Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

전신마취유도 중 발생한 심방세동과 회복중 발생한 발작성 상심실성 빈맥

방윤식,신동욱,이태규,박정현,길현주,이종연 이화여자대학교 의과학연구소 2012 EMJ (Ewha medical journal) Vol.35 No.2

A healthy 35-year-old man who was scheduled for closed reduction of nasal bone fracture developed atrial fibrillation during induction of general anesthesia after intravenous glycopyrrolate injection. During emergence of general anesthesia, atrial fibrillation was suddenly changed to paroxysmal supraventricular tachycardia with 200 beat per minute and lasted for about 10 seconds. Because blood pressure was stable, esmolol was used to reduce ventricular response. At recovery room, ventricular response reduction about 55 beat per minute was observed after intravenous injection of verapamil 5 mg. Thereafter, the rhythm was returned to normal sinus rhythm with bradycardia.

멀티미디어 통신을 위한 인터넷 용량관리 기능

방기천,장희선,신현철 남서울대학교 공학연구센터 2002 공학연구 Vol.3 No.-

The traffic engineering consists of traffic management, capacity management and network planning. In this paper, we present the capacity management function in traffic engineering for next generation multimedia internet services. The link capacity design, shortest-route design and multiple-time network design model is presented. Finally, day-load variation design model and traffic forecasting/reservation model for internet traffic are introduced.

저위교합 유구치에서의 공간확장술을 이용한 외과적 접근

방석윤,김은정,김현정,김영진,남순현 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.3

저위교합이란 능동적 맹출기 동안이나 이후 해당치아의 교합면 방향으로의 성장이 멈추어 다른 치아들보다 하방에 존재하는 것을 말한다 저위교합치의 결과로 침하치의 만기잔존, 부정교합, 증가된 치아우식 감수성과 치주질환의 가능성, 계승치의 이동 등이 나타날 수 있다. 따라서 조기진단 및 적절한 처치가 필요하다. 저위교합치의 치료는 저위교합 정도나 후속 영구치의 발육정도에 따라 보존에서 발치까지 다양하다. 영구 계승치가 없거나 부분적인 침하치가 치주적으로 건전한 경우, 공간문제를 발생하지 않는 경우 보존적인 접근이 추천되기도 한다. 그러나 계승치가 존재하고 맹출이 지연되는 경우, 국소적인 염증반응과 관련되는 경우 교정적인 치료가 필요한 경우에는 발거가 필요할 수도 있다. 특히 저위교합된 유구치의 상방으로 인접치가 경사되어 악궁장경에 문제를 초래할 위험이 있는 경우 외과적인 발치가 필요할 수도 있으며 경사가 심한 경우 발치를 위한 외과적 접근이 힘들 수 있다. 심하게 경사된 인접치로 인하여 저위교합치의 외과적 발거가 힘들 환아에서 가철성 교정장치를 이용하여 공간확보를 진행 하였으며 보다 용이하게 치아를 발거할 수 있었다. Infraclusion may be defined as teeth that stop their relative occlusal movement in the dental arches during or after the period of active eruption and then remain under the occlusal plane. Delayed exfoliation, malocclusion, increased susceptibility to dental caries and periodontal disease of both the neighboring teeth and retained molar, and dislocation of' the successor are the consequencces of infraclusion of primary molars. Therefore, early diagnosis and appropriate treatments are necessary. The therapeutic approach of' the infracluded teeth varied from preservation to extraction. The teeth with simple infraclusion without any signs of interference with occlusal and jaw development may be examined periodically with fellow-up check and radiographically. However, if the infracluded tooth interferes with normal eruption of successor or shows any sign of delayed resorption, or the tipping of adjacent teeth or supraeruption of op-posing teeth is expected, the teeth inflicted should be extracted and appropriate measures should be provided in order to maintain the normal development of occlusion and dentition. The adjacent teeth which have been collapsed over a infracluded deciduous teeth can disturb the arch length perimeter. In such cases, surgical approach might be necessary, although it would be difficult when teeth are severly leaned. However, an easier surgical access have been obtained by space regaining procedures, in young patients whose arch length has been shortened due to the infracluded teeth.

트레드밀 운동이 일부 흡연 남자대학생의 혈액 성분과 항산화효소에 미치는 영향

방현수 김천대학교 2015 김천대학교 논문집 Vol.36 No.-

Purpose : The purpose of this study was to analyze the changes blood components and antioxidant enzymes of by treadmill exercise in smoking male college students. Method : The subjects were consisted of normal eight smoking male college students. They were from 20 to 25 years old. Treadmill exercise was conducted for 3 weeks and was comprised of 60 minutes for three times a week. All subjects measured blood components variables(Red Blood Cell; RBC, White Blood Cell; WBC, hematocrit, neutrophil), antioxidant enzymes variables(glutathione peroxidase, superoxide dismutase) before and after 3-weeks. Statistics analysis used to paired-t test. Result : The results of this study were summarized as follows: 1. The WBC was increased in the pre-post test(p<.05), but the RBC, hematocrit, neutrophil were not increased in the pre-post test(p>.05). 2. The glutathione peroxidase, superoxide dismutase were increased in the pre-post test(p<.05) Conclusion : In the present study, results indicate that treadmill exercise helps to improve the blood components(WBC) and antioxidant enzymes in smoking male college students.

의류상품의 특성과 문제점 개선안에 관한 연구

방경혜,송민재,이영해,조현애,최문희 연세대학교 생활과학대학 1985 婦學 Vol.18 No.-

Nowdays, proper merchandising system of clothing is needed not only for functional clothes but also for fashionable ones. Since nineteen eighties, ready­to­wear garments have been popular for Korean women. But it is hard to tell whether or not the system of the ready­to­wear industry is concrete because of the short history. The purpose of this study was to investigate the process of the merchandising system of the ready­to­wear garments and to recognize the problem which the system of the garments had have. Furthermore, it aimed to find out the idea or improving the problem for better ready­to­wear garments. This research was done through information obtained from several libraries. And, the period of this was from May of August, 1984. The results of this study were: 1. There was an indivisible relationship between merchandising system and fashion, and the importance of the merchandising­planning was raised. 2. The manufacturers should research and analyze consumers since they are aiming at them in nature. 3. The manufacturers should aim at the invention of the creative designs and the introduction of advanced techniques in producing textiles and their accessaries. 4. There should be some institutes to teach fashion and other areas related to ready­to­wear garments. 5. It was necessary to inform consumers to be able to select proper garments in the market.

창살문과 컴퓨터 그래픽을 활용한 포장용 한지 디자인에 관한 연구

방현지 한국공예학회 1999 조형디자인연구 Vol.2 No.2

The goal of this study is to develop a high sensitive wrapping Hanji design, using computer graphic which is a new expressional tool of the designers, to make effective process of it, and to get variant effect through modern way for suitable wrapping Hanji, with Window-Lattice, among the traditional designs. In this paper, I studied on the meaning of the Window-Lattice, historical research, the uniqueness of the character of the form, the meaning of wrapping paper, and the meaning and character of Hanji, through the literatures and references. And, I could imagine today's situation of the design of Hanji, through the nowaday's situation of the design of Hanji for wrapping. And, I suggested effective and scientific ways through the design, using computer graphic, constructing factors, and the way for arrangement. And, I studied on the practical way, through real market, on the background of these theories. The Hanji design, which is developed by this study, is a solution of environment problem, and, at the same time, I suggested this to make a opportunity for higher value of the products, by satisfying human emotional desire to be back to the nature. The computer graphic, which is used fro higher efficiency of the design, can change its shape and color, only with basic unit design, so we can save time and money, and it was possible for me to expect good result by making trial products.

동국의대에서 실시한 2002년 전염병관리실무과정에 대한 평가

방미란,임현술,정해관,한영란,정철 대한보건협회 2003 대한보건연구 Vol.29 No.1

Communicable diseases have recently reemerged, with new communicable diseases continually breakmg out on a global scale; threaten the health and economics of populations. The Korean National Institute of Health carries out Field Management Training Prcgrams (FMTP) to address this problem The objectives of this study were the evaluation of the understanding and satisfaction for this program, and recommendation for improvement strategies. We conducted a questionnaire survey of 80 persons who completed the FMTP, at Dongguk University, between March 1 and November 30, 2002. The data collected was analyzed by Student s t-statistics. The understanding of the curriculum was Well in 42 (52.5%) and Moderate in 24 persons (30.0%). The satisfaction with the curriculum was Moderate in 38 (47.5%) and Good in 30 persons (37.5%). The satisfaction for the period of education was Bad for 31 persons (38.7%), which accounted for the majority of cases. The mean scores, by Likert s scale, for the understanding and satisfaction of the curriculum were 3.70 (±0.71) and 3.32 (i0.721, respectively. The mean score for the epidemiological survey was the highest for satisfaction with the course, at 3.78 (±0.62). The mean value of satisfaction for curriculum in education of period was significantly higher in health department group than in nursing department group (P<0.05). From this survey it appears the respondents were satisfied with this program. The FMTP will be systematically revised for curriculums, and effort will be required to develop controls for communicable disease, both by the government and the university.

과실책임론의 경향에 대한 소고

윤방현 대불대학교 1998 論文集 Vol.4 No.1

Ascorbic Acid와 Pyrophosphate로부터 Ascorbic Acid-2-Phosphate의 효소적 생산

최현일,이상협,방원기 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

Ascorbic acid (AsA)와 pyrophosphate로부터 ascorbic acid-2-phosphate (AsA2P)를 생산할 수 있는 미생물을 본 실험실에서 분리한 보존 균주로부터 선별하였다. 선별 균주 중에서 Cellulomonas sp. AP-7이 가장 높은 AsA2P 생산능을 나타내었다. 효소원으로 본 균주의 무세포 추출물을 이용하여 AsA와 PPi로부터 AsA2P의 최적 생산 조건을 조사하였다. AsA2P의 최적 생산을 위한 반응 혼합액은 효소원으로 21 mg/ml의 무세포 추출물, 150 mM sodium acetate 완충액(pH 4.5), 기질로 250 mM AsA 그리고 인산 공여체로 200 mM PPi로 조성되었으며, 이 반응 혼합액을 사용하여 33℃에서 48시간 반응시켰을 때, 31.9 mM의 AsA2P가 생산되었으며, 이는 반응에 사용한 AsA에 대해 12.76%의 수율에 해당하였다. Microorganisms capable of producing ascorbic acid-2-phosphate (AsA2P) from ascorbic acid (AsA) and pyrophosphate (PPi) wee screened from the culture collection of this laboratory. Among them Cellulomonas sp. AP-7 showed the highest productivity of AsA2P. The optimal conditions for the production of AsA2P from AsA and PPi with cell-free extract as an enzyme source wee investigated. The reaction mixture for the maximal production of AsA2P consisted of 21 g protein of cell-free extract per liter as the enzyme source, 250 mM AsA, 200 mM sodium pyrophosphate, 150 mM sodium acetate buffer (pH 4.5). By using this reaction mixture, 31.9 mM of AsA2P, which corresponded to a 12.76% yield based on AsA, was produced after incubation of 48 hr at 33℃.