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Bao‑Jian Zhu,Lin Tang,Ying‑Ying Yu,Dao‑Jun Wang,Chao‑Liang Liu 한국유전학회 2017 Genes & Genomics Vol.39 No.6
Ecdysteroids play an important role in the regulation of molting process in crustaceans. The suppression subtractive hybridization (SSH) technique was employed to search for 20-hydroxyecdysone-responsive genes in the hepatopancreas of the crayfish Procambarus clarkii. Ninety-three putative expressed sequence tags were identified by SSH, including three immune response-related genes, two cell cycle and apoptosis genes, four respiration and energy metabolism genes, four transport-related genes, six metabolism-related genes, two stress response genes, and eight transcription and translation regulation genes. The expression levels of the examined genes were regulated by 20-hydroxyecdysone and varied in the hepatopancreas during the molting stages; data were confirmed by real-time PCR. RNAi of the ecdysteroid receptor had significant effects on the expression levels of 20-hydroxyecdysone- responsive genes. These differentially expressed genes identified by SSH will provide insight into the ecdysteroid signaling pathway in P. clarkii.
Extensive reprogramming of cytosine methylation in Oryza allotetraploids
Ying Bao,Qing Xu 한국유전학회 2015 Genes & Genomics Vol.37 No.6
Cytosine methylation is a prominent epigenetic modification in plants and it has been proposed to as causative for silencing some duplicates derived by polyploidization. Several synthetic polyploids have shown there is a dynamic epigenetic change following hybridization and genome doubling. Relatively few studies, however, have focused on wild natural polyploids. In this study, using a methylation-sensitive amplified fragment length polymorphism method, we investigated the levels and types of cytosine methylation in three Oryza CCDD genome allotetraploids and their related diploids with CC and EE genomes. We show an overall minor increase in methylation level in the allopolyploids, but extensive methylation reprogramming (70 %), with almost equivalently increased (22–26 %) and decreased methylation (25–27 %). Our study reveals a complicated epigenetic change in natural polyploids. The information presented in the study will be of some utility to molecular taxonomists and also rice breeders working on wide-hybridization.
( Ying Hua Bao ) 전북대학교 인문학연구소 2013 건지인문학 Vol.10 No.-
Based on the previous research and the investigative data avail-able in the pasture, the paper expounds that the marriage and fam-ily of the Ewenki ethnic group are tending to the sense of national identity, and delineates that the distinction of the Ewenki ethnic group is gradually confused because of the overwhelming trends of modernization, in addition, socialization has changed the mode of ethnic group identity obtained through marriage and family relations.
중국 호텔 인터넷 이용자의 재구매 의도에 미치는 인과관계
포형(Ying Bao),김영태(Young Tae Kim),최현정(Hyun Jung Choi) 한국관광연구학회 2012 관광연구저널 Vol.26 No.5
본 연구의 목적은 호텔 홈페이지를 이용한 경험이 있는 중국인 고객을 대상으로 인터넷 서비스품질, 인터넷 고객만족, 고객 신뢰, 그리고 재구매 의도의 구조적인 관계를 분석하고, 이에 따른 효과적인 대책을 논하는 것이다. 실증적인 분석을 위한 표본추출을 위하여 중국의 북부와 남부, 그리고 중심부에 각각 위치한 대표적인 도시와 호텔을 선정하여 약2주 동안 호텔 고객을 대상으로 설문조사를 실시하였고, 이에 따라 362개의 유효 표본이 본 분석에 활용되었다. 본 연구의 자료 분석을 위하여 SPSS(12.0)과 AMOS(5.0)이 활용하였으며, 빈도분석, 신뢰도 분석, 확인적 요인 분석 그리고 구조적 모형 분석이 진행되었다. 분석 결과, 인터넷 서비스품질의 5개 요인 중 4개 요인(정보품질, 고객개인화, 편리성, 반응성)이 인터넷 고객만족에 유의한 영향을 미치는 것으로 나타났다. 또한 인터넷 고객만족은 고객 신뢰와 재구매 의도에 유의한 영향을 미치는 것으로 나타났으며, 고객 신뢰는 재구매 의도에 긍정적인 영향을 미치는 것으로 확인되었다.
Memory Enhancing and Neuroprotective Effects of Selected Ginsenosides
Hai Ying Bao,Jing Zhang,여수정,Chang-Seon Myung,Hyang Mi Kim,Jong Moon Kim,박정일,조정숙,Jong Seong Kang 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.3
The effects of ginsenosides Rg3(R), Rg3(S) and Rg5/Rk1 (a mixture of Rg5 and Rk1, 1:1, w/w), which are components isolated from processed Panax ginseng C.A. Meyer (Araliaceae), on memory dysfunction were examined in mice using a passive avoidance test. The ginsenosides Rg3(R), Rg3(S) or Rg5/Rk1, when orally administered for 4 days, significantly ameliorated the memory impairment induced by the single oral administration of ethanol. The memory impairment induced by the intraperitoneal injection of scopolamine was also significantly recovered by ginsenosides Rg3(S) and Rg5/Rk1. Among the three ginsenosides tested in this study, Rg5/Rk1 enhanced the memory function of mice most effectively in both the ethanoland scopolamine-induced amnesia models. Moreover, the latency period of the Rg5/Rk1-treated mice was 1.2 times longer than that of the control (no amnesia) group in both models, implying that Rg5/Rk1 may also exert beneficial effects in the normal brain. We also evaluated the effects of these ginsenosides on the excitotoxic and oxidative stress-induced neuronal cell damage in primary cultured rat cortical cells. The excitotoxicity induced by glutamate or Nmethyl- D-aspartate (NMDA) was dramatically inhibited by the three ginsenosides. Rg3(S) and Rg5/Rk1 exhibited a more potent inhibition of excitotoxicity than did Rg3(R). In contrast, these ginsenosides were all ineffective against the H2O2- or xanthine/xanthine oxidase-induced oxidative neuronal damage. Taken together, these results indicate that ginsenosides Rg3(S) and Rg5/Rk1 significantly reversed the memory dysfunction induced by ethanol or scopolamine, and their neuroprotective actions against excitotoxicity may be attributed to their memory enhancing effects.
Bao-Huan Yang,Chia-Ying Chung,Yuh-Shiow Li 한국간호과학회 2018 Asian Nursing Research Vol.12 No.2
Purpose: At present, there is still controversy between parents of children with muscular dystrophy (MD) and health-care professionals on care issues. Partnerships can connect the affected children and their families to appropriate health-care services, to jointly face the care environment together and thereby improve the quality of life of children with MD. Therefore, the objective of this study is to explore partnerships between families and health-care professionals from the perspectives of parents of children with MD. Methods: Husserl's phenomenological research was applied to explore the basic structures of parents' descriptions of MD. Through purposive sampling, we conducted in-depth interviews with parents, and analyzed the data according to the theory of Giorgi. Nineteen parents (10 mothers, 9 fathers) participated in this study. The precision of the research results was tested by applying the four standards of Lincoln and Guba. Results: This study identified five constituents: feasible resources and detailed care information; the provision of an integrated medical care across systems; family and home as key elements in critical care; respect and care for family care demands; and finally, feedback and support from families. Conclusion: This study demonstrated that partnerships were established by health-care professionals, enhancing the care capacity of the families, developing the preventive medicine of MD, and enhancing children's potential for self-care within the families. Hospital policies should include the promotion of family partnership care. The findings can help health-care professionals recognize the life experiences of children with MD when providing medical care.
Effect of fangchinoline on root resorption during rat orthodontic tooth movement
Bao, Xingfu,Hu, Min,Zhang, Yi,Machibya, Ferdinand,Zhang, Ying,Jiang, Huan,Yu, Dongsheng The Korean Association Of Orthodontists 2012 대한치과교정학회지 Vol.42 No.3
Objective: To evaluate the short-term effect of fangchinoline, an anti-inflammatory drug widely used in Asia, on root resorption that is associated with orthodontic tooth movement. Methods: Twenty-four Wistar rats were randomly divided into 6 groups. Mesial forces of 0, 50, or 100 g were applied to the maxillary first molar of the rats in each group for 14 days by activating nickel-titanium closed-coil springs. One-half of the rats receiving each of these treatments also received injections of 200 ${\mu}L$ fangchinoline every 2 days. Finally, movement of the maxillary first molars was measured using digitized radiographs. The molars were extracted and the surfaces of the root resorption craters were recorded using a scanning electron microscope. The distance the molars moved and resorption-area ratio was measured, and results were analyzed using 2-way ANOVA tests. Results: There were no statistical differences in the distances the first molars moved under 50 or 100 g force, regardless of treatment with fangchinoline. However, the resorption area ratios were significantly smaller in those rats that were treated with both tension and fangchinoline than in those rats treated by tension alone. Conclusions: Fangchinoline reduced the resorption area ratio in rats and is therefore an important means of alleviating root resorption.