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Bang, Ye-Ji,Lee, Zee-Won,Kim, Dukyun,Jo, Inseong,Ha, Nam-Chul,Choi, Sang Ho American Society for Biochemistry and Molecular Bi 2016 The Journal of biological chemistry Vol.291 No.31
<P>The bacterial transcriptional regulator OxyR is known to function as a two-state redox switch. OxyR senses cellular levels of H2O2 via a 'sensing cysteine' that switches from the reduced to a disulfide state upon H2O2 exposure, inducing the expression of antioxidant genes. The reduced and disulfide states of OxyR, respectively, bind to extended and compact regions of DNA, where the reduced state blocks and the oxidized state allows transcription and further induces target gene expression by interacting with RNA polymerase. Vibrio vulnificus OxyR2 senses H2O2 with high sensitivity and induces the gene encoding the antioxidant Prx2. In this study, we used mass spectrometry to identify a third redox state of OxyR2, in which the sensing cysteine was overoxidized to S-sulfonated cysteine (Cys-SO3H) by high H2O2 in vitro and in vivo, where the modification deterred the transcription of prx2. The DNA binding preferences of OxyR2(5CA)-C206D, which mimics overoxidized OxyR2, suggested that overoxidized OxyR2 binds to the extended DNA site, masking the -35 region of the prx2 promoter. These combined results demonstrate that OxyR2 functions as a three-state redox switch to tightly regulate the expression of prx2, preventing futile production of Prx2 in cells exposed to high levels of H2O2 sufficient to inactivate Prx2. We further provide evidence that another OxyR homolog, OxyR1, displays similar three-state behavior, inviting further exploration of this phenomenon as a potentially general regulatory mechanism.</P>
정신지체 고등학생을 위한 의사결정기술 교수프로그램의 적용효과
방명애(Bang, Myong-Ye),김수현(Kim, Soo-Hyun) 한국언어청각임상학회 2001 Communication Sciences and Disorders Vol.6 No.2
본 연구는 정신지체 고등학생에게 의사결정기술 교수프로그램을 적용하여 효과를 검증하는 것을 목적으로 하였다. 연구대상은 특수학교 고등부 정신지체 여학생 9명이었으며, 2001년 1학기에 41회기에 걸쳐 의사결정기술 프로그램을 적용하였는데, 담임교사와 부모가 프로그램을 실시하기 전후에 각각 아동의 의사결정기술을 평가하였다. 수집된 자료를 대응표본 t- 검정과 상관계수를 통해 분석한 결과는 다음과 같다. 첫째, 교사로부터 수집된 사전ㆍ사후검사 자료를 대응표본 t- 검정을 적용하여 분석한 결과, 사전검사의 평균과 사후검사의 평균간에 통계적으로 유의미한 차이가 있었다. 즉, 교사는 연구대상인 정신지체 고등학생의 의사결정기술이 프로그램을 적용한 후 향상되었다고 인식하였다. 둘째, 부모로부터 수집된 사전ㆍ사후검사 자료를 대응표본 t- 검정을 적용하여 분석한 결과, 사전검사의 평균과 사후검사의 평균간에 통계적으로 유의미한 차이가 없었다. 부모로부터 수집된 자료에 따르면 사후검사의 평균이 사전검사의 평균에 비해 향 상되었으나, 통계적으로 유의미한 차이는 아니었다. 추후연구를 통해 각 장애영역에 있어서의 의사결정기술에 대한 기초연구와 교수프로그램의 효과를 검증해야겠다. The purpose of this study was to investigate the effects of a self- determination program on the self- determination skills of high school student s with mental retardation. One- group pre- test -post - test research design was used for this study. Nine high school girls with mental retardation participated in the self- determination program. Self- determination skills of subject s were measured by their teacher s and parents before and after implementing the program using Self-Determination Teacher Perception Scale and Self-Determination Parent Perception Scale developed by Hoffman, Field, and Sawilosky (2000). Matched pair t- test was used for the analyses. The research findings were as follows: (1) According to the teacher perception, there was a statistically significant improvement of self- determination skills of high school students with mental retardation after implementing the self- determination program. (2) According to the parent perception, there was no statistically significant improvement of self- determination skills of high school student s with mental retardation even after implementing the self- determination program. The implications of the research results are discussed.