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- 저자 : Belyagoubi-Benhammou (검색결과 2 건)
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Belyagoubi Larbi,Belyagoubi-Benhammou Nabila,Atik-Bekkara Fawzia,Abdelouahid Djamel Eddine 경희대학교 융합한의과학연구소 2022 Oriental Pharmacy and Experimental Medicine Vol.22 No.2
Effects of extraction solvent and harvest period on phytochemical analysis and antioxidant and antimicrobial activities of extracts obtained from the freshwater macroalga, Spirogyra sp. were evaluated. The experiment lasted five months, where five crude extracts were obtained by maceration using different solvents: hexane, chloroform, dichloromethane, acetone and methanol. Higher values of yields were recorded in methanolic extracts on February (7.304%) and March (4.812%). All extracts were rich in proanthocyanidins compared to total phenolics and flavonoids. The total amounts of carotenoids were very low (0.060 ± 0001 to 0.171 ± 0.005 mg/g) compared to chlorophylls which were higher in February (6.365 ± 0.075 mg/g) and in March (5.501 ± 0.118 mg/g). The antioxidant activities indicated that the chloroformic and dichloromethanic extracts of February and March exhibited a good activity. The correlation using the Pearson test of phenolic compounds and antioxidant activity tests have confirmed the strong contribution of flavonoids and proanthocyanidins. The greatest antimicrobial activity was found in hexanic extracts at 0.1 to 1 mg/disk. The present findings suggest that Spirogyra sp. can be exploited not only for human health, but also for screening new economic natural antioxidants that could be used in foods, cosmetics and pharmaceutical industries.
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Nabila Belyagoubi-Benhammou,Larbi Belyagoubi,Nadjat Bechlaghem,Nacéra Ghembaza,Fawzia Atik-Bekkara 경희대학교 융합한의과학연구소 2017 Oriental Pharmacy and Experimental Medicine Vol.17 No.1
The antioxidant activity of the crude hydroalcoholic extract (PSMW) and its derived fractions n-hexane (PSH), diethylic ether (PSDE), chloroforme (PSC), ethyl acetate (PSEA), n-butanol (PSB) and residual aqueous fraction (PSA) of aerial part of endemic plant Pituranthos scoparius was performed using three in vitro tests : phosphomolybdate assay, reducing power and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging capacity. The total yield, total phenolic (TPC), flavonoid (TFC), flavonol (TF) and condensed tannin contents (TCT) of all the fractions were also determined. This plant was also subjected to preliminary phytochemical screening test for various constituents. Phytochemical screening revealed the presence of flavonoids, tannins, coumarins, saponins and steroids in the extracts. Amongst the extracts, PCMW showed the highest TPC (8.40 ± 0.01 mg gallic acid equivalent/g of dry matter), TFC (2.25 ± 0.01 mg catechin equivalent/g), TF (1.19 ± 0.04 mg quercetin equivalent/g) and TCT (1.07 ± 0.16 mg catechin equivalent/g). The Total Antioxidant Capacity (CAT) was found to be varied in different fractions from 0.11 ± 0.00 to 10.02 ± 0.02 mg ascorbic acid equivalent/g). Therefore, PSEA showed the highest antioxidant activities to reduce iron (IC50 = 1.19 ± 0.00 mg/mL) and to scavenge DPPH (IC50 = 0.91 ± 0.02 mg/mL).
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