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        눈꽃동충하초(Paecilomyces tenuipes)의 분생자경속 형성

        박기범 외 한국버섯학회 2004 한국버섯학회지 Vol.2 No.2

        눈꽃동충하초 분생자경속을 형성하는데 액체배양을 이용한 접종원의 배지는 PDB가 가장 우수하였으며, 분생자경속 형성에 유리한 배양온도는 20℃로 나타났다. 조명은 500lx.에서 가장 양호한 성장을 하였으며 광조사 시간은 18hr이 가장 유리한 분생자경형성 조건을 나타내었다. 알맞은 배지 조건은 번데기와 현미를 50 : 50 의 비율에 증류수를 현미에 1 : 1비율로 첨가한 혼합배지에서 높게 나타났다. 번데기배지는 원형상태와 분쇄상태 중에서는 분쇄된 상태에서 번데기배지의 공극률이 높아 균사의 침입이 용이하여 분생자경속 회수율과 균사의 배지내에 형성이 원형상태의 번데기보다 더욱 높게 나타났으며, 균사의 생장도 역시 분쇄한 번데기에서 더욱 빠르고 고르게 나타났다. 종균 접종량은 15ml의 종균을 접종하면 분생자경속이 잘 형성되었다. 눈꽃동충하초 재배에 가장 안정하게 재배하려면 분쇄한 번데기에 현미와 혼합한 배지에 종균 15ml를 접종하여 24 에서 발이를 시킨 후 20℃의 배양실에서 재배하는 것이 가장 좋은 것으로 나타났다. Potato dextrose broth was the best inoculation medium to produce in vitro synnemata of Paecilomyces tenuipes. The optimum temperature for synnemata formation of P. tenuipes was 20℃ under 500 lx of light intensity. Highest synnemata production was observed at 18 hr of light period per day. The medium containing 50 g of each silkworm pupae and brown rice produced highest number of synnemata. The optimum ratio of brown rice to distilled water was found as 1:1. Mycelial growth and synnemata production of P. tenuipes was faster and higher in medium containing grinded pupae as compared to whole pupae. The optimum inoculum amount per bottle of medium was 15ml. The highest synnemata production of P. tenuipes was obtained by incubating rice pupae medium at 24℃ until mycelium grows sufficiently after inoculation and then transferring it to 20℃ chamber till harvest.

      • 객체지향 기법을 이용한 다중 스레드 프로그램 기법에 관한 연구

        송기범,박영옥,이준 조선대학교 동력자원연구소 1995 動力資源硏究所誌 Vol.17 No.2

        The Software which uses object-oriented method has the advantage of extensibility, independence and easy maintenance, but has the disadvantage of decrease of system performance. Object-oriented software provides parallel process of objects, and then to improve of system performance we categorize objects into active and passive ones and then use active one. Therefore we implement active and passive objects with object-oriented language, propose multi threaded programming method using thread library for real time process.

      • 품종 및 배지의 종류가 양액재배 무화과의 생장과 발육에 미치는 영향

        박순기,이범선,박용서,정순주 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

        무화과의 암면 양액재배를 통하여 시설재배의 가능성을 검토하고 배지종류 및 품종에 따른 과실생육 및 수량을 검토하여 우리나라 기후에 가장 알맞는 품종을 선발하고 육성을 도모하기 위하여 수행하였다. 봉래시 품종을 4종류의 배지(펄라이트, 코코피트, 암면슬라브 및 입상암면)에 재식하고, 또한 '마쓰이도후인', '봉래시', '화이트제노아' 및 '브라운터어키' 품종을 본엽 4∼5매인 5월 17일에 암면슬라브에 각각 15주씩 정식하여 생장반응과 총수량 등을 조사한바 배지종류에 따른 봉래시 품종의 생장반응은 암면과 입상암면 배지에서 초장, 엽수, 엽장 및 측지수가 다른 배지보다 높게 나타나 이들 두가지 배지의 이용 가능성이 컸고, 암면을 사용하여 재배한 경우 초기 생장은 봉래시 품종이 좋았으나 과실의 수량면에서 단위면적당 총수량과 주당 평균수량은 마쓰이도후인>브라운터어키>봉래시, 화이트제노아 순으로 많았고, 과실 한개당 평균무게, 과장 및 과폭은 브라운터어키 품종이 가장 높았다. 우리나라 남부지역에서 시설내 양액재배에 가장 적당한 품종은 마쓰이도후인 품종과 브라운터어키 품종으로 추천되었다. This experiment was carried out to know the effect of cultivars and substrates on the growth and development of fig tree (Ficus carica L.) grown in hydroponics. Four cultivars ; Masui Dauphine, Bongraeshi, White Genoa and Brown Turkey, were used and four substrates, perlite, cocopeat, rockwool and granular rockwool were employed. Every cultivars cutted into 20cm with two buds on 27th, of February and then inserted into fockwool cube for root initiation, Every fifteen cuttings were transplanted on 17th of May in each substrate. Among cultivars 'Bongraeshi' showed highest growth in terms of plant height, number of leaves and shoots, and leaf length in the both rockwool and granular rockwool. Total yields per square meter and average yields per plant were higher in the order of Masui Dauphine > Brown Turkey > Bongraesh and White Genoa. Brown Turkey showed the heavier frjuit weight and larger fruit length and width than those of the other cultivars. Consequently, optimum cultivars and substradtes for hydroponics were recommended as Masui Dauphine and Brown Turkey, and rockwool and granular rockwool. However further trials would be followed as to the nutrient concentration, composition and solution managemint for improving fruit yield and quality of fig tree during growing period.

      • 암면재배 수박의 품종별 당함량 비교

        박순기,이범선,최진호,김월수,정순주 전남대학교 농업과학기술연구소 2000 農業科學技術硏究 Vol.35 No.-

        The fruit qualities and sugar contents of hydroponically grown watermelon cultivars were investigated in order to obtain informations for nutriculture of watermelon in glass house. The fruit circumference of Mudeungsan watermelon was increased from 30 to 38 days after pollination, and periods of fruit development was longer than that of other cultivars. Total soluble solid content in fruit flesh of watermelon was high in Samboggool, Gwigongja, Dalgona, Gamro, Mudeungsan, Olimpia, Hwangto and Geumro in order. Maltose content was higher in Gwigongja and Mudeungsan watermelon cultivar Sucrose content was higher in Samboggool, Gwigongja and Dalgona watermelon cultivar than that of other watermelon cultivars. Glucose content was much higher in Mudeungsan watermelon cultivar while very lower in Samboggool, Gwigongja and Dalgona watermelon cultivar than that of other watermelon cultivars. Fructose content was higher in Hwangto, Gamro, and Mudeungsan watermelon cultivar while was lower in Gwigongja watermelon cultivar than that of other watermelon cultivars. Maltose, glucose, and fructose content in fruit fresh of watermelon was higher in Mudeungsan watermelon than that of other watermelon cultivars. Contents of maltose, fructose and glucose in fruit flesh of watermelon were higher in Mudeungsan watermelon than those of other cultivars.

      • SCOPUSKCI등재

        술후 진통을 위해 투여한 Ketorolac이 혈액응고 기능에 미치는 영향 : 혈전탄성묘사도를 이용한 평가 A Thromboelastographic Evaluation

        최인범,고성훈,김동찬,한영진,최훈,민기철 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.6

        Background: Thromboelastography (TEG) has recently become popular for assessment of whole blood coagulation in the operating room. Ketorolac, a potent injectable nonsteroidal anti-inflammatory drug (NSAID), is commonly used for postoperative analgesia. NSAID inhibit plated aggregation in coagulation process. This study was designed to determine whether ketorolac used for postoperative analgesia can affect hemostatic function using a TEG. Methods: Seventy-four female patients, ASA physical status 1 or 2, scheduled for an elective gynecologic surgery were randomly allocated into one of four groups (Group 1: n=10, control without patient-controlled analgesia (PCA); Group 2: n=21, PCA with morphine 60 mg; Group 3: n=20, PCA with morphine 30 mg + ketorolac 90 mg; Group 4: n=23, PCA with ketorolac 180 mg). Blood samples were obtained for TEG analysis preoperatively and 24, 48 and 72 h after surgery. Cumulative drug dosage, visual analog pain scale, satisfaction degree and side effects were measured at 24, 48 and 72 h after surgery. Results: There were no significant differenced in TGE parameters among the four groups at each time. There were no significant differenced in visual analog pain scales and satisfaction degree among the three groups using PCA for postoperative analgesia. Among the three groups using PCA for postoperative analgesia, Group 2 experienced more side effects. Conclusions: Ketorolac does not affect hemostatic function for 3 days after surgery when administrated as a PCA drug. (Korean J Anesthesiol 2002; 43: 755~762)

      • 자궁내 태아 사망에 대한 임상적 고찰 (1990-1997)

        김희범,서기원 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

        Objective : Our purpose of this study was to evaluated the incidence, cause of intrauterine fetal death, termination method and the maternal complications of fetal death clinically. Patients : From January 1990 to December 1997, a retrospective study was made of the hospital records of 188 cases of the intrauterine fetal death among 42,576 deliveries after 20 weeks gestation by gestational age in Soonchunhyang Kumi Hospital. Results : The incidence of the fetal death in utero was 0.44%. The most common gestational week when intrauterine fetal death was detected was 35-40 gestational weeks(35.6%). The parity of mother in the fetal demise in utero was not different between nulliparous and multiparous women. The sex ratio of fetus in intrauterine death was not different between male and female. The cases of the fetal death in utero weighting less than 2500gm were 134 cases(71.2%). The causes of the intrauterine fetal death showed unknown causes(51.6%), maternal diabetes(11.2%), abruptio placenta(8.5%), congenital anomaly(8.0%), pregnancy induced hypertension(5.9%), intrauterine infection(5.3%), cord complication(4.3%), multiple causes(2.7%), vasa previa rupture(1.6%) and oligohydramnios(1.1%). The mode of the pregnancy termination for intrauterine fetal death showed prostaglandin E2(42.0%), oxytocin(34.5%), hysterotomy(11.2%), spontaneous delivery (9.6%), and misoprostol(2.7%). The maternal complication were hemorrhage(4.3%), infection(1.6%), cervical laceration(1.1%), and uterine rupture(0.5%). Conclusion : The cause in about 50% of the intrauterine fetal death could not be determined, so further studies must be made on mother, stillborn infants, placenta and umbilical card in cases where the causes in unknown for the purpose of good outcome and prevention of intrauterine fetal death in next pregnancy. And the studies investigating the safe and effective method for pregnancy termination are needed in order to reduced maternal complication and shortened termination time.

      • KCI등재

        진동에 의한 혈관 내 폐 보조장치의 산소전달 특성

        김기범,권대규,이삼철,김성종,정인수,오인혁,김기주,변윤섭,정경락 한국화학공학회 2004 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.42 No.2

        본 연구는 급성호흡부전환자를 치료하기 위하여 사용되고 있는 혈관 내 폐 보조장치의 기체전달을 향상시키기 위하여 중공사막에 진동기법을 사용하여 기체전달을 향상시키고자 시도하였다. 그리고 혈관 내 폐 보조장치를 정맥에 삽입하기 전, 혈관 내 폐 보조장치의 설계조건을 실험적 모델을 통하여 기체전달을 예측할 수 있는 예측식을 만들고자 하였다. 실험결과, 본 연구에서는 진동기법이 기체전달을 향상시키는데 효과적임을 확인할 수 있었다. 뿐만 아니라, 충진율과 가징 주파수의 함수관계를 이용하여 기체전달을 예측 할 수 있었다. 실험에 의하여 얻어진 결과는 예측식에 의하여 얻어진 결과와 유사한 결과를 얻었다. 그러므로 본 연구에서는 충진율과 가진 주파수의 함수를 이용하여 VIVLAD의 기체전달을 예측할 수 있었다. In this paper, we tried to improve gas exchange of the vibrating intravascular lung assist device(VIVLAD) using vibrating method in the hollow fiber membrane, for the patients suffering from acute respiratory distress syndrome(ARDS). And we tried to formularize prediction equations to make a prediction about gas transfer for designing intravenous artificial lung assist device, and designed modules under various conditions were studied through an experimental modeling before inserted the artificial lung assist device into as venous. As a result, we are convinced that vibration method is very useful for the has transfer increasing. Also, we can estimate the gas transfer as a function of the packing density and excited frequencies. The gas transfer obtained from the experiment was similar to that from the prediction equation, confirming the usefulness prediction equation. Therefore, we can estimate the gas transfer of the VIVLAD as a function of the packing density and excited frequencies.

      • 용기의 크기가 養液栽培 오이의 生長과 收量에 미치는 影響

        鄭淳柱,李範宣,朴順基,金光秀 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

        본 연구는 perlite를 이용한 오이의 양액재배시 적정 배지량과 근권용적을 구명하고자 용기의 크기가 2, 4, 6, 8 및 10ℓ인 플라스틱 포트에 펄라이트를 채워 오이를 양액재배하였다. 근권용적이 증가할수록 초장, 경경, 엽수 및 엽면적이 증가하였고, 과실수와 과실중량에서는 근권용적이 큰 8ℓ와 10ℓ의 처리구에서 가장 양호하였으며 기형과 발생률도 낮은 경향이었다. 근권용적 6ℓ이상으로 하였을 때 경으로의 건물분배가 줄어들고 엽으로의 건물분배가 많았다. NAR과 CGR은 근권용적이 증가함에 따라 증가하는 경향을 보였다. 근권용적에 관계없이 본 실험 범위내에서는 LAI가 증가할수록 NAR과 CGR도 증가하는 경향을 나타내었다. This experiment was carried out to investigate the effect of container size and substrate volume on the growth and fruit yield of hydroponically grown cucumber(Cucumis sativus L.) plant. Seeds were sown in plug tray filled with coir dust on Feb. 13, 1998. Seedlings with 5 to 6 true leaves were transplanted in 2l, 4l, 6l, 8l and 10l plastic pots filled with perlite medium. Cucumber fruits were harvested with 1 to 2 day interval, and fresh weight, number and malform of fruit were recorded. Plant height, stem diameter, number of leaves and leaf area highly depended on the container size. Total fresh weight and the number of fruit were increased as the container size increased. NAR(net assimilation rate) and CGR(crop growth rate) increased with increasing the container size. Optimum container size for hydroponically grown cucumber plant using perlite medium was recommended as 8l per plant.

      • 양액재배에서 배지종류가 멜론의 생장과 과실품질에 미치는 영향

        정순주,박순기,이범선,김월수 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

        배지종류가 멜론의 생육 및 과실품질에 미치는 영향을 구명하고자 코리아멜론을 이용하여 시설내에서 재배하여 실험한 바 다음과 같은 결과를 얻었다. 토경, 펄라이트경 및 토양격리상에 재배한 경우 생육전반기에는 초장, 엽면적, 각 기관별 생체중 및 건물중 등에서 펄라이트경과 토양격리상이 토경 멜론보다 좋았다. 토양, 왕겨 혼합배지 및 토양·펄라이트·피트모스 혼합배지(360ℓ:180ℓ:60ℓ)에 재배한 경우 생육초기에는 왕겨 혼합배지와 토양·펄라이트·피트모스 혼합배지에서 조사전체 항목에 걸쳐 생육이 양호하였다. 토경, 펄라이트경 및 토양격리상에 재배한 멜론의 과실품질을 보면 과중은 토경보다 처리구가 높았고 당도는 펄라이트경에서 13.3도로 가장 높았다. 산도는 펄라이트경에서 낮게 나타났다. 토양에 왕겨, 펄라이트 및 피트모스를 혼합하여 재배한 멜론의 과실품질를 보면 토양·왕겨 혼합배지에서 당도가 12.4도로 가장 높았고 과중은 토양에 펄라이트와 피트모스를 혼합한 경우가 높았다. The experiment was carried out to investigate the effects of substrates on the growth and fruit quality of the Korea melon (Cucumis melo L. var. reticulatus). Seedlings were grown in soil(not isolated) and polyethylene boxes (10mX50cmX12cm, vol. 600l) filled with perlite, SI (isolated soil culture), RHS [rice hull (200l)+soil (400l)], SP-1 (soil : perlite : peatmoss = 360l : 180l : 60l), SP-2 (soil : perlite : peatmoss = 300l : 225l : 75l), respectively. Plant height, leaf area, fresh and dry weights were higher in perlite culture and isolated soil culture than those of soil culture at the beginning of growth. The vegetative growth of melon in RHS and SP-1 were better than that of soil culture. Soluble solid content and fruit fresh weight were higher in perlite and SI culture than those of soil culture. In the case of mixing substrates, soluble solid content was higher in SP-1 than that of the other substrates. The isolated soil culture of mixing perlite, peat, and soil were recommended as substrate for hydroponically grown melon.

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