present paper to assess the fracture loads of some U-notched Brazilian disk (UNBD) specimens. The specimens are made of commercial graphite and have been tested under pure mode I, pure mode II and mixed mode I/II loading. The results show that the SED criterion allows to successfully assess the fracture loads of graphite specimens for different notch tip radii and various mode mixity conditions with discrepancies that fall inside the scatter band of ${\pm}20%$.

Application the mechanism-based strain gradient plasticity theory to model the hot deformation behavior of functionally graded steels

Hadi Salavati,Yoness Alizadeh,Filippo Berto 국제구조공학회 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.51 No.4

Functionally graded steels (FGSs) are a family of functionally graded materials (FGMs) consisting of ferrite (α), austenite (γ), bainite (β) and martensite (M) phases placed on each other in different configurations and produced via electroslag remelting (ESR). In this research, the flow stress of dual layer austenitic-martensitic functionally graded steels under hot deformation loading has been modeled considering the constitutive equations which describe the continuous effect of temperature and strain rate on the flow stress. The mechanism-based strain gradient plasticity theory is used here to determine the position of each layer considering the relationship between the hardness of the layer and the composite dislocation density profile. Then, the released energy of each layer under a specified loading condition (temperature and strain rate) is related to the dislocation density utilizing the mechanism-based strain gradient plasticity theory. The flow stress of the considered FGS is obtained by using the appropriate coefficients in the constitutive equations of each layer. Finally, the theoretical model is compared with the experimental results measured in the temperature range 1000-1200°C and strain rate 0.01-1 s-1 and a sound agreement is found.

Communication between CAN Bus Control Devices and Virtual Machines

Richard Gagne,Vincent Remillard,Luca Berto,Lionel Pierreton 유공압건설기계학회 2017 유공압건설기계학회 학술대회논문집 Vol.2017 No.5

The fluid power industry has seen significant changes in the last decade, following the electrification of hydraulic technologies. By adding intelligence to hydraulic and pneumatic manufacturers’ components, designing and testing new fluid power applications require the additional expertise of control specialists. Since fluid power and control specialists use different CAE tools to create and virtually test their respective design, there is a need to create an integrated test environment to better communicate, understand and specify requirements of electro-hydraulic systems. In this paper, we will demonstrate how virtual components up to a complete machine developed on simulation software can be controlled by physical control devices (joystick, controllers…) using CAN bus communication. Examples of this will be illustrated for virtual systems built according to manufacturers’ specifications in Automation Studio™ and communicating with the physical controllers: a hydraulic simulation model (ex.: Eaton CMA valves) communicating with a programmable controller and multiple devices as well as Eaton HFX Controller, Danfoss PLUS+1<SUP>®</SUP> microcontroller or HydraForce CoreTek controller.

Structural response of corroded RC beams: a comprehensive damage approach

Irene Barbara Nina Finozzi,Luisa Berto,Anna Saetta 사단법인 한국계산역학회 2015 Computers and Concrete, An International Journal Vol.15 No.3

In this work, a comprehensive approach to model the structural behaviour of Reinforced Concrete (RC) beams subjected to reinforcement corrosion is proposed. The coupled environmental – mechanical damage model developed by some of the authors is enhanced for considering the main effects of corrosion on concrete, on composite interaction between reinforcement bars and concrete and on steel reinforcement. This approach is adopted for reproducing a set of experimental tests on RC beams with different corrosion degrees. After the simulation of the sound beams, the main parameters involved in the relationships characterizing the effects of corrosion are calibrated and tested, referring to one degraded beam. Then, in order to validate the proposed approach and to assess its ability to predict the structural response of deteriorated elements, several corroded beams are analyzed. The numerical results show a good agreement with the experimental ones: in particular, the proposed model properly predicts the structural response in terms of both failure mode and load-deflection curves, with increasing corrosion level.

Printing orientation influence on tensile strength of PA12 specimens obtained by SLS

Ivana Jevtić,Zorana Golubović,Goran Mladenović,Filippo Berto,Aleksandar Sedmak,Aleksa Milovanović,Miloš Milošević 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.11

Selective laser sintering (SLS) is one of the additive manufacturing technologies dedicated to the production of high-quality parts with complex geometries. Here, polyamide 12 (PA12) is a commonly used material, for manufacturing parts with sufficient mechanical and thermal properties. In SLS, many manufacturing parameters have an effect on the mechanical properties of final parts. Even the decision regarding the orientation of a part in a powder bed may have a significant effect on the mechanical properties. In this research, the influence of horizontal (H) and vertical (V) printing orientations on the ultimate tensile strength (UTS) of fabricated PA12 specimens are examined. Our research findings show that H specimens exhibit larger deformations and smaller UTS value scatter in comparison with V specimens. Also, worth pointing out is the fact that V-oriented specimens have a higher elastic modulus. One can assume that the sintering process is more effective in V specimens, due to a more uniform laser beam trajectory than in the H specimens’ case.

Mechatronic Challenges to Develop and Implement New Hydraulic Technologies

Richard Gagne,Vincent Remillard,Veronique Bader,Luca Berto 유공압건설기계학회 2016 유공압건설기계학회 학술대회논문집 Vol.2016 No.6

A new generation of hydraulic components with their integrated control capability, provides more precision and flexibility, but brings implementation challenges. To cope with this changing reality, the fluid power industry needs to redefine work processes surrounding mechatronic machine development, including the creation of training programs. Although a new generation of students accustomed with numerical simulation technologies is starting to emerge, their applied knowledge is often very limited. In addition, experienced specialists who possess this expertise are also getting scarce and harder to replace. To facilitate this technological transition, simulation and numerical analysis tools seem promising. However, to truly be effective, these tools must enable a collaborative work environment that will leverage the machine knowledge of everyone involved in the development process. The goal of this paper is to provide hydraulic engineers with an optimized and integrated approach, in-line with the working process evolution. This approach is demonstrated by two case studies of electrohydraulic independent-metering valves systems. The first one is the development of a hydraulic and control simulation environment of a CMA Eaton valve. The second one studies the interactions of a virtual Sun Hydraulic valve system that regulates the actuator movement under different loads, co-simulated with a PLC.