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Francisco Claudio Dantas Mota,Duvaldo Eurides,Patricia Maria Coletto Freitas,Marcelo Emilio Beletti,Michelle Rodriques Goulart,Livia Maria Ferreira Cunha,Luiz Antonio Franco da Silva,Maria Clorinda So 대한수의학회 2004 Journal of Veterinary Science Vol.5 No.3
The aim of this study was to evaluate the cicatricial repair of perforating cornea in rabbits, by using the Nbutyl cyanoacrylate adhesive compared to the 910- polyglactine thread suture through macroscopic and histological assays. Corneas from 18 adult rabbits were perforated and subsequently occluded with N-butyl cyanoacrylate synthetic adhesive (right cornea) or by separated single points using the 910-polyglactine thread (left cornea). The rabbits were divided into groups containing three animals per group. Examination after 7, 15, and 30 days post-operative showed that both the synthetic adhesive and the suture were efficient in the occlusion of the surgical wounds, thus stabilizing the intra-ocular content. The N-butyl cyanoacrylate adhesive was shown to be superior to the 910-polyglactine suture thread with regards to the evolution and the organization of the healing process.
Rosiane Nascimento Alves,Susana Elisa Rieck,Carlos Ueira-Vieira,Marcelo Bahia Labruna,Marcelo Emílio Beletti 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.2
Amplification of the 16S rRNA gene from a blood sampleobtained from a dog in southeastern Brazil was used to confirma naturally acquired Ehrlichia (E.) canis infection. Followingisolation and culturing of the new bacterial strain calledUberlândia, partial sequences of the dsb and p28 genes wereobtained. The dsb partial sequence of the novel strain was100% similar to dsb gene sequences of E. canis obtained fromdifferent geographic areas around the world. Conversely, thep28 partial sequence for the E. canis Uberlândia strain differedat several nucleotides from other sequences available inGenBank. To confirm the antigenic profile of the Uberlândiastrain, an indirect immunofluorescence assay against E. canisantigens was performed using dog sera collected from twodifferent areas in Brazil (Uberlândia and São Paulo). Theresults suggest that both antigens were able to identify animalsseropositive for E. canis in Brazil since these Brazilian strainsappear to be highly conserved.
Letícia Z. Oliveira,Vera F. M. Hossepian de Lima,Marcelo A. Levenhagen,Ricarda M. dos Santos,Terezinha I. Assumpção,José O. Jacomini,André F. C. de Andrade,Rubens P. de Arruda,Marcelo E. Beletti 대한수의학회 2011 Journal of Veterinary Science Vol.12 No.3
The objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation of cryopreserved bovine sperm. Semen was collected from six bulls of different breeds and three ejaculates per bull were evaluated. Frozen semen samples were thawed and the acrosomal region of sperm cells was evaluated by transmission electron microscopy (TEM) before (n = 18) and after (n = 18) Percoll centrifugation. The evaluation of 20 sperm heads from each of the 36 samples analyzed ensured that a large number of cells were investigated. The data were subjected to analysis of variance at a level of significance of 5%. Percoll centrifugation reduced the percentage of sperm exhibiting normal acrosomes (from 61.77 to 30.24%), reduced the percentage of sperm presenting atypical acrosome reactions (from 28.38 to 4.84%) and increased the percentage of sperm exhibiting damage in the acrosome (from 6.14 to 64.26%). The percentage of sperm with typical acrosome reactions was not significantly different before (3.70%) and after (0.67%) centrifugation. TEM distinguished four different types of acrosomal status and enabled ultrastructural characterization of acrosomal injuries. The percentage of sperm exhibiting normal acrosomes decreased and damage in the acrosome was the most frequent acrosomal injury with the Percoll gradient centrifugation protocol utilized.