論文(론문) : 大腸正格證(대장정격증)과 장누수증후군(Leaky Gut Syndrome)과의 관계 考察(고찰)

이진철 ( Jin Cheoul Lee ),박상균 ( Sang Kyun Park ),방정균 ( Jung Kyun Bang ) 대한한의학원전학회(구 대한원전의사학회) 2013 대한한의학원전학회지 Vol.26 No.4

A Study on the Relationship between Dae-Jang-Jung-Gyeok(大腸正格) and Leaky Gut Syndrome Lee Jin-Cheoul1·Park Sang-Kyun2·Bang Jung-kyun3 *1Dept. of Medical History, College of Korean Medicine, Kyunghee University 2Dept. of Meridian, College of Korean Medicine, Sangji University 3Dept. of Korean Medical Classics and History, College of Korean Medicine, Sangji University Objective: If the penetration ratio of the intestinal mucosa is increased, the toxic or unhealthy materials - which should not be absorbed into our bodies - will be come into our bodies. They cause a sort of anti-toxic response or confusion of the immune system, and ultimately bring various types of diseases. This syndrome is related "Poison in Excrement" or "Internal Damage(內傷發□)" and Leaky Gut Syndrome, so I will study on the relationship between them. Method: Study on the relationship between Dae-Jang-Jung-gyeok and Leaky Gut Syndrome. Result: Korean doctors in ancient times called this syndrome "Poison in Excrement" or "Internal Damage(內傷發□)" and treated it with "Dae-Jang-Jung-Gyeok(大腸正格)". Leaky Gut Syndrome is a sort of clinical lesion, which allows foreign and harmful toxins in and results in a disorder of the immune system due to the leaking intestinal mucosa. Conclusion: Based upon the analyses, Dae-Jang-Jung-Gyeok and Leaky Gut Syndrome are closely related. Therefore diseases caused by the Leaky Gut Syndrome might be expected to be cured by Dae-Jang-Jung-Gyeok. Particularly the use of treatments for self-immune diseases and allergic diseases such as atopyic dermatitis and articular rheumatism should be expected to reveal a new path of treatment for other disorders such as Leaky Gut Syndrome. Key Words: Poison in Excrement, Internal Damage(內傷發□), Dae-Jang-Jung-Gyeok(大腸正格), Leaky Gut Syndrome

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

타액분비량이 증가하는 입체조의 효과와 만족도

방혜인(외 3명) 신라대학교 자연과학연구소 2012 自然科學論文集 Vol.22 No.-

Objectives: This study was performed to grasp the relation between the effect and the satisfaction of mouth gymnastics program on the promotion of oral function of students. We investigate the degree of digestion due to stress and the changes in salivary flow. We should investigate about the relationship of xerostomia according to the changes in salivary secretion. Methods: Total 79 students for two weeks, Silla University Dental Hygiene Department participated in this survey. Results: In the point of view of oral xerostama, people without drinking have lower symptom and action of xerpstama than those who drink three or four times a week. As the change rate of salivation amount is large, the degree of digestion, stress and xerpstama symptom and action is low. However the satisfication of oral exercise program is high. Conclusions: Adult should pay attention to oral health through oral exercise which increases the amount of salivation. Also Oral and total health should be increased through oral health education for the methodical oral health program

추간판 탈출의 기질 흡수에 관한 혈관 신생과 대식구의 역할

양진서,허방 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

Purposes: This study is performed to establish the histologic characteristics of matrix resorption in herniated intervertebral disc and to evaluate the role of neovascularization and macrophage infiltration, in especially concerning symptom duration and radiologic type of disc herniation. Materials & methods: Total seventy-two cases of herniated disc including protrusion 26, extrusion 24, and sequestration 22 cases were selected. The scales of matrix resorption, and vascularization were devided into 4 groups according to the extent and grade of each histologic changes. Through immunohistochemical staining (LSAB method) with monoclonal antibody CD34 (Neomarker, USA) and CD68 (DAKO, Denmark) in serial sections of paraffin embedded tissues, the expression of CD34 and CD68 in herniated discs was evaluated. Results: The incidence of matrix resorption and cell degeneration was 54.2%, which was markedly increased in prolonged (more than 1 month) symptom duration group and protruded or sequestrated type. Neovascularization, highlighted with CD 34 expression tends to be random and marginal, CD68 expressed macrophages were noted in well-vascularized region. Microvessel density (MVD) calculated by CD34 expression and CD68 expression in cases of matrix resorption were also pronounced in prolonged symptom duration group and protruded or sequestrated type. Conclusion: The authors confirm an important role of angiogenesis and macrophages in matrix resorption of disc herniation, especially in clinically prolonged and more severely herniated cases.

복사열에 노출된 작업복의 열적특성에 관한 실험적 연구

방창훈,이진호,예용택 한국산업안전학회 2002 한국안전학회지 Vol.17 No.3

The purpose of this study was to present the thermal characteristics of the working uniform exposed to the radiation heat. The effect of exposure time and exposure distance on the changes and the relationship between physical properties were investigated experimentally. Regardless of the kind of working uniform, the surface temperature of the working uniform with exposed time sharply increases as exposed distance is more close and the reaching tie of steady state is shorter. The surface temperature of working uniform exponentially decreases as exposed distance become more distant. For the safety of the working man, it is necessary that he work far away at a fixed standard distance from the radiant heat source.

표면 거칠기에 따른 액적의 증발 냉각

방창훈,권진순,예용택 한국안전학회 2003 한국안전학회지 Vol.18 No.3

The objective of the present work is to examine evaporation cooling of droplet due to surface roughness on a heated surface. The surface temperatures varied from 80~160℃ on aluminum alloy (AL 2024) and surface roughness was 0.18μm, 1.36μm. The results are as follows; Regardless of surface roughness, as droplet diameter is bigger, the in-depth temperature of solid decreases and evaporation time increases. In the case of same initial temperature on the heated surface, as droplet diameter is smaller and small surface roughness is bigger, evaporation time decreases and time averaged heat flux increases.

질분만에 따른 음부신경 손상 및 그 회복 양상

이상전,박진우 대한대장항문학회 1997 Annals of Coloproctolgy Vol.13 No.1

복사가열조건에서 표면 거칠기에 따른 액적의 증발 냉각

방창훈,권진순,예용택 한국안전학회 2004 한국안전학회지 Vol.19 No.3

The objective of the present work is to examine evaporation cooling of droplet due to surface roughness under radiative heat input condition. The surface temperatures varied from 80~160℃ on aluminum alloy (AL 2024) and surface roughness was 0.18μm, 1.36μm. The results are as follows; Regardless of surface roughness under radiative heat input condition, as droplet diameter is larger, the in-depth temperature of solid decreases and evaporation time increases. In the case of 0.18μm and 1.36μm of surface roughness, the larger the surface roughness is, the less the evaporation time is and the larger the temperature within the solid is. In the case of Ra = 0.18μm, evaporation time and time averaged heat flux for radiative heat input case is shorter than for the conductive case.

방사선 BY-445가 생성하는 α-amylase 저해물질의 특성

방병호,이진영 서울보건대학 1997 서울보건대학 부설 한국보건과학연구소 논문집 Vol.4 No.1

α-amylase에 대한 강력한 저해물질을 생산하는 방사균 BY-445를 토양으로부터 분리하였으며 이 균이 생산하는 배양액을 Amberlite IRA 420 column, Silica gel column chromatography 및 Sephadex G-50 column을 이용하여 조정제하였다. 이때 수율은 21.4%였으며 비활성도는 6배 증가하였다. 그리고 저해제의 α-amylase 100㎍에 대해 2배의 농도인 200㎍에서 저해활성이 거의 95%로 최고에 달하였다. 본 저해물질의 활성에 미치는 금속이온의 영향으로 Co^(+2), Hg^(+2), Pb^(+2), 및 Cu^(+2) 등에 의해 상대활성이 각각 13, 30, 37 및 72%로 각각 나타났다. 또한 본 저해물질은 열에 대한 안정성이 커서 100℃에서 120분간의 열처리를 하여도 그 활성은 거의 잔존하였으며 또한 전 pH 범위(pH2.0~12.0)에서도 아주 안정하였다. 본 저해물질의 여러가지 생화학적 반응을 검토한 결과 Anthrone, Molisch, Phenol-sulfuric acid반응에서는 양성을 띠었으며, Ninhydrin, Biuret, Benedict 등의 반응에서는 음성으로 나타났으며, 각종 carbohydrases에 대한 본 저해물질의 활성을 검토한 결과 사람 침 α-amylase와 Bacillus subtilis,porcine pancreas,Asp.niger의 glucoamylase등의 α-amylase에 대한 저해율이 각각 87%와 81,79,63%로 나타났으며, Aspergillus속의 cellulase와 Saccharomyces cerevisiae의 invertase에 대한 저해는 나타나지 않았다. A strain of Actinomycetes BY-445 isolated from soil was able to produce a biological active substance that has a strong inhibitory activity against hydrolysis by α-amylase. Extracellular inhibitory substance (inhibitor) from the culture broth of Actinomycetes BY-445 was purified to partial homogeneity by procedures including Amberlite IRA 420 column, Silica gel column, and Sephadex G-50 column chromatography. The partial purifying procedures resulted in 6-fold purification with the overall yield of 21.4% and the velocity of the inhibitor was reached at maximum in inhibitor concentration of 200㎍ against α-amylase enzyme concentration of 100㎍. In effect of metal salt, Co^(+2), Hg^(+2), Pb^(+2) and Cu^(+2), the relative inhibitory activity were shown about 13, 30, 37 and 72%, respectively and the inhibitor was also appeared to be relatively thermostable, and no appreciable inactivation was observed after incubation at 100℃ for 2 hour. The substance was stable in pH range from 2.0 to 12.0 at 37℃. The biochemical reaction of the substance was positive to Anthrone, Molisch and Phenol-sulfuric acid, but negative to Ninhydrin, Biuret and Benedict. A strong inhibitory activity against hydrolysis by α-amylase of human saliva, Bacillus subtilis, and porcine pancreas and glucoamylase of Aspergillus niger was shown 87, 81, 79 and 63%, respectively. but it did not inhibit cellulase of Aspergillus sp. and invertase of Saccaromyces cerevisiae.

우리 나라 학교 환경교육의 제 문제와 개선방안

방진영,김대희 순천대학교 과학교육연구소 2000 科學과 敎育 Vol.8 No.-

Environmental education is very important for the settlement of environmental problems. However, school environmental education is not activated because the national support is very weak in Korea. This demonstration is dealing with various problems and developing methods of school environmental education in Korea. The major developing methods are 1) systematization of school environmental education contents, 2) diversification of environmental education methods, 3) construction of life-long environmental education system. The national financial and administrational supports are essential to realize these methods.