Glucose Metabolism Influences Cytoplasmic Maturation in Porcine Oocytes

Bao Yuan,Shuang Liang,Jiabao Zhang,Jeong-Woo Kwon,Shun-Ha Park,Hai-Yang Wang,PIAO XUAN JING,Xiang-Shun Cui,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2016 발생공학 국제심포지엄 및 학술대회 Vol.2016 No.10

Glucose Metabolism Influences Cytoplasmic Maturation in Porcine Oocytes

Bao Yuan,Shuang Liang,Jiabao Zhang,Jeong-Woo Kwon,Shun-Ha Park,Hai-Yang Wang,PIAO XUAN JING,Xiang-Shun Cui,Nam-Hyung Kim 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10

In the present study, we examined potential roles of glucose and pyruvate in nuclear and cytoplasmic maturation of porcine oocytes. In the presence and absence of 10% porcine follicular fluid (PFF), either 5.6 mM glucose or 2mM pyruvate effect on meiotic maturation and followed development ability. However, DOs doesn't take full advantage of the glucose in medium, only pyruvate can increase MII rate and follow early embryo development ability significance. COCs were matured with 200 uM pentose phosphate pathway (PPP) inhibitor (dehydroepiandrosterone, DHEA) or 2 μM glycolysis inhibitor (iodoacetate, IA), significantly lower levels of GHS in the DHEA an IA treated oocytes and the levels of ROS were higher significantly in the DHEA treated oocytes, treatment with DHEA significantly reduced the intra-oocyte ATP and NADPH level. Blastocysts from DHEA or IA treated group also presented higher apoptosis levels, meanwhile, the percentage of proliferating cells was dramatically lower than the non-treated group. In conclusion, our results suggest that 10% PFF promoted oocytes make full use of energy, glucose metabolism during in vitro maturation inseparable from the cumulus cells, PPP and glycolysis promoted porcine oocytes cytoplasmic maturation by supplying energy and reducing oxidative stress.

A Secure Multi-receivers E-mail Protocol

Baoyuan Kang,Danhui Xu 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.11

In open decentralized networks, it is important to make certain data available to only a selected group of users. For example, in a secure e-mail system, a user may send an e-mail to multiple receivers at once. Recently, Chen proposed a secure multicast key protocol for e-mail system based on Chinese Remainder Theorem. They claimed that their protocol provide perfect forward secrecy and ensure confidentiality and authentication. But, in this paper, we show that Chen’s protocol suffers from the sender and the e-mail server impersonation attacks and mail content confidentiality attack. Moreover, we give an improvement to Chen’s protocol. To contribute a secure multireceiver e-mail protocol, we propose a novel protocol by adopting Lagrange polynomial interpolation. We also discuss the security of the novel multireceiver e-mail protocol. Our protocol provides the perfect forward secrecy and resists unknown key-share attack, replay attack, sender impersonation attack, e-mail server impersonation attack and mail content confidentiality attack.

An Untraceable Off-Line Electronic Cash Scheme without Merchant Frauds

Baoyuan Kang,Danhui Xu 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.1

In an electronic cash scheme, there are three participants, the bank, the spender and the merchant. First, a spender opens an account in a bank. Then, he withdraws electronic cash from his account and pays it to a merchant. After checking the electronic cash’s validity, the merchant accepts it and deposits it to the bank. There are a number of requirements for an electronic cash scheme, such as, anonymity, unforgeability, unreusability, date attachability, divisibility, transferability and portability. In this paper, we show a recently proposed electronic cash scheme is suffering from some faults in anonymity, expiration date and merchant frauds. To improve the scheme, we propose a new untraceable off-line electronic cash scheme and give a security analysis for it.

A secure Certificateless Aggregate Signature Scheme

Baoyuan Kang,Danhui Xu 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.3

Aggregate signatures allow n signatures on n distinct messages from n distinct signers to be aggregated into a single signature that convinces any verifier that n signers do indeed sign the n messages, respectively. The major advantage of utilizing aggregate signatures is to address the security of data and save bandwidth and computations in sensor networks. Recently, people discuss aggregate signature in certificateless public key setting. But some existing certificateless aggregate signature schemes are not secure. In this paper, we analyze the security of Zhang et al.’s certificateless aggregate signature schemes, and propose a new ew ew certificateless aggregate signature schemes, and prove the new scheme is existentially unforgeable under adaptive chosen-message attacks under the assumption that computational Diffie–Hellman problem is hard. Furthermore, in signing equation of the proposed scheme user’s partial private key and secret value are directly combined with the signed message. So, the scheme is also secure against some inside forgery attack.

α-MSH suppresses neuroinflammation and improves neurobehavioral outcomes after traumatic brain injury in mice

Jin Baoyuan,Kim Hyehyun,Jeong Seongtae 조선대학교 의학연구원 2023 Medical Bilogical Science and Engineering Vol.6 No.1

Traumatic brain injury (TBI) leads to a cascade of neuroinflammation and subsequent long-term cognitive deficits. Alpha melanocyte stimulating hormone (a-MSH) is a neuropeptide that protects against TBI. In this study, we aimed to evaluate the effect of a-MSH on TBI induced brain inflammation. a-MSH improved rotarod latencies during the first 3 days and water maze latencies over 29-32 days. Here, a-MSH-treated mice had significantly lower tumor necrosis factor-alpha (TNF-a) concentrations in the cortex at 30 min and 1 h. The mitogen-activated protein kinase (MAPK) isoforms JNK, ERK, and p38 decreased following administration of a-MSH. The inhibitor of nuclear factor-kB (IkB) kinase (IKK)/Nuclear factor-kB (NFkB) signaling system is a key regulator of inflammation. Phosphorylation of IKK/NFkB increased after TBI but decreased significantly in response to a-MSH. Strongly immunoreactive microglia increased and were observed throughout the hippocampus in the TBI model, whereas a-MSH-treated mice showed less activation. TNF-a concentrations tended to decrease in the hippocampus. These data indicate that a-MSH might attenuate inflammation in a TBI mouse model.

BRAF-Activated Long Noncoding RNA Modulates Papillary Thyroid Carcinoma Cell Proliferation through Regulating Thyroid Stimulating Hormone Receptor

Haitao Zheng,Meng Wang,Lixin Jiang,Haidi Chu,Jinchen Hu,Jinyao Ning,Baoyuan Li,Dong Wang,Jie Xu 대한암학회 2016 Cancer Research and Treatment Vol.48 No.2

Purpose The importance of long noncoding RNAs (lncRNAs) in tumorigenesis has recently been demonstrated. However, the role of lncRNAs in development of thyroid cancer remains largely unknown. Materials and Methods Using quantitative reverse transcription polymerase chain reaction, expression of three lncRNAs, including BRAF-activated long noncoding RNA (BANCR), papillary thyroid cancer susceptibility candidate 3 (PTCSC3), and noncoding RNA associated with mitogen-activated protein kinase pathway and growth arrest (NAMA), was investigated in the current study. Results Of the three lncRNAs (BANCR, PTCSC3, and NAMA), expression of BANCR was significantly up-regulated while PTCSC3 and NAMA were significantly down-regulated in papillary thyroid carcinoma (PTC) compared to that in normal tissue. BANCR-knockdown in a PTC-derived cell line (IHH-4) resulted in significant suppression of thyroid stimulating hormone receptor (TSHR). BANCR-knockdown also led to inhibition of cell growth and cell cycle arrest at G0/G1 phase through down-regulation of cyclin D1. In addition, BANCR was enriched by polycomb enhancer of zeste homolog 2 (EZH2), and silencing BANCR led to decreased chromatin recruitment of EZH2, which resulted significantly reduced expression of TSHR. Conclusion These findings indicate that BANCR may contribute to the tumorigenesis of PTC through regulation of cyclin D1 and TSHR.

Pharmacological blockade of cholesterol trafficking by cepharanthine in endothelial cells suppresses angiogenesis and tumor growth

Lyu, Junfang,Yang, Eun Ju,Head, Sarah A.,Ai, Nana,Zhang, Baoyuan,Wu, Changjie,Li, Ruo-Jing,Liu, Yifan,Yang, Chen,Dang, Yongjun,Kwon, Ho Jeong,Ge, Wei,Liu, Jun O.,Shim, Joong Sup Elsevier 2017 Cancer letters Vol.409 No.-

<P><B>Abstract</B></P> <P>Cholesterol is an important modulator of membrane protein function and signaling in endothelial cells, thus making it an emerging target for anti-angiogenic agents. In this study, we employed a phenotypic screen that detects intracellular cholesterol distribution in endothelial cells (HUVEC) and identified 13 existing drugs as cholesterol trafficking inhibitors. Cepharanthine, an approved drug for anti-inflammatory and cancer management use, was amongst the candidates, which was selected for in-depth mechanistic studies to link cholesterol trafficking and angiogenesis. Cepharanthine inhibited the endolysosomal trafficking of free-cholesterol and low-density lipoprotein in HUVEC by binding to Niemann-Pick disease, type C1 (NPC1) protein and increasing the lysosomal pH. The blockade of cholesterol trafficking led to a cholesterol-dependent dissociation of mTOR from the lysosomes and inhibition of its downstream signaling. Cepharanthine inhibited angiogenesis in HUVEC and in zebrafish in a cholesterol-dependent manner. Furthermore, cepharanthine suppressed tumor growth in vivo by inhibiting angiogenesis and it enhanced the antitumor activity of the standard chemotherapy cisplatin in lung and breast cancer xenografts in mice. Altogether, these results strongly support the idea that cholesterol trafficking is a viable drug target for anti-angiogenesis and that the inhibitors identified among existing drugs, such as cepharanthine, could be potential anti-angiogenic and antitumor agents.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A phenotypic screen identified 13 existing drugs, including cepharanthine, as cholesterol trafficking inhibitors. </LI> <LI> Cepharanthine inhibited lysosomal cholesterol trafficking by binding to NPC1 protein and increasing the lysosomal pH. </LI> <LI> The blockade of cholesterol trafficking led to a cholesterol-dependent dissociation of mTOR from the lysosomes. </LI> <LI> Cepharanthine inhibited angiogenesis in HUVEC and in zebrafish in a cholesterol-dependent manner. </LI> <LI> Cepharanthine treatment enhanced the antitumor activity of cisplatin in lung and breast cancer xenografts in mice. </LI> </UL> </P>